Scholars@Duke

• Background
• 

  Education, Training, & Certifications

  • M.D., Northwestern University
• 

  Duke Appointment History

  • Professor of Biochemistry, Biochemistry, Basic Science Departments 1983 - 2010
  • Dean, Graduate School and Vice Provost, Graduate School, Duke University 1991 - 2006
• Research
• 

  Selected Grants

  • Biological Oxidations In Mitochondria And Microsomes awarded by National Institutes of Health 1988 - 1990
  • Mechanism Of Multielectron Reductases awarded by National Institutes of Health 1987 - 1989
• Publications & Artistic Works
• 

  Selected Publications

  • Conference Papers

    • Crane, BR, Siegel, LM, and Getzoff, ED. "Crystallographic studies of the multi-electron reductions catalyzed by the siroheme and iron-sulfur cluster-containing enzyme sulfite reductase." August 8, 1996.  Full Text

  • Journal Articles

    • Crane, BR, Siegel, LM, and Getzoff, ED. "Probing the catalytic mechanism of sulfite reductase by x-ray crystallography: Structures of the Escherichia coli hemoprotein in complex with substrates, inhibitors, intermediates, and products." BIOCHEMISTRY 36, no. 40 (October 7, 1997): 12120-12137.  Full Text  Link to Item
    • Crane, BR, Siegel, LM, and Getzoff, ED. "Structures of the siroheme- and Fe4S4-containing active center of sulfite reductase in different states of oxidation: Heme activation via reduction-gated exogenous ligand exchange." BIOCHEMISTRY 36, no. 40 (October 7, 1997): 12101-12119.  Full Text  Link to Item
    • CRANE, BR, SIEGEL, LM, and GETZOFF, ED. "SULFITE REDUCTASE STRUCTURE AT 1.6 ANGSTROM - EVOLUTION AND CATALYSIS FOR REDUCTION OF INORGANIC ANIONS." SCIENCE 270, no. 5233 (October 6, 1995): 59-67.  Full Text  Link to Item
    • Kaufman, J, Siegel, LM, and Spicer, LD. "Proton NMR of Escherichia coli sulfite reductase: studies of the heme protein subunit with added ligands." Biochemistry 32, no. 34 (August 31, 1993): 8782-8791.  Link to Item
    • Kaufman, J, Spicer, LD, and Siegel, LM. "Proton NMR of Escherichia coli sulfite reductase: the unligated hemeprotein subunit." Biochemistry 32, no. 11 (March 23, 1993): 2853-2867.  Link to Item
    • Wu, JY, Siegel, LM, and Kredich, NM. "High-level expression of Escherichia coli NADPH-sulfite reductase: requirement for a cloned cysG plasmid to overcome limiting siroheme cofactor." J Bacteriol 173, no. 1 (January 1991): 325-333.  Link to Item
    • Young, LJ, and Siegel, LM. "Alkaline low spin form of sulfite reductase hemeprotein subunit." Biochem Biophys Res Commun 169, no. 1 (May 31, 1990): 39-45.  Link to Item
    • Ostrowski, J, Barber, MJ, Rueger, DC, Miller, BE, Siegel, LM, and Kredich, NM. "Characterization of the flavoprotein moieties of NADPH-sulfite reductase from Salmonella typhimurium and Escherichia coli. Physicochemical and catalytic properties, amino acid sequence deduced from DNA sequence of cysJ, and comparison with NADPH-cytochrome P-450 reductase." J Biol Chem 264, no. 27 (September 25, 1989): 15796-15808.  Link to Item
