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Daniele Armaleo

Associate Professor of the Practice Emeritus of Biology
Biology
Box 90338, Durham, NC 27708-0338
0052 Bio Sci Bldg, Durham, NC 27708

Selected Publications


Depside and Depsidone Synthesis in Lichenized Fungi Comes into Focus through a Genome-Wide Comparison of the Olivetoric Acid and Physodic Acid Chemotypes of Pseudevernia furfuracea.

Journal Article Biomolecules · October 2021 Primary biosynthetic enzymes involved in the synthesis of lichen polyphenolic compounds depsides and depsidones are non-reducing polyketide synthases (NR-PKSs), and cytochrome P450s. However, for most depsides and depsidones the corresponding PKSs are unkn ... Full text Cite

Modeling in yeast how rDNA introns slow growth and increase desiccation tolerance in lichens.

Journal Article G3 (Bethesda, Md.) · October 2021 We connect ribosome biogenesis to desiccation tolerance in lichens, widespread symbioses between specialized fungi (mycobionts) and unicellular phototrophs. We test whether the introns present in the nuclear ribosomal DNA of lichen mycobionts contribute to ... Full text Cite

The lichen symbiosis re-viewed through the genomes of Cladonia grayi and its algal partner Asterochloris glomerata.

Journal Article BMC Genomics · July 23, 2019 BACKGROUND: Lichens, encompassing 20,000 known species, are symbioses between specialized fungi (mycobionts), mostly ascomycetes, and unicellular green algae or cyanobacteria (photobionts). Here we describe the first parallel genomic analysis of the mycobi ... Full text Link to item Cite

A method for simultaneous targeted mutagenesis of all nuclear rDNA repeats in Saccharomyces cerevisiae using CRISPR-Cas9

Journal Article bioRxiv · March 3, 2019 Saccharomyces cerevisiae has been the prime model to study the assembly and functionality of eukaryotic ribosomes. Within that vast landscape, the specific problem of mutagenizing all 150 nuclear rRNA genes was bypassed using strains whose chromosomal copi ... Full text Link to item Cite

Insights from the first putative biosynthetic gene cluster for a lichen depside and depsidone.

Journal Article Mycologia · July 2011 The genes for polyketide synthases (PKSs), enzymes that assemble the carbon backbones of many secondary metabolites, often cluster with other secondary pathway genes. We describe here the first lichen PKS cluster likely to be implicated in the biosynthesis ... Full text Cite

Fungal and algal gene expression in early developmental stages of lichen-symbiosis.

Journal Article Mycologia · March 2011 How plants and microbes recognize each other and interact to form long-lasting relationships remains one of the central questions in cellular communication. The symbiosis between the filamentous fungus Cladonia grayi and the single-celled green alga Astero ... Full text Cite

Sizing the fungal and algal genomes of the lichen Cladonia grayi through quantitative PCR

Journal Article Symbiosis · October 16, 2009 Using a method based on quantitative PCR, we determined that the nuclear genome sizes for the mycobiont and photobiont of the lichen Cladonia grayi are 28.6 Mb and 106.7 Mb, respectively. This is the first genome size determination for lichens, and suggest ... Full text Cite

Light might regulate divergently depside and depsidone accumulation in the lichen Parmotrema hypotropum by affecting thallus temperature and water potential.

Journal Article Mycologia · July 2008 Depsides and depsidones are the most common secondary products uniquely produced in lichens by the fungal symbiont, and they accumulate on the outer surface of its hyphae. Their biological roles are subject to debate. Quantitatively the compounds typical o ... Full text Cite

Symbiosis and DNA methylation in the Cladonia lichen fungus

Journal Article Symbiosis · January 1, 1999 In eukaryotes, the modification of DNA by addition or removal of specific methyl groups is thought to affect gene activity and differentiation. We began to investigate the relationship between DNA methylation and differentiation in lichens, organisms in wh ... Cite

A rapid and inexpensive method for the purification of DNA from lichens and their symbionts

Journal Article The Lichenologist · January 1, 1995 A simple DNA extraction method is described, applicable to many different kinds of lichens. The method involves the use of the detergents DTAB and CTAB and yields DNA that can be directly amplified with the polymerase chain reaction or digested with restri ... Full text Cite

Lichen Lore

Journal Article BioScience · March 1994 Full text Cite

Induction of a complete secondary-product pathway in a cultured lichen fungus

Journal Article Experimental Mycology · January 1, 1992 Experimental studies on the secondary metabolism characteristic of lichens have been impeded by the slow growth of the fungi and by the inconsistent results of many attempts to induce the pathways in the fungi isolated from their photosynthetic partners. I ... Full text Cite

Lichen chimeras: DNA analysis suggests that one fungus forms two morphotypes

Journal Article Experimental Mycology · January 1, 1991 In most lichens, the symbiosis between one fungus (mycobiont) and one photosynthetic partner (photobiont) results in a uniform thallus whose morphology is distinctive for each combination of symbionts. In some lichens, two morphologically different thalli, ... Full text Cite

Biolistic nuclear transformation of Saccharomyces cerevisiae and other fungi.

