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Genetic interaction screens identify a role for hedgehog signaling in Drosophila border cell migration.

Publication ,  Journal Article
Geisbrecht, ER; Sawant, K; Su, Y; Liu, ZC; Silver, DL; Burtscher, A; Wang, X; Zhu, AJ; McDonald, JA
Published in: Dev Dyn
May 2013

BACKGROUND: Cell motility is essential for embryonic development and physiological processes such as the immune response, but also contributes to pathological conditions such as tumor progression and inflammation. However, our understanding of the mechanisms underlying migratory processes is incomplete. Drosophila border cells provide a powerful genetic model to identify the roles of genes that contribute to cell migration. RESULTS: Members of the Hedgehog signaling pathway were uncovered in two independent screens for interactions with the small GTPase Rac and the polarity protein Par-1 in border cell migration. Consistent with a role in migration, multiple Hh signaling components were enriched in the migratory border cells. Interference with Hh signaling by several different methods resulted in incomplete cell migration. Moreover, the polarized distribution of E-Cadherin and a marker of tyrosine kinase activity were altered when Hh signaling was disrupted. Conservation of Hh-Rac and Hh-Par-1 signaling was illustrated in the wing, in which Hh-dependent phenotypes were enhanced by loss of Rac or par-1. CONCLUSIONS: We identified a pathway by which Hh signaling connects to Rac and Par-1 in cell migration. These results further highlight the importance of modifier screens in the identification of new genes that function in developmental pathways.

Duke Scholars

Published In

Dev Dyn

DOI

EISSN

1097-0177

Publication Date

May 2013

Volume

242

Issue

5

Start / End Page

414 / 431

Location

United States

Related Subject Headings

  • Signal Transduction
  • Ovary
  • Oogenesis
  • Morphogenesis
  • Male
  • Hedgehog Proteins
  • Gene Regulatory Networks
  • Gene Expression Regulation, Developmental
  • Gene Expression Profiling
  • Female
 

Citation

APA
Chicago
ICMJE
MLA
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Geisbrecht, E. R., Sawant, K., Su, Y., Liu, Z. C., Silver, D. L., Burtscher, A., … McDonald, J. A. (2013). Genetic interaction screens identify a role for hedgehog signaling in Drosophila border cell migration. Dev Dyn, 242(5), 414–431. https://doi.org/10.1002/dvdy.23926
Geisbrecht, Erika R., Ketki Sawant, Ying Su, Ze Cindy Liu, Debra L. Silver, Ashley Burtscher, Xuejiao Wang, Alan Jian Zhu, and Jocelyn A. McDonald. “Genetic interaction screens identify a role for hedgehog signaling in Drosophila border cell migration.Dev Dyn 242, no. 5 (May 2013): 414–31. https://doi.org/10.1002/dvdy.23926.
Geisbrecht ER, Sawant K, Su Y, Liu ZC, Silver DL, Burtscher A, et al. Genetic interaction screens identify a role for hedgehog signaling in Drosophila border cell migration. Dev Dyn. 2013 May;242(5):414–31.
Geisbrecht, Erika R., et al. “Genetic interaction screens identify a role for hedgehog signaling in Drosophila border cell migration.Dev Dyn, vol. 242, no. 5, May 2013, pp. 414–31. Pubmed, doi:10.1002/dvdy.23926.
Geisbrecht ER, Sawant K, Su Y, Liu ZC, Silver DL, Burtscher A, Wang X, Zhu AJ, McDonald JA. Genetic interaction screens identify a role for hedgehog signaling in Drosophila border cell migration. Dev Dyn. 2013 May;242(5):414–431.
Journal cover image

Published In

Dev Dyn

DOI

EISSN

1097-0177

Publication Date

May 2013

Volume

242

Issue

5

Start / End Page

414 / 431

Location

United States

Related Subject Headings

  • Signal Transduction
  • Ovary
  • Oogenesis
  • Morphogenesis
  • Male
  • Hedgehog Proteins
  • Gene Regulatory Networks
  • Gene Expression Regulation, Developmental
  • Gene Expression Profiling
  • Female