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Forskolin stimulation of water and cation permeability in aquaporin 1 water channels.

Publication ,  Journal Article
Yool, AJ; Stamer, WD; Regan, JW
Published in: Science
August 30, 1996

Aquaporin 1, a six-transmembrane domain protein, is a water channel present in many fluid-secreting and -absorbing cells. In Xenopus oocytes injected with aquaporin 1 complementary RNA, the application of forskolin or cyclic 8-bromo- adenosine 3',5'-monophosphate increased membrane permeability to water and triggered a cationic conductance. The cationic conductance was also induced by direct injection of protein kinase A (PKA) catalytic subunit, reduced by the kinase inhibitor H7, and blocked by HgCl2, an inhibitor of aquaporin 1. The cationic permeability of the aquaporin 1 channel is activated by a cyclic adenosine monophosphate-dependent mechanism that may involve direct or indirect phosphorylation by PKA.

Duke Scholars

Published In

Science

DOI

ISSN

0036-8075

Publication Date

August 30, 1996

Volume

273

Issue

5279

Start / End Page

1216 / 1218

Location

United States

Related Subject Headings

  • Xenopus
  • Water
  • RNA, Complementary
  • Piperazines
  • Phosphorylation
  • Patch-Clamp Techniques
  • Oocytes
  • Mercuric Chloride
  • Isoquinolines
  • Ion Channels
 

Citation

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Yool, A. J., Stamer, W. D., & Regan, J. W. (1996). Forskolin stimulation of water and cation permeability in aquaporin 1 water channels. Science, 273(5279), 1216–1218. https://doi.org/10.1126/science.273.5279.1216
Yool, A. J., W. D. Stamer, and J. W. Regan. “Forskolin stimulation of water and cation permeability in aquaporin 1 water channels.Science 273, no. 5279 (August 30, 1996): 1216–18. https://doi.org/10.1126/science.273.5279.1216.
Yool AJ, Stamer WD, Regan JW. Forskolin stimulation of water and cation permeability in aquaporin 1 water channels. Science. 1996 Aug 30;273(5279):1216–8.
Yool, A. J., et al. “Forskolin stimulation of water and cation permeability in aquaporin 1 water channels.Science, vol. 273, no. 5279, Aug. 1996, pp. 1216–18. Pubmed, doi:10.1126/science.273.5279.1216.
Yool AJ, Stamer WD, Regan JW. Forskolin stimulation of water and cation permeability in aquaporin 1 water channels. Science. 1996 Aug 30;273(5279):1216–1218.
Journal cover image

Published In

Science

DOI

ISSN

0036-8075

Publication Date

August 30, 1996

Volume

273

Issue

5279

Start / End Page

1216 / 1218

Location

United States

Related Subject Headings

  • Xenopus
  • Water
  • RNA, Complementary
  • Piperazines
  • Phosphorylation
  • Patch-Clamp Techniques
  • Oocytes
  • Mercuric Chloride
  • Isoquinolines
  • Ion Channels