Scholars@Duke

• Background
• 

  Education, Training, & Certifications

  • Ph.D., Oregon Health and Science University 1995
• 

  Duke Appointment History

  • Associate Professor of Biochemistry, Biochemistry, Basic Science Departments 2011 - 2015
  • Instructor in the Department of Biochemistry, Biochemistry, Basic Science Departments 2011
• Recognition
• 

  In the News

  • SEP 6, 2017

    Molecular Map Shows How to Disable Dangerous Bioweapon

  • JUL 29, 2015

    Structures Reveal Basis of Recurring Urinary Tract Infections

  • MAR 10, 2015

    Schumacher elected to American Academy of Microbiology

  • AUG 28, 2014

    Small Molecule Acts As On-Off Switch for Nature's Antibiotic Factory

  • JUN 9, 2014

    Protein Could Put Antibiotic-Resistant Bugs in Handcuffs
• 

  Awards & Honors

  • AAAS Fellows. American Association for the Advancement of Science, The. 2012
• Research
• 

  Selected Grants

  • Cell signaling through O-GlcNAc reader proteins awarded by National Institutes of Health 2016 - 2021
  • Complete atomic dissection of the B. subtilis nitrogen regulatory pathway awarded by National Institutes of Health 2015 - 2019
  • Mechanism of cofactor biosynthesis required for chronic bacterial infection awarded by National Institutes of Health 2015 - 2019
  • Assembly and partition mechanism of Walker-box based segregation machinery awarded by National Institutes of Health 2015 - 2019
  • Structural Biology and Biophysics Training Program awarded by National Institutes of Health 1994 - 2015
  • Structural mechanism of DNA segregation by the pSK41 par system awarded by National Institutes of Health 2009 - 2012
• Publications & Artistic Works
• 

