Gustavo M. Silva
Assistant Professor of Biology
My main research goal is to understand and be able to control how cells respond to stressful and harmful conditions, which are the underlying causes of many human diseases. To achieve this goal, I study cellular response to stress at the protein level and aim to characterize the different regulatory functions mediated by the ubiquitin-proteasome system (UPS), essential machinery involved in modulating protein dynamics. Ultimately, regulating specific UPS roles will provide new tools to increase cellular tolerance to a variety of environmental stresses, which is highly relevant for a variety of degenerative diseases. The main focus of my lab is to investigate the unprecedented regulation of translation mediated by ubiquitin. I laid the groundwork for this research investigating the ubiquitination response in the budding yeast Saccharomyces cerevisiae
and we will explore the evolutionary conservation of this pathway and its function in neuronal cells. Our lab is excited to keep pushing the field forward and to use a combination of proteomics, genomics, and molecular methods to understand the mechanisms by which ubiquitin regulates translation, and ultimately, cellular response to stress.
Current Appointments & Affiliations
- Assistant Professor of Biology, Biology, Trinity College of Arts & Sciences 2017
- Assistant Professor of Cell Biology, Cell Biology, Basic Science Departments 2022
Contact Information
- 130 Science Drive, 3103 French, Durham, NC 27708
- 130 Science Drive, Box 90338, Durham, NC 27708
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gustavo.silva@duke.edu
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Silva lab at Duke University
- Background
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Education, Training, & Certifications
- Associate Research Scientist, Center For Genomics And Systems Biology, Department Of Biology, New York University 2011 - 2017
- Ph.D., University of Sao Paulo (Brazil) 2010
- Lic., University of Sao Paulo (Brazil) 2007
- B.Sc., University of Sao Paulo (Brazil) 2004
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Academic Positions Outside Duke
- Proteomics Facility Manager, New York University. 2014 - 2017
- Recognition
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In the News
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APR 6, 2023 Duke Today -
OCT 19, 2022 -
MAY 27, 2022 -
MAR 29, 2022 -
MAY 12, 2021 -
MAY 9, 2019 -
AUG 8, 2018 -
NOV 30, 2017 -
NOV 6, 2017 -
FEB 11, 2015 Agencia FAPESP -
FEB 5, 2015 Redoxoma -
JAN 26, 2015 NYU press -
MAY 16, 2012 Agencia FAPESP
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Awards & Honors
- Faculty of 1000 Research Prize. NYU/F1000. 2015
- Travel award for VI Meeting. Society for Free Radical in Biology and Medicine. 2009
- Travel award for V Meeting. Society for Free Radical in Biology and Medicine. 2007
- Young Investigator Award. Butantan Institute. 2006
- SBBq Award. Brazilian Society of Biochemistry and Molecular Biology. 2005
- Research
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Selected Grants
- Duke Preparing Research Scholars in Biomedical Sciences- Post-Baccalaureate Research Education Program awarded by National Institutes of Health 2022 - 2027
- Medical Scientist Training Program awarded by National Institutes of Health 2022 - 2027
- Cell and Molecular Biology Training Program awarded by National Institutes of Health 2021 - 2026
- Regulation of RIG-I signaling and viral immune evasion by ufmylation awarded by National Institutes of Health 2021 - 2026
- Defining Novel Roles of Ubiquitin Accumulation During the Mammalian Oxidative Stress Response awarded by National Institutes of Health 2022 - 2025
- Dissecting the roles of ubiquitin in translation control awarded by National Institutes of Health 2020 - 2025
- Genetic and Genomics Training Grant awarded by National Institutes of Health 2020 - 2025
- A Hybrid Computational Platform for High-resolution Structure Determination Using Cryo-EM awarded by National Institutes of Health 2021 - 2025
- Characterizing new redox roles for protein ubiquitination in human cells awarded by National Institutes of Health 2022 - 2024
