Scholars@Duke

• Background
• 

  Education, Training, & Certifications

  • Ph.D., University of Wisconsin at Madison 1984
• 

  Duke Appointment History

  • Consulting Professor in the Department of Biochemistry, Biochemistry, Basic Science Departments 2011
• Recognition
• 

  In the News

  • SEP 21, 2018

    School of Medicine News Blog

    Basic Science Day Showcases Wide Spectrum of Discovery Research at Duke

  • SEP 6, 2017

    Molecular Map Shows How to Disable Dangerous Bioweapon

  • JUN 14, 2016

    Protein Structures Pave The Way For Broad-Spectrum Antifungals

  • JUL 29, 2015

    Structures Reveal Basis of Recurring Urinary Tract Infections
• 

  Awards & Honors

  • AAAS Fellow. American Association for the Advancement of Science. 2011
  • Fellow of the American Academy of Microbiology. The American Academy of Microbiology (AAM). 2007
• Research
• 

  Selected Grants

  • Investigating a Novel Co-regulation of Multi-Drug Efflux Pumps and Polysaccharide Capsule in E. coli awarded by National Institutes of Health 2017 - 2021
  • Organization and Function of Cellular Structure awarded by National Institutes of Health 1975 - 2020
  • Molecular Mycology and Pathogenesis Training Program awarded by National Institutes of Health 2002 - 2019
  • Biology of the HMW1 and HMW2 Adhesins of H. Influenzae awarded by Children's Hospital of Philadelphia 2013 - 2019
  • Defining the structural mechanisms of Hfq binding to cognate nucleic acids awarded by National Institutes of Health 2015 - 2017
  • Structural Biology and Biophysics Training Program awarded by National Institutes of Health 1994 - 2015
  • High sensitivity multi-purpose electron paramagnetic resonance spectroscopy for biotechnological and biomedical research awarded by North Carolina Biotechnology Center 2014 - 2015
• Publications & Artistic Works
• 

