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Beta-catenin mediates soft tissue contracture in clubfoot.

Publication ,  Journal Article
Poon, R; Li, C; Alman, BA
Published in: Clin Orthop Relat Res
May 2009

The contracted tissues from clubfeet resemble tissues from other fibroproliferative disorders such as palmar fibromatosis. Beta-catenin-mediated signaling is a crucial pathway controlling the fibroproliferative response in many fibroproliferative disorders. To determine if beta-catenin signaling plays a role in clubfoot, contracted and less contracted tissues from clubfeet were studied using Western analysis to determine the protein level of beta-catenin. Primary cell cultures were established from these tissues, and they were treated with either lithium to increase beta-catenin or Dickkopf-1 to inhibit beta-catenin. RNA was extracted from the cells and analyzed to determine how beta-catenin regulates expression of Type III collagen, an extracellular matrix protein upregulated in contracted clubfoot tissue. There was a more than twofold increase in beta-catenin protein in the contracted tissues. Treatment with either lithium or Dickkopf-1 showed Type III collagen RNA expression positively correlated with the protein level of beta-catenin. These data support the concept that beta-catenin-mediated signaling plays an important role regulating contracture in clubfeet. Because pharmacologic agents are under development to block this signaling pathway, such drugs could be used in cases of severe stiffness to improve range of motion or to decrease the need for radical surgical approaches.

Duke Scholars

Published In

Clin Orthop Relat Res

DOI

EISSN

1528-1132

Publication Date

May 2009

Volume

467

Issue

5

Start / End Page

1180 / 1185

Location

United States

Related Subject Headings

  • beta Catenin
  • Up-Regulation
  • Signal Transduction
  • RNA
  • Phosphorylation
  • Orthopedics
  • Lithium Chloride
  • Humans
  • Glycogen Synthase Kinase 3 beta
  • Glycogen Synthase Kinase 3
 

Citation

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Poon, R., Li, C., & Alman, B. A. (2009). Beta-catenin mediates soft tissue contracture in clubfoot. Clin Orthop Relat Res, 467(5), 1180–1185. https://doi.org/10.1007/s11999-008-0692-7
Poon, Raymond, Catherine Li, and Benjamin A. Alman. “Beta-catenin mediates soft tissue contracture in clubfoot.Clin Orthop Relat Res 467, no. 5 (May 2009): 1180–85. https://doi.org/10.1007/s11999-008-0692-7.
Poon R, Li C, Alman BA. Beta-catenin mediates soft tissue contracture in clubfoot. Clin Orthop Relat Res. 2009 May;467(5):1180–5.
Poon, Raymond, et al. “Beta-catenin mediates soft tissue contracture in clubfoot.Clin Orthop Relat Res, vol. 467, no. 5, May 2009, pp. 1180–85. Pubmed, doi:10.1007/s11999-008-0692-7.
Poon R, Li C, Alman BA. Beta-catenin mediates soft tissue contracture in clubfoot. Clin Orthop Relat Res. 2009 May;467(5):1180–1185.
Journal cover image

Published In

Clin Orthop Relat Res

DOI

EISSN

1528-1132

Publication Date

May 2009

Volume

467

Issue

5

Start / End Page

1180 / 1185

Location

United States

Related Subject Headings

  • beta Catenin
  • Up-Regulation
  • Signal Transduction
  • RNA
  • Phosphorylation
  • Orthopedics
  • Lithium Chloride
  • Humans
  • Glycogen Synthase Kinase 3 beta
  • Glycogen Synthase Kinase 3