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Intraspinal transplantation of neurogenin-expressing stem cells generates spinal cord neural progenitors.

Publication ,  Journal Article
Lunn, JS; Pacut, C; Stern, E; Sakowski, SA; Velkey, JM; O'Shea, S; Feldman, EL
Published in: Neurobiol Dis
April 2012

Embryonic stem (ES) cells and their derivatives are an important resource for developing novel cellular therapies for disease. Controlling proliferation and lineage selection, however, are essential to circumvent the possibility of tumor formation and facilitate the safe translation of ES-based therapies to man. Expression of appropriate transcription factors is one approach to direct the differentiation of ES cells towards a specific lineage and stop proliferation. Neural differentiation can be initiated in ES cells by expression of Neurogenin1 (Ngn1). In this study we investigate the effects of controlled Ngn1 expression on mouse ES (mES) cell differentiation in vitro and following grafting into the rat spinal cord. In vitro, Ngn1 expression in mES cells leads to rapid and specific neural differentiation, and a concurrent decrease in proliferation. Similarly transplantation of Ngn1-expressing mES cells into the spinal cord lead to in situ differentiation and spinal precursor formation. These data demonstrate that Ngn1 expression in mES cells is sufficient to promote neural differentiation and inhibit proliferation, thus establishing an approach to safely graft ES cells into the spinal cord.

Duke Scholars

Published In

Neurobiol Dis

DOI

EISSN

1095-953X

Publication Date

April 2012

Volume

46

Issue

1

Start / End Page

59 / 68

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Stem Cell Transplantation
  • Spinal Cord Diseases
  • Rats, Sprague-Dawley
  • Rats
  • Neurology & Neurosurgery
  • Neurogenesis
  • Neural Stem Cells
  • Nerve Tissue Proteins
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lunn, J. S., Pacut, C., Stern, E., Sakowski, S. A., Velkey, J. M., O’Shea, S., & Feldman, E. L. (2012). Intraspinal transplantation of neurogenin-expressing stem cells generates spinal cord neural progenitors. Neurobiol Dis, 46(1), 59–68. https://doi.org/10.1016/j.nbd.2011.12.044
Lunn, J Simon, Crystal Pacut, Emily Stern, Stacey A. Sakowski, J Matthew Velkey, Sue O’Shea, and Eva L. Feldman. “Intraspinal transplantation of neurogenin-expressing stem cells generates spinal cord neural progenitors.Neurobiol Dis 46, no. 1 (April 2012): 59–68. https://doi.org/10.1016/j.nbd.2011.12.044.
Lunn JS, Pacut C, Stern E, Sakowski SA, Velkey JM, O’Shea S, et al. Intraspinal transplantation of neurogenin-expressing stem cells generates spinal cord neural progenitors. Neurobiol Dis. 2012 Apr;46(1):59–68.
Lunn, J. Simon, et al. “Intraspinal transplantation of neurogenin-expressing stem cells generates spinal cord neural progenitors.Neurobiol Dis, vol. 46, no. 1, Apr. 2012, pp. 59–68. Pubmed, doi:10.1016/j.nbd.2011.12.044.
Lunn JS, Pacut C, Stern E, Sakowski SA, Velkey JM, O’Shea S, Feldman EL. Intraspinal transplantation of neurogenin-expressing stem cells generates spinal cord neural progenitors. Neurobiol Dis. 2012 Apr;46(1):59–68.
Journal cover image

Published In

Neurobiol Dis

DOI

EISSN

1095-953X

Publication Date

April 2012

Volume

46

Issue

1

Start / End Page

59 / 68

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Stem Cell Transplantation
  • Spinal Cord Diseases
  • Rats, Sprague-Dawley
  • Rats
  • Neurology & Neurosurgery
  • Neurogenesis
  • Neural Stem Cells
  • Nerve Tissue Proteins
  • Mice