  Selected Publications

  • Journal Articles

    • Schumacher, MA, den Hengst, CD, Bush, MJ, Le, TBK, Tran, NT, Chandra, G, Zeng, W, Travis, B, Brennan, RG, and Buttner, MJ. "The MerR-like protein BldC binds DNA direct repeats as cooperative multimers to regulate Streptomyces development." Nature communications 9, no. 1 (March 19, 2018): 1139-.  Full Text
    • Cuthbert, BJ, Ross, W, Rohlfing, AE, Dove, SL, Gourse, RL, Brennan, RG, and Schumacher, MA. "Dissection of the molecular circuitry controlling virulence in Francisella tularensis." Genes & development 31, no. 15 (August 2017): 1549-1560.  Full Text
    • Schumacher, MA, Zeng, W, Findlay, KC, Buttner, MJ, Brennan, RG, and Tschowri, N. "The Streptomyces master regulator BldD binds c-di-GMP sequentially to create a functional BldD2-(c-di-GMP)4 complex." Nucleic acids research 45, no. 11 (June 2017): 6923-6933.  Full Text
    • McLeod, BN, Allison-Gamble, GE, Barge, MT, Tonthat, NK, Schumacher, MA, Hayes, F, and Barillà, D. "A three-dimensional ParF meshwork assembles through the nucleoid to mediate plasmid segregation." Nucleic acids research 45, no. 6 (April 2017): 3158-3171.  Full Text
    • Schumacher, MA, Huang, K-H, Zeng, W, and Janakiraman, A. "Structure of the Z Ring-associated Protein, ZapD, Bound to the C-terminal Domain of the Tubulin-like Protein, FtsZ, Suggests Mechanism of Z Ring Stabilization through FtsZ Cross-linking." The Journal of biological chemistry 292, no. 9 (March 2017): 3740-3750.  Full Text
    • Zhang, H, and Schumacher, MA. "Structures of partition protein ParA with nonspecific DNA and ParB effector reveal molecular insights into principles governing Walker-box DNA segregation." Genes & development 31, no. 5 (March 2017): 481-492.  Full Text
    • Schumacher, MA. "Bacterial Nucleoid Occlusion: Multiple Mechanisms for Preventing Chromosome Bisection During Cell Division." Sub-cellular biochemistry 84 (January 2017): 267-298.  Full Text
    • Schumacher, MA, and Zeng, W. "Structures of the activator of K. pneumonia biofilm formation, MrkH, indicates PilZ domains involved in c-di-GMP and DNA binding." Proceedings of the National Academy of Sciences of the United States of America 113, no. 36 (September 2016): 10067-10072.  Full Text
    • Schumacher, MA, Lee, J, and Zeng, W. "Molecular insights into DNA binding and anchoring by the Bacillus subtilis sporulation kinetochore-like RacA protein." Nucleic acids research 44, no. 11 (June 2016): 5438-5449.  Full Text
    • Schumacher, MA, and Zeng, W. "Structures of the nucleoid occlusion protein SlmA bound to DNA and the C-terminal domain of the cytoskeletal protein FtsZ." Proceedings of the National Academy of Sciences of the United States of America 113, no. 18 (May 2016): 4988-4993.  Full Text
    • Tonthat, NK, Juvvadi, PR, Zhang, H, Lee, SC, Venters, R, Spicer, L, Steinbach, WJ, Heitman, J, and Schumacher, MA. "Structures of Pathogenic Fungal FKBP12s Reveal Possible Self-Catalysis Function." mBio 7, no. 2 (April 26, 2016): e00492-e00416.  Full Text
    • Schumacher, MA, Zeng, W, Huang, K-H, Tchorzewski, L, and Janakiraman, A. "Structural and Functional Analyses Reveal Insights into the Molecular Properties of the Escherichia coli Z Ring Stabilizing Protein, ZapC." The Journal of biological chemistry 291, no. 5 (January 2016): 2485-2498.  Full Text
    • Lee, SC, Li, A, Calo, S, Inoue, M, Tonthat, NK, Bain, JM, Louw, J, Shinohara, ML, Erwig, LP, Schumacher, MA, Ko, DC, and Heitman, J. "Calcineurin orchestrates dimorphic transitions, antifungal drug responses and host-pathogen interactions of the pathogenic mucoralean fungus Mucor circinelloides." Molecular microbiology 97, no. 5 (September 2015): 844-865.  Full Text
    • Schumacher, MA, Tonthat, NK, Lee, J, Rodriguez-Castañeda, FA, Chinnam, NB, Kalliomaa-Sanford, AK, Ng, IW, Barge, MT, Shaw, PLR, and Barillà, D. "Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages." Science (New York, N.Y.) 349, no. 6252 (September 2015): 1120-1124.  Full Text
