Scholars@Duke
Douglas Joe Raburn

Douglas Joe Raburn

Assistant Professor of Obstetrics and Gynecology
Obstetrics and Gynecology, Reproductive Endocrinology & Infertility
rabur001@mc.duke.edu
Box 3144 Med Ctr, Durham, NC 27710
5601 Arringdon Park Drive, Morrisville, NC 27560
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Selected Publications

Differential Gene Regulation of the Human Blastocyst Trophectoderm and Inner Cell Mass by Progesterone.

Journal Article Reprod Sci · May 2024 Knowledge of action of progesterone (P4) on the human preimplantation embryo is lacking. The objective of this study was to determine expression of a mitochondrial P4 receptor (PR-M) in the trophectoderm (TE) and the inner cell mass (ICM) of the human blas ... Full text Link to item Cite

Association between donor gamete use and supernumerary embryo disposition decisions.

Journal Article J Assist Reprod Genet · February 2023 PURPOSE: The aim of this study is to determine if donor gamete use is associated with patients' decisions regarding disposition of supernumerary embryos. METHODS: Patients who intended to undergo an IVF cycle at a single academic center signed an embryo di ... Full text Open Access Link to item Cite

Male obesity impacts DNA methylation reprogramming in sperm.

Journal Article Clin Epigenetics · January 25, 2021 BACKGROUND: Male obesity has profound effects on morbidity and mortality, but relatively little is known about the impact of obesity on gametes and the potential for adverse effects of male obesity to be passed to the next generation. DNA methylation contr ... Full text Open Access Link to item Cite

Refraining from use diminishes cannabis-associated epigenetic changes in human sperm.

Journal Article Environ Epigenet · 2021 Cannabis use alters sperm DNA methylation, but the potential reversibility of these changes is unknown. Semen samples from cannabis users and non-user controls were collected at baseline and again following a 77-day period of cannabis abstinence (one sperm ... Full text Open Access Link to item Cite

Data from: Cannabinoid exposure and altered DNA methylation in rat and human sperm

Dataset · August 25, 2020 This dataset contains the raw sequencing data files for reduced representation bisulfite sequencing. Abstract: Little is known about the reproductive effects of paternal cannabis exposure. We evaluated associations between cannabis or tetrahydrocannabinol ... Full text Cite

Progesterone Increases Mitochondria Membrane Potential in Non-human Primate Oocytes and Embryos.

Journal Article Reprod Sci · May 2020 Mitochondrial activity is critical and correlates with embryo development. The identification of a novel human mitochondrial progesterone receptor (PR-M) that increases cellular respiration brings into question a role for progesterone in oocyte and preimpl ... Full text Link to item Cite

Patient and in vitro fertilization (IVF) cycle characteristics associated with variable blastulation rates: a retrospective study from the Duke Fertility Center (2013–2017)

Journal Article Middle East Fertility Society Journal · December 1, 2019 Background: To evaluate the association of patient and IVF cycle characteristics with blastulation rate and formation of high-quality blastocysts Results: We analyzed autologous blastocyst cycles from 2013 to 2017. Cycles were subdivided into low (< 33%), ... Full text Cite

Does a freeze-all policy lead to better IVF outcomes in first autologous cycles?

Journal Article Middle East Fertility Society Journal · December 1, 2018 Full text Cite

Freezing of all embryos in in vitro fertilization is beneficial in high responders, but not intermediate and low responders: an analysis of 82,935 cycles from the Society for Assisted Reproductive Technology registry.

Journal Article Fertil Steril · October 2018 OBJECTIVE: To assess in vitro fertilization (IVF) and pregnancy outcomes in patients having their first frozen embryo transfer (FET) after a freeze-all cycle versus similar patients having their first fresh embryo transfer (ET). DESIGN: Retrospective cohor ... Full text Link to item Cite

Cannabinoid exposure and altered DNA methylation in rat and human sperm.

Journal Article Epigenetics · 2018 Little is known about the reproductive effects of paternal cannabis exposure. We evaluated associations between cannabis or tetrahydrocannabinol (THC) exposure and altered DNA methylation in sperm from humans and rats, respectively. DNA methylation, measur ... Full text Open Access Link to item Cite

The effect of progesterone on early embryo metabolism

Conference Fertility and Sterility · September 2016 Full text Cite

Leukocytospermia: Overview of diagnosis, implications, and management of a controversial finding

Journal Article Middle East Fertility Society Journal · September 1, 2013 Background The progress in assisted reproductive techniques over the last 30 years has raised interest in studying what in 1992 the World Health Organization (WHO) defined as abnormal: the presence of >1 × 10 6 leukocytes/mL in a semen sample. Leukocytospe ... Full text Cite

Relationship between pre-embryo pronuclear morphology (zygote score) and standard day 2 or 3 embryo morphology with regard to assisted reproductive technique outcomes.

