Kenneth N. Kreuzer
Professor Emeritus of Biochemistry
Our interests are in the molecular mechanisms of DNA replication, recombination and repair, using bacteriophage T4 and Escherichia coli
as model systems. In one project, we are investigating the molecular mechanism of phage T4 origin-dependent DNA replication from RNA-DNA hybrids (R loops). The process of “constitutive stable DNA replication” in E. coli
has also been proposed to occur via R-loop intermediates, and we are attempting to isolate and analyze the origins for this mode of DNA synthesis.
DNA replication forks often fail during the elongation process, particularly after treatment with agents that cause template DNA damage. One severe form of failure is replication fork breakage, in which one arm of the fork is broken from the branch. Homologous recombination plays a critical role in repairing and restarting these broken replication forks in all cells, and disturbances in this process can lead to genome instability. We have studied double-strand-break directed replication in phage T4, which provides an excellent model system for this process of recombination-dependent DNA replication. Our recent studies on the phage T4 MR (Mre11-Rad50) complex provide evidence that the nuclease activity of the protein is important in this process, and also that the MR complex plays a role in coordinating two broken ends during double-strand break repair.
We are interested in the mechanism of cytotoxicity of inhibitors of type II DNA topoisomerases, and we have obtained evidence that cytotoxicity may involve perturbation of replication fork behavior. This group includes important anticancer agents, such as doxorubicin and etoposide, and the fluoroquinolone group of antibacterial agents, such as ciprofloxacin. All of these inhibitors stabilize a reaction intermediate, called the cleavage complex, in which the topoisomerase is covalently attached to cleaved DNA. We have shown that drug-stabilized cleavage complexes block replication forks in vivo both in phage T4 infections, which are sensitive to the anticancer agents, and in uninfected E. coli cells, which are sensitive to the fluoroquinolones. Furthermore, we found that these blocked replication forks are prone to breakage, in at least some cases by recombination nucleases that cut branched DNA. We propose that this “collateral damage” from topoisomerase inhibitors constitutes cytotoxic damage, and may also be involved in genetic rearrangements induced during anticancer chemotherapy. Recently, we have extended our studies of anticancer drugs to the nucleotide analog aza-cytosine, which traps covalent methylase-DNA complexes. As with the topoisomerase inhibitors, this drug blocks replication forks at the sites of covalent protein-DNA complexes.
Much interest has recently focused on the physiological roles of DNA helicases, particularly with the discovery of a helicase defect in Bloom’s and Werner’s syndromes in humans, which cause predisposition to cancer. We have shown that the phage T4 UvsW protein is a helicase that unwinds the origin R loops, and provided evidence that this protein is thereby a negative regulator of origin usage at late times of infection. The UvsW protein is also important in phage recombination, DNA repair, and mutation. We are collaborating with Dr Stephen White (St. Jude Childrens Research Hospital) to analyze the structure and function of the UvsW helicase and to identify its molecular role in recombination and repair.
DNA replication forks often fail during the elongation process, particularly after treatment with agents that cause template DNA damage. One severe form of failure is replication fork breakage, in which one arm of the fork is broken from the branch. Homologous recombination plays a critical role in repairing and restarting these broken replication forks in all cells, and disturbances in this process can lead to genome instability. We have studied double-strand-break directed replication in phage T4, which provides an excellent model system for this process of recombination-dependent DNA replication. Our recent studies on the phage T4 MR (Mre11-Rad50) complex provide evidence that the nuclease activity of the protein is important in this process, and also that the MR complex plays a role in coordinating two broken ends during double-strand break repair.
We are interested in the mechanism of cytotoxicity of inhibitors of type II DNA topoisomerases, and we have obtained evidence that cytotoxicity may involve perturbation of replication fork behavior. This group includes important anticancer agents, such as doxorubicin and etoposide, and the fluoroquinolone group of antibacterial agents, such as ciprofloxacin. All of these inhibitors stabilize a reaction intermediate, called the cleavage complex, in which the topoisomerase is covalently attached to cleaved DNA. We have shown that drug-stabilized cleavage complexes block replication forks in vivo both in phage T4 infections, which are sensitive to the anticancer agents, and in uninfected E. coli cells, which are sensitive to the fluoroquinolones. Furthermore, we found that these blocked replication forks are prone to breakage, in at least some cases by recombination nucleases that cut branched DNA. We propose that this “collateral damage” from topoisomerase inhibitors constitutes cytotoxic damage, and may also be involved in genetic rearrangements induced during anticancer chemotherapy. Recently, we have extended our studies of anticancer drugs to the nucleotide analog aza-cytosine, which traps covalent methylase-DNA complexes. As with the topoisomerase inhibitors, this drug blocks replication forks at the sites of covalent protein-DNA complexes.