    • Schumacher, MA, Balani, P, Min, J, Chinnam, NB, Hansen, S, Vulić, M, Lewis, K, and Brennan, RG. "HipBA-promoter structures reveal the basis of heritable multidrug tolerance." Nature 524, no. 7563 (August 2015): 59-64.  Full Text
    • Shaw, PLR, McAdams, NM, Hast, MA, Ammerman, ML, Read, LK, and Schumacher, MA. "Structures of the T. brucei kRNA editing factor MRB1590 reveal unique RNA-binding pore motif contained within an ABC-ATPase fold." Nucleic acids research 43, no. 14 (August 2015): 7096-7109.  Full Text
    • Hover, BM, Tonthat, NK, Schumacher, MA, and Yokoyama, K. "Mechanism of pyranopterin ring formation in molybdenum cofactor biosynthesis." Proceedings of the National Academy of Sciences of the United States of America 112, no. 20 (May 4, 2015): 6347-6352.  Full Text
    • Schumacher, MA, Chinnam, NB, Cuthbert, B, Tonthat, NK, and Whitfill, T. "Structures of regulatory machinery reveal novel molecular mechanisms controlling B. subtilis nitrogen homeostasis." Genes & development 29, no. 4 (February 2015): 451-464.  Full Text
    • Cuthbert, BJ, Brennan, RG, and Schumacher, MA. "Structural and Biochemical Characterization of the Francisella tularensis Pathogenicity Regulator, Macrophage Locus Protein A (MglA)." PloS one 10, no. 6 (January 2015): e0128225-.  Full Text
    • Emptage, RP, Tonthat, NK, York, JD, Schumacher, MA, and Zhou, P. "Structural basis of lipid binding for the membrane-embedded tetraacyldisaccharide-1-phosphate 4'-kinase LpxK." The Journal of biological chemistry 289, no. 35 (August 2014): 24059-24068.  Full Text
    • Tschowri, N, Schumacher, MA, Schlimpert, S, Chinnam, NB, Findlay, KC, Brennan, RG, and Buttner, MJ. "Tetrameric c-di-GMP mediates effective transcription factor dimerization to control Streptomyces development." Cell 158, no. 5 (August 2014): 1136-1147.  Full Text
    • Schumacher, MA, Tonthat, NK, Kwong, SM, Chinnam, NB, Liu, MA, Skurray, RA, and Firth, N. "Mechanism of staphylococcal multiresistance plasmid replication origin assembly by the RepA protein." Proceedings of the National Academy of Sciences of the United States of America 111, no. 25 (June 9, 2014): 9121-9126.  Full Text
    • Daniels, KG, Tonthat, NK, McClure, DR, Chang, Y-C, Liu, X, Schumacher, MA, Fierke, CA, Schmidler, SC, and Oas, TG. "Ligand concentration regulates the pathways of coupled protein folding and binding." J Am Chem Soc 136, no. 3 (January 22, 2014): 822-825.  Full Text  Link to Item
    • Murray, DS, Chinnam, N, Tonthat, NK, Whitfill, T, Wray, LV, Fisher, SH, and Schumacher, MA. "Structures of the Bacillus subtilis glutamine synthetase dodecamer reveal large intersubunit catalytic conformational changes linked to a unique feedback inhibition mechanism." Journal of Biological Chemistry 288, no. 50 (December 13, 2013): 35801-35811.  Full Text
    • Schumacher, MA, Chinnam, N, Ohashi, T, Shah, RS, and Erickson, HP. "The structure of irisin reveals a novel intersubunit β-sheet fibronectin type III (FNIII) dimer: implications for receptor activation." J Biol Chem 288, no. 47 (November 22, 2013): 33738-33744.  Full Text  Open Access Copy  Link to Item
    • Ni, L, Tonthat, NK, Chinnam, N, and Schumacher, MA. "Structures of the Escherichia coli transcription activator and regulator of diauxie, XylR: an AraC DNA-binding family member with a LacI/GalR ligand-binding domain." Nucleic Acids Res 41, no. 3 (February 1, 2013): 1998-2008.  Full Text  Link to Item
    • Tonthat, NK, Milam, SL, Chinnam, N, Whitfill, T, Margolin, W, and Schumacher, MA. "SlmA forms a higher-order structure on DNA that inhibits cytokinetic Z-ring formation over the nucleoid (Proceedings of the National Academy of Sciences of the United States of America (2013) 110, 26, (10586-10591) DOI: 10.1073/pnas.1221036110)." Proceedings of the National Academy of Sciences of the United States of America 110, no. 37 (2013): 15163--.  Full Text