  Selected Publications

  • Academic Articles

    • Schumacher, MA, den Hengst, CD, Bush, MJ, Le, TBK, Tran, NT, Chandra, G, Zeng, W, Travis, B, Brennan, RG, and Buttner, MJ. "The MerR-like protein BldC binds DNA direct repeats as cooperative multimers to regulate Streptomyces development." Nature Communications 9, no. 1 (March 19, 2018): 1139-null.  Full Text
    • Cuthbert, BJ, Ross, W, Rohlfing, AE, Dove, SL, Gourse, RL, Brennan, RG, and Schumacher, MA. "Dissection of the molecular circuitry controlling virulence in Francisella tularensis." Genes & development 31, no. 15 (August 2017): 1549-1560.  Full Text
    • Miao, Y, Tenor, JL, Toffaletti, DL, Maskarinec, SA, Liu, J, Lee, RE, Perfect, JR, and Brennan, RG. "Structural and In Vivo Studies on Trehalose-6-Phosphate Synthase from Pathogenic Fungi Provide Insights into Its Catalytic Mechanism, Biological Necessity, and Potential for Novel Antifungal Drug Design." Mbio 8, no. 4 (July 25, 2017).  Full Text
    • Schumacher, MA, Zeng, W, Findlay, KC, Buttner, MJ, Brennan, RG, and Tschowri, N. "The Streptomyces master regulator BldD binds c-di-GMP sequentially to create a functional BldD2-(c-di-GMP)4 complex." Nucleic Acids Research 45, no. 11 (June 2017): 6923-6933.  Full Text
    • Thammahong, A, Puttikamonkul, S, Perfect, JR, Brennan, RG, and Cramer, RA. "Central Role of the Trehalose Biosynthesis Pathway in the Pathogenesis of Human Fungal Infections: Opportunities and Challenges for Therapeutic Development." Microbiology and molecular biology reviews : MMBR 81, no. 2 (June 2017). (Review)  Full Text
    • Perfect, JR, Tenor, JL, Miao, Y, and Brennan, RG. "Trehalose pathway as an antifungal target." Virulence 8, no. 2 (February 2017): 143-149. (Review)  Full Text
    • Miao, Y, Tenor, JL, Toffaletti, DL, Washington, EJ, Liu, J, Shadrick, WR, Schumacher, MA, Lee, RE, Perfect, JR, and Brennan, RG. "Structures of trehalose-6-phosphate phosphatase from pathogenic fungi reveal the mechanisms of substrate recognition and catalysis." Proceedings of the National Academy of Sciences of the United States of America 113, no. 26 (June 15, 2016): 7148-7153.  Full Text
    • Schumacher, MA, Balani, P, Min, J, Chinnam, NB, Hansen, S, Vulić, M, Lewis, K, and Brennan, RG. "HipBA-promoter structures reveal the basis of heritable multidrug tolerance." Nature 524, no. 7563 (August 2015): 59-64.  Full Text
    • Schindler, BD, Seo, SM, Birukou, I, Brennan, RG, and Kaatz, GW. "Mutations within the mepA operator affect binding of the MepR regulatory protein and its induction by MepA substrates in Staphylococcus aureus." Journal of Bacteriology 197, no. 6 (March 2015): 1104-1114.  Full Text
    • Cuthbert, BJ, Brennan, RG, and Schumacher, MA. "Structural and Biochemical Characterization of the Francisella tularensis Pathogenicity Regulator, Macrophage Locus Protein A (MglA)." PloS one 10, no. 6 (January 2015): e0128225-.  Full Text
    • Reniere, ML, Whiteley, AT, Hamilton, KL, John, SM, Lauer, P, Brennan, RG, and Portnoy, DA. "Glutathione activates virulence gene expression of an intracellular pathogen." Nature 517, no. 7533 (January 2015): 170-173.  Full Text
    • Kovach, AR, Hoff, KE, Canty, JT, Orans, J, and Brennan, RG. "Recognition of U-rich RNA by Hfq from the Gram-positive pathogen Listeria monocytogenes." RNA (New York, N.Y.) 20, no. 10 (October 2014): 1548-1559.  Full Text
    • Tschowri, N, Schumacher, MA, Schlimpert, S, Chinnam, NB, Findlay, KC, Brennan, RG, and Buttner, MJ. "Tetrameric c-di-GMP mediates effective transcription factor dimerization to control Streptomyces development." Cell 158, no. 5 (August 2014): 1136-1147.  Full Text
    • Birukou, I, Seo, SM, Schindler, BD, Kaatz, GW, and Brennan, RG. "Structural mechanism of transcription regulation of the Staphylococcus aureus multidrug efflux operon mepRA by the MarR family repressor MepR." Nucleic Acids Research 42, no. 4 (February 2014): 2774-2788.  Full Text
    • Robinson, KE, Orans, J, Kovach, AR, Link, TM, and Brennan, RG. "Mapping Hfq-RNA interaction surfaces using tryptophan fluorescence quenching." Nucleic Acids Research 42, no. 4 (February 2014): 2736-2749.  Full Text
    • Birukou, I, Tonthat, NK, Seo, SM, Schindler, BD, Kaatz, GW, and Brennan, RG. "The molecular mechanisms of allosteric mutations impairing MepR repressor function in multidrug-resistant strains of Staphylococcus aureus." Mbio 4, no. 5 (August 27, 2013): e00528-e00513.  Full Text
    • Schindler, BD, Seo, SM, Jacinto, PL, Kumaraswami, M, Birukou, I, Brennan, RG, and Kaatz, GW. "Functional consequences of substitution mutations in MepR, a repressor of the Staphylococcus aureus MepA multidrug efflux pump gene." Journal of Bacteriology 195, no. 16 (August 2013): 3651-3662.  Full Text
    • Xie, T-X, Zhou, G, Zhao, M, Sano, D, Jasser, SA, Brennan, RG, and Myers, JN. "Serine substitution of proline at codon 151 of TP53 confers gain of function activity leading to anoikis resistance and tumor progression of head and neck cancer cells." The Laryngoscope 123, no. 6 (June 2013): 1416-1423.  Full Text
    • Rajagopalan, S, Teter, SJ, Zwart, PH, Brennan, RG, Phillips, KJ, and Kiley, PJ. "Studies of IscR reveal a unique mechanism for metal-dependent regulation of DNA binding specificity." Nature Structural and Molecular Biology 20, no. 6 (2013): 740-747.  Full Text
    • Schumacher, MA, Min, J, Link, TM, Guan, Z, Xu, W, Ahn, Y-H, Soderblom, EJ, Kurie, JM, Evdokimov, A, Moseley, MA, Lewis, K, and Brennan, RG. "Role of unusual P loop ejection and autophosphorylation in HipA-mediated persistence and multidrug tolerance." Cell Reports 2, no. 3 (September 20, 2012): 518-525.  Full Text
