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Development of a serological test for Haemophilus ducreyi for seroprevalence studies.

Publication ,  Journal Article
Elkins, C; Yi, K; Olsen, B; Thomas, C; Thomas, K; Morse, S
Published in: J Clin Microbiol
April 2000

We developed a new enzyme immunoassay (rpEIA) for use in determining the seroprevalence of chancroid. Three highly conserved outer membrane proteins from Haemophilus ducreyi strain 35000 were cloned, overexpressed, and purified from Escherichia coli for use as antigens in the rpEIA. Serum specimens from patients with and without chancroid were assayed to determine optimum sensitivity and specificity and to establish cutoff values. On the basis of these data, rpEIA was found to be both sensitive and specific when used to test a variety of serum specimens from patients with genital ulcers and urethritis and from healthy blood donors.

Duke Scholars

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

April 2000

Volume

38

Issue

4

Start / End Page

1520 / 1526

Location

United States

Related Subject Headings

  • Seroepidemiologic Studies
  • Sensitivity and Specificity
  • Recombinant Proteins
  • Microbiology
  • Immunoenzyme Techniques
  • Humans
  • Haemophilus ducreyi
  • Cloning, Molecular
  • Chancroid
  • Bacterial Outer Membrane Proteins
 

Citation

APA
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ICMJE
MLA
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Elkins, C., Yi, K., Olsen, B., Thomas, C., Thomas, K., & Morse, S. (2000). Development of a serological test for Haemophilus ducreyi for seroprevalence studies. J Clin Microbiol, 38(4), 1520–1526. https://doi.org/10.1128/JCM.38.4.1520-1526.2000
Elkins, C., K. Yi, B. Olsen, C. Thomas, K. Thomas, and S. Morse. “Development of a serological test for Haemophilus ducreyi for seroprevalence studies.J Clin Microbiol 38, no. 4 (April 2000): 1520–26. https://doi.org/10.1128/JCM.38.4.1520-1526.2000.
Elkins C, Yi K, Olsen B, Thomas C, Thomas K, Morse S. Development of a serological test for Haemophilus ducreyi for seroprevalence studies. J Clin Microbiol. 2000 Apr;38(4):1520–6.
Elkins, C., et al. “Development of a serological test for Haemophilus ducreyi for seroprevalence studies.J Clin Microbiol, vol. 38, no. 4, Apr. 2000, pp. 1520–26. Pubmed, doi:10.1128/JCM.38.4.1520-1526.2000.
Elkins C, Yi K, Olsen B, Thomas C, Thomas K, Morse S. Development of a serological test for Haemophilus ducreyi for seroprevalence studies. J Clin Microbiol. 2000 Apr;38(4):1520–1526.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

April 2000

Volume

38

Issue

4

Start / End Page

1520 / 1526

Location

United States

Related Subject Headings

  • Seroepidemiologic Studies
  • Sensitivity and Specificity
  • Recombinant Proteins
  • Microbiology
  • Immunoenzyme Techniques
  • Humans
  • Haemophilus ducreyi
  • Cloning, Molecular
  • Chancroid
  • Bacterial Outer Membrane Proteins