Much interest has recently focused on the physiological roles of DNA helicases, particularly with the discovery of a helicase defect in Bloom’s and Werner’s syndromes in humans, which cause predisposition to cancer. We have shown that the phage T4 UvsW protein is a helicase that unwinds the origin R loops, and provided evidence that this protein is thereby a negative regulator of origin usage at late times of infection. The UvsW protein is also important in phage recombination, DNA repair, and mutation. We are collaborating with Dr Stephen White (St. Jude Childrens Research Hospital) to analyze the structure and function of the UvsW helicase and to identify its molecular role in recombination and repair.
Current Appointments & Affiliations
- Professor Emeritus of Biochemistry, Biochemistry, Basic Science Departments 2015
Contact Information
- 155A Nanaline Duke, Durham, NC 27710
- Duke Box 3711, Durham, NC 27710
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kenneth.kreuzer@duke.edu
(919) 684-6466
- Background
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Education, Training, & Certifications
- Ph.D., University of Chicago 1978
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Duke Appointment History
- Member of the Duke Cancer Institute, Duke Cancer Institute, Institutes and Centers 1984 - 2015
- Professor of Biochemistry, Biochemistry, Basic Science Departments 2001 - 2015
- Interim Chair, Department of Biochemistry, Biochemistry, Basic Science Departments 2007 - 2010
- Professor of Microbiology, Molecular Genetics and Microbiology, Basic Science Departments 2001 - 2002
- Professor of Microbiology, Molecular Genetics and Microbiology, Basic Science Departments 2001
- Associate Professor of Microbiology, Molecular Genetics and Microbiology, Basic Science Departments 1991 - 2001
- Recognition
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Awards & Honors
- Research
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Selected Grants
- Increasing Diversity and Student Engagement in the BioSciences awarded by Merck Foundation 2014 - 2015
- Processing and consequences of DNA-protein crosslinks in E. coli awarded by National Institutes of Health 2002 - 2013
- Duke PREP: Minority Recruitment into Biomedical Sciences awarded by National Institutes of Health 2003 - 2008
- Initiation of DNA Replication in the Phage T4 System awarded by National Institutes of Health 1985 - 2006
- Antitumor Agents And The Bacteriophage T4 Topoisomerase awarded by National Institutes of Health 1993 - 2003
- A Persistent Rna-Dna Hybrid At A T4 Replication Origin awarded by National Institutes of Health 1998 - 2000
- Initiation of T4 Replication - Study of an RNA/DNA Hybrid awarded by National Institutes of Health 1997 - 1999
- Initiation Of T4 Replication: Study Of An Rna-Dna Hybrid awarded by National Institutes of Health 1997 - 1999
- Initiation Of Dna Replication In The Phage T4 System awarded by National Institutes of Health 1985 - 1999
- Cancer Center Core Support Grant awarded by National Institutes of Health 1976 - 1998
- Comprehensive Cancer Center Core Support Grant awarded by National Institutes of Health 1976 - 1998
- Comprehensive Cancer Center Core Support Grant awarded by National Institutes of Health 1976 - 1998
- Antitumor Agents And The Bacteriophage T4 Topoisomerase awarded by National Institutes of Health 1993 - 1998
- Initiation Of Dna Replication In The Phage T4 Systems awarded by National Institutes of Health 1990 - 1993
- Publications & Artistic Works
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Selected Publications
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Academic Articles
- Barfoot, T, Herdendorf, TJ, Behning, BR, Stohr, BA, Gao, Y, Kreuzer, KN, and Nelson, SW. "Functional Analysis of the Bacteriophage T4 Rad50 Homolog (gp46) Coiled-coil Domain." The Journal of Biological Chemistry 290, no. 39 (September 2015): 23905-23915. Full Text