    • Amato, AA, Rajagopalan, S, Lin, JZ, Carvalho, BM, Figueira, ACM, Lu, J, Ayers, SD, Mottin, M, Silveira, RL, Souza, PCT, Mourão, RHV, Saad, MJA, Togashi, M, Simeoni, LA, Abdalla, DSP, Skaf, MS, Polikparpov, I, Lima, MCA, Galdino, SL, Brennan, RG, Baxter, JD, Pitta, IR, Webb, P, Phillips, KJ, and Neves, FAR. "GQ-16, a novel peroxisome proliferator-activated receptor γ (PPARγ) ligand, promotes insulin sensitization without weight gain." The Journal of Biological Chemistry 287, no. 33 (August 2012): 28169-28179.  Full Text
    • Hansen, S, Vulić, M, Min, J, Yen, T-J, Schumacher, MA, Brennan, RG, and Lewis, K. "Regulation of the Escherichia coli HipBA toxin-antitoxin system by proteolysis." Plos One 7, no. 6 (January 2012): e39185-null.  Full Text
    • Horstmann, N, Orans, J, Valentin-Hansen, P, Shelburne, SA, and Brennan, RG. "Structural mechanism of Staphylococcus aureus Hfq binding to an RNA A-tract." Nucleic Acids Research 40, no. 21 (2012): 11023-11035.  Full Text
    • Le, TBK, Schumacher, MA, Lawson, DM, Brennan, RG, and Buttner, MJ. "The crystal structure of the TetR family transcriptional repressor SimR bound to DNA and the role of a flexible N-terminal extension in minor groove binding." Nucleic Acids Research 39, no. 21 (November 2011): 9433-9447.  Full Text
    • Horstmann, N, Sahasrabhojane, P, Suber, B, Kumaraswami, M, Olsen, RJ, Flores, A, Musser, JM, Brennan, RG, and Shelburne, SA. "Distinct single amino acid replacements in the control of virulence regulator protein differentially impact streptococcal pathogenesis." PLoS Pathogens 7, no. 10 (2011).  Full Text
    • III, SAS, Sahasrobhajane, P, Suber, B, Keith, DB, Davenport, MT, Horstmann, N, Kumaraswami, M, Olsen, RJ, Brennan, RG, and Musser, JM. "Niche-specific contribution to streptococcal virulence of a MalR-regulated carbohydrate binding protein." Molecular Microbiology 81, no. 2 (2011): 500-514.  Full Text
    • Peters, KM, Brooks, BE, Schumacher, MA, Skurray, RA, Brennan, RG, and Brown, MH. "A single acidic residue can guide binding site selection but does not govern QaCR cationic-drug affinity." PLoS ONE 6, no. 1 (2011).  Full Text
    • Schumacher, MA, Sprehe, M, Bartholomae, M, Hillen, W, and Brennan, RG. "Structures of carbon catabolite protein A-(HPr-Ser46-P) bound to diverse catabolite response element sites reveal the basis for high-affinity binding to degenerate DNA operators." Nucleic Acids Research 39, no. 7 (2011): 2931-2942.  Full Text
    • Olsen, AS, Møller-Jensen, J, Brennan, RG, and Valentin-Hansen, P. "C-terminally truncated derivatives of Escherichia coli Hfq are proficient in riboregulation." Journal of Molecular Biology 404, no. 2 (November 2010): 173-182.  Full Text
    • Kumaraswami, M, Newberry, KJ, and Brennan, RG. "Conformational plasticity of the coiled-coil domain of BmrR is required for bmr operator binding: the structure of unliganded BmrR." Journal of Molecular Biology 398, no. 2 (April 2010): 264-275.  Full Text
    • Cho, K, Vaught, TG, Ji, H, Gu, D, Papasakelariou-Yared, C, Horstmann, N, Jennings, JM, Lee, M, Sevilla, LM, Kloc, M, Reynolds, AB, Watt, FM, Brennan, RG, Kowalczyk, AP, and McCrea, PD. "Xenopus Kazrin interacts with ARVCF-catenin, spectrin and p190B RhoGAP, and modulates RhoA activity and epithelial integrity." Journal of Cell Science 123, no. 23 (2010): 4128-4144.  Full Text
    • Nakano, MM, Lin, A, Zuber, CS, Newberry, KJ, Brennan, RG, and Zuber, P. "Promoter recognition by a complex of Spx and the C-terminal domain of the RNA polymerase α subunit." PLoS ONE 5, no. 1 (2010).  Full Text
    • Nakano, MM, Lin, A, Zuber, CS, Newberry, KJ, Brennan, RG, and Zuber, P. "Promoter recognition by a complex of Spx and the C-terminal domain of the RNA polymerase α subunit." PLoS ONE 5, no. 1 (2010).  Full Text
    • Shelburne, SA, Olsen, RJ, Suber, B, Sahasrabhojane, P, Sumby, P, Brennan, RG, and Musser, JM. "A combination of independent transcriptional regulators shapes bacterial virulence gene expression during infection." PLoS Pathogens 6, no. 3 (2010).  Full Text
    • Link, TM, Valentin-Hansen, P, and Brennan, RG. "Structure of Escherichia coli Hfq bound to polyriboadenylate RNA." Proceedings of the National Academy of Sciences 106, no. 46 (November 17, 2009): 19292-19297.  Full Text
    • "Correction for Shelburne et al., A direct link between carbohydrate utilization and virulence in the major human pathogen group A Streptococcus." Proceedings of the National Academy of Sciences 106, no. 21 (May 26, 2009): 8790-8790.  Full Text
    • Hassan, KA, Xu, Z, Watkins, RE, Brennan, RG, Skurray, RA, and Brown, MH. "Optimized production and analysis of the staphylococcal multidrug efflux protein QacA." Protein Expression and Purification 64, no. 2 (2009): 118-124.  Full Text
    • Kumaraswami, M, Schuman, JT, Seo, SM, Kaatz, GW, and Brennan, RG. "Structural and biochemical characterization of MepR, a multidrug binding transcription regulator of the Staphylococcus aureus multidrug efflux pump MepA." Nucleic Acids Research 37, no. 4 (2009): 1211-1224.  Full Text
    • Schumacher, MA, Piro, KM, Xu, W, Hansen, S, Lewis, K, and Brennan, RG. "Molecular mechanisms of HipA-mediated multidrug tolerance and its neutralization by HipB." Science 323, no. 5912 (2009): 396-401.  Full Text
    • Stoll, KE, Draper, WE, Kliegman, JI, Golynskiy, MV, Brew-Appiah, RAT, Phillips, RK, Brown, HK, Breyer, WA, Jakubovics, NS, Jenkinson, HF, Brennan, RG, Cohen, SM, and Glasfeld, A. "Characterization and structure of the manganese-responsive transcriptional regulator ScaR." Biochemistry 48, no. 43 (2009): 10308-10320.  Full Text