    • Ostrowski, J, Wu, JY, Rueger, DC, Miller, BE, Siegel, LM, and Kredich, NM. "Characterization of the cysJIH regions of Salmonella typhimurium and Escherichia coli B. DNA sequences of cysI and cysH and a model for the siroheme-Fe4S4 active center of sulfite reductase hemoprotein based on amino acid homology with spinach nitrite reductase." J Biol Chem 264, no. 26 (September 15, 1989): 15726-15737.  Link to Item
    • Madden, JF, Han, SH, Siegel, LM, and Spiro, TG. "Resonance Raman studies of Escherichia coli sulfite reductase hemoprotein. 2. Fe4S4 cluster vibrational modes." Biochemistry 28, no. 13 (June 27, 1989): 5471-5477.  Link to Item
    • Han, S, Madden, JF, Siegel, LM, and Spiro, TG. "Resonance Raman studies of Escherichia coli sulfite reductase hemoprotein. 3. Bound ligand vibrational modes." Biochemistry 28, no. 13 (1989): 5477-5485.
    • Han, S, Madden, JF, Thompson, RG, Strauss, SH, Siegel, LM, and Spiro, TG. "Resonance Raman studies of Escherichia coli sulfite reductase hemoprotein. 1. Siroheme vibrational modes." Biochemistry 28, no. 13 (1989): 5461-5471.
    • HAN, S, MADDEN, JF, SIEGEL, LM, and SPIRO, TG. "NEW INSIGHTS INTO THE SIROHEME-FE4S4 INTERACTION IN SULFITE REDUCTASE FROM RESONANCE RAMAN-SPECTROSCOPY." ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 196 (September 25, 1988): 317-INOR.  Link to Item
    • Young, LJ, and Siegel, LM. "Superoxidized states of Escherichia coli sulfite reductase heme protein subunit." Biochemistry 27, no. 16 (August 9, 1988): 5984-5990.  Link to Item
    • Young, LJ, and Siegel, LM. "Activated conformers of Escherichia coli sulfite reductase heme protein subunit." Biochemistry 27, no. 14 (July 12, 1988): 4991-4999.  Link to Item
    • Young, LJ, and Siegel, LM. "On the reaction of ferric heme proteins with nitrite and sulfite." Biochemistry 27, no. 8 (April 19, 1988): 2790-2800.  Link to Item
    • DAY, EP, PETERSON, J, BONVOISIN, JJ, YOUNG, LJ, WILKERSON, JO, and SIEGEL, LM. "MAGNETIZATION OF THE SULFITE AND NITRITE COMPLEXES OF OXIDIZED SULFITE AND NITRITE REDUCTASES - ELECTRON-PARAMAGNETIC-RES SILENT SPIN S=1/2 STATES." BIOCHEMISTRY 27, no. 6 (March 22, 1988): 2126-2132.  Full Text  Link to Item
    • DAY, EP, BONVOISIN, J, PETERSON, J, WILKERSON, J, YOUNG, LJ, and SIEGEL, LM. "THE SULFITE COMPLEX OF SULFITE REDUCTASE - ELECTRON-PARAMAGNETIC-RES SILENT SPIN S= 1/2 STATE." ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 194 (August 30, 1987): 134-INOR.  Link to Item
    • OSTROWSKI, J, BACK, EW, MADDEN, J, SIEGEL, LM, and KREDICH, NM. "COMPARISON OF DEDUCED AMINO-ACID-SEQUENCES OF ALPHA-SUBUNIT AND BETA-SUBUNIT OF SALMONELLA-TYPHIMURIUM NADPH-SULFITE REDUCTASE (SR) WITH THOSE OF SPINACH NITRITE REDUCTASE (NR) AND CYTOCHROME-P-450 OXIDOREDUCTASE (P450R)." FEDERATION PROCEEDINGS 46, no. 6 (May 1, 1987): 2231-2231.  Link to Item