- Whatley, Z, and Kreuzer, KN. "Mutations that Separate the Functions of the Proofreading Subunit of the Escherichia coli Replicase." G3 (Bethesda, Md.) 5, no. 6 (April 15, 2015): 1301-1311. Full Text
- Krasich, R, Wu, SY, Kuo, HK, and Kreuzer, KN. "Functions that protect Escherichia coli from DNA-protein crosslinks." DNA repair 28 (April 2015): 48-59. Full Text
- Henderson, ML, and Kreuzer, KN. "Functions that Protect Escherichia coli from Tightly Bound DNA-Protein Complexes Created by Mutant EcoRII Methyltransferase." PloS one 10, no. 5 (January 2015): e0128092-. Full Text
- Maduike, NZ, Tehranchi, AK, Wang, JD, and Kreuzer, KN. "Replication of the Escherichia coli chromosome in RNase HI-deficient cells: Multiple initiation regions and fork dynamics." Molecular Microbiology 91, no. 1 (January 1, 2014): 39-56. Full Text
- Maduike, NZ, Tehranchi, AK, Wang, JD, and Kreuzer, KN. "Replication of the Escherichia coli chromosome in RNase HI-deficient cells: multiple initiation regions and fork dynamics." Molecular Microbiology 91, no. 1 (January 2014): 39-56. Full Text
- Almond, JR, Stohr, BA, Panigrahi, AK, Albrecht, DW, Nelson, SW, and Kreuzer, KN. "Coordination and processing of DNA ends during double-strand break repair: the role of the bacteriophage T4 Mre11/Rad50 (MR) complex." Genetics 195, no. 3 (November 2013): 739-755. Full Text Link to Item
- Kreuzer, KN. "DNA damage responses in prokaryotes: regulating gene expression, modulating growth patterns, and manipulating replication forks." Cold Spring Harbor perspectives in biology 5, no. 11 (November 1, 2013). (Review)
- Kreuzer, KN. "DNA damage responses in prokaryotes: regulating gene expression, modulating growth patterns, and manipulating replication forks." Cold Spring Harbor Perspectives in Biology 5, no. 11 (November 2013): a012674-null. (Review) Full Text
- He, X, Byrd, AK, Yun, M-K, Pemble, CW, Harrison, D, Yeruva, L, Dahl, C, Kreuzer, KN, Raney, KD, and White, SW. "The T4 phage SF1B helicase Dda is structurally optimized to perform DNA strand separation." Structure (London, England : 1993) 20, no. 7 (July 2012): 1189-1200. Full Text
- Gajewski, S, Webb, MR, Galkin, V, Egelman, EH, Kreuzer, KN, and White, SW. "Crystal structure of the phage T4 recombinase UvsX and its functional interaction with the T4 SF2 helicase UvsW." Journal of Molecular Biology 405, no. 1 (January 2011): 65-76. Full Text
- Kuo, HK, Krasich, R, Bhagwat, AS, and Kreuzer, KN. "Importance of the tmRNA system for cell survival when transcription is blocked by DNA-protein cross-links." Mol Microbiol 78, no. 3 (November 2010): 686-700. Full Text Link to Item
- Kreuzer, KN, and Brister, JR. "Initiation of bacteriophage T4 DNA replication and replication fork dynamics: a review in the Virology Journal series on bacteriophage T4 and its relatives." Virology Journal 7 (January 2010): 358-null. (Review) Full Text
- Long, DT, and Kreuzer, KN. "Fork regression is an active helicase-driven pathway in bacteriophage T4." Embo Reports 10, no. 4 (April 2009): 394-399. Full Text
- Pohlhaus, JR, Long, DT, O'Reilly, E, and Kreuzer, KN. "The epsilon subunit of DNA polymerase III Is involved in the nalidixic acid-induced SOS response in Escherichia coli." Journal of Bacteriology 190, no. 15 (August 2008): 5239-5247. Full Text
- Long, DT, and Kreuzer, KN. "Regression supports two mechanisms of fork processing in phage T4." Proc Natl Acad Sci U S A 105, no. 19 (May 13, 2008): 6852-6857. Full Text Link to Item
- Webb, MR, Plank, JL, Long, DT, Hsieh, T-S, and Kreuzer, KN. "The phage T4 protein UvsW drives Holliday junction branch migration." The Journal of Biological Chemistry 282, no. 47 (November 2007): 34401-34411. Full Text
- Kuo, HK, Griffith, JD, and Kreuzer, KN. "5-Azacytidine induced methyltransferase-DNA adducts block DNA replication in vivo." Cancer Research 67, no. 17 (September 2007): 8248-8254. Full Text