    • Newberry, KJ, Huffman, JL, Miller, MC, Vazquez-Laslop, N, Neyfakh, AA, and Brennan, RG. "Structures of BmrR-drug complexes reveal a rigid multidrug binding pocket and transcription activation through tyrosine expulsion." The Journal of Biological Chemistry 283, no. 39 (September 2008): 26795-26804.  Full Text
    • Amen, M, Espinoza, HM, Cox, C, Liang, X, Wang, J, Link, TME, Brennan, RG, Martin, J, and Amendt, BA. "Chromatin-associated HMG-17 is a major regulator of homeodomain transcription factor activity modulated by Wnt/β-catenin signaling." Nucleic Acids Research 36, no. 2 (2008): 462-476.  Full Text
    • III, SAS, Keith, D, Horstmann, N, Sumby, P, Davenport, MT, Graviss, EA, Brennan, RG, and Musser, JM. "A direct link between carbohydrate utilization and virulence in the major human pathogen group A Streptococcus." Proceedings of the National Academy of Sciences of the United States of America 105, no. 5 (2008): 1698-1703.  Full Text
    • III, SAS, Keith, DB, Davenport, MT, Horstmann, N, Brennan, RG, and Musser, JM. "Molecular characterization of group A Streptococcus maltodextrin catabolism and its role in pharyngitis." Molecular Microbiology 69, no. 2 (2008): 436-452.  Full Text
    • Peters, KM, Schuman, JT, Skurray, RA, Brown, MH, Brennan, RG, and Schumacher, MA. "QacR-cation recognition is mediated by a redundancy of residues capable of charge neutralization." Biochemistry 47, no. 31 (2008): 8122-8129.  Full Text
    • Brennan, RG, and Link, TM. "Hfq structure, function and ligand binding." Current Opinion in Microbiology 10, no. 2 (April 2007): 125-133. (Review)  Full Text
    • Brooks, BE, Piro, KM, and Brennan, RG. "Multidrug-binding transcription factor QacR binds the bivalent aromatic diamidines DB75 and DB359 in multiple positions." Journal of the American Chemical Society 129, no. 26 (2007): 8389-8395.  Full Text
    • DeWitt, MA, Kliegman, JI, Helmann, JD, Brennan, RG, Farrens, DL, and Glasfeld, A. "The Conformations of the Manganese Transport Regulator of Bacillus subtilis in its Metal-free State." Journal of Molecular Biology 365, no. 5 (2007): 1257-1265.  Full Text
    • Newberry, KJ, Fuangthong, M, Panmanee, W, Mongkolsuk, S, and Brennan, RG. "Structural Mechanism of Organic Hydroperoxide Induction of the Transcription Regulator OhrR." Molecular Cell 28, no. 4 (2007): 652-664.  Full Text
    • Schumacher, MA, Seidel, G, Hillen, W, and Brennan, RG. "Structural Mechanism for the Fine-tuning of CcpA Function by The Small Molecule Effectors Glucose 6-Phosphate and Fructose 1,6-Bisphosphate." Journal of Molecular Biology 368, no. 4 (2007): 1042-1050.  Full Text
    • Kliegman, JI, Griner, SL, Helmann, JD, Brennan, RG, and Glasfeld, A. "Structural basis for the metal-selective activation of the manganese transport regulator of Bacillus subtilis." Biochemistry 45, no. 11 (2006): 3493-3505.  Full Text
    • Mattison, K, Wilbur, JS, So, M, and Brennan, RG. "Structure of FitAB from Neisseria gonorrhoeae bound to DNA reveals a tetramer of toxin-antitoxin heterodimers containing pin domains and ribbon-helix-helix motifs." Journal of Biological Chemistry 281, no. 49 (2006): 37942-37951.  Full Text
    • Schumacher, MA, Seidel, G, Hillen, W, and Brennan, RG. "Phosphoprotein Crh-Ser⁴⁶-P displays altered binding to CcpA to effect carbon catabolite regulation." Journal of Biological Chemistry 281, no. 10 (2006): 6793-6800.  Full Text
    • Newberry, KJ, Nakano, S, Zuber, P, and Brennan, RG. "Crystal structure of the Bacillus subtilis anti-alpha, global transcriptional regulator, Spx, in complex with the alpha C-terminal domain of RNA polymerase." Proceedings of the National Academy of Sciences of the United States of America 102, no. 44 (November 2005): 15839-15844.  Full Text
    • Hong, M, Fuangthong, M, Helmann, JD, and Brennan, RG. "Structure of an OhrR-ohrA operator complex reveals the DNA binding mechanism of the MarR family." Molecular Cell 20, no. 1 (October 2005): 131-141.  Full Text
    • Hoffmann, KM, Williams, D, Shafer, WM, and Brennan, RG. "Characterization of the multiple transferable resistance repressor, MtrR, from Neisseria gonorrhoeae." Journal of Bacteriology 187, no. 14 (July 2005): 5008-5012.  Full Text
    • Ramos, JL, Martínez-Bueno, M, Molina-Henares, AJ, Terán, W, Watanabe, K, Zhang, X, Gallegos, MT, Brennan, R, and Tobes, R. "The TetR family of transcriptional repressors." Microbiology and Molecular Biology Reviews : Mmbr 69, no. 2 (June 2005): 326-356. (Review)  Full Text
    • Wilbur, JS, Chivers, PT, Mattison, K, Potter, L, Brennan, RG, and So, M. "Neisseria gonorrhoeae FitA interacts with FitB to bind DNA through its ribbon-helix-helix motif." Biochemistry 44, no. 37 (2005): 12515-12524.  Full Text
    • Schumacher, MA, Miller, MC, and Brennan, RG. "Structural mechanism of the simultaneous binding of two drugs to a multidrug-binding protein." The Embo Journal 23, no. 15 (August 2004): 2923-2930.  Full Text
    • Newberry, KJ, and Brennan, RG. "The structural mechanism for transcription activation by MerR family member multidrug transporter activation, N terminus." The Journal of Biological Chemistry 279, no. 19 (May 2004): 20356-20362.  Full Text
    • Murray, DS, Schumacher, MA, and Brennan, RG. "Crystal Structures of QacR-Diamidine Complexes Reveal Additional Multidrug-binding Modes and A Novel Mechanism of Drug Charge Neutralization." Journal of Biological Chemistry 279, no. 14 (2004): 14365-14371.  Full Text
    • Schumacher, MA, Allen, GS, Diel, M, Seidel, G, Hillen, W, and Brennan, RG. "Structural basis for allosteric control of the transcription regulator CcpA by the phosphoprotein HPr-Ser46-P." Cell 118, no. 6 (2004): 731-741.  Full Text