- Defining Novel Roles of Ubiquitin Accumulation During the Mammalian Oxidative Stress Response awarded by Burroughs Wellcome Fund 2021 - 2023
- Medical Scientist Training Program awarded by National Institutes of Health 1997 - 2022
- Defining the roles of ubiquitination during the environmental stress response awarded by National Institutes of Health 2018 - 2022
- Genetics Training Grant awarded by National Institutes of Health 1979 - 2020
- Organization and Function of Cellular Structure awarded by National Institutes of Health 1975 - 2020
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Fellowships, Supported Research, & Other Grants
- Seed Grant: The Black Think Tank awarded by Duke Office of Faculty Advancement 2019
- - Linkage-specific ubiquitylation patterns as highly sensitive markers for neurodegenerative diseases awarded by NIH (SBIR) 2016 - 2018
- Defining the roles of ubiquitination during the environmental stress response awarded by NIH (K99) 2015 - 2017
- Modeling Protein Degradation - Evaluation of Strategies and Targets awarded by NSF (EAGER) 2013 - 2015
- Characterization of the S-glutathiolation of the yeast 20S Proteasome awarded by PhD FAPESP Fellowship 2005 - 2010
- Study of 20S Proteasome redox regulation awarded by CNPq Undergraduate Fellowship 2001 - 2004
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External Relationships
- American Society for Biochemistry and Molecular Biology
- Publications & Artistic Works
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Selected Publications
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Academic Articles
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Simões, Vanessa, Blanche K. Cizubu, Lana Harley, Ye Zhou, Joshua Pajak, Nathan A. Snyder, Jonathan Bouvette, et al. “Redox-sensitive E2 Rad6 controls cellular response to oxidative stress via K63-linked ubiquitination of ribosomes.” Cell Rep 39, no. 8 (May 24, 2022): 110860. https://doi.org/10.1016/j.celrep.2022.110860.Full Text Link to Item
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Snyder, Nathan A., and Gustavo M. Silva. “Deubiquitinating enzymes (DUBs): Regulation, homeostasis, and oxidative stress response.” Journal of Biological Chemistry 297, no. 3 (September 2021): 101077–101077. https://doi.org/10.1016/j.jbc.2021.101077.Full Text
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Zhou, Ye, Panagiotis L. Kastritis, Shannon E. Dougherty, Jonathan Bouvette, Allen L. Hsu, Laura Burbaum, Shyamal Mosalaganti, et al. “Structural impact of K63 ubiquitin on yeast translocating ribosomes under oxidative stress.” Proc Natl Acad Sci U S A 117, no. 36 (September 8, 2020): 22157–66. https://doi.org/10.1073/pnas.2005301117.Full Text Link to Item
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Blount, Jessica R., Kozeta Libohova, Gustavo M. Silva, and Sokol V. Todi. “Isoleucine 44 Hydrophobic Patch Controls Toxicity of Unanchored, Linear Ubiquitin Chains through NF-κB Signaling.” Cells 9, no. 6 (June 2020): E1519. https://doi.org/10.3390/cells9061519.Full Text
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Dougherty, Shannon E., Austin O. Maduka, Toshifumi Inada, and Gustavo M. Silva. “Expanding Role of Ubiquitin in Translational Control.” International Journal of Molecular Sciences 21, no. 3 (February 9, 2020): 1151–1151. https://doi.org/10.3390/ijms21031151.Full Text
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Manohar, Sandhya, Samson Jacob, Jade Wang, Keira A. Wiechecki, Hiromi W. L. Koh, Vanessa Simões, Hyungwon Choi, Christine Vogel, and Gustavo M. Silva. “Polyubiquitin Chains Linked by Lysine Residue 48 (K48) Selectively Target Oxidized Proteins In Vivo.” Antioxidants & Redox Signaling 31, no. 15 (November 2019): 1133–49. https://doi.org/10.1089/ars.2019.7826.Full Text
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Back, Songhee, Andrew W. Gorman, Christine Vogel, and Gustavo M. Silva. “Site-Specific K63 Ubiquitinomics Provides Insights into Translation Regulation under Stress.” Journal of Proteome Research 18, no. 1 (January 2019): 309–18. https://doi.org/10.1021/acs.jproteome.8b00623.Full Text