- Pohlhaus, JR, and Kreuzer, KN. "Formation and processing of stalled replication forks--utility of two-dimensional agarose gels." Methods Enzymol 409 (2006): 477-493. Full Text Link to Item
- Newmark, KG, O'Reilly, EK, Pohlhaus, JR, and Kreuzer, KN. "Genetic analysis of the requirements for SOS induction by nalidixic acid in Escherichia coli." Gene 356 (August 2005): 69-76. Full Text
- Dudas, KC, and Kreuzer, KN. "Bacteriophage T4 helicase loader protein gp59 functions as gatekeeper in origin-dependent replication in vivo." The Journal of Biological Chemistry 280, no. 22 (June 2005): 21561-21569. Full Text
- Pohlhaus, JR, and Kreuzer, KN. "Norfloxacin-induced DNA gyrase cleavage complexes block Escherichia coli replication forks, causing double-stranded breaks in vivo." Mol Microbiol 56, no. 6 (June 2005): 1416-1429. Full Text Link to Item
- Adhya, S, Black, L, Friedman, D, Hatfull, G, Kreuzer, K, Merril, C, Oppenheim, A, Rohwer, F, and Young, R. "2004 ASM Conference on the New Phage Biology: The 'Phage Summit'." Molecular Microbiology 55, no. 5 (2005): 1300-1314. Full Text
- Kreuzer, KN. "Interplay between DNA replication and recombination in prokaryotes." Annual Review of Microbiology 59 (January 2005): 43-67. Full Text
- O'Reilly, EK, and Kreuzer, KN. "Isolation of SOS constitutive mutants of Escherichia coli." Journal of Bacteriology 186, no. 21 (November 2004): 7149-7160. Full Text
- Sickmier, EA, Kreuzer, KN, and White, SW. "The crystal structure of the UvsW helicase from bacteriophage T4." Structure (London, England : 1993) 12, no. 4 (April 2004): 583-592. Full Text
- Hong, G, and Kreuzer, KN. "Endonuclease cleavage of blocked replication forks: An indirect pathway of DNA damage from antitumor drug-topoisomerase complexes." Proc Natl Acad Sci U S A 100, no. 9 (April 29, 2003): 5046-5051. Full Text Link to Item
- Stohr, BA, and Kreuzer, KN. "Coordination of DNA ends during double-strand-break repair in bacteriophage T4." Genetics 162, no. 3 (November 2002): 1019-1030.
- O'Reilly, EK, and Kreuzer, KN. "A unique type II topoisomerase mutant that is hypersensitive to a broad range of cleavage-inducing antitumor agents." Biochemistry 41, no. 25 (June 2002): 7989-7997. Full Text
- Klein, HL, and Kreuzer, KN. "Replication, recombination, and repair: going for the gold." Mol Cell 9, no. 3 (March 2002): 471-480. Link to Item
- George, JW, Stohr, BA, Tomso, DJ, and Kreuzer, KN. "The tight linkage between DNA replication and double-strand break repair in bacteriophage T4." Proceedings of the National Academy of Sciences of the United States of America 98, no. 15 (July 2001): 8290-8297. (Review) Full Text
- Jones, CE, Mueser, TC, Dudas, KC, Kreuzer, KN, and Nossal, NG. "Bacteriophage T4 gene 41 helicase and gene 59 helicase-loading protein: a versatile couple with roles in replication and recombination." Proceedings of the National Academy of Sciences of the United States of America 98, no. 15 (July 2001): 8312-8318. (Review) Full Text
- Stohr, BA, and Kreuzer, KN. "Repair of topoisomerase-mediated DNA damage in bacteriophage T4." Genetics 158, no. 1 (May 2001): 19-28. Link to Item
- Cicero, MP, Sharp, MM, Gross, CA, and Kreuzer, KN. "Substitutions in bacteriophage T4 AsiA and Escherichia coli sigma(70) that suppress T4 motA activation mutations." Journal of Bacteriology 183, no. 7 (April 2001): 2289-2297. Full Text
- Dudas, KC, and Kreuzer, KN. "UvsW protein regulates bacteriophage T4 origin-dependent replication by unwinding R-loops." Molecular and Cellular Biology 21, no. 8 (April 2001): 2706-2715. Full Text
- Doan, PL, Belanger, KG, and Kreuzer, KN. "Two types of recombination hotspots in bacteriophage T4: one requires DNA damage and a replication origin and the other does not." Genetics 157, no. 3 (March 2001): 1077-1087.
