David Needham
Professor in the Department of Mechanical Engineering and Materials Science
Professor Needham has been at Duke since 1987 and over the years has developed many collaborative and scholarly relationships across the campus and Medical School. He holds Faculty and membership appointments as: Associate Professor of Biomedical Engineering; Center for Bioinspired Materials and Material Systems; Center for Biomolecular and Tissue Engineering; Duke Comprehensive Cancer Center; and the Duke Cancer Institute. Internationally, he holds a joint appointment as Professor of Translational Therapeutics in the School of Pharmacy, at the University of Nottingham, UK. He also collaborates with preclinical researchers at the Erasmus University Medical Center, in Rotterdam, NL.
For the past 35 years Needham's Lab has developed and used a platform technology of micropipette manipulation to manipulate single and pairs of micro bubbles, droplets and particles in order to assess their behavior in well-defined fluids and solution conditions. Recently his research and development has focused on nucleation, growth and stability of nanoparticles. Applications of these fundamental particle and interfacial studies have primarily focused on advanced drug delivery treatments for cancer and now COVID19 with a nasal and throat spray prophylactic and early treatment regimen.
Current Research Interests
Needham’s research and development is nothing if it is not collaborative.
Highlights:
- Anti-cancer drug delivery inspired by how cancer's survive and spread due to their consumption of Lipoprotein particles from the blood-stream (LDLs and VLDLs) hence his approach:
- Make the drug look like the cancer's food" Converts low solubility drugs ("bricks") into even lower solubility "Rocks" that can be made into nanoparticles of pure drug made =by the solvent exchange process, hence his "Bricks to Rocks technology (B2RT)
- Explored the pH dependence of Niclosamide solubility, dissolution, and morphology and created a potentially universal mucin-penetrating nasal and throat sprays for COVID19, its viral variants and other viral infections.
- Single micro- and nano-particle science and engineering utilizing a micropipette technique for single particle micromanipulation.
For the past 35 years Needham's Lab has developed and used a platform technology of micropipette manipulation to manipulate single and pairs of micro particles in order to assess their behavior in well-defined fluids and solution conditions. With these techniques, he brings a wealth of expertise in colloid and interfacial science and engineering evaluating all 3 states of matter and their 5 interfaces, at the microscale, as micro-bubbles, -drops and -particles. Results of these kinds of studies have impacted: bubble technology for ultrasound; Droplet Interface Bilayers (DIBs) in collaboration with the Hagan Bayley lab at the University of Oxford UK; as well as the microglassification of proteins and peptides for storage, transport and use. This technology is now being developed by his ex-graduate student now CEO and Founder of Lindy Biosciences, Deborah Bitterfield PhD. Other industrial collaborations arising from the micropipet studies include with Gary Fujii, PhD, CEO and Founder of Molecular Express Inc in Los Angeles, California on a variety of products including a new lung surfactant for respiratory distress syndromes
Applications of these fundamental particle and interfacial studies have primarily focused on advanced drug delivery treatments for cancer. Of note over the past 20 years has been his invention and development of the Low-Temperature-Sensitive Liposome system for treatment of local cancers that can be warmed by mild hyperthermia. This 1996 invention was pre-clinically and clinically developed with collaborators in the Duke Medical Center, specifically with Dr. Mark Dewhirst PhD DVM (now retired) and his then Hyperthermia Program in Radiation Oncology and the Duke Cancer Institute and licensed to Celsion Corporation.
Recently his research and development has focused on nanoparticles, again for anti-cancer applications. Recognizing that cancers are known to “feed” on Low Density Lipoprotein (LDL) particles (--sub microscopic nanoparticles composed of fat and protein) from the blood stream, his new strategy is to “make the drug look like the cancer’s food”. The new technology turns the low solubility “bricks” of the pharmaceutical industry, that are very difficult to administer in effective doses by oral tablets, and turns them into much less soluble “rocks” so that they can be made into the LDL-sized nanoparticles for intravenous delivery. This work is in close collaboration with his colleague, Dave Gooden PhD, Director of the Duke Small Molecule Synthesis Facility; hence their “Bricks-to-Rocks Technology (B2RT). Here, in collaboration, with Will Eward DVM, MD Dept of Surgical Oncology and Ivan Spasojevic, PhD, Director of the PK/PD core, they have pioneered testing this new kind of anti-cancer drug nanoparticle. It has already shown positive results in a lung-metastatic mouse model of Osteosarcoma, as well as in a recent feasibility trial in canine patients with Steve Suter VMD, MS, PhD, DACVIM and colleagues at the NC State Veterinary School.
Finally, over the past year, responding to the COVID19 pandemic, Needham has developed 11 provisional patent applications and one in particular is now forming the basis for a new nasal and throat spray that is intended for use as a Prophylactic-Preventative and in early treatment of COVID19. This work is being carried out in a new collaboration with Christina BArkauskas, Assistant Professor in Pulmonary, Allergy and Critical Care Medicine, along with Dr Patty Lee and her lab. Together they are actively pursuing Investigational New Drug (IND) studies required to advance the new spray to testing in patients.
Current Appointments & Affiliations
- Professor in the Department of Mechanical Engineering and Materials Science, Thomas Lord Department of Mechanical Engineering and Materials Science, Pratt School of Engineering 1999
- Member of the Duke Cancer Institute, Duke Cancer Institute, Institutes and Centers 2010
Contact Information
- 3391 Fciemas Building, Box 90300, Durham, NC 27708
- 3391 Fciemas Building, Box 90300, Durham, NC 27708-0300
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d.needham@duke.edu
(919) 660-5355
- Background
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Education, Training, & Certifications
- Ph.D., University of Nottingham (United Kingdom) 1981
- B.S., Nottingham Trent University (United Kingdom) 1975
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Previous Appointments & Affiliations
- Professor of Biomedical Engineering, Biomedical Engineering, Pratt School of Engineering 2003 - 2004
- Professor of Biomedical Engineering, Biomedical Engineering, Pratt School of Engineering 1999 - 2000
- Associate Professor of Biomedical Engineering, Biomedical Engineering, Pratt School of Engineering 1997 - 1999
- Associate Professor with Tenure, Thomas Lord Department of Mechanical Engineering and Materials Science, Pratt School of Engineering 1993 - 1998
- Associate Professor, Biomedical Engineering, Pratt School of Engineering 1992 - 1994
- Associate Professor, Thomas Lord Department of Mechanical Engineering and Materials Science, Pratt School of Engineering 1991 - 1993
- Assistant Professor, Thomas Lord Department of Mechanical Engineering and Materials Science, Pratt School of Engineering 1987 - 1990
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Academic Positions Outside Duke
- Professor of Translational Therapeutics, University of Nottingham, UK. 2018 - 2023
- Recognition
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In the News
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JAN 12, 2022 Pratt School of Engineering -
JAN 12, 2017 -
JUL 18, 2014
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Awards & Honors
- Doctor of Science. University of Nottingham. December 2015
- Honorary Professor. School of Pharmacy, Nottingham University, Nottingham, UK. December 2014
- Hans Christian Andersen Academy Professor. University Southern Denmark, Odense, Denmark. 2012
- Niels Bohr Professorship,. Danish National Research Foundation, Denmark. January 2012
- VELUX Visiting Professor Program. VELUX Foundation. 2011
- Visiting Professor, MEMPHYS/MultiCaps. University of Southern Denmark, Denmark. 2011
- Participant: Series of Workshops and Tutorials on “Cells and Materials at the Interface between Mathematics, Biology and Engineering”. Institute for Pure and Applied Math, UCLA. March 2006
- National Academy of Sciences Committee on Biomolecular Materials and Processes. Board on Physics and Astronomy National Research Council, the National Academies . 2006
- Participant, “Blue Sky Committee” . Duke University. 2006
- Alfred M. Hunt Faculty Scholarship. Duke University. 1988
- Recipient F.I.R.S.T. Award. National Institutes of Health. 1988
- NATO/SERC (England) Fellowship. NATO/SERC . 1983
- Oppenheimer Research Fellowship. Cambridge University. 1982
- Expertise
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Subject Headings
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Global Scholarship
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Research
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Teaching
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- Research
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Selected Grants
- Niclosamide spray as a prophylactic and early treatment for SARS-CoV-2 awarded by American Lung Association 2021 - 2023
- TRP and AQP Channels: Modulation of Function, Raft Location by Membrane Lipids awarded by National Institutes of Health 1980 - 2015
- PLGA Protein Microspheres: Single Particle Engineering awarded by National Institutes of Health 2006 - 2011
- Faculty Development Workshop: "Course Enhancement Projects" Across the Pratt Curriculum awarded by Lord Foundation of North Carolina 2009 - 2010
- Integrating Nanoscale Systems and Design into the Undergraduate Engineering and Science Curricula awarded by National Science Foundation 2007 - 2010
- Training in Biomolecular and Tissue Engineering awarded by National Institutes of Health 2003 - 2009
- Ultra High-speed Imaging System awarded by National Institutes of Health 2003 - 2008
- Graduate training in Biologically Inspired Materials awarded by National Science Foundation 2002 - 2007
- Thermally Sensitive Drug Delivery System for Tumors awarded by National Institutes of Health 2000 - 2006
- Molecular Exchange with Lipid Membranes awarded by National Institutes of Health 1995 - 2005
- Engineering Biology at the Nanoscale awarded by National Institutes of Health 2004 - 2005
- Upgrade of a Shared Instrumentation Resource in the PSOE: The Laser Scanning Confocal Microscope awarded by Lord Foundation of North Carolina 2002 - 2005
- Development and Construction of Single Molecule Spectrometers for Research and Student Training awarded by National Science Foundation 2001 - 2003
- Molecular Forces in Blood-vasular Cell Interaction awarded by National Institutes of Health 1995 - 2000
- Molecular Forces in Blood/Vascular Cell Adhesion awarded by National Institutes of Health 1995 - 2000
- Molecular Exchange and Defect Formation in Membranes awarded by National Institutes of Health 1988 - 1999
- Particle Size Measurement: Instrumentation Grant awarded by Lord Foundation of North Carolina 1998 - 1999
- Molecular Exchange and Defect Formation in Membranes awarded by National Institutes of Health 1988 - 1999
- Molecular Exchange and Defect Formation in Membranes awarded by National Institutes of Health 1988 - 1999
- ME265.03 Biological Materials Sci; Curriculum Development & Textbook... awarded by Lord Foundation of North Carolina 1997 - 1998
- Viscoelasticity of Blood Cells awarded by National Institutes of Health 1979 - 1995
- Viscoelasticity of Blood Cells awarded by National Institutes of Health 1979 - 1995
- Biorheology of Hybridomas and Cell Damage awarded by National Science Foundation 1991 - 1994
- Electropermeabilitization/ Fusion of Lipid Vesicles and Cells awarded by National Institutes of Health 1988 - 1993
