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Radiolocalization of human mammary tumors in athymic mice by a monoclonal antibody.

Publication ,  Journal Article
Colcher, D; Zalutsky, M; Kaplan, W; Kufe, D; Austin, F; Schlom, J
Published in: Cancer Res
February 1983

Monoclonal antibody B6.2 reacts with a protein found on the surface of primary and metastatic human mammary tumors. B6.2 immunoglobulin G (IgG) was purified, F(ab')2 and Fab' fragments were generated by pepsin digestion, and the IgG and its fragments were radiolabeled with 125I; all were successful in localizing human mammary tumors transplanted into athymic mice, with tumor:tissue ratios increasing over a 4-day period. The 125I-labeled IgG gave tumor:spleen, tumor:liver, and tumor:kidney ratios of greater than 10:1 and tumor:brain and tumor:muscle ratios of 50:1 to 110:1. The F(ab')2 fragment gave higher tumor:tissue ratios than did the IgG, with tumor:liver and tumor:spleen ratios of 15:1 to 20:1. No localization of the labeled B6.2 monoclonal antibody or its fragments was observed in athymic mice bearing a human melanoma or with isotype-identical control immunoglobulin or its fragments in athymic mice bearing the mammary tumors. Imaging experiments confirmed the ability of radiolabeled monoclonal antibody B6.2 and its fragments to detect the presence of transplanted human mammary tumor lesions of less than 0.4 cm without the aid of background subtraction manipulations.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

February 1983

Volume

43

Issue

2

Start / End Page

736 / 742

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Radioimmunoassay
  • Oncology & Carcinogenesis
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice, Inbred BALB C
  • Mice
  • Immunoglobulin G
  • Immunoglobulin Fab Fragments
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Colcher, D., Zalutsky, M., Kaplan, W., Kufe, D., Austin, F., & Schlom, J. (1983). Radiolocalization of human mammary tumors in athymic mice by a monoclonal antibody. Cancer Res, 43(2), 736–742.
Colcher, D., M. Zalutsky, W. Kaplan, D. Kufe, F. Austin, and J. Schlom. “Radiolocalization of human mammary tumors in athymic mice by a monoclonal antibody.Cancer Res 43, no. 2 (February 1983): 736–42.
Colcher D, Zalutsky M, Kaplan W, Kufe D, Austin F, Schlom J. Radiolocalization of human mammary tumors in athymic mice by a monoclonal antibody. Cancer Res. 1983 Feb;43(2):736–42.
Colcher, D., et al. “Radiolocalization of human mammary tumors in athymic mice by a monoclonal antibody.Cancer Res, vol. 43, no. 2, Feb. 1983, pp. 736–42.
Colcher D, Zalutsky M, Kaplan W, Kufe D, Austin F, Schlom J. Radiolocalization of human mammary tumors in athymic mice by a monoclonal antibody. Cancer Res. 1983 Feb;43(2):736–742.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

February 1983

Volume

43

Issue

2

Start / End Page

736 / 742

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Radioimmunoassay
  • Oncology & Carcinogenesis
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice, Inbred BALB C
  • Mice
  • Immunoglobulin G
  • Immunoglobulin Fab Fragments
  • Humans