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Nicotinamide pre-treatment ameliorates NAD(H) hyperoxidation and improves neuronal function after severe hypoxia.

Publication ,  Journal Article
Shetty, PK; Galeffi, F; Turner, DA
Published in: Neurobiol Dis
February 2014

Prolonged hypoxia leads to irreversible loss of neuronal function and metabolic impairment of nicotinamide adenine dinucleotide recycling (between NAD(+) and NADH) immediately after reoxygenation, resulting in NADH hyperoxidation. We test whether the addition of nicotinamide (to enhance NAD(+) levels) or PARP-1 inhibition (to prevent consumption of NAD(+)) can be effective in improving either loss of neuronal function or hyperoxidation following severe hypoxic injury in hippocampal slices. After severe, prolonged hypoxia (maintained for 3min after spreading depression) there was hyperoxidation of NADH following reoxygenation, an increased soluble NAD(+)/NADH ratio, loss of neuronal field excitatory post-synaptic potential (fEPSP) and decreased ATP content. Nicotinamide incubation (5mM) 2h prior to hypoxia significantly increased total NAD(H) content, improved neuronal recovery, enhanced ATP content, and prevented NADH hyperoxidation. The nicotinamide-induced increase in total soluble NAD(H) was more significant in the cytosolic compartment than within mitochondria. Prolonged incubation with PJ-34 (>1h) led to enhanced baseline NADH fluorescence prior to hypoxia, as well as improved neuronal recovery, NADH hyperoxidation and ATP content on recovery from severe hypoxia and reoxygenation. In this acute model of severe neuronal dysfunction prolonged incubation with either nicotinamide or PJ-34 prior to hypoxia improved recovery of neuronal function, enhanced NADH reduction and ATP content, but neither treatment restored function when administered during or after prolonged hypoxia and reoxygenation.

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Published In

Neurobiol Dis

DOI

EISSN

1095-953X

Publication Date

February 2014

Volume

62

Start / End Page

469 / 478

Location

United States

Related Subject Headings

  • Rats, Inbred F344
  • Rats
  • Niacinamide
  • Neuroprotective Agents
  • Neurons
  • Neurology & Neurosurgery
  • NAD
  • Mitochondria
  • Male
  • In Vitro Techniques
 

Citation

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Shetty, P. K., Galeffi, F., & Turner, D. A. (2014). Nicotinamide pre-treatment ameliorates NAD(H) hyperoxidation and improves neuronal function after severe hypoxia. Neurobiol Dis, 62, 469–478. https://doi.org/10.1016/j.nbd.2013.10.025
Shetty, Pavan K., Francesca Galeffi, and Dennis A. Turner. “Nicotinamide pre-treatment ameliorates NAD(H) hyperoxidation and improves neuronal function after severe hypoxia.Neurobiol Dis 62 (February 2014): 469–78. https://doi.org/10.1016/j.nbd.2013.10.025.
Shetty, Pavan K., et al. “Nicotinamide pre-treatment ameliorates NAD(H) hyperoxidation and improves neuronal function after severe hypoxia.Neurobiol Dis, vol. 62, Feb. 2014, pp. 469–78. Pubmed, doi:10.1016/j.nbd.2013.10.025.
Journal cover image

Published In

Neurobiol Dis

DOI

EISSN

1095-953X

Publication Date

February 2014

Volume

62

Start / End Page

469 / 478

Location

United States

Related Subject Headings

  • Rats, Inbred F344
  • Rats
  • Niacinamide
  • Neuroprotective Agents
  • Neurons
  • Neurology & Neurosurgery
  • NAD
  • Mitochondria
  • Male
  • In Vitro Techniques