- Electropermeabilization/Fusion of Lipid Vesicles & Cells awarded by National Institutes of Health 1988 - 1993
- Electropermeabilization/Fusion of Lipid Vesicles and Cells awarded by National Institutes of Health 1988 - 1993
- Electropermeabilization/Fusion of Lipid Vesicles and Cells awarded by National Institutes of Health 1988 - 1993
- Electropermeabilization/Fusion of Liquid Vesicles and Cells awarded by National Institutes of Health 1988 - 1993
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Fellowships, Supported Research, & Other Grants
- Laboratory-to-Clinic Translational Research and Development in Cancer awarded by University of Nottingham 2018 - 2023
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External Relationships
- Molecualr Express Inc
- Needham Material Science, LLC
- Stabican, The Netherlands
- University of Nottingham, Nottingham, UK
- Publications & Artistic Works
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Selected Publications
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Academic Articles
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Needham, David. “The pH Dependence of Niclosamide Solubility, Dissolution, and Morphology: Motivation for Potentially Universal Mucin-Penetrating Nasal and Throat Sprays for COVID19, its Variants and other Viral Infections.” Pharmaceutical Research 39, no. 1 (January 2022): 115–41. https://doi.org/10.1007/s11095-021-03112-x.Full Text Open Access Copy
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Dudiak, Brianne M., Tri M. Nguyen, David Needham, Taylor C. Outlaw, and Dewey G. McCafferty. “Inhibition of the futalosine pathway for menaquinone biosynthesis suppresses Chlamydia trachomatis infection.” Febs Letters 595, no. 24 (December 2021): 2995–3005. https://doi.org/10.1002/1873-3468.14223.Full Text Open Access Copy
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Needham, David. “The pH Dependence of Niclosamide Solubility, Dissolution, and Morphology Motivates Potentially Universal Mucin-Penetrating Nasal and Throat Sprays for COVID19, its Contagious Variants, and Other Respiratory Viral Infections,” August 17, 2021. https://doi.org/10.1101/2021.08.16.456531.Full Text Open Access Copy
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Amer, Mahetab H., Marta Alvarez-Paino, Jane McLaren, Francesco Pappalardo, Sara Trujillo, Jing Qian Wong, Sumana Shrestha, et al. “Designing topographically textured microparticles for induction and modulation of osteogenesis in mesenchymal stem cell engineering.” Biomaterials 266 (January 2021): 120450. https://doi.org/10.1016/j.biomaterials.2020.120450.Full Text Open Access Copy
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Reddy, Gireesh B., David L. Kerr, Ivan Spasojevic, Artak Tovmasyan, David S. Hsu, Brian E. Brigman, Jason A. Somarelli, David Needham, and William C. Eward. “Preclinical Testing of a Novel Niclosamide Stearate Prodrug Therapeutic (NSPT) Shows Efficacy Against Osteosarcoma.” Mol Cancer Ther 19, no. 7 (July 2020): 1448–61. https://doi.org/10.1158/1535-7163.MCT-19-0689.Full Text Open Access Copy Link to Item
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Alvarez-Paino, Marta, Mahetab H. Amer, Aishah Nasir, Valentina Cuzzucoli Crucitti, Jordan Thorpe, Laurence Burroughs, David Needham, et al. “Polymer Microparticles with Defined Surface Chemistry and Topography Mediate the Formation of Stem Cell Aggregates and Cardiomyocyte Function.” Acs Applied Materials & Interfaces 11, no. 38 (September 2019): 34560–74. https://doi.org/10.1021/acsami.9b04769.Full Text
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Munk, M. B., A. Utoft, F. H. Larsen, D. Needham, and J. Risbo. “Oleogelating properties of ethylcellulose in oil-in-water emulsions: The impact of emulsification methods studied by 13C MAS NMR, surface tension and micropipette manipulation studies.” Food Hydrocolloids 89 (April 1, 2019): 700–706. https://doi.org/10.1016/j.foodhyd.2018.11.019.Full Text
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Needham, David, Koji Kinoshita, and Anders Utoft. “Micro-Surface and -Interfacial Tensions Measured Using the Micropipette Technique: Applications in Ultrasound-Microbubbles, Oil-Recovery, Lung-Surfactants, Nanoprecipitation, and Microfluidics.” Micromachines 10, no. 2 (February 2019): E105. https://doi.org/10.3390/mi10020105.Full Text
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Pipó-Ollé, Emma, Prasad Walke, Martine K. Notabi, Rime B. El-Houri, Morten Østergaard Andersen, David Needham, and Eva C. Arnspang. “Uptake of New Lipid-coated Nanoparticles Containing Falcarindiol by Human Mesenchymal Stem Cells.” Journal of Visualized Experiments : Jove, no. 144 (February 2019). https://doi.org/10.3791/59094.Full Text
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Hervella, Pablo, Johan Hygum Dam, Helge Thisgaard, Christina Baun, Birgitte Brinkmann Olsen, Poul Flemming Høilund-Carlsen, and David Needham. “Chelation, formulation, encapsulation, retention, and in vivo biodistribution of hydrophobic nanoparticles labelled with 57Co-porphyrin: Oleylamine ensures stable chelation of cobalt in nanoparticles that accumulate in tumors.” Journal of Controlled Release : Official Journal of the Controlled Release Society 291 (December 2018): 11–25. https://doi.org/10.1016/j.jconrel.2018.09.027.Full Text
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Utoft, Anders, Koji Kinoshita, Deborah L. Bitterfield, and David Needham. “Manipulating Single Microdroplets of NaCl Solutions: Solvent Dissolution, Microcrystallization, and Crystal Morphology.” Langmuir : The Acs Journal of Surfaces and Colloids 34, no. 12 (March 2018): 3626–41. https://doi.org/10.1021/acs.langmuir.7b03977.Full Text
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Kinoshita, Koji, Elisa Parra, and David Needham. “Adsorption of ionic surfactants at microscopic air-water interfaces using the micropipette interfacial area-expansion method: Measurement of the diffusion coefficient and renormalization of the mean ionic activity for SDS.” Journal of Colloid and Interface Science 504 (October 2017): 765–79. https://doi.org/10.1016/j.jcis.2017.05.077.Full Text
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Parra, Elisa, Pablo Hervella, and David Needham. “Real-Time Visualization of the Precipitation and Phase Behavior of Octaethylporphyrin in Lipid Microparticles.” Journal of Pharmaceutical Sciences 106, no. 4 (April 2017): 1025–41. https://doi.org/10.1016/j.xphs.2016.11.019.Full Text
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Kinoshita, Koji, Elisa Parra, and David Needham. “New sensitive micro-measurements of dynamic surface tension and diffusion coefficients: Validated and tested for the adsorption of 1-Octanol at a microscopic air-water interface and its dissolution into water.” Journal of Colloid and Interface Science 488 (February 2017): 166–79. https://doi.org/10.1016/j.jcis.2016.10.052.Full Text
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Yi, Jinsoo, Tine A. Knudsen, Anne-Louise Nielsen, Lars Duelund, Morten Christensen, Pablo Hervella, David Needham, and Ole G. Mouritsen. “Data for the size of cholesterol-fat micelles as a function of bile salt concentration and the physico-chemical properties of six liquid experimental pine-derived phytosterol formulations in a cholesterol-containing artificial intestine fluid.” Data in Brief 10 (February 2017): 478–81. https://doi.org/10.1016/j.dib.2016.12.027.Full Text
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Bitterfield, Deborah L., Anders Utoft, and David Needham. “An Activity-Based Dissolution Model for Solute-Containing Microdroplets.” Langmuir : The Acs Journal of Surfaces and Colloids 32, no. 48 (December 2016): 12749–59. https://doi.org/10.1021/acs.langmuir.6b03126.Full Text
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Kinoshita, K., E. Parra, A. Hussein, A. Utoft, P. Walke, R. de Bruijn, and D. Needham. “From single microparticles to microfluidic emulsification: Fundamental properties (solubility, density, phase separation) from micropipette manipulation of solvent, drug and polymer microspheres.” Processes 4, no. 4 (December 1, 2016). https://doi.org/10.3390/pr4040049.Full Text
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Needham, David, Amina Arslanagic, Kasper Glud, Pablo Hervella, Leena Karimi, Poul-Flemming Høeilund-Carlsen, Koji Kinoshita, et al. “Bottom up design of nanoparticles for anti-cancer diapeutics: "put the drug in the cancer's food".” Journal of Drug Targeting 24, no. 9 (November 2016): 836–56. https://doi.org/10.1080/1061186x.2016.1238092.Full Text
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Parra, Elisa, Koji Kinoshita, and David Needham. “Micropipette Technique Study of Natural and Synthetic Lung Surfactants at the Air-Water Interface: Presence of a SP-B Analog Peptide Promotes Membrane Aggregation, Formation of Tightly Stacked Lamellae, and Growth of Myelin Figures.” Langmuir : The Acs Journal of Surfaces and Colloids 32, no. 41 (October 2016): 10570–81. https://doi.org/10.1021/acs.langmuir.6b01420.Full Text
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Yi, Jinsoo, Tine A. Knudsen, Anne-Louise Nielsen, Lars Duelund, Morten Christensen, Pablo Hervella, David Needham, and Ole G. Mouritsen. “Inhibition of cholesterol transport in an intestine cell model by pine-derived phytosterols.” Chemistry and Physics of Lipids 200 (October 2016): 62–73. https://doi.org/10.1016/j.chemphyslip.2016.06.008.Full Text
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Hervella, Pablo, Elisa Parra, and David Needham. “Encapsulation and retention of chelated-copper inside hydrophobic nanoparticles: Liquid cored nanoparticles show better retention than a solid core formulation.” European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V 102 (May 2016): 64–76. https://doi.org/10.1016/j.ejpb.2016.02.015.Full Text
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Aniket, Ashley W., David A. Gaul, Deborah L. Bitterfield, Jonathan T. Su, Victoria M. Li, Ishita Singh, Jackson Morton, and David Needham. “Enzyme dehydration using Microglassification™ preserves the protein's structure and function.” Journal of Pharmaceutical Sciences 104, no. 2 (February 2015): 640–51. https://doi.org/10.1002/jps.24279.Full Text
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Zagar, Timothy M., Zeljko Vujaskovic, Silvia Formenti, Hope Rugo, Franco Muggia, Brigid O’Connor, Robert Myerson, et al. “Two phase I dose-escalation/pharmacokinetics studies of low temperature liposomal doxorubicin (LTLD) and mild local hyperthermia in heavily pretreated patients with local regionally recurrent breast cancer.” Int J Hyperthermia 30, no. 5 (August 2014): 285–94. https://doi.org/10.3109/02656736.2014.936049.Full Text Link to Item
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Bagatolli, Luis A., and David Needham. “Quantitative optical microscopy and micromanipulation studies on the lipid bilayer membranes of giant unilamellar vesicles.” Chemistry and Physics of Lipids 181 (July 2014): 99–120. https://doi.org/10.1016/j.chemphyslip.2014.02.009.Full Text
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Aniket, Daniel T., David A. Gaul, Deborah L. Rickard, and David Needham. “Microglassification™: a novel technique for protein dehydration.” Journal of Pharmaceutical Sciences 103, no. 3 (March 2014): 810–20. https://doi.org/10.1002/jps.23847.Full Text
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Olsen, B. B., H. Thisgaard, S. Vogel, M. Thomassen, T. A. Kruse, D. Needham, J. Mollenhauer, and P. Flemming Høilund-Carlsen. “Novel radioisotope-based nanomedical approaches.” European Journal of Nanomedicine 5, no. 4 (December 1, 2013): 181–93. https://doi.org/10.1515/ejnm-2013-0025.Full Text
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Needham, D., and M. W. Dewhirst. “Materials science and engineering of the low temperature sensitive liposome (LTSL): Composition-structure-property relationships that underlie its design and performance.” Rsc Smart Materials 1, no. 1 (November 25, 2013): 33–79.
