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Id proteins synchronize stemness and anchorage to the niche of neural stem cells.

Publication ,  Journal Article
Niola, F; Zhao, X; Singh, D; Castano, A; Sullivan, R; Lauria, M; Nam, H-S; Zhuang, Y; Benezra, R; Di Bernardo, D; Iavarone, A; Lasorella, A
Published in: Nat Cell Biol
April 22, 2012

Stem-cell functions require activation of stem-cell-intrinsic transcriptional programs and extracellular interaction with a niche microenvironment. How the transcriptional machinery controls residency of stem cells in the niche is unknown. Here we show that Id proteins coordinate stem-cell activities with anchorage of neural stem cells (NSCs) to the niche. Conditional inactivation of three Id genes in NSCs triggered detachment of embryonic and postnatal NSCs from the ventricular and vascular niche, respectively. The interrogation of the gene modules directly targeted by Id deletion in NSCs revealed that Id proteins repress bHLH-mediated activation of Rap1GAP, thus serving to maintain the GTPase activity of RAP1, a key mediator of cell adhesion. Preventing the elevation of the Rap1GAP level countered the consequences of Id loss on NSC-niche interaction and stem-cell identity. Thus, by preserving anchorage of NSCs to the extracellular environment, Id activity synchronizes NSC functions to residency in the specialized niche.

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Published In

Nat Cell Biol

DOI

EISSN

1476-4679

Publication Date

April 22, 2012

Volume

14

Issue

5

Start / End Page

477 / 487

Location

England

Related Subject Headings

  • Neural Stem Cells
  • Mice
  • GTPase-Activating Proteins
  • Developmental Biology
  • Cell Adhesion
  • Basic Helix-Loop-Helix Transcription Factors
  • Antigens, Neoplasm
  • Animals
  • 3101 Biochemistry and cell biology
  • 11 Medical and Health Sciences
 

Citation

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Niola, F., Zhao, X., Singh, D., Castano, A., Sullivan, R., Lauria, M., … Lasorella, A. (2012). Id proteins synchronize stemness and anchorage to the niche of neural stem cells. Nat Cell Biol, 14(5), 477–487. https://doi.org/10.1038/ncb2490
Niola, Francesco, Xudong Zhao, Devendra Singh, Angelica Castano, Ryan Sullivan, Mario Lauria, Hyung-song Nam, et al. “Id proteins synchronize stemness and anchorage to the niche of neural stem cells.Nat Cell Biol 14, no. 5 (April 22, 2012): 477–87. https://doi.org/10.1038/ncb2490.
Niola F, Zhao X, Singh D, Castano A, Sullivan R, Lauria M, et al. Id proteins synchronize stemness and anchorage to the niche of neural stem cells. Nat Cell Biol. 2012 Apr 22;14(5):477–87.
Niola, Francesco, et al. “Id proteins synchronize stemness and anchorage to the niche of neural stem cells.Nat Cell Biol, vol. 14, no. 5, Apr. 2012, pp. 477–87. Pubmed, doi:10.1038/ncb2490.
Niola F, Zhao X, Singh D, Castano A, Sullivan R, Lauria M, Nam H-S, Zhuang Y, Benezra R, Di Bernardo D, Iavarone A, Lasorella A. Id proteins synchronize stemness and anchorage to the niche of neural stem cells. Nat Cell Biol. 2012 Apr 22;14(5):477–487.

Published In

Nat Cell Biol

DOI

EISSN

1476-4679

Publication Date

April 22, 2012

Volume

14

Issue

5

Start / End Page

477 / 487

Location

England

Related Subject Headings

  • Neural Stem Cells
  • Mice
  • GTPase-Activating Proteins
  • Developmental Biology
  • Cell Adhesion
  • Basic Helix-Loop-Helix Transcription Factors
  • Antigens, Neoplasm
  • Animals
  • 3101 Biochemistry and cell biology
  • 11 Medical and Health Sciences