- Kreuzer, KN, Freudenreich, CH, and Pommier, Y. "Analysis of cleavage complexes using reactive inhibitor derivatives." Methods Mol Biol 95 (2001): 89-99. Link to Item
- Nossal, NG, Dudas, KC, and Kreuzer, KN. "Bacteriophage T4 proteins replicate plasmids with a preformed R loop at the T4 ori(uvsY) replication origin in vitro." Molecular Cell 7, no. 1 (January 2001): 31-41. Full Text
- Stohr, BA, and Kreuzer, KN. "Recognition and specific degradation of bacteriophage T4 mRNAs." Genetics 158, no. 1 (2001): 7-17.
- Tomso, DJ, and Kreuzer, KN. "Double-strand break repair in tandem repeats during bacteriophage T4 infection." Genetics 155, no. 4 (August 2000): 1493-1504. Link to Item
- Kreuzer, KN. "Recombination-dependent DNA replication in phage T4." Trends in Biochemical Sciences 25, no. 4 (April 2000): 165-173. (Review) Full Text
- Cox, MM, Goodman, MF, Kreuzer, KN, Sherratt, DJ, Sandler, SJ, and Marians, KJ. "The importance of repairing stalled replication forks." Nature 404, no. 6773 (March 2000): 37-41. Full Text
- Hong, G, and Kreuzer, KN. "An antitumor drug-induced topoisomerase cleavage complex blocks a bacteriophage T4 replication fork in vivo." Molecular and Cellular Biology 20, no. 2 (January 2000): 594-603. Full Text
- Kreuzer, KN, and Neece, SH. "Purification of the bacteriophage T4 type II DNA topoisomerase." Methods Mol Biol 94 (1999): 171-177. Full Text Link to Item
- Belanger, KG, and Kreuzer, KN. "Bacteriophage T4 initiates bidirectional DNA replication through a two-step process." Molecular Cell 2, no. 5 (November 1998): 693-701. Full Text
- Kreuzer, KN. "Bacteriophage T4, a model system for understanding the mechanism of type II topoisomerase inhibitors." Biochim Biophys Acta 1400, no. 1-3 (October 1, 1998): 339-347. (Review) Link to Item
- Cicero, MP, Alexander, KA, and Kreuzer, KN. "The MotA transcriptional activator of bacteriophage T4 binds to its specific DNA site as a monomer." Biochemistry 37, no. 14 (April 1998): 4977-4984. Full Text
- Freudenreich, CH, Chang, C, and Kreuzer, KN. "Mutations of the bacteriophage T4 type II DNA topoisomerase that alter sensitivity to antitumor agent 4'-(9-acridinylamino)methanesulfon-m-anisidide and an antibacterial quinolone." Cancer Res 58, no. 6 (March 15, 1998): 1260-1267. Link to Item
- Carles-Kinch, K, George, JW, and Kreuzer, KN. "Bacteriophage T4 UvsW protein is a helicase involved in recombination, repair and the regulation of DNA replication origins." The Embo Journal 16, no. 13 (July 1997): 4142-4151. Full Text
- Finnin, MS, Cicero, MP, Davies, C, Porter, SJ, White, SW, and Kreuzer, KN. "The activation domain of the MotA transcription factor from bacteriophage T4." The Embo Journal 16, no. 8 (April 1997): 1992-2003. Full Text
- Carles-Kinch, K, and Kreuzer, KN. "RNA-DNA hybrid formation at a bacteriophage T4 replication origin." Journal of Molecular Biology 266, no. 5 (March 1997): 915-926. Full Text
- George, JW, and Kreuzer, KN. "Repair of double-strand breaks in bacteriophage T4 by a mechanism that involves extensive DNA replication." Genetics 143, no. 4 (August 1996): 1507-1520. Link to Item
- Woodworth, DL, and Kreuzer, KN. "Bacteriophage T4 mutants hypersensitive to an antitumor agent that induces topoisomerase-DNA cleavage complexes." Genetics 143, no. 3 (July 1996): 1081-1090.