    • Allen, GS, Steinhauer, K, Hillen, W, Stülke, J, and Brennan, RG. "Crystal structure of HPr kinase/phosphatase from Mycoplasma pneumoniae." Journal of Molecular Biology 326, no. 4 (2003): 1203-1217.  Full Text
    • Glasfeld, A, Guedon, E, Helmann, JD, and Brennan, RG. "Structure of the manganese-bound manganese transport regulator of Bacillus subtilis." Nature Structural Biology 10, no. 8 (2003): 652-657.  Full Text
    • Schumacher, MA, and Brennan, RG. "Deciphering the molecular basis of multidrug recognition: Crystal structures of the Staphylococcus aureus multidrug binding transcription regulator QacR." Research in Microbiology 154, no. 2 (2003): 69-77.  Full Text
    • Schumacher, MA, Pearson, RF, Møller, T, Valentin-Hansen, P, and Brennan, RG. "Structures of the pleiotropic translational regulator Hfq and an Hfq-RNA complex: a bacterial Sm-like protein." The Embo Journal 21, no. 13 (July 2002): 3546-3556.  Full Text
    • "Erratum." The Embo Journal 21, no. 9 (May 1, 2002): 2301-2301.  Full Text
    • Steinhauer, K, Allen, GS, Hillen, W, Stülke, J, and Brennan, RG. "Crystallization, preliminary X-ray analysis and biophysical characterization of HPr kinase/phosphatase of Mycoplasma pneumoniae." Acta Crystallographica Section D: Biological Crystallography 58, no. Pt 3 (March 2002): 515-518.  Full Text
    • Huffman, JL, and Brennan, RG. "Prokaryotic transcription regulators: more than just the helix-turn-helix motif." Current Opinion in Structural Biology 12, no. 1 (February 2002): 98-106. (Review)  Full Text
    • Bashor, C, Denu, JM, Brennan, RG, and Ullman, B. "Kinetic mechanism of adenine phosphoribosyltransferase from Leishmania donovani." Biochemistry 41, no. 12 (2002): 4020-4031.  Full Text
    • Godsey, MH, Heldwein, EEZ, and Brennan, RG. "Structural biology of bacterial multidrug resistance gene regulators." Journal of Biological Chemistry 277, no. 43 (2002): 40169-40172.  Full Text
    • Huffman, JL, Lu, F, Zalkin, H, and Brennan, RG. "Role of residue 147 in the gene regulatory function of the Escherichia coli purine repressor." Biochemistry 41, no. 2 (2002): 511-520.  Full Text
    • Møller, T, Franch, T, Højrup, P, Keene, DR, Bächinger, HP, Brennan, RG, and Valentin-Hansen, P. "Hfq: a bacterial Sm-like protein that mediates RNA-RNA interaction." Molecular Cell 9, no. 1 (January 2002): 23-30.  Full Text
    • Schumacher, MA, Bashor, CJ, Minsun, HS, Otsu, K, Zhu, S, Parry, RJ, Ullman, B, and Brennan, RG. "The structural mechanism of GTP stabilized oligomerization and catalytic activation of the Toxoplasma gondii uracil phosphoribosyltransferase." Proceedings of the National Academy of Sciences of the United States of America 99, no. 1 (2002): 78-83.  Full Text
    • Schumacher, MA, Miller, MC, Grkovic, S, Brown, MH, Skurray, RA, and Brennan, RG. "Structural basis for cooperative DNA binding by two dimers of the multidrug-binding protein QacR." EMBO Journal 21, no. 5 (2002): 1210-1218.  Full Text
    • Schumacher, MA, and Brennan, RG. "Structural mechanisms of multidrug recognition and regulation by bacterial multidrug transcription factors." Molecular Microbiology 45, no. 4 (2002): 885-893.  Full Text
    • Grkovic, S, Brown, MH, Schumacher, MA, Brennan, RG, and Skurray, RA. "The staphylococcal QacR multidrug regulator binds a correctly spaced operator as a pair of dimers." Journal of Bacteriology 183, no. 24 (December 2001): 7102-7109.  Full Text
    • Schumacher, MA, Miller, MC, Grkovic, S, Brown, MH, Skurray, RA, and Brennan, RG. "Structural mechanisms of QacR induction and multidrug recognition." Science (New York, N.Y.) 294, no. 5549 (December 2001): 2158-2163.  Full Text
    • Brennan, RG. "Introduction: multidrug resistance." Seminars in Cell & Developmental Biology 12, no. 3 (June 2001): 201-204.  Full Text
    • Craig, JC, Schumacher, MA, Mansoor, SE, Farrens, DL, Brennan, RG, and Goodman, RH. "Consensus and variant cAMP-regulated enhancers have distinct CREB-binding properties." The Journal of Biological Chemistry 276, no. 15 (April 2001): 11719-11728.  Full Text
    • Godsey, MH, Baranova, NN, Neyfakh, AA, and Brennan, RG. "Crystal Structure of MtaN, a Global Multidrug Transporter Gene Activator." Journal of Biological Chemistry 276, no. 50 (2001): 47178-47184.  Full Text
    • Heldwein, EE, and Brennan, RG. "Crystal structure of the transcription activator BmrR bound to DNA and a drug." Nature 409, no. 6818 (January 2001): 378-382.  Full Text
    • Huffman, JL, Mokashi, A, Bächinger, HP, and Brennan, RG. "The Basic Helix-Loop-Helix Domain of the Aryl Hydrocarbon Receptor Nuclear Transporter (ARNT) Can Oligomerize and Bind E-box DNA Specifically." Journal of Biological Chemistry 276, no. 44 (2001): 40537-40544.  Full Text
    • Schumacher, MA, Hurlburt, BK, and Brennan, RG. "Erratum: Crystal structures of SarA, a pleiotropic regulator of virulence genes in S. aureus (Nature (20010 409 (215-219))." Nature 414, no. 6859 (2001): 85--.
    • Schumacher, MA, Hurlburt, BK, and Brennan, RG. "Crystal structures of SarA, a pleiotropic regulator of virulence genes in S. aureus." Nature 409, no. 6817 (January 2001): 215-219.  Full Text
    • Godsey, MH, Baranova, NN, Neyfakh, AA, and Brennan, RG. "Crystallization and preliminary X-ray diffraction studies on the DNA-binding domain of the multidrug transporter activation protein (MtaN) from Bacillus subtilis." Acta Crystallographica Section D: Biological Crystallography 56, no. Pt 11 (November 2000): 1456-1458.  Full Text