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Su, Jonathan T., and David Needham. “Mass transfer in the dissolution of a multicomponent liquid droplet in an immiscible liquid environment.” Langmuir : The Acs Journal of Surfaces and Colloids 29, no. 44 (November 2013): 13339–45. https://doi.org/10.1021/la402533j.Full Text
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Davis, Ryan M., Benjamin L. Viglianti, Pavel Yarmolenko, Ji-Young Park, Paul Stauffer, David Needham, and Mark W. Dewhirst. “A method to convert MRI images of temperature change into images of absolute temperature in solid tumours.” Int J Hyperthermia 29, no. 6 (September 2013): 569–81. https://doi.org/10.3109/02656736.2013.790091.Full Text Link to Item
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Needham, David, Ji-Young Park, Alexander M. Wright, and Jihong Tong. “Materials characterization of the low temperature sensitive liposome (LTSL): effects of the lipid composition (lysolipid and DSPE-PEG2000) on the thermal transition and release of doxorubicin.” Faraday Discussions 161 (2013): 515–34. https://doi.org/10.1039/c2fd20111a.Full Text Link to Item
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Manzoor, Ashley A., Lars H. Lindner, Chelsea D. Landon, Ji-Young Park, Andrew J. Simnick, Matthew R. Dreher, Shiva Das, et al. “Overcoming limitations in nanoparticle drug delivery: triggered, intravascular release to improve drug penetration into tumors.” Cancer Res 72, no. 21 (November 1, 2012): 5566–75. https://doi.org/10.1158/0008-5472.CAN-12-1683.Full Text Link to Item
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Gilchrist, Samuel E., Deborah L. Rickard, Kevin Letchford, David Needham, and Helen M. Burt. “Phase separation behavior of fusidic acid and rifampicin in PLGA microspheres.” Molecular Pharmaceutics 9, no. 5 (May 2012): 1489–1501. https://doi.org/10.1021/mp300099f.Full Text
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Needham, David. “Lipid structures: a brief history of multisomes.” Nature Nanotechnology 6, no. 12 (December 2011): 761–62. https://doi.org/10.1038/nnano.2011.218.Full Text
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Grant, Gerald A., Christy Wilson, Sara Salami, Paolo Macaroni, Shuqin Li, Ji-Young Park, David Needham, Darell Bigner, and Mark Dewhirst. “PRECLINICAL USE OF MILD HYPERTHERMIA TO ENHANCE DRUG DELIVERY TO BRAIN TUMORS.” Neuro Oncology 13 (November 1, 2011): 111–111.Link to Item
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Landon, Chelsea D., Ji-Young Park, David Needham, and Mark W. Dewhirst. “Nanoscale Drug Delivery and Hyperthermia: The Materials Design and Preclinical and Clinical Testing of Low Temperature-Sensitive Liposomes Used in Combination with Mild Hyperthermia in the Treatment of Local Cancer.” Open Nanomed J 3 (January 1, 2011): 38–64. https://doi.org/10.2174/1875933501103010038.Full Text Link to Item
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Rickard, Deborah L., P Brent Duncan, and David Needham. “Hydration potential of lysozyme: protein dehydration using a single microparticle technique.” Biophysical Journal 98, no. 6 (March 2010): 1075–84. https://doi.org/10.1016/j.bpj.2009.11.043.Full Text
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Su, Jonathan T., P Brent Duncan, Amit Momaya, Arimatti Jutila, and David Needham. “The effect of hydrogen bonding on the diffusion of water in n-alkanes and n-alcohols measured with a novel single microdroplet method.” The Journal of Chemical Physics 132, no. 4 (January 2010): 044506. https://doi.org/10.1063/1.3298857.Full Text Open Access Copy
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Yarmolenko, Pavel S., Yulin Zhao, Chelsea Landon, Ivan Spasojevic, Fan Yuan, David Needham, Benjamin L. Viglianti, and Mark W. Dewhirst. “Comparative effects of thermosensitive doxorubicin-containing liposomes and hyperthermia in human and murine tumours.” Int J Hyperthermia 26, no. 5 (2010): 485–98. https://doi.org/10.3109/02656731003789284.Full Text Link to Item
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Yang, C. M., D. Plackett, D. Needham, and H. M. Burt. “PLGA and PHBV Microsphere Formulations and Solid-State Characterization: Possible Implications for Local Delivery of Fusidic Acid for the Treatment and Prevention of Orthopaedic Infections.” Pharmaceutical Research 26, no. 7 (July 2009): 1644–56.
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Chen, Qing, Ava Krol, Alex Wright, David Needham, Mark W. Dewhirst, and Fan Yuan. “Tumor microvascular permeability is a key determinant for antivascular effects of doxorubicin encapsulated in a temperature sensitive liposome.” Int J Hyperthermia 24, no. 6 (September 2008): 475–82. https://doi.org/10.1080/02656730701854767.Full Text Link to Item
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Tashjian, Jessica A., Mark W. Dewhirst, David Needham, and Benjamin L. Viglianti. “Rationale for and measurement of liposomal drug delivery with hyperthermia using non-invasive imaging techniques.” Int J Hyperthermia 24, no. 1 (February 2008): 79–90. https://doi.org/10.1080/02656730701840147.Full Text Link to Item
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Montalvo-Ortiz, Brenda Liz, Brian Sosa, Denisse Velez, David Needham, and Kai Griebenow. “Novel encapsulation of partially dehydrated protein microparticles in PLGA microspheres.” Journal of Biotechnology 131, no. 2 (September 2007): S51–52. https://doi.org/10.1016/j.jbiotec.2007.07.084.Full Text
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Holden, Matthew A., David Needham, and Hagan Bayley. “Functional bionetworks from nanoliter water droplets.” Journal of the American Chemical Society 129, no. 27 (July 2007): 8650–55. https://doi.org/10.1021/ja072292a.Full Text
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Simon, Scott I., Tun Nyunt, Kathryn Florine-Casteel, Ken Ritchie, H. P. Ting-Beall, Evan Evans, and David Needham. “Dynamics of neutrophil membrane compliance and microstructure probed with a micropipet-based piconewton force transducer.” Annals of Biomedical Engineering 35, no. 4 (April 2007): 595–604. https://doi.org/10.1007/s10439-007-9260-7.Full Text
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Mills, J. K., and D. Needham. “Temperature-triggered nanotechnology for chemotherapy: Rapid release from lysolipid temperature-sensitive liposomes.” 2006 Nsti Nanotechnology Conference and Trade Show Nsti Nanotech 2006 Technical Proceedings 2 (December 8, 2006): 5–8.