Journal Article Fertil Steril · October 2005 OBJECTIVE: To test the hypothesis that pregnancy rates are low if grade Z1 pre-embryos are not available for transfer and to determine if pronuclear morphology is a better predictor of pregnancy than traditional embryo morphology. DESIGN: Prospective clini ... Full text Link to item Cite

Redefining the relationship between sperm deoxyribonucleic acid fragmentation as measured by the sperm chromatin structure assay and outcomes of assisted reproductive techniques.

Journal Article Fertil Steril · August 2005 OBJECTIVE: To test the hypothesis that couples with sperm chromatin structure assay (SCSA) DNA fragmentation index (DFI) values >27% would not achieve pregnancy with assisted reproductive techniques (ART) and to investigate how DFI and high DNA stainabilit ... Full text Link to item Cite

Antiretroviral therapy effects on genetic and morphologic end points in lymphocytes and sperm of men with human immunodeficiency virus infection.

Journal Article J Infect Dis · July 15, 2001 Many human immunodeficiency virus (HIV)-infected persons receive prolonged treatment with DNA-reactive antiretroviral drugs. A prospective study was conducted of 26 HIV-infected men who provided samples before treatment and at multiple times after beginnin ... Full text Link to item Cite

Differential Gene Regulation of the Human Blastocyst Trophectoderm and Inner Cell Mass by Progesterone.

Journal Article Reprod Sci · May 2024 Knowledge of action of progesterone (P4) on the human preimplantation embryo is lacking. The objective of this study was to determine expression of a mitochondrial P4 receptor (PR-M) in the trophectoderm (TE) and the inner cell mass (ICM) of the human blas ... Full text Link to item Cite

Association between donor gamete use and supernumerary embryo disposition decisions.

Journal Article J Assist Reprod Genet · February 2023 PURPOSE: The aim of this study is to determine if donor gamete use is associated with patients' decisions regarding disposition of supernumerary embryos. METHODS: Patients who intended to undergo an IVF cycle at a single academic center signed an embryo di ... Full text Open Access Link to item Cite

Male obesity impacts DNA methylation reprogramming in sperm.

Journal Article Clin Epigenetics · January 25, 2021 BACKGROUND: Male obesity has profound effects on morbidity and mortality, but relatively little is known about the impact of obesity on gametes and the potential for adverse effects of male obesity to be passed to the next generation. DNA methylation contr ... Full text Open Access Link to item Cite

Refraining from use diminishes cannabis-associated epigenetic changes in human sperm.

Journal Article Environ Epigenet · 2021 Cannabis use alters sperm DNA methylation, but the potential reversibility of these changes is unknown. Semen samples from cannabis users and non-user controls were collected at baseline and again following a 77-day period of cannabis abstinence (one sperm ... Full text Open Access Link to item Cite

Data from: Cannabinoid exposure and altered DNA methylation in rat and human sperm

Dataset · August 25, 2020 This dataset contains the raw sequencing data files for reduced representation bisulfite sequencing. Abstract: Little is known about the reproductive effects of paternal cannabis exposure. We evaluated associations between cannabis or tetrahydrocannabinol ... Full text Cite

Progesterone Increases Mitochondria Membrane Potential in Non-human Primate Oocytes and Embryos.

Journal Article Reprod Sci · May 2020 Mitochondrial activity is critical and correlates with embryo development. The identification of a novel human mitochondrial progesterone receptor (PR-M) that increases cellular respiration brings into question a role for progesterone in oocyte and preimpl ... Full text Link to item Cite

Patient and in vitro fertilization (IVF) cycle characteristics associated with variable blastulation rates: a retrospective study from the Duke Fertility Center (2013–2017)

Journal Article Middle East Fertility Society Journal · December 1, 2019 Background: To evaluate the association of patient and IVF cycle characteristics with blastulation rate and formation of high-quality blastocysts Results: We analyzed autologous blastocyst cycles from 2013 to 2017. Cycles were subdivided into low (< 33%), ... Full text Cite

Does a freeze-all policy lead to better IVF outcomes in first autologous cycles?