    • Tonthat, NK, Milam, SL, Chinnam, N, Whitfill, T, Margolin, W, and Schumacher, MA. "SlmA forms a higher-order structure on DNA that inhibits cytokinetic Z-ring formation over the nucleoid." Proceedings of the National Academy of Sciences of the United States of America 110, no. 26 (2013): 10586-10591.  Full Text
    • Schumacher, MA, Min, J, Link, TM, Guan, Z, Xu, W, Ahn, Y-H, Soderblom, EJ, Kurie, JM, Evdokimov, A, Moseley, MA, Lewis, K, and Brennan, RG. "Role of unusual P loop ejection and autophosphorylation in HipA-mediated persistence and multidrug tolerance." Cell Rep 2, no. 3 (September 27, 2012): 518-525.  Full Text  Link to Item
    • Schumacher, MA, Ye, Q, Barge, MT, Zampini, M, Barillà, D, and Hayes, F. "Structural mechanism of ATP-induced polymerization of the partition factor ParF: implications for DNA segregation." J Biol Chem 287, no. 31 (July 27, 2012): 26146-26154.  Full Text  Link to Item
    • Schumacher, MA. "Bacterial plasmid partition machinery: a minimalist approach to survival." Curr Opin Struct Biol 22, no. 1 (February 2012): 72-79. (Review)  Full Text  Link to Item
    • Dobruk-Serkowska, A, Caccamo, M, Rodríguez-Castañeda, F, Wu, M, Bryce, K, Ng, I, Schumacher, MA, Barillà, D, and Hayes, F. "Uncoupling of nucleotide hydrolysis and polymerization in the ParA protein superfamily disrupts DNA segregation dynamics." Journal of Biological Chemistry 287, no. 51 (2012): 42545-42553.  Full Text
    • Dupaigne, P, Tonthat, NK, Espéli, O, Whitfill, T, Boccard, F, and Schumacher, MA. "Molecular Basis for a Protein-Mediated DNA-Bridging Mechanism that Functions in Condensation of the E. coli Chromosome." Molecular Cell 48, no. 4 (2012): 560-571.  Full Text
    • Hansen, S, Vulić, M, Min, J, Yen, T-J, Schumacher, MA, Brennan, RG, and Lewis, K. "Regulation of the Escherichia coli HipBA toxin-antitoxin system by proteolysis." PLoS ONE 7, no. 6 (2012).  Full Text
    • Gu, D, Tonthat, NK, Lee, M, Ji, H, Bhat, KP, Hollingsworth, F, Aldape, KD, Schumacher, MA, Zwaka, TP, and McCrea, PD. "Caspase-3 cleavage links δ-catenin to the novel nuclear protein ZIFCAT." Journal of Biological Chemistry 286, no. 26 (2011): 23178-23188.  Full Text
    • Le, TBK, Schumacher, MA, Lawson, DM, Brennan, RG, and Buttner, MJ. "The crystal structure of the TetR family transcriptional repressor SimR bound to DNA and the role of a flexible N-terminal extension in minor groove binding." Nucleic Acids Research 39, no. 21 (2011): 9433-9447.  Full Text
    • Peters, KM, Brooks, BE, Schumacher, MA, Skurray, RA, Brennan, RG, and Brown, MH. "A single acidic residue can guide binding site selection but does not govern QaCR cationic-drug affinity." PLoS ONE 6, no. 1 (2011).  Full Text
    • Schumacher, MA, Sprehe, M, Bartholomae, M, Hillen, W, and Brennan, RG. "Structures of carbon catabolite protein A-(HPr-Ser46-P) bound to diverse catabolite response element sites reveal the basis for high-affinity binding to degenerate DNA operators." Nucleic Acids Research 39, no. 7 (2011): 2931-2942.  Full Text
    • Takeda, K, Tonthat, NK, Glover, T, Xu, W, Koonin, EV, Yanagida, M, and Schumacher, MA. "Implications for proteasome nuclear localization revealed by the structure of the nuclear proteasome tether protein Cut8." Proceedings of the National Academy of Sciences of the United States of America 108, no. 41 (2011): 16950-16955.  Full Text
    • Tonthat, NK, Arold, ST, Pickering, BF, Dyke, MWV, Liang, S, Lu, Y, Beuria, TK, Margolin, W, and Schumacher, MA. "Molecular mechanism by which the nucleoid occlusion factor, SlmA, keeps cytokinesis in check." EMBO Journal 30, no. 1 (2011): 154-164.  Full Text
    • Ni, L, Xu, W, Kumaraswami, M, and Schumacher, MA. "Plasmid protein TubR uses a distinct mode of HTH-DNA binding and recruits the prokaryotic tubulin homolog TubZ to effect DNA partition." Proceedings of the National Academy of Sciences of the United States of America 107, no. 26 (2010): 11763-11768.  Full Text