    • CLINE, JF, JANICK, PA, SIEGEL, LM, and HOFFMAN, BM. "FE-57 AND H-1 ELECTRON NUCLEAR DOUBLE-RESONANCE OF 3 DOUBLY REDUCED STATES OF ESCHERICHIA-COLI SULFITE REDUCTASE." BIOCHEMISTRY 25, no. 16 (August 12, 1986): 4647-4654.  Full Text  Link to Item
    • McRee, DE, Richardson, DC, Richardson, JS, and Siegel, LM. "The heme and Fe4S4 cluster in the crystallographic structure of Escherichia coli sulfite reductase." J Biol Chem 261, no. 22 (August 5, 1986): 10277-10281.  Link to Item
    • Cline, JF, Janick, PA, Siegel, LM, and Hoffman, BM. "57Fe and 1H Electron-Nuclear Double Resonance of Three Doubly Reduced States of Escherichia coli Sulfite Reductase." Biochemistry 25, no. 16 (January 1, 1986): 4647-4654.  Full Text
    • McRee, DE, Richardson, DC, Richardson, JS, and Siegel, LM. "The heme and Fe4S4cluster in the crystallographic structure of Escherichia coli sulfite reductase." Journal of Biological Chemistry 261, no. 22 (January 1, 1986): 10277-10281.
    • CLINE, JF, JANICK, PA, SIEGEL, LM, and HOFFMAN, BM. "ELECTRON NUCLEAR DOUBLE-RESONANCE STUDIES OF OXIDIZED ESCHERICHIA-COLI SULFITE REDUCTASE - H-1, N-14, AND FE-57 MEASUREMENTS." BIOCHEMISTRY 24, no. 27 (December 31, 1985): 7942-7947.  Full Text  Link to Item
    • CHRISTNER, JA, MUNCK, E, KENT, TA, JANICK, PA, SALERNO, JC, and SIEGEL, LM. "EXCHANGE COUPLING BETWEEN SIROHEME AND [4FE-4S] CLUSTER IN ESCHERICHIA-COLI SULFITE REDUCTASE - MOSSBAUER STUDIES AND COUPLING MODELS FOR A 2-ELECTRON REDUCED ENZYME STATE AND COMPLEXES WITH SULFIDE." JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 106, no. 22 (1984): 6786-6794.  Full Text  Link to Item
    • COUGHLAN, MP, MEHRA, RK, BARBER, MJ, and SIEGEL, LM. "OPTICAL AND ELECTRON-PARAMAGNETIC RESONANCE SPECTROSCOPIC STUDIES ON PURINE HYDROXYLASE II FROM ASPERGILLUS-NIDULANS." ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 229, no. 2 (1984): 596-603.  Full Text  Link to Item
    • BARBER, MJ, ROSEN, GM, SIEGEL, LM, and RAUCKMAN, EJ. "EVIDENCE FOR FORMATION OF SUPEROXIDE AND FORMATE RADICALS IN METHANOBACTERIUM-FORMICICUM." JOURNAL OF BACTERIOLOGY 153, no. 3 (1983): 1282-1286.  Link to Item
    • BARBER, MJ, SIEGEL, LM, SCHAUER, NL, MAY, HD, and FERRY, JG. "FORMATE DEHYDROGENASE FROM METHANOBACTERIUM-FORMICICUM - ELECTRON-PARAMAGNETIC RESONANCE SPECTROSCOPY OF THE MOLYBDENUM AND IRON-SULFUR CENTERS." JOURNAL OF BIOLOGICAL CHEMISTRY 258, no. 18 (1983): 839-845.  Link to Item
    • BARBER, MJ, and SIEGEL, LM. "ELECTRON-PARAMAGNETIC RESONANCE AND POTENTIOMETRIC STUDIES OF ARSENITE INTERACTION WITH THE MOLYBDENUM CENTERS OF XANTHINE-OXIDASE, XANTHINE DEHYDROGENASE, AND ALDEHYDE OXIDASE - A SPECIFIC STABILIZATION OF THE MOLYBDENUM(V) OXIDATION-STATE." BIOCHEMISTRY 22, no. 3 (1983): 618-624.  Full Text  Link to Item