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Lin, Yu-Cheng, Matthew D. Sekedat, William Cole Cornell, Gustavo M. Silva, Chinweike Okegbe, Alexa Price-Whelan, Christine Vogel, and Lars E. P. Dietrich. “Phenazines Regulate Nap-Dependent Denitrification in Pseudomonas aeruginosa Biofilms.” Edited by Conrad W. Mullineaux. Journal of Bacteriology 200, no. 9 (May 2018). https://doi.org/10.1128/jb.00031-18.Full Text
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Silva, Gustavo Monteiro, and Christine Vogel. “Quantifying gene expression: the importance of being subtle.” Molecular Systems Biology 12, no. 10 (October 2016). https://doi.org/10.15252/msb.20167325.Full Text
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Costa, João Pinto da, Rui Vitorino, Gustavo M. Silva, Christine Vogel, Armando C. Duarte, and Teresa Rocha-Santos. “A synopsis on aging—Theories, mechanisms and future prospects.” Ageing Research Reviews 29 (August 2016): 90–112. https://doi.org/10.1016/j.arr.2016.06.005.Full Text
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Toledo, Rodrigo A., Yuejuan Qin, Zi-Ming Cheng, Qing Gao, Shintaro Iwata, Gustavo M. Silva, Manju L. Prasad, et al. “Recurrent Mutations of Chromatin-Remodeling Genes and Kinase Receptors in Pheochromocytomas and Paragangliomas.” Clinical Cancer Research 22, no. 9 (May 1, 2016): 2301–10. https://doi.org/10.1158/1078-0432.ccr-15-1841.Full Text
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Silva, G. M., W. Wei, S. Manohar, and C. Vogel. “Identification and quantification of K63-ubiquitinated proteins in neuronal cells by high-resolution mass spectrometry.” Neuromethods 114 (November 18, 2015). https://doi.org/10.1007/7657_2015_95.Full Text Link to Item
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Silva, Gustavo Monteiro, and Christine Vogel. “Mass spectrometry analysis of K63-ubiquitinated targets in response to oxidative stress.” Data in Brief 4 (September 2015): 130–34. https://doi.org/10.1016/j.dib.2015.05.002.Full Text
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Silva, Gustavo M., Daniel Finley, and Christine Vogel. “K63 polyubiquitination is a new modulator of the oxidative stress response.” Nature Structural & Molecular Biology 22, no. 2 (February 2015): 116–23. https://doi.org/10.1038/nsmb.2955.Full Text
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Tchourine, Konstantine, Christopher S. Poultney, Li Wang, Gustavo M. Silva, Sandhya Manohar, Christian L. Mueller, Richard Bonneau, and Christine Vogel. “One third of dynamic protein expression profiles can be predicted by a simple rate equation.” Mol. Biosyst. 10, no. 11 (August 1, 2014): 2850–62. https://doi.org/10.1039/c4mb00358f.Full Text
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Demasi, Marilene, Luis E. S. Netto, Gustavo M. Silva, Adrian Hand, Cristiano L. P. de Oliveira, Renata N. Bicev, Fabio Gozzo, Mario H. Barros, Janaina M. M. Leme, and Erina Ohara. “Redox regulation of the proteasome via S-glutathionylation.” Redox Biology 2 (2014): 44–51. https://doi.org/10.1016/j.redox.2013.12.003.Full Text
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Malvezzi, Alberto, Patrícia M. Higa, Antonia T-do Amaral, Gustavo M. Silva, Fabio C. Gozzo, Emer S. Ferro, Leandro M. Castro, Leandro de Rezende, Gisele Monteiro, and Marilene Demasi. “The Cysteine-Rich Protein Thimet Oligopeptidase as a Model of the Structural Requirements for S-glutathiolation and Oxidative Oligomerization.” Edited by Peter Csermely. Plos One 7, no. 6 (June 25, 2012): e39408–e39408. https://doi.org/10.1371/journal.pone.0039408.Full Text
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Silva, Gustavo M., Luis E. S. Netto, Vanessa Simões, Luiz F. A. Santos, Fabio C. Gozzo, Marcos A. A. Demasi, Cristiano L. P. Oliveira, et al. “Redox Control of 20S Proteasome Gating.” Antioxidants &Amp; Redox Signaling 16, no. 11 (June 2012): 1183–94. https://doi.org/10.1089/ars.2011.4210.Full Text
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Vogel, Christine, Gustavo Monteiro Silva, and Edward M. Marcotte. “Protein Expression Regulation under Oxidative Stress.” Molecular &Amp; Cellular Proteomics 10, no. 12 (December 2011): M111.009217-M111.009217. https://doi.org/10.1074/mcp.m111.009217.Full Text