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Wei, Chiming, Yuri L. Lyubchenko, Hamid Ghandehari, Justin Hanes, Kathleen J. Stebe, Hai-Quan Mao, Donald T. Haynie, et al. “New technology and clinical applications of nanomedicine: highlights of the second annual meeting of the American Academy of Nanomedicine (Part I).” Nanomedicine : Nanotechnology, Biology, and Medicine 2, no. 4 (December 2006): 253–63. https://doi.org/10.1016/j.nano.2006.11.001.Full Text
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Hauck, Marlene L., Susan M. LaRue, William P. Petros, Jean M. Poulson, Daohai Yu, Ivan Spasojevic, Amy F. Pruitt, et al. “Phase I trial of doxorubicin-containing low temperature sensitive liposomes in spontaneous canine tumors.” Clin Cancer Res 12, no. 13 (July 1, 2006): 4004–10. https://doi.org/10.1158/1078-0432.CCR-06-0226.Full Text Link to Item
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Ponce, Ana M., Zeljko Vujaskovic, Fan Yuan, David Needham, and Mark W. Dewhirst. “Hyperthermia mediated liposomal drug delivery.” Int J Hyperthermia 22, no. 3 (May 2006): 205–13. https://doi.org/10.1080/02656730600582956.Full Text Link to Item
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Duncan, P Brent, and David Needham. “Microdroplet dissolution into a second-phase solvent using a micropipet technique: test of the Epstein-Plesset model for an aniline-water system.” Langmuir : The Acs Journal of Surfaces and Colloids 22, no. 9 (April 2006): 4190–97. https://doi.org/10.1021/la053314e.Full Text
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Ponce, Ana, Alex Wright, Mark Dewhirst, and David Needham. “Targeted bioavailability of drugs by triggered release from liposomes.” Future Lipidology 1, no. 1 (February 1, 2006): 25–34. https://doi.org/10.2217/17460875.1.1.25.Full Text
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Eichenbaum, K. D., A. A. Thomas, G. M. Eichenbaum, B. R. Gibney, D. Needham, and P. F. Kiser. “Oligo-α-hydroxy ester cross-linkers: Impact of cross-linker structure on biodegradable hydrogel networks.” Macromolecules 38, no. 26 (December 27, 2005): 10757–62. https://doi.org/10.1021/ma0518306.Full Text
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Mills, Jeffrey K., and David Needham. “Lysolipid incorporation in dipalmitoylphosphatidylcholine bilayer membranes enhances the ion permeability and drug release rates at the membrane phase transition.” Biochimica Et Biophysica Acta 1716, no. 2 (October 2005): 77–96. https://doi.org/10.1016/j.bbamem.2005.08.007.Full Text
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Merkin, J. H., D. J. Needham, and B. D. Sleeman. “A mathematical model for the spread of morphogens with density dependent chemosensitivity.” Nonlinearity (Uk) 18, no. 6 (2005): 2745–73. https://doi.org/10.1088/0951-7715/18/6/018.Full Text Link to Item
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Needham, D. J., J. A. Leach, and J. H. Merkin. “The effects of a complexation reaction on travelling wave-fronts in a quadratic autocatalytic system.” Q. J. Mech. Appl. Math. (Uk) 58 (2005): 577–99. https://doi.org/10.1093/qjmam/hbi022.Full Text Link to Item
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Dhawan, M., D. Price, D. Trump, P. Kanter, N. Shore, D. Needham, and M. Dewhirst. “New drug delivery approach for the treatment of prostate cancer (preclinical results and phase I study results).” Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology 22, no. 14_suppl (July 2004): 4661.
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Duncan, P Brent, and David Needham. “Test of the Epstein-Plesset model for gas microparticle dissolution in aqueous media: effect of surface tension and gas undersaturation in solution.” Langmuir : The Acs Journal of Surfaces and Colloids 20, no. 7 (March 2004): 2567–78. https://doi.org/10.1021/la034930i.Full Text
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Mills, Jeffrey K., and David Needham. “The materials engineering of temperature-sensitive liposomes.” Methods in Enzymology 387 (January 2004): 82–113. https://doi.org/10.1016/s0076-6879(04)87006-x.Full Text
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Kim, D. H., M. J. Costello, P. B. Duncan, and D. Needham. “Mechanical properties and microstructure of polycrystalline phospholipid monolayer shells: Novel solid microparticles.” Langmuir 19, no. 20 (September 30, 2003): 8455–66. https://doi.org/10.1021/la034779c.Full Text
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Ickenstein, Ludger M., Maria C. Arfvidsson, David Needham, Lawrence D. Mayer, and Katarina Edwards. “Disc formation in cholesterol-free liposomes during phase transition.” Biochimica Et Biophysica Acta 1614, no. 2 (August 2003): 135–38. https://doi.org/10.1016/s0005-2736(03)00196-2.Full Text
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Needham, D. “Lipid Membranes: Biological Inspiration for Micro and Nano Encapsulation Technologies, Especially Drug Delivery.” Materials Research Society Symposium Proceedings 774 (January 1, 2003): 173–202. https://doi.org/10.1557/proc-774-o5.6.Full Text
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Hasik, Matthew J., Dennis H. Kim, Laurens E. Howle, David Needham, and Donald P. Prush. “Evaluation of synthetic phospholipid ultrasound contrast agents.” Ultrasonics 40, no. 9 (November 2002): 973–82. https://doi.org/10.1016/s0041-624x(02)00384-0.Full Text
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Needham, D. S., I. C. K. Wong, P. D. Campion, and P. D. Hull and East Riding Pharmacy Developmnet Group. “Evaluation of the effectiveness of UK community pharmacists' interventions in community palliative care.” Palliative Medicine 16, no. 3 (May 2002): 219–25. https://doi.org/10.1191/0269216302pm533oa.Full Text
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Zhelev, D. V., and D. Needham. “Interactions of pH-sensitive peptides and polymers with lipid bilayers: Binding and membrane stability.” Current Topics in Membranes 52 (January 1, 2002): 437–64. https://doi.org/10.1016/s1063-5823(02)52017-2.Full Text
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Needham, D., and M. W. Dewhirst. “The development and testing of a new temperature-sensitive drug delivery system for the treatment of solid tumors.” Adv Drug Deliv Rev 53, no. 3 (December 31, 2001): 285–305. https://doi.org/10.1016/s0169-409x(01)00233-2.Full Text Link to Item
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Lee, S., D. H. Kim, and D. Needham. “Equilibrium and dynamic interfacial tension measurements at microscopic interfaces using a micropipet technique - 2. Dynamics of phospholipid monolayer formation and equilibrium tensions at the water-air interface.” Langmuir 17, no. 18 (September 4, 2001): 5544–50. https://doi.org/10.1021/la0103261.Full Text
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Lee, S., D. H. Kim, and D. Needham. “Equilibrium and Dynamic Interfacial Tension Measurements at Microscopic Interfaces Using a Micropipet Technique. 1. A New Method for Determination of Interfacial Tension.” Langmuir 17, no. 18 (September 4, 2001): 5537–43. https://doi.org/10.1021/la0103259.Full Text
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Zhelev, D. V., N. Stoicheva, P. Scherrer, and D. Needham. “Interaction of synthetic HA2 influenza fusion peptide analog with model membranes.” Biophysical Journal 81, no. 1 (July 2001): 285–304. https://doi.org/10.1016/s0006-3495(01)75699-8.Full Text
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Eichenbaum, Gary M., Patrick F. Kiser, Andrey V. Dobrynin, Sidney A. Simon, and David Needham. “Erratum: Investigation of the swelling response and loading of ionic microgels with drugs and proteins; The dependence on cross-link density (Macromolecules (July 27, 1999) 32:15 (4867-4878)).” Macromolecules 34, no. 18 (2001): 6526. https://doi.org/10.1021/ma0124892.Full Text Link to Item
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Eichenbaum, Gary M., Patrick F. Kiser, Sidney A. Simon, and David Needham. “Erratum: pH and ion-triggered volume responses of anionic hydrogel microspheres (Macromolecules (July 28, 1998) 31:15 (5084-5093)).” Macromolecules 34, no. 20 (2001): 7230. https://doi.org/10.1021/ma012488.Full Text Link to Item
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Kong, G., G. Anyarambhatla, W. P. Petros, R. D. Braun, O. M. Colvin, D. Needham, and M. W. Dewhirst. “Efficacy of liposomes and hyperthermia in a human tumor xenograft model: importance of triggered drug release.” Cancer Res 60, no. 24 (December 15, 2000): 6950–57.Link to Item
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Needham, D., and D. H. Kim. “PEG-covered lipid surfaces: bilayers and monolayers.” Colloids and Surfaces. B, Biointerfaces 18, no. 3–4 (October 2000): 183–95. https://doi.org/10.1016/s0927-7765(99)00147-2.Full Text
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Matteucci, M. L., G. Anyarambhatla, G. Rosner, C. Azuma, P. E. Fisher, M. W. Dewhirst, D. Needham, and D. E. Thrall. “Hyperthermia increases accumulation of technetium-99m-labeled liposomes in feline sarcomas.” Clin Cancer Res 6, no. 9 (September 2000): 3748–55.Link to Item
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Howell, M. L., E. Alsabbagh, J. F. Ma, U. A. Ochsner, M. G. Klotz, T. J. Beveridge, K. M. Blumenthal, et al. “AnkB, a periplasmic ankyrin-like protein in Pseudomonas aeruginosa, is required for optimal catalase B (KatB) activity and resistance to hydrogen peroxide.” Journal of Bacteriology 182, no. 16 (August 2000): 4545–56. https://doi.org/10.1128/jb.182.16.4545-4556.2000.Full Text
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Kiser, P. F., G. Wilson, and D. Needham. “Lipid-coated microgels for the triggered release of doxorubicin.” Journal of Controlled Release : Official Journal of the Controlled Release Society 68, no. 1 (July 2000): 9–22. https://doi.org/10.1016/s0168-3659(00)00236-4.Full Text
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Olbrich, K., W. Rawicz, D. Needham, and E. Evans. “Water permeability and mechanical strength of polyunsaturated lipid bilayers.” Biophys J 79, no. 1 (July 2000): 321–27. https://doi.org/10.1016/S0006-3495(00)76294-1.Full Text Link to Item
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Rawicz, W., K. C. Olbrich, T. McIntosh, D. Needham, and E. Evans. “Effect of chain length and unsaturation on elasticity of lipid bilayers.” Biophys J 79, no. 1 (July 2000): 328–39. https://doi.org/10.1016/S0006-3495(00)76295-3.Full Text Link to Item
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Eichenbaum, G. M., P. F. Kiser, D. Shah, W. P. Meuer, D. Needham, and S. A. Simon. “Alkali earth metal binding properties of ionic microgels.” Macromolecules 33, no. 11 (May 30, 2000): 4087–93. https://doi.org/10.1021/ma9917139.Full Text
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Kim, D. H., A. L. Klibanov, and D. Needham. “The influence of tiered layers of surface-grafted poly(ethylene glycol) on receptor-ligand-mediated adhesion between phospholipid monolayer-stabilized microbubbles and coated glass beads.” Langmuir 16, no. 6 (March 21, 2000): 2808–17. https://doi.org/10.1021/la990749r.Full Text
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Kiser, P. F., A. A. Thomas, G. M. Eichenbaum, D. Needham, and I. Kim. “Design and performance of poly(HPMA) hydrogels containing symmetrical biodegradable crosslinkers composed of oligo-lactate and oligo-glycolate esters.” American Chemical Society, Polymer Preprints, Division of Polymer Chemistry 41, no. 1 (March 1, 2000): 712–13.
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Needham, D., G. Anyarambhatla, G. Kong, and M. W. Dewhirst. “A new temperature-sensitive liposome for use with mild hyperthermia: characterization and testing in a human tumor xenograft model.” Cancer Res 60, no. 5 (March 1, 2000): 1197–1201.Link to Item
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Needham, D., S. Demurjian, and T. Peters. “An IDL to Ada95 mapping to support propagation modeling.” Ada Lett. (Usa) 20, no. 1 (2000): 58–66.
