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Glutamatergic neuronal differentiation of mouse embryonic stem cells after transient expression of neurogenin 1 and treatment with BDNF and GDNF: in vitro and in vivo studies.

Publication ,  Journal Article
Reyes, JH; O'Shea, KS; Wys, NL; Velkey, JM; Prieskorn, DM; Wesolowski, K; Miller, JM; Altschuler, RA
Published in: J Neurosci
November 26, 2008

Differentiation of the pluripotent neuroepithelium into neurons and glia is accomplished by the interaction of growth factors and cell-type restricted transcription factors. One approach to obtaining a particular neuronal phenotype is by recapitulating the expression of these factors in embryonic stem (ES) cells. Toward the eventual goal of auditory nerve replacement, the aim of the current investigation was to generate auditory nerve-like glutamatergic neurons from ES cells. Transient expression of Neurog1 promoted widespread neuronal differentiation in vitro; when supplemented with brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF), 75% of ES cell-derived neurons attained a glutamatergic phenotype after 5 d in vitro. Mouse ES cells were also placed into deafened guinea pig cochleae and Neurog1 expression was induced for 48 h followed by 26 d of BDNF/GDNF infusion. In vivo differentiation resulted in 50-75% of ES cells bearing markers of early neurons, and a majority of these cells had a glutamatergic phenotype. This is the first study to report a high percentage of ES cell differentiation into a glutamatergic phenotype and sets the stage for cell replacement of auditory nerve.

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Published In

J Neurosci

DOI

EISSN

1529-2401

Publication Date

November 26, 2008

Volume

28

Issue

48

Start / End Page

12622 / 12631

Location

United States

Related Subject Headings

  • Stem Cell Transplantation
  • Sensory Receptor Cells
  • Phenotype
  • Neurology & Neurosurgery
  • Neurogenesis
  • Nerve Tissue Proteins
  • Nerve Growth Factors
  • Mice
  • Humans
  • Guinea Pigs
 

Citation

APA
Chicago
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Reyes, J. H., O’Shea, K. S., Wys, N. L., Velkey, J. M., Prieskorn, D. M., Wesolowski, K., … Altschuler, R. A. (2008). Glutamatergic neuronal differentiation of mouse embryonic stem cells after transient expression of neurogenin 1 and treatment with BDNF and GDNF: in vitro and in vivo studies. J Neurosci, 28(48), 12622–12631. https://doi.org/10.1523/JNEUROSCI.0563-08.2008
Reyes, Jeannie H., K Sue O’Shea, Noel L. Wys, J Matthew Velkey, Diane M. Prieskorn, Karolina Wesolowski, Josef M. Miller, and Richard A. Altschuler. “Glutamatergic neuronal differentiation of mouse embryonic stem cells after transient expression of neurogenin 1 and treatment with BDNF and GDNF: in vitro and in vivo studies.J Neurosci 28, no. 48 (November 26, 2008): 12622–31. https://doi.org/10.1523/JNEUROSCI.0563-08.2008.
Reyes JH, O’Shea KS, Wys NL, Velkey JM, Prieskorn DM, Wesolowski K, et al. Glutamatergic neuronal differentiation of mouse embryonic stem cells after transient expression of neurogenin 1 and treatment with BDNF and GDNF: in vitro and in vivo studies. J Neurosci. 2008 Nov 26;28(48):12622–31.
Reyes, Jeannie H., et al. “Glutamatergic neuronal differentiation of mouse embryonic stem cells after transient expression of neurogenin 1 and treatment with BDNF and GDNF: in vitro and in vivo studies.J Neurosci, vol. 28, no. 48, Nov. 2008, pp. 12622–31. Pubmed, doi:10.1523/JNEUROSCI.0563-08.2008.
Reyes JH, O’Shea KS, Wys NL, Velkey JM, Prieskorn DM, Wesolowski K, Miller JM, Altschuler RA. Glutamatergic neuronal differentiation of mouse embryonic stem cells after transient expression of neurogenin 1 and treatment with BDNF and GDNF: in vitro and in vivo studies. J Neurosci. 2008 Nov 26;28(48):12622–12631.

Published In

J Neurosci

DOI

EISSN

1529-2401

Publication Date

November 26, 2008

Volume

28

Issue

48

Start / End Page

12622 / 12631

Location

United States

Related Subject Headings

  • Stem Cell Transplantation
  • Sensory Receptor Cells
  • Phenotype
  • Neurology & Neurosurgery
  • Neurogenesis
  • Nerve Tissue Proteins
  • Nerve Growth Factors
  • Mice
  • Humans
  • Guinea Pigs