    • Popp, D, Xu, W, Narita, A, Brzoska, AJ, Skurray, RA, Firth, N, Ghoshdastider, U, Maéda, Y, Robinson, RC, and Schumacher, MA. "Structure and filament dynamics of the pSK41 actin-like ParM protein. Implications for plasmid DNA segregation (Journal of Biological Chemistry (2010) 285, (10130-10140) DOI: 10.1074/jbc.A109.071613)." Journal of Biological Chemistry 285, no. 23 (2010): 18122--.
    • Popp, D, Xu, W, Narita, A, Brzoska, AJ, Skurray, RA, Firth, N, Goshdastider, U, Maéda, Y, Robinson, RC, and Schumacher, MA. "Structure and filament dynamics of the pSK41 actin-like ParM protein implications for plasmid DNA segregation." Journal of Biological Chemistry 285, no. 13 (2010): 10130-10140.  Full Text
    • Schumacher, MA, Piro, KM, and Xu, W. "Insight into F plasmid DNA segregation revealed by structures of SopB and SopB-DNA complexes." Nucleic Acids Research 38, no. 13 (2010): 4514-4526.  Full Text
    • Sprehe, M, Fisk, JC, McEvoy, SM, Read, LK, and Schumacher, MA. "Structure of the Trypanosoma brucei p22 protein, a cytochrome oxidase subunit II-specific RNA-editing accessory factor." Journal of Biological Chemistry 285, no. 24 (2010): 18899-18908.  Full Text
    • Dunham, TD, Xu, W, Funnell, BE, and Schumacher, MA. "Structural basis for ADP-mediated transcriptional regulation by P1 and P7 ParA." EMBO Journal 28, no. 12 (2009): 1792-1802.  Full Text
    • Ni, L, Jensen, SO, Tonthat, NK, Berg, T, Kwong, SM, Guan, FHX, Brown, MH, Skurray, RA, Firth, N, and Schumacher, MA. "The Staphylococcus aureus pSK41 plasmid-encoded ArtA protein is a master regulator of plasmid transmission genes and contains a RHH motif used in alternate DNA-binding modes." Nucleic Acids Research 37, no. 20 (2009): 6970-6983.  Full Text
    • Schumacher, MA, Piro, KM, Xu, W, Hansen, S, Lewis, K, and Brennan, RG. "Molecular mechanisms of HipA-mediated multidrug tolerance and its neutralization by HipB." Science 323, no. 5912 (2009): 396-401.  Full Text
    • Boudko, SP, Engel, J, Okuyama, K, Mizuno, K, Bächinger, HP, and Schumacher, MA. "Crystal structure of human type III collagen Gly⁹⁹¹-Gly ¹⁰³² cystine knot-containing peptide shows both 7/2 and 10/3 triple helical symmetries." Journal of Biological Chemistry 283, no. 47 (2008): 32580-32589.  Full Text
    • Peters, KM, Schuman, JT, Skurray, RA, Brown, MH, Brennan, RG, and Schumacher, MA. "QacR-cation recognition is mediated by a redundancy of residues capable of charge neutralization." Biochemistry 47, no. 31 (2008): 8122-8129.  Full Text
    • Schumacher, MA. "Structural biology of plasmid partition: Uncovering the molecular mechanisms of DNA segregation." Biochemical Journal 412, no. 1 (2008): 1-18.  Full Text
    • Wilkinson, DS, Tsai, W-W, Schumacher, MA, and Barton, MC. "Chromatin-bound p53 anchors activated Smads and the mSin3A corepressor to confer transforming growth factor β-mediated transcription repression." Molecular and Cellular Biology 28, no. 6 (2008): 1988-1998.  Full Text
    • Zíková, A, Kopečná, J, Schumacher, MA, Stuart, K, Trantírek, L, and Lukeš, J. "Structure and function of the native and recombinant mitochondrial MRP1/MRP2 complex from Trypanosoma brucei." International Journal for Parasitology 38, no. 8-9 (2008): 901-912.  Full Text
    • Schumacher, MA, Glover, TC, Brzoska, AJ, Jensen, SO, Dunham, TD, Skurray, RA, and Firth, N. "Segrosome structure revealed by a complex of ParR with centromere DNA." Nature 450, no. 7173 (2007): 1268-1271.  Full Text
    • Schumacher, MA, Mansoor, A, and Funnell, BE. "Structure of a four-way bridged ParB-DNA complex provides insight into P1 segrosome assembly." Journal of Biological Chemistry 282, no. 14 (2007): 10456-10464.  Full Text
