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Cigarette smoke-induced placental adrenomedullin expression and trophoblast cell invasion.

Publication ,  Journal Article
Kraus, DM; Feng, L; Heine, RP; Brown, HL; Caron, KM; Murtha, AP; Grotegut, CA
Published in: Reprod Sci
January 2014

Smoking in pregnancy reduces preeclampsia risk, but the mechanism of this effect is unknown. Prior studies have demonstrated that women with preeclampsia have lower placental adrenomedullin (AM) expression, and cigarette smoke extract (CSE) treatment of placental trophoblast cells in culture increases AM cellular production. We hypothesized that CSE alters trophoblast invasion through an AM-mediated mechanism, and that placental AM expression is greater among smokers. HTR-8/SVneo trophoblast cells were incubated for 24 hours in Matrigel-invasion chambers with 6 treatment groups: nonstimulated (NS), AM, AM inhibitor (AM22-52), 1% CSE, AM + AM22-52, and 1% CSE + AM22-52. Cells that penetrated the lower surface of the chambers were quantified, invasion indices were calculated, and compared using a 1-way analysis of variance with Bonferroni corrections for multiple comparisons. Trophoblast cells treated with both AM and 1% CSE demonstrated increased cellular invasion compared to NS controls (1.5-fold [P < .01] and 1.45-fold [P < .01], respectively). Cotreatment with the AM inhibitor significantly attenuated the increased invasion seen with both AM and CSE alone. Next, the placental tissue was obtained from 11 smokers and 11 nonsmokers at term and processed for immunohistochemistry (IHC) and real-time quantitative polymerase chain reaction (PCR) for AM. Placentas from smokers demonstrated more intense AM staining and increased AM gene (ADM) expression compared to placentas from nonsmokers (P = .004 for IHC, P = .022 for PCR). The CSE increases trophoblast cell invasion through an AM-mediated process, and placental AM expression is increased among term smokers compared to nonsmokers. These findings provide evidence that the AM pathway may play a role in the protection from preeclampsia seen in smokers.

Duke Scholars

Published In

Reprod Sci

DOI

EISSN

1933-7205

Publication Date

January 2014

Volume

21

Issue

1

Start / End Page

63 / 71

Location

United States

Related Subject Headings

  • Young Adult
  • Trophoblasts
  • Smoking
  • Smoke
  • Signal Transduction
  • RNA, Messenger
  • Pregnancy
  • Pre-Eclampsia
  • Obstetrics & Reproductive Medicine
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
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Kraus, D. M., Feng, L., Heine, R. P., Brown, H. L., Caron, K. M., Murtha, A. P., & Grotegut, C. A. (2014). Cigarette smoke-induced placental adrenomedullin expression and trophoblast cell invasion. Reprod Sci, 21(1), 63–71. https://doi.org/10.1177/1933719113488456
Kraus, Daniel M., Liping Feng, R Phillips Heine, Haywood L. Brown, Kathleen M. Caron, Amy P. Murtha, and Chad A. Grotegut. “Cigarette smoke-induced placental adrenomedullin expression and trophoblast cell invasion.Reprod Sci 21, no. 1 (January 2014): 63–71. https://doi.org/10.1177/1933719113488456.
Kraus DM, Feng L, Heine RP, Brown HL, Caron KM, Murtha AP, et al. Cigarette smoke-induced placental adrenomedullin expression and trophoblast cell invasion. Reprod Sci. 2014 Jan;21(1):63–71.
Kraus, Daniel M., et al. “Cigarette smoke-induced placental adrenomedullin expression and trophoblast cell invasion.Reprod Sci, vol. 21, no. 1, Jan. 2014, pp. 63–71. Pubmed, doi:10.1177/1933719113488456.
Kraus DM, Feng L, Heine RP, Brown HL, Caron KM, Murtha AP, Grotegut CA. Cigarette smoke-induced placental adrenomedullin expression and trophoblast cell invasion. Reprod Sci. 2014 Jan;21(1):63–71.
Journal cover image

Published In

Reprod Sci

DOI

EISSN

1933-7205

Publication Date

January 2014

Volume

21

Issue

1

Start / End Page

63 / 71

Location

United States

Related Subject Headings

  • Young Adult
  • Trophoblasts
  • Smoking
  • Smoke
  • Signal Transduction
  • RNA, Messenger
  • Pregnancy
  • Pre-Eclampsia
  • Obstetrics & Reproductive Medicine
  • Humans