    • CHRISTNER, JA, JANICK, PA, SIEGEL, LM, and MUNCK, E. "MOSSBAUER STUDIES OF ESCHERICHIA-COLI SULFITE REDUCTASE COMPLEXES WITH CARBON-MONOXIDE AND CYANIDE - EXCHANGE COUPLING AND INTRINSIC-PROPERTIES OF THE [4FE-4S] CLUSTER." JOURNAL OF BIOLOGICAL CHEMISTRY 258, no. 18 (1983): 1157-1164.  Link to Item
    • CHRISTNER, JA, MUNCK, E, JANICK, PA, and SIEGEL, LM. "MOSSBAUER EVIDENCE FOR EXCHANGE-COUPLED SIROHEME AND [4FE-4S] PROSTHETIC GROUPS IN ESCHERICHIA-COLI SULFITE REDUCTASE - STUDIES OF THE REDUCED STATES AND OF A NITRITE TURNOVER COMPLEX." JOURNAL OF BIOLOGICAL CHEMISTRY 258, no. 18 (1983): 1147-1156.  Link to Item
    • JANICK, PA, RUEGER, DC, KRUEGER, RJ, BARBER, MJ, and SIEGEL, LM. "CHARACTERIZATION OF COMPLEXES BETWEEN ESCHERICHIA-COLI SULFITE REDUCTASE HEMOPROTEIN SUBUNIT AND ITS SUBSTRATES SULFITE AND NITRITE." BIOCHEMISTRY 22, no. 2 (1983): 396-408.  Full Text  Link to Item
    • JANICK, PA, and SIEGEL, LM. "ELECTRON-PARAMAGNETIC RESONANCE AND OPTICAL EVIDENCE FOR INTERACTION BETWEEN SIROHEME AND FE4S4 PROSTHETIC GROUPS IN COMPLEXES OF ESCHERICHIA-COLI SULFITE REDUCTASE HEMOPROTEIN WITH ADDED LIGANDS." BIOCHEMISTRY 22, no. 2 (1983): 504-515.  Full Text  Link to Item
    • MUNCK, E, CHRISTNER, JA, JANICK, PA, and SIEGEL, LM. "A NOVEL SYSTEM INVOLVING EXCHANGE-COUPLING BETWEEN A HEME AND AN IRON SULFUR CLUSTER." INORGANICA CHIMICA ACTA-BIOINORGANIC CHEMISTRY 79, no. 1-6 (1983): 20-20.  Full Text  Link to Item
    • WILKERSON, JO, JANICK, PA, and SIEGEL, LM. "SIROHEME-FE4S4 INTERACTION IN SPINACH NITRITE REDUCTASE (NIR)." FEDERATION PROCEEDINGS 42, no. 7 (1983): 2060-2060.  Link to Item
    • WILKERSON, JO, JANICK, PA, and SIEGEL, LM. "ELECTRON-PARAMAGNETIC RESONANCE AND OPTICAL SPECTROSCOPIC EVIDENCE FOR INTERACTION BETWEEN SIROHEME AND TETRANUCLEAR IRON SULFUR CENTER PROSTHETIC GROUPS IN SPINACH FERREDOXIN NITRITE REDUCTASE." BIOCHEMISTRY 22, no. 21 (1983): 5048-5054.  Full Text  Link to Item
    • Barber, MJ, Coughlan, MP, Rajagopalan, KV, and Siegel, LM. "Properties of the prosthetic groups of rabbit liver aldehyde oxidase: a comparison of molybdenum hydroxylase enzymes." Biochemistry 21, no. 15 (July 20, 1982): 3561-3568.  Link to Item
    • BARBER, MJ, SALERNO, JC, and SIEGEL, LM. "MAGNETIC-INTERACTIONS IN MILK XANTHINE-OXIDASE." BIOCHEMISTRY 21, no. 7 (1982): 1648-1656.  Full Text  Link to Item
    • BARBER, MJ, SIEGEL, LM, SCHAUER, NL, MAY, HD, and FERRY, JG. "METHANOGENESIS - A NOVEL MO CENTER IN METHANOBACTERIUM-FORMICICUM." FEDERATION PROCEEDINGS 41, no. 4 (1982): 891-891.  Link to Item
    • BARBER, MJ, and SIEGEL, LM. "OXIDATION REDUCTION POTENTIALS OF MOLYBDENUM, FLAVIN, AND IRON SULFUR CENTERS IN MILK XANTHINE-OXIDASE - VARIATION WITH PH." BIOCHEMISTRY 21, no. 7 (1982): 1638-1647.  Full Text  Link to Item
    • JANICK, PA, and SIEGEL, LM. "ELECTRON-PARAMAGNETIC RESONANCE AND OPTICAL SPECTROSCOPIC EVIDENCE FOR INTERACTION BETWEEN SIROHEME AND FE4S4 PROSTHETIC GROUPS IN ESCHERICHIA-COLI SULFITE REDUCTASE HEMOPROTEIN SUBUNIT." BIOCHEMISTRY 21, no. 15 (1982): 3538-3547.  Full Text  Link to Item