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Eichenbaum, G. M., P. F. Kiser, D. Shah, S. A. Simon, and D. Needham. “Investigation of the swelling response and drug loading of ionic microgels: The dependence on functional group composition.” Macromolecules 32, no. 26 (December 28, 1999): 8996–9006. https://doi.org/10.1021/ma9908393.Full Text
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Mills, J. K., and D. Needham. “Targeted drug delivery.” Expert Opinion on Therapeutic Patents 9, no. 11 (November 17, 1999): 1499–1513. https://doi.org/10.1517/13543776.9.11.1499.Full Text
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Eichenbaum, G. M., P. F. Kiser, A. V. Dobrynin, S. A. Simon, and D. Needham. “Investigation of the swelling response and loading of ionic microgels with drugs and proteins: the dependence on cross-link density.” Macromolecules 32, no. 15 (July 27, 1999): 4867–78. https://doi.org/10.1021/ma981945s.Full Text
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Anyarambhatla, G. R., and D. Needham. “Enhancement of the phase transition permeability of DPPC liposomes by incorporation of MPPC: A new temperature-sensitive liposome for use with mild hyperthermia.” Journal of Liposome Research 9, no. 4 (January 1, 1999): 491–506. https://doi.org/10.3109/08982109909035549.Full Text
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Klibanov, A. L., H. Gu, J. K. Wojdyla, J. Wible, D. H. Kim, D. Needham, F. S. Villanueva, and G. H. Brandenburger. “Attachment of ligands to gas-filled microbubbles via PEG spacer and lipid residues anchored at the interface.” Proceedings of the Controlled Release Society, no. 26 (January 1, 1999): 124–25.
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Mills, J. K., G. Eichenbaum, and D. Needham. “Effect of bilayer cholesterol and surface grafted poly(ethylene glycol) on pH-induced release of contents from liposomes by poly(2-ethylacrylic acid).” Journal of Liposome Research 9, no. 2 (January 1, 1999): 275–90. https://doi.org/10.3109/08982109909024790.Full Text
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Needham, D. “Materials engineering of lipid bilayers for drug carrier performance.” Mrs Bulletin 24, no. 10 (January 1, 1999): 32–40. https://doi.org/10.1557/s0883769400053240.Full Text
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Eichenbaum, G. M., P. F. Kiser, S. A. Simon, and D. Needham. “pH and Ion-Triggered Volume Response of Anionic Hydrogel Microspheres.” Macromolecules 31, no. 15 (July 1998): 5084–93. https://doi.org/10.1021/ma970897t.Full Text
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Kiser, P. F., G. Wilson, and D. Needham. “A synthetic mimic of the secretory granule for drug delivery.” Nature 394, no. 6692 (July 1998): 459–62. https://doi.org/10.1038/28822.Full Text
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Needham, D. J., and S. McAllister. “Centre families in two-dimensional complex holomorphic dynamical systems.” Proc. R. Soc. Lond. A, Math. Phys. Eng. Sci. (Uk) 454, no. 1976 (1998): 2267–78. https://doi.org/10.1098/rspa.1998.0258.Full Text Link to Item
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Needham, D., J. Mills, and G. Eichenbaum. “Interactions between poly(2-ethylacrylic acid) and lipid bilayer membranes: effects of cholesterol and grafted poly(ethylene glycol).” Faraday Discussions, no. 111 (January 1998): 103–10. https://doi.org/10.1039/a808717b.Full Text
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Needham, D., T. J. McIntosh, S. A. Simon, and D. Zhelev. “Adsorption, molecular exchange and defect formation in membranes.” Current Opinion in Colloid and Interface Science 3, no. 5 (January 1, 1998): 511–17. https://doi.org/10.1016/S1359-0294(98)80026-5.Full Text
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Needham, D., and R. S. Sarpal. “Binding of paclitaxel to lipid interfaces: Correlations with interface compliance.” Journal of Liposome Research 8, no. 2 (January 1, 1998): 147–63. https://doi.org/10.3109/08982109809035523.Full Text
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Needham, D., N. Stoicheva, and D. V. Zhelev. “Exchange of monooleoylphosphatidylcholine as monomer and micelle with membranes containing poly(ethylene glycol)-lipid.” Biophysical Journal 73, no. 5 (November 1997): 2615–29. https://doi.org/10.1016/s0006-3495(97)78291-2.Full Text
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Needham, D., and D. Noppl-Simson. “Barrier properties of surface-grafted PEG for macromolecule-surface interactions.” American Chemical Society, Polymer Preprints, Division of Polymer Chemistry 38, no. 1 (April 1, 1997): 549–50.
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Kiser, P. F., D. Needham, and G. Wilson. “Mimicking the smart polymer matrix of the secretory granule: a new concept in controlled release drug delivery.” Polymeric Materials Science and Engineering, Proceedings of the Acs Division of Polymeric Materials Science and Engineering 76 (January 1, 1997): 226.
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Zhelev, D. V., and D. Needham. “Pore formation and pore dynamics in bilayer membranes.” American Society of Mechanical Engineers, Bioengineering Division (Publication) Bed 34 (December 1, 1996): 47–49.
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Evans, E., H. Bowman, A. Leung, D. Needham, and D. Tirrell. “Biomembrane templates for nanoscale conduits and networks.” Science (New York, N.Y.) 273, no. 5277 (August 1996): 933–35. https://doi.org/10.1126/science.273.5277.933.Full Text
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Noppl-Simson, D. A., and D. Needham. “Avidin-biotin interactions at vesicle surfaces: adsorption and binding, cross-bridge formation, and lateral interactions.” Biophysical Journal 70, no. 3 (March 1996): 1391–1401. https://doi.org/10.1016/s0006-3495(96)79697-2.Full Text
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Bowman, H., E. Evans, D. Needham, and D. A. Tirrell. “Nanometer-scale polymeric patterns and networks.” Polymeric Materials Science and Engineering, Proceedings of the Acs Division of Polymeric Materials Science and Engineering 74 (1996): 437.
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Needham, D. J., A. C. King, and J. H. Merkin. “Reaction-diffusion model for autocatalytic polymerization: II. The initial value problem.” Ima Journal of Applied Mathematics (Institute of Mathematics & Its Applications) 56, no. 1 (1996): 65–86.
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McIntosh, T. J., S. Advani, R. E. Burton, D. V. Zhelev, D. Needham, and S. A. Simon. “Experimental tests for protrusion and undulation pressures in phospholipid bilayers.” Biochemistry 34, no. 27 (July 11, 1995): 8520–32. https://doi.org/10.1021/bi00027a002.Full Text Link to Item
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Hristova, K., and D. Needham. “Phase Behavior of a Lipid/Polymer-Lipid Mixture in Aqueous Medium.” Macromolecules 28, no. 4 (July 1, 1995): 991–1002. https://doi.org/10.1021/ma00108a029.Full Text
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Kenworthy, A. K., K. Hristova, D. Needham, and T. J. McIntosh. “Range and magnitude of the steric pressure between bilayers containing phospholipids with covalently attached poly(ethylene glycol).” Biophys J 68, no. 5 (May 1995): 1921–36. https://doi.org/10.1016/S0006-3495(95)80369-3.Full Text Link to Item
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Needham, D., and D. V. Zhelev. “Lysolipid exchange with lipid vesicle membranes.” Annals of Biomedical Engineering 23, no. 3 (May 1995): 287–98. https://doi.org/10.1007/bf02584429.Full Text
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Hristova, K., and D. Needham. “Influence of bilayer fluctuations on the steric interactions between polymer-grafted bilayers.” Liquid Crystals 18, no. 3 (January 1, 1995): 423–30. https://doi.org/10.1080/02678299508036641.Full Text
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Lasic, D. D., and D. Needham. “The "Stealth" liposome: A prototypical biomaterial.” Chemical Reviews 95, no. 8 (1995): 2601–28.
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Needham, D. J. “Centre theorem for two-dimensional complex holomorphic systems and its generalization.” Proceedings of the Royal Society of London, Series A: Mathematical and Physical Sciences 450, no. 1939 (1995): 225–32.
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Needham, D., and D. Noppl. “Adsorption and adhesion at vesicle surfaces: the influence of grafted polymer on avidin-biotin binding and tests of receptor-mediated adhesion.” American Society of Mechanical Engineers, Bioengineering Division (Publication) Bed 29 (January 1, 1995): 331–32.
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Needham, D., and D. V. Zhelev. “Lysolipid exchange with vesicle membranes and the formation and evolution of porous defects.” Annals of Biomedical Engineering 23, no. 3 (1995): 287.
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Zhelev, D. V., D. Needham, and R. M. Hochmuth. “A novel micropipet method for measuring the bending modulus of vesicle membranes.” Biophysical Journal 67, no. 2 (August 1994): 720–27. https://doi.org/10.1016/s0006-3495(94)80530-2.Full Text
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Zhelev, D. V., D. Needham, and R. M. Hochmuth. “Role of the membrane cortex in neutrophil deformation in small pipets.” Biophysical Journal 67, no. 2 (August 1994): 696–705. https://doi.org/10.1016/s0006-3495(94)80529-6.Full Text
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Simon, S. A., E. A. Disalvo, K. Gawrisch, V. Borovyagin, E. Toone, S. S. Schiffman, D. Needham, and T. J. McIntosh. “Increased adhesion between neutral lipid bilayers: interbilayer bridges formed by tannic acid.” Biophys J 66, no. 6 (June 1994): 1943–58. https://doi.org/10.1016/S0006-3495(94)80988-9.Full Text Link to Item
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Hristova, K., and D. Needham. “The Influence of polymer-grafted lipids on the physical properties of lipid bilayers: A theoretical study.” Journal of Colloid and Interface Science 168, no. 2 (January 1, 1994): 302–14. https://doi.org/10.1006/jcis.1994.1424.Full Text
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Wu, N. Z., B. Klitzman, G. Rosner, D. Needham, and M. W. Dewhirst. “Measurement of material extravasation in microvascular networks using fluorescence video-microscopy.” Microvasc Res 46, no. 2 (September 1993): 231–53. https://doi.org/10.1006/mvre.1993.1049.Full Text Link to Item
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Wu, N. Z., D. Da, T. L. Rudoll, D. Needham, A. R. Whorton, and M. W. Dewhirst. “Increased microvascular permeability contributes to preferential accumulation of Stealth liposomes in tumor tissue.” Cancer Res 53, no. 16 (August 15, 1993): 3765–70.Link to Item
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Hochmuth, R. M., H. P. Ting-Beall, B. B. Beaty, D. Needham, and R. Tran-Son-Tay. “Viscosity of passive human neutrophils undergoing small deformations.” Biophysical Journal 64, no. 5 (May 1993): 1596–1601. https://doi.org/10.1016/s0006-3495(93)81530-3.Full Text
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Ting-Beall, H. P., D. Needham, and R. M. Hochmuth. “Volume and osmotic properties of human neutrophils.” Blood 81, no. 10 (May 1993): 2774–80.