    • Schumacher, MA, Seidel, G, Hillen, W, and Brennan, RG. "Structural Mechanism for the Fine-tuning of CcpA Function by The Small Molecule Effectors Glucose 6-Phosphate and Fructose 1,6-Bisphosphate." Journal of Molecular Biology 368, no. 4 (2007): 1042-1050.  Full Text
    • Schumacher, MA. "Structural biology of plasmid segregation proteins." Current Opinion in Structural Biology 17, no. 1 (2007): 103-109.  Full Text
    • Vecchiarelli, AG, Schumacher, MA, and Funnell, BE. "P1 partition complex assembly involves several modes of protein-DNA recognition." Journal of Biological Chemistry 282, no. 15 (2007): 10944-10952.  Full Text
    • Schumacher, MA, Karamooz, E, Zíková, A, Trantírek, L, and Lukeš, J. "Crystal Structures of T. brucei MRP1/MRP2 Guide-RNA Binding Complex Reveal RNA Matchmaking Mechanism." Cell 126, no. 4 (2006): 701-711.  Full Text
    • Schumacher, MA, Mizuno, K, and Bächinger, HP. "The crystal structure of a collagen-like polypeptide with 3(S)-hydroxyproline residues in the Xaa position forms a standard 7/2 collagen triple helix." Journal of Biological Chemistry 281, no. 37 (2006): 27566-27574.  Full Text
    • Schumacher, MA, Seidel, G, Hillen, W, and Brennan, RG. "Phosphoprotein Crh-Ser⁴⁶-P displays altered binding to CcpA to effect carbon catabolite regulation." Journal of Biological Chemistry 281, no. 10 (2006): 6793-6800.  Full Text
    • Schumacher, M, Mizuno, K, and Bächinger, HP. "The crystal structure of the collagen-like polypeptide (Glycyl-4(R)- hydroxyprolyl-4(R)-hydroxyprolyl)₉ at 1.55 Å resolution shows up-puckering of the proline ring in the Xaa position." Journal of Biological Chemistry 280, no. 21 (2005): 20397-20403.  Full Text
    • Schumacher, MA, and Funnell, BE. "Structures of ParB bound to DNA reveal mechanism of partition complex formation." Nature 438, no. 7067 (2005): 516-519.  Full Text
    • Murray, DS, Schumacher, MA, and Brennan, RG. "Crystal Structures of QacR-Diamidine Complexes Reveal Additional Multidrug-binding Modes and A Novel Mechanism of Drug Charge Neutralization." Journal of Biological Chemistry 279, no. 14 (2004): 14365-14371.  Full Text
    • Schumacher, MA, Allen, GS, Diel, M, Seidel, G, Hillen, W, and Brennan, RG. "Structural basis for allosteric control of the transcription regulator CcpA by the phosphoprotein HPr-Ser46-P." Cell 118, no. 6 (2004): 731-741.  Full Text
    • Schumacher, MA, Crum, M, and Miller, MC. "Crystal structures of apocalmodulin and an apocalmodulin/SK potassium channel gating domain complex." Structure 12, no. 5 (2004): 849-860.  Full Text
    • Schumacher, MA, Miller, MC, and Brennan, RG. "Structural mechanism of the simultaneous binding of two drugs to a multidrug-binding protein." EMBO Journal 23, no. 15 (2004): 2923-2930.  Full Text
    • Schumacher, MA, Lau, AOT, and Johnson, PJ. "Structural Basis of Core Promoter Recognition in a Primitive Eukaryote." Cell 115, no. 4 (2003): 413-424.  Full Text
    • Schumacher, MA, and Brennan, RG. "Deciphering the molecular basis of multidrug recognition: Crystal structures of the Staphylococcus aureus multidrug binding transcription regulator QacR." Research in Microbiology 154, no. 2 (2003): 69-77.  Full Text
    • Bruening-Wright, A, Schumacher, MA, Adelman, JP, and Maylie, J. "Localization of the activation gate for small conductance Ca²⁺-activated K⁺ channels." Journal of Neuroscience 22, no. 15 (2002): 6499-6506.
    • Schumacher, M, and Adelman, JP. "Ion channels: An open and shut case." Nature 417, no. 6888 (2002): 501-502.  Full Text
    • Schumacher, MA, Bashor, CJ, Minsun, HS, Otsu, K, Zhu, S, Parry, RJ, Ullman, B, and Brennan, RG. "The structural mechanism of GTP stabilized oligomerization and catalytic activation of the Toxoplasma gondii uracil phosphoribosyltransferase." Proceedings of the National Academy of Sciences of the United States of America 99, no. 1 (2002): 78-83.  Full Text