    • KRUEGER, RJ, and SIEGEL, LM. "SPINACH SIROHEME ENZYMES - ISOLATION AND CHARACTERIZATION OF FERREDOXIN SULFITE REDUCTASE AND COMPARISON OF PROPERTIES WITH FERREDOXIN NITRITE REDUCTASE." BIOCHEMISTRY 21, no. 12 (1982): 2892-2904.  Full Text  Link to Item
    • KRUEGER, RJ, and SIEGEL, LM. "EVIDENCE FOR SIROHEME-FE4S4 INTERACTION IN SPINACH FERREDOXIN SULFITE REDUCTASE." BIOCHEMISTRY 21, no. 12 (1982): 2905-2909.  Full Text  Link to Item
    • SIEGEL, LM, RUEGER, DC, BARBER, MJ, KRUEGER, RJ, ORMEJOHNSON, NR, and ORMEJOHNSON, WH. "ESCHERICHIA-COLI SULFITE REDUCTASE HEMOPROTEIN SUBUNIT - PROSTHETIC GROUPS, CATALYTIC PARAMETERS, AND LIGAND COMPLEXES." JOURNAL OF BIOLOGICAL CHEMISTRY 257, no. 11 (1982): 6343-6350.  Link to Item
    • SPENCE, JT, BARBER, MJ, and SIEGEL, LM. "DETERMINATION OF THE STOICHIOMETRY OF ELECTRON UPTAKE AND THE MIDPOINT REDUCTION POTENTIALS OF MILK XANTHINE-OXIDASE AT 25-DEGREES-C BY MICROCOULOMETRY." BIOCHEMISTRY 21, no. 7 (1982): 1656-1661.  Full Text  Link to Item
    • BARBER, MJ, SALERNO, JC, and SIEGEL, LM. "MAGNETIC-INTERACTIONS IN XANTHINE-OXIDASE (XO)." FEDERATION PROCEEDINGS 40, no. 6 (1981): 1665-1665.  Link to Item
    • CHRISTNER, JA, MUNCK, E, JANICK, PA, and SIEGEL, LM. "MOSSBAUER SPECTROSCOPIC STUDIES OF ESCHERICHIA-COLI SULFITE REDUCTASE - EVIDENCE FOR COUPLING BETWEEN THE SIROHEME AND FE4S4 CLUSTER PROSTHETIC GROUPS." JOURNAL OF BIOLOGICAL CHEMISTRY 256, no. 5 (1981): 2098-2101.  Link to Item
    • BARBER, MJ, and SIEGEL, LM. "EFFECT OF PH ON THE REDOX POTENTIALS OF MILK XANTHINE-OXIDASE (XO)." FEDERATION PROCEEDINGS 39, no. 6 (1980): 1677-1677.  Link to Item
    • LANCASTER, JR, VEGA, JM, KAMIN, H, ORMEJOHNSON, NR, ORMEJOHNSON, WH, KRUEGER, RJ, and SIEGEL, LM. "IDENTIFICATION OF THE IRON-SULFUR CENTER OF SPINACH FERREDOXIN NITRITE REDUCTASE AS A TETRANUCLEAR CENTER, AND PRELIMINARY EPR STUDIES OF MECHANISM." JOURNAL OF BIOLOGICAL CHEMISTRY 254, no. 4 (1979): 1268-1272.  Link to Item
    • KRUEGER, R, and SIEGEL, LM. "SPINACH-FERREDOXIN-SULFITE REDUCTASE (SIR)." PLANT PHYSIOLOGY 61, no. 4 (1978): 67-67.  Link to Item
    • SCOTT, AI, IRWIN, AJ, SIEGEL, LM, and SHOOLERY, JN. "SIROHYDROCHLORIN - PROSTHETIC GROUP OF SULFITE AND NITRITE REDUCTASES AND ITS ROLE IN BIOSYNTHESIS OF VITAMIN-B-12." JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 100, no. 25 (1978): 7987-7994.  Full Text  Link to Item
    • SCOTT, AI, IRWIN, AJ, SIEGEL, LM, and SHOOLERY, JN. "SIROHYDROCHLORIN - PROSTHETIC GROUP OF A SULFITE REDUCTASE ENZYME AND ITS ROLE IN BIOSYNTHESIS OF VITAMIN-B12." JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 100, no. 1 (1978): 316-318.  Full Text  Link to Item
    • SIEGEL, LM, DAVIS, PS, and MURPHY, MJ. "INCORPORATION OF METHIONINE-DERIVED METHYL-GROUPS INTO SIROHAEM BY ESCHERICHIA-COLI." BIOCHEMICAL JOURNAL 167, no. 3 (1977): 669-674.  Full Text  Link to Item