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Kavanagh, B. D., B. E. Coffey, D. Needham, R. M. Hochmuth, and M. W. Dewhirst. “The effect of flunarizine on erythrocyte suspension viscosity under conditions of extreme hypoxia, low pH, and lactate treatment.” Br J Cancer 67, no. 4 (April 1993): 734–41. https://doi.org/10.1038/bjc.1993.134.Full Text Link to Item
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Zhelev, D. V., and D. Needham. “Tension-stabilized pores in giant vesicles: determination of pore size and pore line tension.” Biochimica Et Biophysica Acta 1147, no. 1 (April 1993): 89–104. https://doi.org/10.1016/0005-2736(93)90319-u.Full Text
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NEEDHAM, D., D. DA, T. L. RUDOLL, H. P. TINGBEALL, N. WU, and M. W. DEWHIRST. “PERMEABILITY OF IMPLANTED-TUMOR VASCULATURE TO POLYMER-GRAFTED LIPOSOMES.” Biophysical Journal 64, no. 2 (February 1, 1993): A338–A338.Link to Item
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Needham, D. “Measurement of interbilayer adhesion energies.” Methods in Enzymology 220 (January 1993): 111–29. https://doi.org/10.1016/0076-6879(93)20078-h.Full Text
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Sonkin, P. L., S. F. Freedman, D. Needham, K. M. Rao, and D. L. Hatchell. “Pentoxifylline modulates deformability, F-actin content, and superoxide anion production of polymorphonuclear leukocytes from diabetic cats.” Exp Eye Res 55, no. 6 (December 1992): 831–38. https://doi.org/10.1016/0014-4835(92)90009-h.Full Text Link to Item
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Needham, D., T. J. McIntosh, and D. D. Lasic. “Repulsive interactions and mechanical stability of polymer-grafted lipid membranes.” Biochim Biophys Acta 1108, no. 1 (July 8, 1992): 40–48. https://doi.org/10.1016/0005-2736(92)90112-y.Full Text Link to Item
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Needham, D., and R. M. Hochmuth. “A sensitive measure of surface stress in the resting neutrophil.” Biophysical Journal 61, no. 6 (June 1992): 1664–70. https://doi.org/10.1016/s0006-3495(92)81970-7.Full Text
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Simon, S. A., T. J. McIntosh, A. D. Magid, and D. Needham. “Modulation of the interbilayer hydration pressure by the addition of dipoles at the hydrocarbon/water interface.” Biophys J 61, no. 3 (March 1992): 786–99. https://doi.org/10.1016/S0006-3495(92)81883-0.Full Text Link to Item
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McIntosh, T. J., S. A. Simon, D. Needham, and C. H. Huang. “Structure and cohesive properties of sphingomyelin/cholesterol bilayers.” Biochemistry 31, no. 7 (February 25, 1992): 2012–20. https://doi.org/10.1021/bi00122a017.Full Text Link to Item
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McIntosh, T. J., S. A. Simon, D. Needham, and C. H. Huang. “Interbilayer interactions between sphingomyelin and sphingomyelin/cholesterol bilayers.” Biochemistry 31, no. 7 (February 25, 1992): 2020–24. https://doi.org/10.1021/bi00122a018.Full Text Link to Item
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Needham, D. J. “Formal theory concerning the generation and propagation of travelling wave-fronts in reaction-diffusion equations.” Quarterly Journal of Mechanics and Applied Mathematics 45, no. pt 3 (1992): 469–98.
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Needham, D., K. Hristova, T. J. Mcintosh, M. Dewhirst, N. Wu, and D. D. Lasic. “Polymer-grafted liposomes: Physical basis for the "stealth" property.” Journal of Liposome Research 2, no. 3 (January 1, 1992): 411–30. https://doi.org/10.3109/08982109209010218.Full Text
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Hochmuth, R. M., D. Needham, H. P. Ting-Beall, and D. Zhelev. “Deformation and flow of neutrophils in micropipets.” Annals of Biomedical Engineering 19, no. 5 (December 1, 1991): 568.
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Hochmuth, R. M., D. Needham, and H. P. Ting-Beall. “Deformation and flow of neutrophils and monocytes.” American Society of Mechanical Engineers, Bioengineering Division (Publication) Bed 20 (December 1, 1991): 417–19.
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Needham, D. “Morphology, geometry and mechanical properties of GAP A3 hybridoma cells as a function of the cell cycle.” Annals of Biomedical Engineering 19, no. 5 (December 1, 1991): 592–93.
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Needham, D., and T. J. McIntosh. “Mechanical and interactive properties of lipid membranes containing surface-bound polymer.” Annals of Biomedical Engineering 19, no. 5 (December 1, 1991): 593.
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Tran-Son-Tay, R., D. Needham, and R. M. Hochmuth. “Recovery of passive neutrophils after large deformation. Liquid drop model.” American Society of Mechanical Engineers, Bioengineering Division (Publication) Bed 20 (December 1, 1991): 421–24.
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Needham, D., H. P. Ting-Beall, and R. Tran-Son-Tay. “A physical characterization of GAP A3 hybridoma cells: morphology, geometry, and mechanical properties.” Biotechnology and Bioengineering 38, no. 8 (October 1991): 838–52. https://doi.org/10.1002/bit.260380806.Full Text
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Tran-Son-Tay, R., D. Needham, A. Yeung, and R. M. Hochmuth. “Time-dependent recovery of passive neutrophils after large deformation.” Biophysical Journal 60, no. 4 (October 1991): 856–66. https://doi.org/10.1016/s0006-3495(91)82119-1.Full Text
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Needham, D. “Possible role of cell cycle-dependent morphology, geometry, and mechanical properties in tumor cell metastasis.” Cell Biophysics 18, no. 2 (April 1991): 99–121. https://doi.org/10.1007/bf02989809.Full Text
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Needham, D., H. P. Ting-Beall, and R. Tran-Son-Tay. “Morphology and mechanical properties of gap A3 hybridoma cells as related to cell cycle.” American Society of Mechanical Engineers, Bioengineering Division (Publication) Bed 16 (December 1, 1990): 5–10.
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Needham, D., and R. S. Nunn. “Elastic deformation and failure of lipid bilayer membranes containing cholesterol.” Biophys J 58, no. 4 (October 1990): 997–1009. https://doi.org/10.1016/S0006-3495(90)82444-9.Full Text Link to Item
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Needham, D., and R. M. Hochmuth. “Rapid flow of passive neutrophils into a 4 microns pipet and measurement of cytoplasmic viscosity.” Journal of Biomechanical Engineering 112, no. 3 (August 1990): 269–76. https://doi.org/10.1115/1.2891184.Full Text
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Armstrong, M., D. Needham, D. L. Hatchell, and R. S. Nunn. “Effect of pentoxifylline on the flow of polymorphonuclear leukocytes through a model capillary.” Angiology 41, no. 4 (April 1990): 253–62. https://doi.org/10.1177/000331979004100401.Full Text Link to Item
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Hochmuth, R. M., and D. Needham. “The viscosity of neutrophils and their transit times through small pores.” Biorheology 27, no. 6 (January 1990): 817–28. https://doi.org/10.3233/bir-1990-27603.Full Text
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Needham, D., M. Armstrong, D. L. Hatchell, and R. S. Nunn. “Rapid deformation of "passive" polymorphonuclear leukocytes: the effects of pentoxifylline.” J Cell Physiol 140, no. 3 (September 1989): 549–57. https://doi.org/10.1002/jcp.1041400321.Full Text Link to Item
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Needham, D., and R. M. Hochmuth. “Electro-mechanical permeabilization of lipid vesicles. Role of membrane tension and compressibility.” Biophysical Journal 55, no. 5 (May 1989): 1001–9. https://doi.org/10.1016/s0006-3495(89)82898-x.Full Text
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Needham, D., and E. Evans. “Structure and mechanical properties of giant lipid (DMPC) vesicle bilayers from 20 degrees C below to 10 degrees C above the liquid crystal-crystalline phase transition at 24 degrees C.” Biochemistry 27, no. 21 (October 1988): 8261–69. https://doi.org/10.1021/bi00421a041.Full Text
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Needham, D., T. J. McIntosh, and E. Evans. “Thermomechanical and transition properties of dimyristoylphosphatidylcholine/cholesterol bilayers.” Biochemistry 27, no. 13 (June 28, 1988): 4668–73. https://doi.org/10.1021/bi00413a013.Full Text Link to Item
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Evans, E., and D. Needham. “Attraction between Lipid Bilayer Membranes in Concentrated Solutions of Nonadsorbing Polymers: Comparison of Mean-Field Theory with Measurements of Adhesion Energy.” Macromolecules 21, no. 6 (January 1, 1988): 1822–31. https://doi.org/10.1021/ma00184a049.Full Text
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Evans, E., and D. Needham. “Physical properties of surfactant bilayer membranes: Thermal transitions, elasticity, rigidity, cohesion, and colloidal interactions.” Journal of Physical Chemistry 91, no. 16 (1987): 4219–28.
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Evans, E., and D. Needham. “Giant vesicle bilayers composed of mixtures of lipids, cholesterol and polypeptides. Thermomechanical and (mutual) adherence properties.” Faraday Discussions of the Chemical Society, no. 81 (January 1986): 267–80. https://doi.org/10.1039/dc9868100267.Full Text
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Elliott, J. R., D. A. Haydon, B. M. Hendry, and D. Needham. “Inactivation of the sodium current in squid giant axons by hydrocarbons.” Biophysical Journal 48, no. 4 (October 1985): 617–22. https://doi.org/10.1016/s0006-3495(85)83817-0.Full Text
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Elliott, J. R., D. Needham, J. P. Dilger, O. Brandt, and D. A. Haydon. “A quantitative explanation of the effects of some alcohols on gramicidin single-channel lifetime.” Biochimica Et Biophysica Acta 814, no. 2 (April 1985): 401–4. https://doi.org/10.1016/0005-2736(85)90462-6.Full Text
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Eley, D. D., and D. Needham. “HYDRIDIZATION AND CATALYSIS BY LANTHANIDE FILMS.” Proceedings of the Royal Society of London, Series A: Mathematical and Physical Sciences 393, no. 1805 (1984): 257–76.