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Silva, Gustavo M., Luis E. S. Netto, Karen F. Discola, Gilberto M. Piassa-Filho, Daniel C. Pimenta, José A. Bárcena, and Marilene Demasi. “Role of glutaredoxin 2 and cytosolic thioredoxins in cysteinyl-based redox modification of the 20S proteasome.” Febs Journal 275, no. 11 (June 2008): 2942–55. https://doi.org/10.1111/j.1742-4658.2008.06441.x.Full Text
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Netto, Luis Eduardo Soares, Marcos Antonio de Oliveira, Gisele Monteiro, Ana Paula Dias Demasi, José Renato Rosa Cussiol, Karen Fulan Discola, Marilene Demasi, et al. “Reactive cysteine in proteins: Protein folding, antioxidant defense, redox signaling and more.” Comparative Biochemistry and Physiology Part C: Toxicology &Amp; Pharmacology 146, no. 1–2 (July 2007): 180–93. https://doi.org/10.1016/j.cbpc.2006.07.014.Full Text
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Discola, Karen Fulan, Marcos Antonio de Oliveira, Gustavo Monteiro Silva, José Antonio Barcena, Pablo Porras, Alicia Padilla, Luis Eduardo Soares Netto, and Beatriz Gomes Guimarães. “Crystallization and preliminary X-ray crystallographic studies of glutaredoxin 2 from Saccharomyces cerevisiae in different oxidation states.” Acta Crystallographica. Section F, Structural Biology and Crystallization Communications 61, no. Pt 4 (April 2005): 445–47. https://doi.org/10.1107/s1744309105008730.Full Text
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Demasi, Marilene, Gustavo Monteiro Silva, and Luis Eduardo Soares Netto. “20 S Proteasome from Saccharomyces cerevisiae Is Responsive to Redox Modifications and IsS-Glutathionylated.” Journal of Biological Chemistry 278, no. 1 (January 2003): 679–85. https://doi.org/10.1074/jbc.m209282200.Full Text
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Wiechecki, Keira, Sandhya Manohar, Gustavo Silva, Konstantine Tchourine, Samson Jacob, Angelo Valleriani, and Christine Vogel. “Integrative Meta-Analysis Reveals That Most Yeast Proteins Are Very Stable (Accepted).” Ssrn Electronic Journal, n.d. https://doi.org/10.2139/ssrn.3155916.Full Text
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Book Sections
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Silva, G. M., W. Wei, S. Manohar, and C. Vogel. “Identification and quantification of K63-ubiquitinated proteins in neuronal cells by high-resolution mass spectrometry.” In Analysis of Post-Translational Modifications and Proteolysis in Neuroscience, 114:111–25. New York, NY: Humana Press, 2016. https://doi.org/10.1007/7657_2015_95.Full Text
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- Teaching & Mentoring
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Recent Courses
- BIOLOGY 493: Research Independent Study 2023
- BIOLOGY 525S: Proteomics Methods in Life Sciences 2023
- BIOLOGY 791T: Tutorial 2023
- CMB 525S: Proteomics Methods in Life Sciences 2023
- BIOLOGY 201L: Gateway to Biology: Molecular Biology 2022
- BIOLOGY 293: Research Independent Study 2022
- BIOLOGY 493: Research Independent Study 2022
- BIOLOGY 791T: Tutorial 2022
- UPGEN 716S: Genetics Student Research 2022
- BIOLOGY 201: Gateway to Biology: Molecular Biology 2021
- BIOLOGY 201L: Gateway to Biology: Molecular Biology 2021
- BIOLOGY 293: Research Independent Study 2021
- BIOLOGY 792: Research 2021
- UPGEN 716S: Genetics Student Research 2021
- Scholarly, Clinical, & Service Activities
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Outreach & Engaged Scholarship
- Assistant Coordinator. Summer Undergraduate Research Program for Diversity. New York University. June 2017 - August 2017 2017
- Assistant Coordinator. Summer Undergraduate Research Program for Diversity. New York University. June 2016 - August 2016 2016
- Mentor for High School Students. Applied Research Innovations in Science and Engineering (ARISE). New York University. July 2015 - August 2015 2015
- Host. Open Day of Center for Talented Youth (CTY-NYU). New York University. 2015 - 2015 2015
- Mentor for High School Student. Applied Research Innovations in Science and Engineering (ARISE). New York University. July 2014 - August 2014 2014
- Host. Open Day of Center for Talented Youth (CTY-NYU). New York University. 2014 - 2014 2014
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Service to the Profession
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