    • Brown, M, Schumacher, MA, Wiens, GD, Brennan, RG, and Rittenberg, MB. "The structural basis of repertoire shift in an immune response to phosphocholine." The Journal of Experimental Medicine 191, no. 12 (June 2000): 2101-2112.  Full Text
    • Schumacher, MA, Scott, DM, Mathews, II, Ealick, SE, Roos, DS, Ullman, B, and Brennan, RG. "Crystal structures of Toxoplasma gondii adenosine kinase reveal a novel catalytic mechanism and prodrug binding." Journal of Molecular Biology 298, no. 5 (May 2000): 875-893.  Full Text
    • Cardinaux, JR, Notis, JC, Zhang, Q, Vo, N, Craig, JC, Fass, DM, Brennan, RG, and Goodman, RH. "Recruitment of CREB binding protein is sufficient for CREB-mediated gene activation." Molecular and Cellular Biology 20, no. 5 (March 2000): 1546-1552.  Full Text
    • Schumacher, MA, Scott, DM, Mathews, II, Ealick, SE, Roos, DS, Ullman, B, and Brennan, RG. "Crystal structures of Toxoplasma gondii adenosine kinase reveal a novel catalytic mechanism and prodrug binding." Journal of Molecular Biology 296, no. 2 (February 2000): 549-567.  Full Text
    • Zheleznova, EE, Markham, P, Edgar, R, Bibi, E, Neyfakh, AA, and Brennan, RG. "A structure-based mechanism for drug binding by multidrug transporters." Trends in Biochemical Sciences 25, no. 2 (February 2000): 39-43. (Review)  Full Text
    • Schumacher, MA, Goodman, RH, and Brennan, RG. "The structure of a CREB bZIP·somatostatin CRE complex reveals the basis for selective dimerization and divalent cation-enhanced DNA binding." Journal of Biological Chemistry 275, no. 45 (2000): 35242-35247.  Full Text
    • Vazquez-Laslop, N, Zheleznovat, EE, Markham, PN, Brennan, RG, and Neyfakh, AA. "Recognition of multiple drugs by a single protein: A trivial solution of an old paradox." Biochemical Society Transactions 28, no. 4 (2000): 517-520.
    • Zheleznova, EE, Crosa, JH, and Brennan, RG. "Characterization of the DNA- and metal-binding properties of Vibrio anguillarum Fur reveals conservation of a structural Zn²⁺ ion." Journal of Bacteriology 182, no. 21 (2000): 6264-6267.  Full Text
    • Rechtin, TM, Gillaspy, AF, Schumacher, MA, Brennan, RG, Smeltzer, MS, and Hurlburt, BK. "Characterization of the SarA virulence gene regulator of Staphylococcus aureus." Molecular Microbiology 33, no. 2 (July 1999): 307-316.  Full Text
    • Glasfeld, A, Koehler, AN, Schumacher, MA, and Brennan, RG. "The role of lysine 55 in determining the specificity of the purine repressor for its operators through minor groove interactions." Journal of Molecular Biology 291, no. 2 (1999): 347-361.  Full Text
    • Phillips, CL, Ullman, B, Brennan, RG, and Hill, CP. "Crystal structures of adenine phosphoribosyltransferase from Leishmania donovani." EMBO Journal 18, no. 13 (1999): 3533-3545.  Full Text
    • Zheleznova, EE, Markham, PN, Neyfakh, AA, and Brennan, RG. "Structural basis of multidrug recognition by BMRR, a transcription activator of a multidrug transporter." Cell 96, no. 3 (1999): 353-362.  Full Text
    • Schumacher, MA, Carter, D, Scott, DM, Roos, DS, Ullman, B, and Brennan, RG. "Crystal structures of Toxoplasma gondii uracil phosphoribosyltransferase reveal the atomic basis of pyrimidine discrimination and prodrug binding." The Embo Journal 17, no. 12 (June 1998): 3219-3232.  Full Text
    • Arvidson, DN, Lu, F, Faber, C, Zalkin, H, and Brennan, RG. "The structure of PurR mutant L54M shows an alternative to DNA kinking." Nature Structural Biology 5, no. 6 (1998): 436-441.  Full Text
    • Lu, F, Brennan, RG, and Zalkin, H. "Escherichia coli purine repressor: Key residues for the allosteric transition between active and inactive conformations and for interdomain signaling." Biochemistry 37, no. 45 (1998): 15680-15690.  Full Text
    • Lu, F, Schumacher, MA, Arvidson, DN, Haldimann, A, Wanner, BL, Zalkin, H, and Brennan, RG. "Structure-based redesign of corepressor specificity of the Escherichia coli purine repressor by substitution of residue 190." Biochemistry 37, no. 4 (1998): 971-982.  Full Text
    • Lundblad, JR, Kwok, RPS, Laurance, ME, Huang, MS, Richards, JP, Brennan, RG, and Goodman, RH. "The human T-cell leukemia virus-1 transcriptional activator tax enhances cAMP-responsive element-binding protein (CREB) binding activity through interactions with the DNA minor groove." Journal of Biological Chemistry 273, no. 30 (1998): 19251-19259.  Full Text
    • Schumacher, MA, Glasfeld, A, Zalkin, H, and Brennan, RG. "The x-ray structure of the PurR-guanine-purF operator complex reveals the contributions of complementary electrostatic surfaces and a water- mediated hydrogen bond to corepressor specificity and binding affinity." Journal of Biological Chemistry 272, no. 36 (1997): 22648-22653.  Full Text
    • Schumacher, MA, Zheleznova, EE, Poundstone, KS, Kluger, R, Jones, RT, and Brennan, RG. "Allosteric intermediates indicate R2 is the liganded hemoglobin end state." Proceedings of the National Academy of Sciences of the United States of America 94, no. 15 (1997): 7841-7844.  Full Text
    • Zheleznova, EE, Markham, PN, Neyfakh, AA, and Brennan, RG. "Preliminary structural studies on the multi-ligand-binding domain of the transcription activator, BmrR, from Bacillus subtilis." Protein Science 6, no. 11 (1997): 2465-2468.  Full Text
    • Schumacher, MA, Carter, D, Roos, DS, Ullman, B, and Brennan, RG. "Crystal structures of Toxoplasma gondii HGXPRTase reveal the catalytic role of a long flexible loop." Nature Structural Biology 3, no. 10 (October 1996): 881-887.  Full Text
    • Phillíps, CL, Ullman, B, and Brennan, RG. "Crystallization of the purine salvage enzyme adenine phosphoribosyltransferase." Proteins 25, no. 4 (August 1996): 510-513.  Full Text