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Elliott, J. R., D. Needham, J. P. Dilger, and D. A. Haydon. “The effects of bilayer thickness and tension on gramicidin single-channel lifetime.” Biochimica Et Biophysica Acta 735, no. 1 (October 1983): 95–103. https://doi.org/10.1016/0005-2736(83)90264-x.Full Text
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Needham, D., and D. A. Haydon. “Tensions and free energies of formation of "solventless" lipid bilayers. Measurement of high contact angles.” Biophysical Journal 41, no. 3 (March 1983): 251–57. https://doi.org/10.1016/s0006-3495(83)84435-x.Full Text
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Book Sections
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Needham, D. “Development of clinically effective formulations for anticancer applications: why it is so difficult?” In Biomaterials for Cancer Therapeutics: Evolution and Innovation, 599–723, 2020. https://doi.org/10.1016/B978-0-08-102983-1.00022-3.Full Text
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Anibal Disalvo, E., and S. Simon. “Preface.” In Permeability and Stability of Lipid Bilayers, iii, 2017. https://doi.org/10.1201/9780203743805.Full Text
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Needham, D. “Bringing research to clinical application lessons from thermodox(r): A thermal-sensitive liposome for treatment of cancer.” In Drug Delivery: Fundamentals and Applications: Second Edition, 523–85, 2016. https://doi.org/10.1201/9781315382579.Full Text
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Needham, D. “Reverse engineering of the low temperature-sensitive liposome (LTSL) for treating cancer.” In Biomaterials for Cancer Therapeutics: Diagnosis, Prevention and Therapy, 270–348, 2013. https://doi.org/10.1533/9780857096760.3.270.Full Text
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Needham, D., and D. Zhelev. “Use of Micropipet Manipulation Techniques to Measure the Properties of Giant Lipid Vesicles.” In Giant Vesicles: Perspectives in Supramolecular Chemistry, 6:102–47, 2007. https://doi.org/10.1002/9780470511534.ch9.Full Text
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Conference Papers
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Brousell, Steven C., Thomas A. Longo, Joseph J. Fantony, Wiguins Etienne, David Needham, Mark W. Dewhirst, Paolo F. Maccarini, Ivan Spasojevic, and Brant A. Inman. “MP65-08 HEAT-TARGETED DRUG DELIVERY USING THE COMBAT BRS DEVICE FOR TREATING BLADDER CANCER.” In Journal of Urology, Vol. 197. Ovid Technologies (Wolters Kluwer Health), 2017. https://doi.org/10.1016/j.juro.2017.02.2002.Full Text
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Kinoshita, Koji, Kasper Glud, and David Needham. “New Measurements of Lung Surfactant Interfacial Tension with Micropipette Manipulation Technique.” In Biophysical Journal, 106:518a-518a. Elsevier BV, 2014. https://doi.org/10.1016/j.bpj.2013.11.2891.Full Text
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Rickard, Deborah L., P Brent Duncan, and David Needham. “Hydration Potential of Lysozyme: Protein Dehydration Using a Single Microparticle Technique.” In Biophysical Journal, 98:45a-45a. Elsevier BV, 2010. https://doi.org/10.1016/j.bpj.2009.12.259.Full Text
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Needham, D., A. Wright, J. Tong, M. W. Dewhirst, A. Ponce, and F. Yuan. “Mapping engineering onto biology at the nanoscale: nature's encapsulation technologies as bioinspiration for nano-scale anti-tumor drug delivery.” In Nanomedicine: Nanotechnology, Biology and Medicine, 2:292–93. Elsevier BV, 2006. https://doi.org/10.1016/j.nano.2006.10.077.Full Text
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Lee, S. H., D. Needham, P. B. Duncan, and A. Jutila. “Water micro-droplets in simple hydrocarbons: Dissolution, free energies of adhesion and bilayer permeability.” In Abstracts of Papers of the American Chemical Society, 229:U667–U667. AMER CHEMICAL SOC, 2005.Link to Item
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Dhawan, M., D. Price, D. Trump, P. Kanter, N. Shore, D. Needham, and M. Dewhirst. “New drug delivery approach for the treatment of prostate cancer (preclinical results and phase I study results).” In Journal of Clinical Oncology, 22:421S-421S. AMER SOC CLINICAL ONCOLOGY, 2004.Link to Item
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Needham, D. “From properties to performance: The mechanochemistry of lipid vesicles with implications for drug carrier design.” In Journal of Liposome Research, 13:103–4. MARCEL DEKKER INC, 2003.Link to Item
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Needham, D., and S. Lee. “From soft materials to rational design of active carrier delivery systems.” In Abstracts of Papers of the American Chemical Society, 224:U446–47. AMER CHEMICAL SOC, 2002.Link to Item
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Zhelev, D. V., N. G. Stoicheva, and D. Needham. “Interaction of the amphipatic peptide AcE4K with SOPC membranes.” In Biophysical Journal, 80:531A-531A. BIOPHYSICAL SOCIETY, 2001.Link to Item
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Kiser, P. F., A. A. Thomas, G. M. Eichenbaum, D. Needham, and I. Kim. “Design and performance of poly(HPMA) hydrogels containing symmetrical biodegradable cross-linkers composed of oligo-lactate and oligo-glycolate esters.” In Abstracts of Papers of the American Chemical Society, 219:U410–U410. AMER CHEMICAL SOC, 2000.Link to Item
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Dewhirst, M. W., G. Kong, and D. Needham. “Use of hyperthermia to augment liposomal drug delivery to tumours.” In Radiation Research, Vol 2, Congress Proceedings, edited by M. Moriaty, M. Edington, C. Mothersill, J. F. Ward, C. Seymour, and R. J. M. Fry, 853–56. ALLEN PRESS INC, 2000.Link to Item
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Stoicheva, N. G., D. V. Zhelev, and D. Needham. “Exchange of monooleoylphophatidylcholine with membranes containing cholesterol.” In Biophysical Journal, 78:184A-184A. BIOPHYSICAL SOCIETY, 2000.Link to Item
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Needham, D., and I. Wong. “An expert panel review to evaluate the effectiveness of community pharmacist's interventions in the palliative care setting.” In Pharmaceutical Journal, Vol. 263, 1999.
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Florine-Casteel, K., K. Ritchie, H. P. Ting-Beall, E. Evans, and D. Needham. “Nanomechanics of the neutrophil interface: Cell surface compliance.” In Biophysical Journal, 76:A96–A96. BIOPHYSICAL SOCIETY, 1999.Link to Item
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Eichenbaum, G. M., P. F. Kiser, S. A. Simon, and D. Needham. “Environmental response of anionic microgels.” In Abstracts of Papers of the American Chemical Society, 216:U871–U871. AMER CHEMICAL SOC, 1998.Link to Item
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Eichenbaum, G. M., P. F. Kiser, S. A. Simon, and D. Needham. “pH and NaCl triggered response of anionic microgels.” In Abstracts of Papers of the American Chemical Society, 216:U909–U909. AMER CHEMICAL SOC, 1998.Link to Item
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Florine-Casteel, K., K. Ritchie, E. Evans, and D. Needham. “Nanomechanics of the neutrophil interface: Cell surface compliance.” In Abstracts of Papers of the American Chemical Society, 216:U674–75. AMER CHEMICAL SOC, 1998.Link to Item
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Kim, D. H., A. L. Klibanov, E. A. Evans, and D. Needham. “Viscoelastic properties of phospholipid monolayer shells on air microbubbles.” In Biophysical Journal, 74:A313–A313. BIOPHYSICAL SOCIETY, 1998.Link to Item
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Needham, D., N. Stoicheva, and D. V. Zhelev. “Exchange of monooleoylphosphatidylcholine as monomer and micelle with membranes containing poly(ethylene glycol)-lipid.” In Biophysical Journal, 74:A393–A393. BIOPHYSICAL SOCIETY, 1998.Link to Item
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Olbrich, K. C., O. F. Hutter, and D. Needham. “Arachidonic acid increases water permeability of sarcolemmal vesicles from rabbit skeletal muscle.” In Biophysical Journal, 74:A393–A393. BIOPHYSICAL SOCIETY, 1998.Link to Item
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Zhelev, D. V., N. Stoicheva, and D. Needham. “Uptake of fuson peptide by bilayer membranes and mechanical properties of membranes containing fuson peptide.” In Biophysical Journal, 74:A221–A221. BIOPHYSICAL SOCIETY, 1998.Link to Item
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Evans, E., H. Bowman, A. Leung, D. Needham, and D. Tirrell. “Biomembrane templating of nanometer-scale polymer patterns.” In Abstracts of Papers of the American Chemical Society, 214:84-PMSE. AMER CHEMICAL SOC, 1997.Link to Item
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Kiser, P. F., D. Needham, and G. Wilson. “Mimicking the smart polymer matrix of the secretory granule: A new concept in controlled release drug delivery.” In Abstracts of Papers of the American Chemical Society, 213:134-PMSE. AMER CHEMICAL SOC, 1997.Link to Item
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Needham, D., and D. NopplSimson. “Barrier properties of surface-grafted PEG for macromolecule-surface interactions.” In Abstracts of Papers of the American Chemical Society, 213:61-POLY. AMER CHEMICAL SOC, 1997.Link to Item
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TingBeall, H. P., W. Kao, W. M. Valentine, and D. Needham. “Toxin-induced changes in cell mechanochemical properties.” In Faseb Journal, 11:3160–3160. FEDERATION AMER SOC EXP BIOL, 1997.Link to Item
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Bowman, H., E. Evans, D. Needham, and D. Tirrell. “Nanometer-scale polymeric patterns and networks.” In Abstracts of Papers of the American Chemical Society, 211:93-PMSE. AMER CHEMICAL SOC, 1996.Link to Item