    • Schumacher, MA, Miller, MC, Grkovic, S, Brown, MH, Skurray, RA, and Brennan, RG. "Erratum: Structural basis for cooperative DNA binding by two dimers of the multidrug-binding protein QacR (EMBO Journal (2002) 21 (1210-1218))." EMBO Journal 21, no. 9 (2002): 2301--.  Full Text
    • Schumacher, MA, Miller, MC, Grkovic, S, Brown, MH, Skurray, RA, and Brennan, RG. "Structural basis for cooperative DNA binding by two dimers of the multidrug-binding protein QacR." EMBO Journal 21, no. 5 (2002): 1210-1218.  Full Text
    • Schumacher, MA, Pearson, RF, Møller, T, Valentin-Hansen, P, and Brennan, RG. "Structures of the pleiotropic translational regulator Hfq and an Hfq-RNA complex: A bacterial Sm-like protein." EMBO Journal 21, no. 13 (2002): 3546-3556.  Full Text
    • Schumacher, MA, Todd, JL, Rice, AE, Tanner, KG, and Denu, JM. "Structural basis for the recognition of a bisphosphorylated MAP kinase peptide by human VHR protein phosphatase." Biochemistry 41, no. 9 (2002): 3009-3017.  Full Text
    • Schumacher, MA, and Brennan, RG. "Structural mechanisms of multidrug recognition and regulation by bacterial multidrug transcription factors." Molecular Microbiology 45, no. 4 (2002): 885-893.  Full Text
    • Craig, JC, Schumacher, MA, Mansoor, SE, Farrens, DL, Brennan, RG, and Goodman, RH. "Consensus and Variant cAMP-regulated Enhancers Have Distinct CREB-binding Properties." Journal of Biological Chemistry 276, no. 15 (2001): 11719-11728.  Full Text
    • Grkovic, S, Brown, MH, Schumacher, MA, Brennan, RG, and Skurray, RA. "The staphylococcal QacR multidrug regulator binds a correctly spaced operator as a pair of dimers." Journal of Bacteriology 183, no. 24 (2001): 7102-7109.  Full Text
    • Schumacher, MA, Hurlburt, BK, and Brennan, RG. "Erratum: Crystal structures of SarA, a pleiotropic regulator of virulence genes in S. aureus (Nature (20010 409 (215-219))." Nature 414, no. 6859 (2001): 85--.
    • Schumacher, MA, Hurlburt, BK, and Brennan, RG. "Crystal structures of SarA, a pleiotropic regulator of virulence genes in S. aureus." Nature 409, no. 6817 (2001): 215-219.  Full Text
    • Schumacher, MA, Miller, MC, Grkovic, S, Brown, MH, Skurray, RA, and Brennan, RG. "Structural mechanisms of QacR induction and multidrug recognition." Science 294, no. 5549 (2001): 2158-2163.  Full Text
    • Schumacher, MA, Rivard, AF, Bächinger, HP, and Adelman, JP. "Structure of the gating domain of a Ca²⁺-activated K⁺ channel complexed with Ca²⁺/calmodulin." Nature 410, no. 6832 (2001): 1120-1124.  Full Text
    • Brown, M, Schumacher, MA, Wiens, GD, Brennan, RG, and Rittenberg, MB. "The structural basis of repertoire shift in an immune response to phosphocholine." Journal of Experimental Medicine 191, no. 12 (2000): 2101-2111.  Full Text
    • Schumacher, MA, Goodman, RH, and Brennan, RG. "The structure of a CREB bZIP·somatostatin CRE complex reveals the basis for selective dimerization and divalent cation-enhanced DNA binding." Journal of Biological Chemistry 275, no. 45 (2000): 35242-35247.  Full Text
    • Schumacher, MA, Scott, DM, Mathews, II, Ealick, SE, Roos, DS, Ullman, B, and Brennan, RG. "Crystal structures of Toxoplasma gondii adenosine kinase reveal a novel catalytic mechanism prodrug binding." Journal of Molecular Biology 298, no. 5 (2000): 875-893.  Full Text
    • Schumacher, MA, Scott, DM, Mathews, II, Ealick, SE, Roos, DS, Ullman, B, and Brennan, RG. "Crystal structures of Toxoplasma gondii adenosine kinase reveal a novel catalytic mechanism and prodrug binding." Journal of Molecular Biology 296, no. 2 (2000): 549-567.  Full Text
    • Glasfeld, A, Koehler, AN, Schumacher, MA, and Brennan, RG. "The role of lysine 55 in determining the specificity of the purine repressor for its operators through minor groove interactions." Journal of Molecular Biology 291, no. 2 (1999): 347-361.  Full Text
    • Rechtin, TM, Gillaspy, AF, Schumacher, MA, Brennan, RG, Smeltzer, MS, and Hurlburt, BK. "Characterization of the SarA virulence gene regulator of staphylococcus aureus." Molecular Microbiology 33, no. 2 (1999): 307-316.  Full Text
