Skip to main content
Journal cover image

Insight into F plasmid DNA segregation revealed by structures of SopB and SopB-DNA complexes.

Publication ,  Journal Article
Schumacher, MA; Piro, KM; Xu, W
Published in: Nucleic Acids Res
July 2010

Accurate DNA segregation is essential for genome transmission. Segregation of the prototypical F plasmid requires the centromere-binding protein SopB, the NTPase SopA and the sopC centromere. SopB displays an intriguing range of DNA-binding properties essential for partition; it binds sopC to form a partition complex, which recruits SopA, and it also coats DNA to prevent non-specific SopA-DNA interactions, which inhibits SopA polymerization. To understand the myriad functions of SopB, we determined a series of SopB-DNA crystal structures. SopB does not distort its DNA site and our data suggest that SopB-sopC forms an extended rather than wrapped partition complex with the SopA-interacting domains aligned on one face. SopB is a multidomain protein, which like P1 ParB contains an all-helical DNA-binding domain that is flexibly attached to a compact (beta(3)-alpha)(2) dimer-domain. Unlike P1 ParB, the SopB dimer-domain does not bind DNA. Moreover, SopB contains a unique secondary dimerization motif that bridges between DNA duplexes. Both specific and non-specific SopB-DNA bridging structures were observed. This DNA-linking function suggests a novel mechanism for in trans DNA spreading by SopB, explaining how it might mask DNA to prevent DNA-mediated inhibition of SopA polymerization.

Duke Scholars

Published In

Nucleic Acids Res

DOI

EISSN

1362-4962

Publication Date

July 2010

Volume

38

Issue

13

Start / End Page

4514 / 4526

Location

England

Related Subject Headings

  • Protein Structure, Tertiary
  • Protein Multimerization
  • Nucleic Acid Conformation
  • Models, Molecular
  • F Factor
  • Developmental Biology
  • DNA-Binding Proteins
  • DNA, Bacterial
  • Centromere
  • Binding Sites
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Schumacher, M. A., Piro, K. M., & Xu, W. (2010). Insight into F plasmid DNA segregation revealed by structures of SopB and SopB-DNA complexes. Nucleic Acids Res, 38(13), 4514–4526. https://doi.org/10.1093/nar/gkq161
Schumacher, Maria A., Kevin M. Piro, and Weijun Xu. “Insight into F plasmid DNA segregation revealed by structures of SopB and SopB-DNA complexes.Nucleic Acids Res 38, no. 13 (July 2010): 4514–26. https://doi.org/10.1093/nar/gkq161.
Schumacher MA, Piro KM, Xu W. Insight into F plasmid DNA segregation revealed by structures of SopB and SopB-DNA complexes. Nucleic Acids Res. 2010 Jul;38(13):4514–26.
Schumacher, Maria A., et al. “Insight into F plasmid DNA segregation revealed by structures of SopB and SopB-DNA complexes.Nucleic Acids Res, vol. 38, no. 13, July 2010, pp. 4514–26. Pubmed, doi:10.1093/nar/gkq161.
Schumacher MA, Piro KM, Xu W. Insight into F plasmid DNA segregation revealed by structures of SopB and SopB-DNA complexes. Nucleic Acids Res. 2010 Jul;38(13):4514–4526.
Journal cover image

Published In

Nucleic Acids Res

DOI

EISSN

1362-4962

Publication Date

July 2010

Volume

38

Issue

13

Start / End Page

4514 / 4526

Location

England

Related Subject Headings

  • Protein Structure, Tertiary
  • Protein Multimerization
  • Nucleic Acid Conformation
  • Models, Molecular
  • F Factor
  • Developmental Biology
  • DNA-Binding Proteins
  • DNA, Bacterial
  • Centromere
  • Binding Sites