- Belanger, KG, Mirzayan, C, Kreuzer, HE, Alberts, BM, and Kreuzer, KN. "Two-dimensional gel analysis of rolling circle replication in the presence and absence of bacteriophage T4 primase." Nucleic Acids Research 24, no. 11 (June 1996): 2166-2175. Full Text
- Frothingham, R, Meeker-O'Connell, WA, Talbot, EA, George, JW, and Kreuzer, KN. "Identification, cloning, and expression of the Escherichia coli pyrazinamidase and nicotinamidase gene, pncA." Antimicrobial Agents and Chemotherapy 40, no. 6 (June 1996): 1426-1431. Full Text
- Neece, SH, Carles-Kinch, K, Tomso, DJ, and Kreuzer, KN. "Role of recombinational repair in sensitivity to an antitumour agent that inhibits bacteriophage T4 type II DNA topoisomerase." Molecular Microbiology 20, no. 6 (June 1996): 1145-1154. Full Text
- Kreuzer, KN, Saunders, M, Weislo, LJ, and Kreuzer, HW. "Recombination-dependent DNA replication stimulated by double-strand breaks in bacteriophage T4." J Bacteriol 177, no. 23 (December 1995): 6844-6853. Link to Item
- Howard, MT, Neece, SH, Matson, SW, and Kreuzer, KN. "Disruption of a topoisomerase-DNA cleavage complex by a DNA helicase." Proc Natl Acad Sci U S A 91, no. 25 (December 6, 1994): 12031-12035. Link to Item
- Kreuzer, HW, and Kreuzer, KN. "Integration of plasmids into the bacteriophage T4 genome." Genetics 138, no. 4 (December 1994): 983-992.
- Freudenreich, CH, and Kreuzer, KN. "Localization of an aminoacridine antitumor agent in a type II topoisomerase-DNA complex." Proceedings of the National Academy of Sciences of the United States of America 91, no. 23 (November 1994): 11007-11011. Full Text
- CARLESKINCH, K, and KREUZER, KN. "ANALYSIS OF ORIGIN-DEPENDENT DNA-REPLICATION INITIATION IN BACTERIOPHAGE-T4." JOURNAL OF CELLULAR BIOCHEMISTRY (February 13, 1994): 131-131. Link to Item
- HOWARD, MT, NEECE, SH, MATSON, SW, and KREUZER, KN. "A DNA HELICASE INDUCES TOPOISOMERASE II-ASSOCIATED DNA BREAKS." JOURNAL OF CELLULAR BIOCHEMISTRY (February 13, 1994): 152-152. Link to Item
- Freudenreich, CH, and Kreuzer, KN. "Differentially labeled mutant oligonucleotides for analysis of protein-DNA interactions." Biotechniques 16, no. 1 (January 1994): 104-108. Link to Item
- Kreuzer, KN. "A bacteriophage model system for studying topoisomerase inhibitors." Adv Pharmacol 29B (1994): 171-186. (Review) Link to Item
- Finnin, MS, Hoffman, DW, Kreuzer, KN, Porter, SJ, Schmidt, RP, and White, SW. "The MotA protein from bacteriophage T4 contains two domains. Preliminary structural analysis by X-ray diffraction and nuclear magnetic resonance." J Mol Biol 232, no. 1 (July 5, 1993): 301-304. Full Text Link to Item
- Freudenreich, CH, and Kreuzer, KN. "Mutational analysis of a type II topoisomerase cleavage site: distinct requirements for enzyme and inhibitors." The Embo Journal 12, no. 5 (May 1993): 2085-2097. Full Text
- WOODWORTH, DL, and KREUZER, KN. "THE ISOLATION OF PHAGE-T4 MUTANTS ULTRASENSITIVE TO M-AMSA - DEFINING A PATHWAY FOR REPAIR OF PROTEIN-DNA CROSS-LINKS." JOURNAL OF CELLULAR BIOCHEMISTRY (March 29, 1993): 308-308. Link to Item
- FREUDENREICH, CH, and KREUZER, KN. "MUTATIONAL ANALYSIS OF A TYPE-II TOPOISOMERASE CLEAVAGE SITE - BASE PREFERENCES FOR THE ENZYME AND FOR INHIBITORS." JOURNAL OF CELLULAR BIOCHEMISTRY (February 8, 1993): 170-170. Link to Item
- Engman, HW, and Kreuzer, KN. "Deletion of the essential gene 24 from the bacteriophage T4 genome." Gene 123, no. 1 (January 1993): 69-74. Full Text
- Benson, KH, and Kreuzer, KN. "Plasmid models for bacteriophage T4 DNA replication: requirements for fork proteins." J Virol 66, no. 12 (December 1992): 6960-6968. Link to Item
- Benson, KH, and Kreuzer, KN. "Role of MotA transcription factor in bacteriophage T4 DNA replication." Journal of Molecular Biology 228, no. 1 (November 1992): 88-100. Full Text
- Schmidt, RP, and Kreuzer, KN. "Purified MotA protein binds the -30 region of a bacteriophage T4 middle-mode promoter and activates transcription in vitro." J Biol Chem 267, no. 16 (June 5, 1992): 11399-11407. Link to Item
- Woodworth, DL, and Kreuzer, KN. "A system of transposon mutagenesis for bacteriophage T4." Molecular Microbiology 6, no. 10 (May 1992): 1289-1296. Full Text
- Yap, WY, and Kreuzer, KN. "Recombination hotspots in bacteriophage T4 are dependent on replication origins." Proceedings of the National Academy of Sciences of the United States of America 88, no. 14 (July 1991): 6043-6047. Full Text
- Masurekar, M, Kreuzer, KN, and Ripley, LS. "The specificity of topoisomerase-mediated DNA cleavage defines acridine-induced frameshift specificity within a hotspot in bacteriophage T4." Genetics 127, no. 3 (March 1991): 453-462. Link to Item
- Masurekar, M, Kreuzer, KN, and Ripley, LS. "Erratum: ''The specificity of topoisomerase-mediated DNA cleavage defines acridine-induced frame-shift specificity within a hotspot in bacteriophage T4,'' (GENETICS 127: 453-462; March, 1991)." Genetics 128, no. 1 (1991): 193--.