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Noppl, D. A., and D. Needham. “Avidin-biotin interactions at vesicle surfaces: Adsorption and binding, cross bridge formation, and lateral interactions.” In Biophysical Journal, 70:SU359–SU359. CELL PRESS, 1996.Link to Item
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Olbrich, K. C., W. Rawicz, E. A. Evans, and D. Needham. “Water permeability and mechanical properties of polyunsaturated lipid membranes.” In Biophysical Journal, 70:SU340–SU340. CELL PRESS, 1996.Link to Item
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MCINTOSH, T. J., D. NEEDHAM, D. V. ZHELEV, and S. A. SIMON. “EXPERIMENTAL TESTS FOR MOLECULAR PROTRUSIONS AND UNDULATIONS IN LIPID BILAYERS.” In Abstracts of Papers of the American Chemical Society, 210:17-COLL. AMER CHEMICAL SOC, 1995.Link to Item
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HRISTOVA, K., and D. NEEDHAM. “INCORPORATION OF POLYMER-LIPIDS INTO BILAYERS LEADS TO PHASE-TRANSITION.” In Biophysical Journal, 66:A177–A177. BIOPHYSICAL SOCIETY, 1994.Link to Item
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KENWORTHY, A. K., T. J. MCINTOSH, K. HRISTOVA, and D. NEEDHAM. “STERIC INTERACTIONS BETWEEN GEL AND LIQUID-CRYSTALLINE PHOSPHATIDYLCHOLINE BILAYERS CONTAINING LIPIDS WITH COVALENTLY ATTACHED POLYETHYLENE-GLYCOL.” In Biophysical Journal, 66:A287–A287. BIOPHYSICAL SOCIETY, 1994.Link to Item
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HOCHMUTH, R. M., H. P. TINGBEALL, D. NEEDHAM, and R. TRANSONTAY. “RECOVERY OF PASSIVE HUMAN NEUTROPHILS AFTER SMALL DEFORMATIONS.” In Biophysical Journal, 64:A292–A292. BIOPHYSICAL SOCIETY, 1993.Link to Item
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HRISTOVA, K., and D. NEEDHAM. “THEORETICAL-STUDIES OF MODEL BIOSURFACE PROPERTIES.” In Biophysical Journal, 64:A298–A298. BIOPHYSICAL SOCIETY, 1993.Link to Item
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KENWORTHY, A. K., T. J. MCINTOSH, D. NEEDHAM, and K. HRISTOVA. “STERIC INTERACTIONS BETWEEN BILAYERS CONTAINING LIPIDS WITH COVALENTLY ATTACHED POLYETHYLENE-GLYCOL.” In Biophysical Journal, 64:A348–A348. BIOPHYSICAL SOCIETY, 1993.Link to Item
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ZHELEV, D. V., D. NEEDHAM, and R. M. HOCHMUTH. “A NOVEL MICROPIPET TECHNIQUE FOR STUDYING NEUTROPHIL LOCOMOTION, SPREADING AND CONTRACTILITY DURING PHAGOCYTOSIS AND MECHANICALLY STIMULATED POLYMERIZATION OF ACTIN NETWORK.” In Biophysical Journal, 64:A367–A367. BIOPHYSICAL SOCIETY, 1993.Link to Item
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ZHELEV, D. V., and D. NEEDHAM. “LONG LIVED REVERSIBLE PORES IN GIANT LIPOSOMES - A NOVEL METHOD FOR DETERMINATION OF PORE-SIZE AND PORE LINE TENSION.” In Biophysical Journal, 64:A297–A297. BIOPHYSICAL SOCIETY, 1993.Link to Item
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LASIC, D. D., K. MACLEOD, A. HUANG, R. M. ABRA, M. S. NEWMAN, F. J. MARTIN, D. NEEDHAM, and E. MAYHEW. “GRAFTED POLYMER (STEALTH) LIPOSOMES.” In Abstracts of Papers of the American Chemical Society, 203:51-BIOT. AMER CHEMICAL SOC, 1992.Link to Item
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NEEDHAM, D., T. J. MCINTOSH, and S. A. SIMON. “CONFORMATION AND INTERACTIVE PROPERTIES OF SURFACE-BOUND POLYETHYLENE-GLYCOL (PEG) IN LIPID-MEMBRANES AND MONOLAYERS.” In Faseb Journal, 6:A237–A237. FEDERATION AMER SOC EXP BIOL, 1992.Link to Item
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SONKIN, P. L., D. NEEDHAM, K. M. K. RAO, S. F. FREEDMAN, and D. L. HATCHELL. “PENTOXIFYLLINE IMPROVES DEFORMABILITY AND DECREASES F-ACTIN CONTENT OF PASSIVE POLYMORPHONUCLEAR LEUKOCYTES IN A CAT MODEL OF DIABETES.” In Investigative Ophthalmology & Visual Science, 32:1288–1288. LIPPINCOTT-RAVEN PUBL, 1991.Link to Item
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NEEDHAM, D., and R. S. NUNN. “COHESIVE PROPERTIES (ELASTIC-DEFORMATION AND FAILURE) OF LIPID BILAYERS CONTAINING CHOLESTEROL.” In Biophysical Journal, 57:A219–A219. BIOPHYSICAL SOCIETY, 1990.Link to Item
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NEEDHAM, D., and E. EVANS. “STRONG ATTRACTION INDUCED BETWEEN LIPID BILAYER-MEMBRANES BY NON-ADSORBING POLYMERS.” In Biophysical Journal, 53:A119–A119. BIOPHYSICAL SOCIETY, 1988.Link to Item
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- Teaching & Mentoring
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Recent Courses
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Teaching Activities
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FACILITATED LEARNING
Since 1987, Needham’s educational activities have included courses in: Surface and Colloid Science; Engineering Materials; Cellular and Biosurface Engineering; Colloids and Surfaces in Environmental Science and Engineering; Biological Materials Science; Soft Wet Materials and Interfaces; Biologically Inspired Materials and Materials Systems; Mapping Engineering onto Biology; Reverse Engineer Problems Nature Solved; Introduction to Engineering: “Problems Engineers Solved” for Undergraduate (Years 1-4) and Graduate (MS, PhD, Medical), and also K-12 (Middle School, I the Duke TIP); “Reverse Engineer Problems Pharmacists Solved” at SDU Denmark, and now at MEMS with Spring 2019 and Fall 2019 courses called “How to Reverse Engineer Anything”
Over the past 20 years, Needham has made a strong commitment to the Pratt undergrad and grad education. With an emphasis in Biological systems, he explored if and to what extent we could “Map Engineering onto Biology”, what that means, and what we would get out of it --vis a vis a more rigorous understanding of nature’s mechanisms, mainly in the human body, but also in the plant and other animal worlds. What started in the Fall of 2003, as an introductory and foundation course for the then newly funded National Science Foundation IGERT[1] program recruiting 11 graduate students, soon caught the attention of 24 BME undergraduates in the Fall of 2004, when the enrollment was then mixed with 13 graduate students. In the Fall of 2005, 52 students enrolled, and in the Spring 2006 they must have each told two friends and 92 students requested and took the class. Since then there has been a steady stream of 44 (Fall 2006), 61 (Spring 2007), and 72 (Fall 2007) student enrollment. Fall 2008 a course dedicated to just our Duke FOCUS program was offered and was taken by 13, first semester, freshman, who reverse engineered the five senses. Subsequent FOCUS courses have now included topics relevant to the NAEs 14 Grand Challenges, and Pratt’s commitment to its new Grand Challenge Scholar’s Program. The first is Mapping Engineering onto Biology for “How did Nature Solve the Process Sensory Inputs Problem?” (Reverse Engineer the Brain), (Fall 2009) and follows on from the 5 senses of 2008. The second is “How did Nature Solve its Transport Molecules in the Body Problem?” offered in Fall 2009 and 2010, (Engineer Better Medicines). Focused on Nature’s designs, the goal here is for students to create new inventions in drug delivery by a formal process of reverse engineering Nature’s own molecule transport systems. His EDU-K pedagogy of learning (Experiment, Discover, Uncover, leading to new Knowledge), and the content framework of design, in a large part also addresses a third grand challenge, “Personalized Learning”. Needham has also piloted the same kind of approaches in the summer of 2008 with Middle Schoolers in 2 sessions of our Duke Talent Identification Program, (Duke TIP).
Needham continues to broaden collaborations to introduce these methods to interested faculty and Institutions in the US and abroad, including, faculty at the Royal Danish School of Pharmacy, the University of Southern Denmark (2013-2015) “Reverse engineer problems Pharmacists solved” and has also taught the introductory course at the Pharmacy School in Nottingham UK (2017 and 2018)
His most recent offering is entitled "How to Reverse Engineer Anything as an INvention and Innovation Generator" offered as both an undergraduate and graduate course. A “learner-centric” experience that educates students for a lifetime. THe calls it his EDUK-pedagogy of learner-centric “EDUKation”, where, together with students in coaching sessions they Explore, Discover, Uncover principles with experts, leading to their (and his) new Knowledge. As they go around this again and again it's a spiral of ever-increasing knowledge, for the students.
[1] Interdisciplinary Graduate Education and Research Training, “Graduate Training in Biologically Inspired Materials” Co-P.I. with Prof Robert Clark P.I., $2,900,821, 12/15/02-12/14/07
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FACILITATED LEARNING
- Scholarly, Clinical, & Service Activities
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Presentations & Appearances
- How to Reverse Engineer Anything as an Invention and Innovation Generator: Anti-cancer treatments from bench-to-bedside and bench-to-kennels. Symposia: Scientific Excursions and Diversions series (moderator, Mike Smith). Duke Osher Life Long Learning Institute (OLLI) . April 6, 2021 2021
- The Primacy of Learning: A “Brief” overview of my evolving educational pedagogy. Graduate Student Seminar Series . Duke University. February 21, 2020 2020
- Reverse Engineering: How did Nature Solve the “deliver fats in the body” problem? and How can we use this in drug delivery?. Graduate Student Seminar Series . Duke University. November 15, 2019 2019
- Endogenous-Inspired Anti-Cancer Therapeutics: “Make the drug look like the cancer’s food”. DCI Prostate and Urologic Cancers forum. Duke University Medical School. September 10, 2019 2019
- Endogenous-Inspired Anti-Cancer Therapeutics: The twists and turns of the design process when one attempts to “make the drug look like the cancer’s food” . Department of Biological Sciences Seminar series. California State University, Los Angeles. April 15, 2019 2019
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