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David Stuart Millington

Professor Emeritus of Pediatrics
Pediatrics, Medical Genetics
Box 3028 Med Ctr, Durham, NC 27710
801-6 Capitola Drive, Box 103856, Durham, NC 27713

Overview


Development of new mass spectrometric methods for the analysis of disease-specific metabolites in human physiological fluids for application to the diagnosis of inborn errors of metabolism. The development of automated methods based on tandem mass spectrometry for the analysis of these metabolites in blood spots and urine from neonates, with a view to revolutionizing newborn screening methodology.
Development and application of mass spectrometry based biomarker assays for monitoring treatment and diagnosis of lysosomal storage diseases (LSDs).
Development and application of methods for analysis of drugs used in human clinical trials.
Application of "lab-on-a-chip" technology, based on digital microfluidics, to enzymatic and DNA- based assays used in newborn screening and at the point of care.
Development and application of new methods for studing clucose metabolism both in vivo and in vitro using stable isotope labeling with tandem mass spectrometry.
Development and application of methods for the analysis of urine markers of oxidative stress, using tandem mass spectrometry.

Current Appointments & Affiliations


Professor Emeritus of Pediatrics · 2015 - Present Pediatrics, Medical Genetics, Pediatrics

Recent Publications


Quantification of Glycosaminoglycans in Urine by Isotope Dilution Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry.

Journal Article Curr Protoc · February 2025 Mucopolysaccharidoses (MPSs) are complex lysosomal diseases that result in the accumulation of glycosaminoglycans (GAGs) in urine, blood, and tissues. Lysosomal enzymes responsible for GAG degradation are defective in MPSs. GAGs including chondroitin sulfa ... Full text Link to item Cite

How mass spectrometry revolutionized newborn screening.

Journal Article J Mass Spectrom Adv Clin Lab · April 2024 This article offers a personal account of a remarkable journey spanning over 30 years of applied mass spectrometry in a clinical setting. It begins with the author's inspiration from a clinician's story of rescuing a child from near death with a revolution ... Full text Link to item Cite

A complementary approach: Metabolomics

Chapter · January 1, 2024 This chapter presents a brief overview of metabolomics, the history of its development, principles, and applications. The differences between targeted and untargeted metabolomics are explained, and the advantages and limitations of both approaches are disc ... Full text Cite
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Recent Grants


Gender and APOE genotype interact to alter immune regulated metabolism in AD

ResearchCo Investigator · Awarded by National Institutes of Health · 2019 - 2024

Gastrin-Releasing Peptide and Bronchopulmonary Dysplasia

ResearchSignificant Contributor · Awarded by National Institutes of Health · 2011 - 2018

Dietary therapy in mitochondrial trifunctional protein deficiency

ResearchConsultant · Awarded by Ultragenyx Pharmaceutical · 2014 - 2015

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Education, Training & Certifications


University of Liverpool (United Kingdom) · 1969 Ph.D.