    • Lewis, M, Chang, G, Horton, NC, Kercher, MA, Pace, HC, Schumacher, MA, Brennan, RG, and Lu, P. "Crystal structure of the lactose operon repressor and its complexes with DNA and inducer." Science (New York, N.Y.) 271, no. 5253 (March 1996): 1247-1254.  Full Text
    • Glasfeld, A, Schumacher, MA, Choi, K-Y, Zalkin, H, and Brennan, RG. "A Positively Charged Residue Bound in the Minor Groove Does Not Alter the Bending of a DNA Duplex." Journal of the American Chemical Society 118, no. 51 (January 1996): 13073-13074.  Full Text
    • Phillips, CL, Ullman, B, and Brennan, RG. "Crystallization of the purine salvage enzyme adenine phosphoribosyltransferase." Proteins: Structure, Function and Genetics 25, no. 4 (1996): 510-513.  Full Text
    • Richards, JP, Bächinger, HP, Goodman, RH, and Brennan, RG. "Analysis of the structural properties of cAMP-responsive element-binding protein (CREB) and phosphorylated CREB." Journal of Biological Chemistry 271, no. 23 (1996): 13716-13723.  Full Text
    • Nagadoi, A, Morikawa, S, Nakamura, H, Enari, M, Kobayashi, K, Yamamoto, H, Sampei, G, Mizobuchi, K, Schumacher, MA, Brennan, RG, and Nishimura, Y. "Structural comparison of the free and DNA-bound forms of the purine repressor DNA-binding domain." Structure 3, no. 11 (1995): 1217-1224.
    • Nagadoi, A, Nakazawa, K, Morikawa, S, Nakamura, H, Sampei, G, Mizobuchi, K, Yamamoto, H, Schumacher, MA, Brennan, RG, and Nishimura, Y. "Conformational changes of purine repressor DNA-binding domain upon complexation with DNA." Nucleic acids symposium series , no. 34 (1995): 63-64.
    • Schumacher, MA, Choi, KY, Lu, F, Zalkin, H, and Brennan, RG. "Mechanism of corepressor-mediated specific DNA binding by the purine repressor." Cell 83, no. 1 (1995): 147-155.
    • Schumacher, MA, Dixon, MM, Kluger, R, Jones, RT, and Brennan, RG. "Allosteric transition intermediates modelled by crosslinked haemoglobins." Nature 375, no. 6526 (1995): 84-87.
    • Brickey, DA, Bann, JG, Fong, Y-L, Perrino, L, Brennan, RG, and Soderling, TR. "Mutational analysis of the autoinhibitory domain of calmodulin kinase II." Journal of Biological Chemistry 269, no. 46 (1994): 29047-29054.
    • Kwok, RPS, Lundblad, JR, Chrivia, JC, Richards, JP, Bächinger, HP, Brennan, RG, Roberts, SGE, Green, MR, and Goodman, RH. "Nuclear protein CBP is a coactivator for the transcription factor CREB." Nature 370, no. 6486 (1994): 223-226.  Full Text
    • Loriaux, MM, Brennan, RG, and Goodman, RH. "Modulatory function of CREB·CREMα heterodimers depends upon CREMα phosphorylation." Journal of Biological Chemistry 269, no. 46 (1994): 28839-28843.
    • Schumacher, MA, Choi, KY, Zalkin, H, and Brennan, RG. "Crystallization and preliminary X-ray analysis of an Escherichia coli purine repressor-hypoxanthine-DNA complex." Journal of Molecular Biology 242, no. 3 (1994): 302-305.  Full Text
    • Schumacher, MA, Choi, KY, Zalkin, H, and Brennan, RG. "Crystal structure of lacl member, PurR, bound to DNA: Minor groove binding by α helices." Science 266, no. 5186 (1994): 763-770.
    • Brennan, RG. "The winged-helix DNA-binding motif: Another helix-turn-helix takeoff." Cell 74, no. 5 (1993): 773-776.  Full Text
    • Loriaux, MM, Rehfuss, RP, Brennan, RG, and Goodman, RH. "Engineered leucine zippers show that hemiphosphorylated CREB complexes are transcriptionally active." Proceedings of the National Academy of Sciences of the United States of America 90, no. 19 (1993): 9046-9050.  Full Text
    • Schumacher, MA, Macdonald, JR, Björkman, J, Mowbray, SL, and Brennan, RG. "Structural analysis of the purine repressor, an Escherichia coli DNA-binding protein." Journal of Biological Chemistry 268, no. 17 (1993): 12282-12288.
    • Spiro, C, Richards, JP, Chandrasekaran, S, Brennan, RG, and Mcmurray, CT. "Secondary structure creates mismatched base pairs required for high-affinity binding of cAMP response element-binding protein to the human enkephalin enhancer." Proceedings of the National Academy of Sciences of the United States of America 90, no. 10 (1993): 4606-4610.
    • Wilson, KP, Shewchuk, LM, Brennan, RG, Otsuka, AJ, and Matthews, BW. "Escherichia coli biotin holoenzyme synthetase/bio repressor crystal structure delineates the biotin- and DNA-binding domains." Proceedings of the National Academy of Sciences of the United States of America 89, no. 19 (October 1992): 9257-9261.  Full Text
    • Brennan, RG. "DNA recognition by the helix-turn-helix motif." Current Opinion in Structural Biology 2, no. 1 (February 1992): 100-108.  Full Text
    • Schumacher, MA, Choi, KY, Zalkin, H, and Brennan, RG. "Crystallization and preliminary X-ray studies on the co-repressor binding domain of the Escherichia coli purine repressor." Journal of Molecular Biology 225, no. 4 (1992): 1131-1133.  Full Text
    • Carr, DW, Stofko-Hahn, RE, Fraser, ID, Bishop, SM, Acott, TS, Brennan, RG, and Scott, JD. "Interaction of the regulatory subunit (RII) of cAMP-dependent protein kinase with RII-anchoring proteins occurs through an amphipathic helix binding motif." The Journal of Biological Chemistry 266, no. 22 (August 1991): 14188-14192.
    • Brennan, RG. "Interactions of the helix-turn-helix binding domain." Current Opinion in Structural Biology 1, no. 1 (February 1991): 80-88.  Full Text
    • Brennan, RG. "Interactions of the helix-turn-helix binding domain." Current Opinion in Structural Biology 1, no. 1 (1991): 80-88.
    • Dixon, MM, Brennan, RG, and Matthews, BW. "Structure of γ-chymotrypsin in the range pH 2.0 to pH 10.5 suggests that γ-chymotrypsin is a covalent acyl-enzyme adduct at low pH." International Journal of Biological Macromolecules 13, no. 2 (1991): 89-96.  Full Text