    • Lu, F, Schumacher, MA, Arvidson, DN, Haldimann, A, Wanner, BL, Zalkin, H, and Brennan, RG. "Structure-based redesign of corepressor specificity of the Escherichia coli purine repressor by substitution of residue 190." Biochemistry 37, no. 4 (1998): 971-982.  Full Text
    • Schumacher, MA, Carter, D, Scott, DM, Roos, DS, Ullman, B, and Brennan, RG. "Crystal structures of Toxoplasma gondii uracil phosphoribosyltransferase reveal the atomic basis of pyrimidine discrimination and prodrug binding." EMBO Journal 17, no. 12 (1998): 3219-3232.  Full Text
    • Schumacher, MA, Glasfeld, A, Zalkin, H, and Brennan, RG. "The x-ray structure of the PurR-guanine-purF operator complex reveals the contributions of complementary electrostatic surfaces and a water- mediated hydrogen bond to corepressor specificity and binding affinity." Journal of Biological Chemistry 272, no. 36 (1997): 22648-22653.  Full Text
    • Schumacher, MA, Zheleznova, EE, Poundstone, KS, Kluger, R, Jones, RT, and Brennan, RG. "Allosteric intermediates indicate R2 is the liganded hemoglobin end state." Proceedings of the National Academy of Sciences of the United States of America 94, no. 15 (1997): 7841-7844.  Full Text
    • Glasfeld, A, Schumacher, MA, Choi, K-Y, Zalkin, H, and Brennan, RG. "A positively charged residue bound in the minor groove does not alter the bending of a DNA duplex." Journal of the American Chemical Society 118, no. 51 (1996): 13073-13074.  Full Text
    • Lewis, M, Chang, G, Horton, NC, Kercher, MA, Pace, HC, Schumacher, MA, Brennan, RG, and Lu, P. "Crystal structure of the lactose operon represser and its complexes with DNA and inducer." Science 271, no. 5253 (1996): 1247-1254.
    • Schumacher, MA, Carter, D, Roos, DS, Ullman, B, and Brennan, RG. "Crystal structures of Toxoplasma gondii HGXPRTase reveal the catalytic role of a long flexible loop." Nature Structural Biology 3, no. 10 (1996): 881-887.  Full Text
    • Nagadoi, A, Morikawa, S, Nakamura, H, Enari, M, Kobayashi, K, Yamamoto, H, Sampei, G, Mizobuchi, K, Schumacher, MA, Brennan, RG, and Nishimura, Y. "Structural comparison of the free and DNA-bound forms of the purine repressor DNA-binding domain." Structure 3, no. 11 (1995): 1217-1224.
    • Nagadoi, A, Nakazawa, K, Morikawa, S, Nakamura, H, Sampei, G, Mizobuchi, K, Yamamoto, H, Schumacher, MA, Brennan, RG, and Nishimura, Y. "Conformational changes of purine repressor DNA-binding domain upon complexation with DNA." Nucleic acids symposium series , no. 34 (1995): 63-64.
    • Schumacher, MA, Choi, KY, Lu, F, Zalkin, H, and Brennan, RG. "Mechanism of corepressor-mediated specific DNA binding by the purine repressor." Cell 83, no. 1 (1995): 147-155.
    • Schumacher, MA, Dixon, MM, Kluger, R, Jones, RT, and Brennan, RG. "Allosteric transition intermediates modelled by crosslinked haemoglobins." Nature 375, no. 6526 (1995): 84-87.
    • Schumacher, MA, Choi, KY, Zalkin, H, and Brennan, RG. "Crystallization and preliminary X-ray analysis of an Escherichia coli purine repressor-hypoxanthine-DNA complex." Journal of Molecular Biology 242, no. 3 (1994): 302-305.  Full Text
    • Schumacher, MA, Choi, KY, Zalkin, H, and Brennan, RG. "Crystal structure of lacl member, PurR, bound to DNA: Minor groove binding by α helices." Science 266, no. 5186 (1994): 763-770.
    • Schumacher, MA, Macdonald, JR, Björkman, J, Mowbray, SL, and Brennan, RG. "Structural analysis of the purine repressor, an Escherichia coli DNA-binding protein." Journal of Biological Chemistry 268, no. 17 (1993): 12282-12288.
    • Schumacher, MA, Choi, KY, Zalkin, H, and Brennan, RG. "Crystallization and preliminary X-ray studies on the co-repressor binding domain of the Escherichia coli purine repressor." Journal of Molecular Biology 225, no. 4 (1992): 1131-1133.  Full Text
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