Journal Article Middle East Fertility Society Journal · December 1, 2018 Full text Cite

Freezing of all embryos in in vitro fertilization is beneficial in high responders, but not intermediate and low responders: an analysis of 82,935 cycles from the Society for Assisted Reproductive Technology registry.

Journal Article Fertil Steril · October 2018 OBJECTIVE: To assess in vitro fertilization (IVF) and pregnancy outcomes in patients having their first frozen embryo transfer (FET) after a freeze-all cycle versus similar patients having their first fresh embryo transfer (ET). DESIGN: Retrospective cohor ... Full text Link to item Cite

Cannabinoid exposure and altered DNA methylation in rat and human sperm.

Journal Article Epigenetics · 2018 Little is known about the reproductive effects of paternal cannabis exposure. We evaluated associations between cannabis or tetrahydrocannabinol (THC) exposure and altered DNA methylation in sperm from humans and rats, respectively. DNA methylation, measur ... Full text Open Access Link to item Cite

The effect of progesterone on early embryo metabolism

Conference Fertility and Sterility · September 2016 Full text Cite

Leukocytospermia: Overview of diagnosis, implications, and management of a controversial finding

Journal Article Middle East Fertility Society Journal · September 1, 2013 Background The progress in assisted reproductive techniques over the last 30 years has raised interest in studying what in 1992 the World Health Organization (WHO) defined as abnormal: the presence of >1 × 10 6 leukocytes/mL in a semen sample. Leukocytospe ... Full text Cite

Relationship between pre-embryo pronuclear morphology (zygote score) and standard day 2 or 3 embryo morphology with regard to assisted reproductive technique outcomes.

Journal Article Fertil Steril · October 2005 OBJECTIVE: To test the hypothesis that pregnancy rates are low if grade Z1 pre-embryos are not available for transfer and to determine if pronuclear morphology is a better predictor of pregnancy than traditional embryo morphology. DESIGN: Prospective clini ... Full text Link to item Cite

Redefining the relationship between sperm deoxyribonucleic acid fragmentation as measured by the sperm chromatin structure assay and outcomes of assisted reproductive techniques.

Journal Article Fertil Steril · August 2005 OBJECTIVE: To test the hypothesis that couples with sperm chromatin structure assay (SCSA) DNA fragmentation index (DFI) values >27% would not achieve pregnancy with assisted reproductive techniques (ART) and to investigate how DFI and high DNA stainabilit ... Full text Link to item Cite

Antiretroviral therapy effects on genetic and morphologic end points in lymphocytes and sperm of men with human immunodeficiency virus infection.

Journal Article J Infect Dis · July 15, 2001 Many human immunodeficiency virus (HIV)-infected persons receive prolonged treatment with DNA-reactive antiretroviral drugs. A prospective study was conducted of 26 HIV-infected men who provided samples before treatment and at multiple times after beginnin ... Full text Link to item Cite

Stage-specific expression of B cell translocation gene 1 in rat testis.

Journal Article Endocrinology · December 1995 B Cell translocation gene 1 (BTG1) is a member of a new family of putative antiproliferative factors. They are characterized by their rapid, but transient, expression in response to factors that induce growth arrest and subsequent differentiation. In immat ... Full text Link to item Cite

REGULATION OF THE MACROPHAGE POPULATION IN POSTNATAL RAT TESTIS (VOL 24, PG 139, 1993)

Journal Article JOURNAL OF REPRODUCTIVE IMMUNOLOGY · November 1, 1993 Link to item Cite

Regulation of the macrophage population in postnatal rat testis.

Journal Article J Reprod Immunol · July 1993 Testicular macrophages increase in concentration during postnatal development in rats. This process may be under hormonal control since administration of hCG stimulates a similar increase to occur precociously. The purpose of the present studies was to det ... Full text Link to item Cite

EFFECTS OF TESTOSTERONE AND CASODEX ON TESTICULAR MACROPHAGES

Conference MOLECULAR BIOLOGY OF THE CELL · September 1, 1992 Link to item Cite

Human chorionic gonadotropin increases the concentration of macrophages in neonatal rat testis.

Journal Article Biol Reprod · July 1991 The purpose of these studies was to determine whether treatment of newborn rats with exogenous FSH or hCG would alter the concentration or size of testicular macrophages. Animals were injected once daily with various doses of FSH, hCG, or vehicle for 8-10 ... Full text Link to item Cite