    • VEGA, JM, GARRETT, RH, and SIEGEL, LM. "SIROHEME - PROSTHETIC GROUP OF NEUROSPORA-CRASSA ASSIMILATORY NITRITE REDUCTASE." JOURNAL OF BIOLOGICAL CHEMISTRY 250, no. 20 (1975): 7980-7989.  Link to Item
    • Faeder, EJ, Davis, PS, and Siegel, LM. "Reduced nicotinamide adenine dinucleotide phosphate sulfite reductase of enterobacteria. V. Studies with the Escherichia coli hemoflavoprotein depleted of flavin mononucleotide: distinct roles for the flavin adenine dinucleotide and flavin mononucleotide prosthetic groups in catalysis." Journal of Biological Chemistry 249, no. 5 (January 1, 1974): 1599-1609.
    • Murphy, MJ, Siegel, LM, Tove, SR, and Kamin, H. "Siroheme: A new prosthetic group participating in six electron reduction reactions catalyzed by both sulfite and nitrite reductases." Proceedings of the National Academy of Sciences of the United States of America 71, no. 3 (January 1, 1974): 612-616.  Full Text
    • Murphy, MJ, Siegel, LM, and Kamin, H. "Reduced nicotinamide adenine dinucleotide phosphate sulfite reductase of enterobacteria. VI. The reaction of carbon monoxide with the Escherichia coli holoenzyme, the hemoprotein, and free siroheme." Journal of Biological Chemistry 249, no. 5 (January 1, 1974): 1610-1614.
    • Siegel, LM, Davis, PS, and Kamin, H. "Reduced nicotinamide adenine dinucleotide phosphate sulfite reductase of enterobacteria. III. The Escherichia coli hemoflavoprotein: catalytic parameters and the sequence of electron flow." Journal of Biological Chemistry 249, no. 5 (January 1, 1974): 1572-1586.
    • Siegel, LM, and Davis, PS. "Reduced nicotinamide adenine dinucleotide phosphate sulfite reductase of enterobacteria. IV. The Escherichia coli hemoflavoprotein: subunit structure and dissociation into hemoprotein and flavoprotein components." Journal of Biological Chemistry 249, no. 5 (January 1, 1974): 1587-1598.
    • Murphy, MJ, and Siegel, LM. "Siroheme and sirohydrochlorin. The basis for a new type of porphyrin related prosthetic group common to both assimilatory and dissimilatory sulfite reductases." Journal of Biological Chemistry 248, no. 19 (December 1, 1973): 6911-6919.
    • Murphy, MJ, Siegel, LM, Kamin, H, DerVartanian, DV, Lee, JP, LeGall, J, and Peck, HD. "An iron tetrahydroporphyrin prosthetic group common to both assimilatory and dissimilatory sulfite reductases." Biochemical and Biophysical Research Communications 54, no. 1 (September 5, 1973): 82-88.  Full Text
    • Faeder, EJ, and Siegel, LM. "A rapid micromethod for determination of FMN and FAD in mixtures." Analytical Biochemistry 53, no. 1 (January 1, 1973): 332-336.  Full Text
    • Siegel, LM, Faeder, EJ, and Kamin, H. "Flavin interaction in NADPH-sulfite reductase." Zeitschrift fur Naturforschung. Teil B. Anorganische Chemie, organische Chemie, Biochemie, Biophysik, Biologie 27, no. 9 (September 1972): 1087-1089.

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