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Vascular permeability in a human tumor xenograft: molecular size dependence and cutoff size.

Publication ,  Journal Article
Yuan, F; Dellian, M; Fukumura, D; Leunig, M; Berk, DA; Torchilin, VP; Jain, RK
Published in: Cancer research
September 1995

Molecular size is one of the key determinants of transvascular transport of therapeutic agents in tumors. However, there are no data in the literature on the molecular size dependence of microvascular permeability in tumors. Therefore, we measured microvascular permeability to various macromolecules in the human colon adenocarcinoma LS174T transplanted in dorsal skin chambers in severe combined immunodeficient mice. These molecules were fluorescently labeled and injected i.v. into mice. The microvascular permeability was calculated from the fluorescence intensity measured by the intravital fluorescence microscopy technique. The value of permeability varied approximately 2-fold in the range of molecular weight from 25,000 to 160,000. These data indicate that tumor vessels are less permselective than normal vessels, presumably due to large pores in the vessel wall. The transport of macromolecules appears to be limited by diffusion through these pores. The cutoff size of the pores was estimated by observations of transvascular transport of sterically stabilized liposomes of 100-600 nm in diameter. We found that tumor vessels in our model were permeable to liposomes of up to 400 nm in diameter, suggesting that the cutoff size of the pores is between 400 and 600 nm in diameter.

Duke Scholars

Published In

Cancer research

EISSN

1538-7445

ISSN

0008-5472

Publication Date

September 1995

Volume

55

Issue

17

Start / End Page

3752 / 3756

Related Subject Headings

  • Transplantation, Heterologous
  • Ovalbumin
  • Oncology & Carcinogenesis
  • Neoplasms
  • Molecular Weight
  • Mice, SCID
  • Mice
  • Liposomes
  • Immunoglobulin G
  • Immunoglobulin Fc Fragments
 

Citation

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Yuan, F., Dellian, M., Fukumura, D., Leunig, M., Berk, D. A., Torchilin, V. P., & Jain, R. K. (1995). Vascular permeability in a human tumor xenograft: molecular size dependence and cutoff size. Cancer Research, 55(17), 3752–3756.
Yuan, F., M. Dellian, D. Fukumura, M. Leunig, D. A. Berk, V. P. Torchilin, and R. K. Jain. “Vascular permeability in a human tumor xenograft: molecular size dependence and cutoff size.Cancer Research 55, no. 17 (September 1995): 3752–56.
Yuan F, Dellian M, Fukumura D, Leunig M, Berk DA, Torchilin VP, et al. Vascular permeability in a human tumor xenograft: molecular size dependence and cutoff size. Cancer research. 1995 Sep;55(17):3752–6.
Yuan, F., et al. “Vascular permeability in a human tumor xenograft: molecular size dependence and cutoff size.Cancer Research, vol. 55, no. 17, Sept. 1995, pp. 3752–56.
Yuan F, Dellian M, Fukumura D, Leunig M, Berk DA, Torchilin VP, Jain RK. Vascular permeability in a human tumor xenograft: molecular size dependence and cutoff size. Cancer research. 1995 Sep;55(17):3752–3756.

Published In

Cancer research

EISSN

1538-7445

ISSN

0008-5472

Publication Date

September 1995

Volume

55

Issue

17

Start / End Page

3752 / 3756

Related Subject Headings

  • Transplantation, Heterologous
  • Ovalbumin
  • Oncology & Carcinogenesis
  • Neoplasms
  • Molecular Weight
  • Mice, SCID
  • Mice
  • Liposomes
  • Immunoglobulin G
  • Immunoglobulin Fc Fragments