- Huff, AC, and Kreuzer, KN. "Evidence for a common mechanism of action for antitumor and antibacterial agents that inhibit type II DNA topoisomerases." J Biol Chem 265, no. 33 (November 25, 1990): 20496-20505. Link to Item
- Derr, LK, and Kreuzer, KN. "Expression and function of the uvsW gene of bacteriophage T4." Journal of Molecular Biology 214, no. 3 (August 1990): 643-656. Full Text
- Huff, AC, Ward, RE, and Kreuzer, KN. "Mutational alteration of the breakage/resealing subunit of bacteriophage T4 DNA topoisomerase confers resistance to antitumor agent m-AMSA." Mol Gen Genet 221, no. 1 (March 1990): 27-32. Link to Item
- Huff, AC, Leatherwood, JK, and Kreuzer, KN. "Bacteriophage T4 DNA topoisomerase is the target of antitumor agent 4'-(9-acridinylamino)methanesulfon-m-anisidide (m-AMSA) in T4-infected Escherichia coli." Proc Natl Acad Sci U S A 86, no. 4 (February 1989): 1307-1311. Link to Item
- Kreuzer, KN. "DNA topoisomerases as potential targets of antiviral action." Pharmacol Ther 43, no. 3 (1989): 377-395. (Review) Link to Item
- Menkens, AE, and Kreuzer, KN. "Deletion analysis of bacteriophage T4 tertiary origins. A promoter sequence is required for a rifampicin-resistant replication origin." J Biol Chem 263, no. 23 (August 15, 1988): 11358-11365. Link to Item
- Kreuzer, KN, Engman, HW, and Yap, WY. "Tertiary initiation of replication in bacteriophage T4. Deletion of the overlapping uvsY promoter/replication origin from the phage genome." The Journal of Biological Chemistry 263, no. 23 (August 1988): 11348-11357.
- Kreuzer, KN, Yap, WY, Menkens, AE, and Engman, HW. "Recombination-dependent replication of plasmids during bacteriophage T4 infection." The Journal of Biological Chemistry 263, no. 23 (August 1988): 11366-11373.
- Selick, HE, Kreuzer, KN, and Alberts, BM. "The bacteriophage T4 insertion/substitution vector system. A method for introducing site-specific mutations into the virus chromosome." The Journal of Biological Chemistry 263, no. 23 (August 1988): 11336-11347.
