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Quantitative phase spectroscopy.

Publication ,  Journal Article
Rinehart, M; Zhu, Y; Wax, A
Published in: Biomedical optics express
May 2012

Quantitative phase spectroscopy is presented as a novel method of measuring the wavelength-dependent refractive index of microscopic volumes. Light from a broadband source is filtered to an ~5 nm bandwidth and rapidly tuned across the visible spectrum in 1 nm increments by an acousto-optic tunable filter (AOTF). Quantitative phase images of semitransparent samples are recovered at each wavelength using off-axis interferometry and are processed to recover relative and absolute dispersion measurements. We demonstrate the utility of this approach by (i) spectrally averaging phase images to reduce coherent noise, (ii) measuring absorptive and dispersive features in microspheres, and (iii) quantifying bulk hemoglobin concentrations by absolute refractive index measurements. Considerations of using low coherence illumination and the extension of spectral techniques in quantitative phase measurements are discussed.

Duke Scholars

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Published In

Biomedical optics express

DOI

EISSN

2156-7085

ISSN

2156-7085

Publication Date

May 2012

Volume

3

Issue

5

Start / End Page

958 / 965

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
  • 3212 Ophthalmology and optometry
  • 0912 Materials Engineering
  • 0205 Optical Physics
 

Citation

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Rinehart, M., Zhu, Y., & Wax, A. (2012). Quantitative phase spectroscopy. Biomedical Optics Express, 3(5), 958–965. https://doi.org/10.1364/boe.3.000958
Rinehart, Matthew, Yizheng Zhu, and Adam Wax. “Quantitative phase spectroscopy.Biomedical Optics Express 3, no. 5 (May 2012): 958–65. https://doi.org/10.1364/boe.3.000958.
Rinehart M, Zhu Y, Wax A. Quantitative phase spectroscopy. Biomedical optics express. 2012 May;3(5):958–65.
Rinehart, Matthew, et al. “Quantitative phase spectroscopy.Biomedical Optics Express, vol. 3, no. 5, May 2012, pp. 958–65. Epmc, doi:10.1364/boe.3.000958.
Rinehart M, Zhu Y, Wax A. Quantitative phase spectroscopy. Biomedical optics express. 2012 May;3(5):958–965.
Journal cover image

Published In

Biomedical optics express

DOI

EISSN

2156-7085

ISSN

2156-7085

Publication Date

May 2012

Volume

3

Issue

5

Start / End Page

958 / 965

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
  • 3212 Ophthalmology and optometry
  • 0912 Materials Engineering
  • 0205 Optical Physics