    • Brennan, RG, Roderick, SL, Takeda, Y, and Matthews, BW. "Protein-DNA conformational changes in the crystal structure of a λ Cro-operator complex." Proceedings of the National Academy of Sciences of the United States of America 87, no. 20 (1990): 8165-8169.
    • Brennan, RG, Vasu, S, Matthews, BW, and Otsuka, AJ. "Crystallization of the bifunctional biotin operon repressor." Journal of Biological Chemistry 264, no. 1 (1989): 5--.
    • Brennan, RG, and Matthews, BW. "The helix-turn-helix DNA binding motif." Journal of Biological Chemistry 264, no. 4 (1989): 1903-1906.
    • Brennan, RG, and Matthews, BW. "Structural basis of DNA-protein recognition." Trends in Biochemical Sciences 14, no. 7 (1989): 286-290.
    • Brennan, RG, Wozniak, J, Faber, R, and Matthews, BW. "Crystallization of mutant lysozymes from bacteriophage T4." Journal of Crystal Growth 90, no. 1-3 (July 1988): 160-167.  Full Text
    • Brennan, RG, Kim, J, Takeda, Y, Anderson, WF, and Matthews, BW. "Studies on the crystallization of the cro protein-pseudo OR3 complex." Journal of Crystal Growth 76, no. 3 (August 1986): 715-718.  Full Text
    • Brennan, RG, Pyzalska, D, Blonski, WJP, Hruska, FE, and Sundaralingam, M. "Crystal structure of the promutagen O⁴-methylthymidine: Importance of the anti conformation of the O(4) methoxy group and possible mispairing of O⁴-methylthymidine with guanine." Biochemistry 25, no. 5 (1986): 1181-1185.
    • Brennan, RG, Takeda, Y, Kim, J, Anderson, WF, and Matthews, BW. "Crystallization of a complex of cro repressor with a 17 base-pair operator." Journal of Molecular Biology 188, no. 1 (1986): 115-118.
    • Brennan, RG, Westhof, E, and Sundaralingam, M. "Structure of a Z-DNA with two different backbone chain conformations. Stabilization of the decadeoxyoligonucleotide d(CGTACGTACG) by [Co(NH₃)₆]³⁺ binding to the guanine." Journal of Biomolecular Structure and Dynamics 3, no. 4 (1986): 649-665.
    • Brennan, RG, and Sundaralingam, M. "Crystallization and preliminary crystallographic studies of the decadeoxyoligonucleotide d(CpGpTpApCpGpTpApCpG)." Journal of Molecular Biology 181, no. 4 (1985): 561-563.
    • Brennan, RG, Kondo, NS, and Sundaralingam, M. "Crystal structure and conformation of the phosphotriester adenosine 5'-O-(diethyl phosphate). Possible steric and conformational mechanisms for the biochemical and biological effects arising from phosphate alkylation." Journal of the American Chemical Society 106, no. 19 (September 1984): 5671-5676.  Full Text
    • Brennan, RG, Kondo, NS, and Sundaralingam, M. "X-ray structure of cytidine-5′-O-dimethylphosphate. Novel stacking between the ribosyl O(2′) hydroxyl oxygen atom and the base." Nucleic Acids Research 12, no. 17 (1984): 6813-6825.  Full Text
    • Brennan, RG, Prive, GG, Blonski, WJP, Hruska, FE, and Sundaralingam, M. "Molecular and crystal structure of O4-methyluridine. Reaction coordinates for an incipient nucleophilic attack seen by short intermolecular sugar-base interactions." Journal of the American Chemical Society 105, no. 26 (December 1983): 7737-7742.  Full Text
    • Brennan, RG, Prive, GG, and Blonski, WJP. "Crystal structure of O⁴-methyl uridine: Stacking induced changes in the geometry of the pyrimidine ring and its mutagenic role." Journal of Biomolecular Structure and Dynamics 1, no. 4 (1983): 939-945.
    • Wells, RD, Brennan, R, Chapman, KA, Goodman, TC, Hart, PA, Hillen, W, Kellogg, DR, Kilpatrick, MW, Klein, RD, Klysik, J, Lambert, PF, Larson, JE, Miglietta, JJ, Neuendorf, SK, O'Connor, TR, Singleton, CK, Stirdivant, SM, Veneziale, CM, Wartell, RM, and Zacharias, W. "Left-handed DNA helices, supercoiling, and the B-Z junction." Cold Spring Harbor symposia on quantitative biology 47 Pt 1 (January 1983): 77-84.  Full Text
    • Wells, RD, Brennan, R, Chapman, KA, Goodman, TC, Hart, PA, Hillen, W, Kellogg, DR, Kilpatrick, MW, Klein, RD, Klysik, J, Lambert, PF, Larson, JE, Miglietta, JJ, Neuendorf, SK, O'Connor, TR, Singleton, CK, Stirdivant, SM, Veneziale, CM, Wartell, RM, and Zacharias, W. "Left-handed DNA Helices, Supercoiling, and the B-Z Junction." Cold Spring Harbor Symposia on Quantitative Biology 47, no. 0 (January 1, 1983): 77-84.  Full Text
    • Sundaralingam, M, Brennan, RG, Swaminathan, P, Haromy, TP, Drendel, WB, Bemiller, JN, Chmielewski, M, and Cerretti, DP. "Occurrence of the Unusual2C5(1C4) Chair Conformation in Two Carbohydrates and the Reverse Anomeric Effect. X-Ray Structures of 3,4,5,7-Tetra-O-Acetyl-2,6-Anhydro-D-Glycero-D-Ido-Heptonamide (1) and 3,4,5,7-Tetra-O-Acetyl-2,6-Anhydro-D-Glycero-L-Gluco-Heptonamide (2)." Journal of Carbohydrate Chemistry 1, no. 1 (January 1982): 85-103.  Full Text
    • Holtz, A, Brennan, RG, Battista, D, and Terner, C. "Androgen control of an inhibitory modulator of phosphodiesterase in rat epididymis and prostate." Endocrinology 108, no. 4 (1981): 1538-1544.
    • Terner, C, Holtz, A, Brennan, RG, and Battista, D. "Androgen control of phosphodiesterase modulator in epididymis and prostate of rat." Federation Proceedings 39, no. 3 II (1980): 3765--.

  • Conference Papers

    • Birukou, I, Kaatz, GW, and Brennan, RG. "Crystal Structure of a MepR-DNA Complex Reveals the Mechanism of Transcription Repression of S. Aureus Multidrug Efflux Pump mepA." January 29, 2013.  Full Text  Link to Item
• Teaching & Mentoring
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  Recent Courses

  • BIOCHEM 393: Research Independent Study 2019
  • BIOCHEM 593: Research Independent Study 2019
  • BIOCHEM 746S: Biochemistry Seminar 2019
  • BIOCHEM 745S: Biochemistry Seminar 2018

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