Journal Article Current genetics · February 1990 Tungsten microprojectiles coated with nucleic acid and accelerated to velocities of approximately 500 m/s, can penetrate living cells and tissues with consequent expression of the introduced genes (Klein et al. 1987). Saccharomyces cerevisiae is used here ... Full text Cite

A note on RNA polymerase phylogeny.

Journal Article Journal of theoretical biology · March 1988 Full text Cite

Structure and evolution of prokaryotic and eukaryotic RNA polymerases: a model.

Journal Article Journal of theoretical biology · August 1987 A comparative overview of the subunit taxonomy and sequences of eukaryotic and prokaryotic RNA polymerases indicates the presence of a core structure conserved between both sets of enzymes. The differentiation between prokaryotic and eukaryotic polymerases ... Full text Cite

Structural studies on Neurospora RNA polymerases and associated proteins.

Journal Article The Journal of biological chemistry · December 1985 Extensive structural homology between the three nuclear RNA polymerases of Neurospora crassa has been observed by peptide and immunological analyses. Within each polymerase, we found structural similarity between subunits in the 65- to 75-kDa range and one ... Full text Cite

Depside and Depsidone Synthesis in Lichenized Fungi Comes into Focus through a Genome-Wide Comparison of the Olivetoric Acid and Physodic Acid Chemotypes of Pseudevernia furfuracea.

Journal Article Biomolecules · October 2021 Primary biosynthetic enzymes involved in the synthesis of lichen polyphenolic compounds depsides and depsidones are non-reducing polyketide synthases (NR-PKSs), and cytochrome P450s. However, for most depsides and depsidones the corresponding PKSs are unkn ... Full text Cite

Modeling in yeast how rDNA introns slow growth and increase desiccation tolerance in lichens.

Journal Article G3 (Bethesda, Md.) · October 2021 We connect ribosome biogenesis to desiccation tolerance in lichens, widespread symbioses between specialized fungi (mycobionts) and unicellular phototrophs. We test whether the introns present in the nuclear ribosomal DNA of lichen mycobionts contribute to ... Full text Cite

The lichen symbiosis re-viewed through the genomes of Cladonia grayi and its algal partner Asterochloris glomerata.

Journal Article BMC Genomics · July 23, 2019 BACKGROUND: Lichens, encompassing 20,000 known species, are symbioses between specialized fungi (mycobionts), mostly ascomycetes, and unicellular green algae or cyanobacteria (photobionts). Here we describe the first parallel genomic analysis of the mycobi ... Full text Link to item Cite

A method for simultaneous targeted mutagenesis of all nuclear rDNA repeats in Saccharomyces cerevisiae using CRISPR-Cas9

Journal Article bioRxiv · March 3, 2019 Saccharomyces cerevisiae has been the prime model to study the assembly and functionality of eukaryotic ribosomes. Within that vast landscape, the specific problem of mutagenizing all 150 nuclear rRNA genes was bypassed using strains whose chromosomal copi ... Full text Link to item Cite

Insights from the first putative biosynthetic gene cluster for a lichen depside and depsidone.

Journal Article Mycologia · July 2011 The genes for polyketide synthases (PKSs), enzymes that assemble the carbon backbones of many secondary metabolites, often cluster with other secondary pathway genes. We describe here the first lichen PKS cluster likely to be implicated in the biosynthesis ... Full text Cite

Fungal and algal gene expression in early developmental stages of lichen-symbiosis.

Journal Article Mycologia · March 2011 How plants and microbes recognize each other and interact to form long-lasting relationships remains one of the central questions in cellular communication. The symbiosis between the filamentous fungus Cladonia grayi and the single-celled green alga Astero ... Full text Cite

Sizing the fungal and algal genomes of the lichen Cladonia grayi through quantitative PCR

Journal Article Symbiosis · October 16, 2009 Using a method based on quantitative PCR, we determined that the nuclear genome sizes for the mycobiont and photobiont of the lichen Cladonia grayi are 28.6 Mb and 106.7 Mb, respectively. This is the first genome size determination for lichens, and suggest ... Full text Cite

Light might regulate divergently depside and depsidone accumulation in the lichen Parmotrema hypotropum by affecting thallus temperature and water potential.