- Ripley, LS, Dubins, JS, deBoer, JG, DeMarini, DM, Bogerd, AM, and Kreuzer, KN. "Hotspot sites for acridine-induced frameshift mutations in bacteriophage T4 correspond to sites of action of the T4 type II topoisomerase." J Mol Biol 200, no. 4 (April 20, 1988): 665-680. Link to Item
- Kreuzer, KN, and Alberts, BM. "Characterization of a defective phage system for the analysis of bacteriophage T4 DNA replication origins." J Mol Biol 188, no. 2 (March 20, 1986): 185-198. Link to Item
- KREUZER, KN, BENSON, KH, BOGERD, AM, MENKENS, AE, RINGLEY, RA, and YAP, WY. "PHAGE-T4 REPLICATION ORIGINS THAT COINCIDE WITH RECOMBINATION HOTSPOTS." JOURNAL OF CELLULAR BIOCHEMISTRY (1986): 178-178. Link to Item
- MENKENS, AE, and KREUZER, KN. "SEQUENCE-ANALYSIS OF PHAGE-T4 TERTIARY ORIGINS." JOURNAL OF CELLULAR BIOCHEMISTRY (1986): 182-182. Link to Item
- Kreuzer, KN, and Alberts, BM. "A defective phage system reveals bacteriophage T4 replication origins that coincide with recombination hot spots." Proc Natl Acad Sci U S A 82, no. 10 (May 1985): 3345-3349. Link to Item
- Kreuzer, KN, and Alberts, BM. "Site-specific recognition of bacteriophage T4 DNA by T4 type II DNA topoisomerase and Escherichia coli DNA gyrase." J Biol Chem 259, no. 8 (April 25, 1984): 5339-5346. Link to Item
- Kreuzer, KN. "Recognition of single-stranded DNA by the bacteriophage T4-induced type II topoisomerase." J Biol Chem 259, no. 8 (April 25, 1984): 5347-5354. Link to Item
- Alberts, BM, Barry, J, Bedinger, P, Formosa, T, Jongeneel, CV, and Kreuzer, KN. "Studies on DNA replication in the bacteriophage T4 in vitro system." Cold Spring Harbor Symposia on Quantitative Biology 47 Pt 2 (January 1983): 655-668. Full Text
- KREUZER, KN, and ALBERTS, B. "SPECIFIC RECOGNITION OF PHAGE-T4 DNA BY THE T4-INDUCED TYPE-II DNA TOPOISOMERASE." FEDERATION PROCEEDINGS 42, no. 7 (1983): 2087-2087. Link to Item
- Kreuzer, KN, and Jongeneel, CV. "Escherichia coli phage T4 topoisomerase." Methods Enzymol 100 (1983): 144-160. Link to Item
- ALBERTS, BM, BARRY, J, BEDINGER, P, FORMOSA, T, JONGENEEL, CV, and KREUZER, KN. "STUDIES ON DNA-REPLICATION IN THE BACTERIOPHAGE-T4 INVITRO SYSTEM." ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 184, no. SEP (1982): 71-&. Link to Item
- Kreuzer, KN, and Cozzarelli, NR. "Formation and resolution of DNA catenanes by DNA gyrase." Cell 20, no. 1 (May 1980): 245-254. Link to Item
- COZZARELLI, NR, BROWN, PO, MORRISON, A, KREUZER, KN, OTTER, R, and KRASNOW, M. "SHAPING OF DNA BY DNA GYRASE." ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 180, no. AUG (1980): 156-BIOL. Link to Item
- Kreuzer, KN, and Cozzarelli, NR. "Escherichia coli mutants thermosensitive for deoxyribonucleic acid gyrase subunit A: effects on deoxyribonucleic acid replication, transcription, and bacteriophage growth." J Bacteriol 140, no. 2 (November 1979): 424-435. Link to Item
- Peebles, CL, Higgins, NP, Kreuzer, KN, Morrison, A, Brown, PO, Sugino, A, and Cozzarelli, NR. "Structure and activities of Escherichia coli DNA gyrase." Cold Spring Harb Symp Quant Biol 43 Pt 1 (1979): 41-52. Link to Item
- Kreuzer, KN, McEntee, K, Geballe, AP, and Cozzarelli, NR. "Lambda transducing phages for the nalA gene of Escherichia coli and conditional lethal nalA mutations." Mol Gen Genet 167, no. 2 (November 29, 1978): 129-137. Link to Item
- HIGGINS, NP, KREUZER, KN, MORRISON, A, SUGINO, A, PEEBLES, CL, BROWN, P, and COZZARELLI, NR. "DNA GYRASE - TARGET FOR NALIDIXIC-ACID IN CELLULAR DNA-REPLICATION." JOURNAL OF SUPRAMOLECULAR STRUCTURE (1978): 54-54. Link to Item
- Sugino, A, Peebles, CL, Kreuzer, KN, and Cozzarelli, NR. "Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme." Proc Natl Acad Sci U S A 74, no. 11 (November 1977): 4767-4771. Link to Item
- Kreuzer, K, Pratt, C, and Torriani, A. "Genetic analysis of regulatory mutants of alkaline phosphatase of E. coli." Genetics 81, no. 3 (1975): 459-468.
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Book Sections
- Kreuzer, KN. "Recombination-Dependent DNA Replication." In Encyclopedia of Biological Chemistry: Second Edition, 23-28. February 15, 2013. Full Text
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