Journal Article Mycologia · July 2008 Depsides and depsidones are the most common secondary products uniquely produced in lichens by the fungal symbiont, and they accumulate on the outer surface of its hyphae. Their biological roles are subject to debate. Quantitatively the compounds typical o ... Full text Cite

Symbiosis and DNA methylation in the Cladonia lichen fungus

Journal Article Symbiosis · January 1, 1999 In eukaryotes, the modification of DNA by addition or removal of specific methyl groups is thought to affect gene activity and differentiation. We began to investigate the relationship between DNA methylation and differentiation in lichens, organisms in wh ... Cite

A rapid and inexpensive method for the purification of DNA from lichens and their symbionts

Journal Article The Lichenologist · January 1, 1995 A simple DNA extraction method is described, applicable to many different kinds of lichens. The method involves the use of the detergents DTAB and CTAB and yields DNA that can be directly amplified with the polymerase chain reaction or digested with restri ... Full text Cite

Lichen Lore

Journal Article BioScience · March 1994 Full text Cite

Induction of a complete secondary-product pathway in a cultured lichen fungus

Journal Article Experimental Mycology · January 1, 1992 Experimental studies on the secondary metabolism characteristic of lichens have been impeded by the slow growth of the fungi and by the inconsistent results of many attempts to induce the pathways in the fungi isolated from their photosynthetic partners. I ... Full text Cite

Lichen chimeras: DNA analysis suggests that one fungus forms two morphotypes

Journal Article Experimental Mycology · January 1, 1991 In most lichens, the symbiosis between one fungus (mycobiont) and one photosynthetic partner (photobiont) results in a uniform thallus whose morphology is distinctive for each combination of symbionts. In some lichens, two morphologically different thalli, ... Full text Cite

Biolistic nuclear transformation of Saccharomyces cerevisiae and other fungi.

Journal Article Current genetics · February 1990 Tungsten microprojectiles coated with nucleic acid and accelerated to velocities of approximately 500 m/s, can penetrate living cells and tissues with consequent expression of the introduced genes (Klein et al. 1987). Saccharomyces cerevisiae is used here ... Full text Cite

A note on RNA polymerase phylogeny.

Journal Article Journal of theoretical biology · March 1988 Full text Cite

Structure and evolution of prokaryotic and eukaryotic RNA polymerases: a model.

Journal Article Journal of theoretical biology · August 1987 A comparative overview of the subunit taxonomy and sequences of eukaryotic and prokaryotic RNA polymerases indicates the presence of a core structure conserved between both sets of enzymes. The differentiation between prokaryotic and eukaryotic polymerases ... Full text Cite

Structural studies on Neurospora RNA polymerases and associated proteins.

Journal Article The Journal of biological chemistry · December 1985 Extensive structural homology between the three nuclear RNA polymerases of Neurospora crassa has been observed by peptide and immunological analyses. Within each polymerase, we found structural similarity between subunits in the 65- to 75-kDa range and one ... Full text Cite

Purification of the three nuclear RNA polymerases from Neurospora crassa.

Journal Article The Journal of biological chemistry · December 1985 Nuclear RNA polymerases I, II, and III from Neurospora crassa have been purified 3,000-, 1,500-, and 10,000-fold, respectively, by a procedure that minimizes proteolysis of the 220-kDa subunit of polymerase II. The Neurospora enzymes resemble, in polypepti ... Full text Cite

Effect of alpha-isopropylmalate on the synthesis of RNA and protein in Neurospora.

Journal Article Molecular & general genetics : MGG · January 1985 The leu-3/alpha-IPM (alpha-isopropylmalate) regulatory system, previously shown to control several genes of leucine, isoleucine, valine, and histidine biosynthesis, appears likely to be involved also in the regulation of overall RNA and protein synthesis i ... Full text Cite

PURIFICATION OF THE 3 NUCLEAR-RNA POLYMERASES FROM NEUROSPORA-CRASSA

Journal Article JOURNAL OF BIOLOGICAL CHEMISTRY · January 1, 1985 Link to item Cite

STRUCTURAL STUDIES ON NEUROSPORA RNA-POLYMERASES AND ASSOCIATED PROTEINS

Journal Article JOURNAL OF BIOLOGICAL CHEMISTRY · January 1, 1985 Link to item Cite