Joseph Linton Roberts | Scholars@Duke

Joseph Linton Roberts
Professor of Pediatrics

My overall research interests are studying T cell development and defining the molecular bases of inherited immunodeficiency diseases. We are using standard candidate gene analysis approaches as well as new high throughput genome-wide sequencing, bioinformatics and functional screening in zebrafish and murine models in our work. Using these strategies we have recently reported a new molecular etiology of severe combined immunodeficiency (SCID), CD3 zeta chain deficiency. In collaboration with Dr. Wesley Burks we are also examining changes in T cell transcription patterns following treatment for peanut allergy using genome wide oligonucleotide microarrays.

Current Appointments & Affiliations

Professor of Pediatrics, Pediatrics, Allergy and Immunology, Pediatrics 2017

Contact Information

Box 2898 Med Ctr, Durham, NC 27710
Room 101B MSRB1, Duke University Medical Center, Durham, NC 27710
rober060@mc.duke.edu (919) 684-6534

Background
Education, Training, & Certifications
- Research Associate, Immunology, Duke University 1994 - 1996
- Fellow, Experimental Immunology Branch, Cancer Institute, National Institutes of Health 1988 - 1994
- Ph.D., Duke University 1992
- Fellow, Pediatric Allergy/Immunology, Pediatrics, Duke University 1984 - 1988
- Internship/Residency, Pediatrics, Yale University 1981 - 1984
- M.D., Emory University 1981
Medical Licensure
- 9601704. North Carolina. 2018
Duke Appointment History
- Associate Professor of Pediatrics, Pediatrics, Allergy and Immunology, Pediatrics 2010 - 2017
- Assistant Professor of Pediatrics, Pediatrics, Allergy and Immunology, Pediatrics 2006 - 2010
- Assistant Clinical Professor of Pediatrics, Pediatrics, Allergy and Immunology, Pediatrics 2005 - 2006
- Assistant Professor of Pediatrics, Pediatrics, Allergy and Immunology, Pediatrics 1997 - 2005
Research
Selected Grants
- Sublingual Immunotherapy for Peanut Allergy awarded by National Institutes of Health 2008 - 2013
- CTSA UL awarded by National Institutes of Health 2006 - 2012
- Identification of Disease-Causing Mutations in SCID Using Exome-Wide Sequencing awarded by National Institutes of Health 2009 - 2012
- Understanding the Mechanism of Mucosal Immunotherapy awarded by National Institutes of Health 2007 - 2012
- Development And Persistence Of Immunity In SCID Chimeras awarded by National Institutes of Health 1998 - 2010
- Structure-Function Analysis of JAK3 in Human SCID awarded by National Institutes of Health 1997 - 2002
- Research Training In Allergy And Clinical Immunology awarded by National Institutes of Health 1907 - 1999
- Immune Cell Cooperation In Human Haploidentical Chimeras awarded by National Institutes of Health 1990 - 1991
Publications & Artistic Works
Selected Publications
- Conference Papers
  - Boutboul, D, Kuehn, HS, Van de Wyngaert, Z, Niemela, JE, Callebaut, I, Stoddard, J, Lenoir, C, Barlogis, V, Farnarier, C, Vely, F, Yoshida, N, Kojima, S, Kanegane, H, Hoshino, A, Hauck, F, Lhermitte, L, Asnafi, V, Roehrs, P, Chen, S, Verbsky, JW, Calvo, KR, Husami, A, Zhang, K, Roberts, J, Amrol, D, Sleaseman, J, Hsu, AP, Holland, SM, Marsh, R, Fischer, A, Fleisher, TA, Picard, C, Latour, S, and Rosenzweig, SD. "Dominant-negative IKZF1 mutations cause a T, B, and myeloid cell combined immunodeficiency." July 2018. Full Text
- Journal Articles
  - Petrovski, S, Parrott, RE, Roberts, JL, Huang, H, Yang, J, Gorentla, B, Mousallem, T, Wang, E, Armstrong, M, McHale, D, MacIver, NJ, Goldstein, DB, Zhong, X-P, and Buckley, RH. "Dominant Splice Site Mutations in PIK3R1 Cause Hyper IgM Syndrome, Lymphadenopathy and Short Stature." Journal of clinical immunology 36, no. 5 (July 2016): 462-471. Full Text Open Access Copy
  - Mousallem, T, Urban, TJ, McSweeney, KM, Kleinstein, SE, Zhu, M, Adeli, M, Parrott, RE, Roberts, JL, Krueger, B, Buckley, RH, and Goldstein, DB. "Clinical application of whole-genome sequencing in patients with primary immunodeficiency." The Journal of allergy and clinical immunology 136, no. 2 (August 2015): 476-9.e6. (Letter) Full Text
  - Mousallem, T, Yang, J, Urban, TJ, Wang, H, Adeli, M, Parrott, RE, Roberts, JL, Goldstein, DB, Buckley, RH, and Zhong, X-P. "A nonsense mutation in IKBKB causes combined immunodeficiency." Blood 124, no. 13 (September 2014): 2046-2050. Full Text
  - Roberts, JL, Buckley, RH, Luo, B, Pei, J, Lapidus, A, Peri, S, Wei, Q, Shin, J, Parrott, RE, Dunbrack, RL, Testa, JR, Zhong, X-P, and Wiest, DL. "CD45-deficient severe combined immunodeficiency caused by uniparental disomy." Proc Natl Acad Sci U S A 109, no. 26 (June 26, 2012): 10456-10461. Full Text Link to Item
  - Yu, GP, Nadeau, KC, Berk, DR, de Saint Basile, G, Lambert, N, Knapnougel, P, Roberts, J, Kavanau, K, Dunn, E, Stiehm, ER, Lewis, DB, Umetsu, DT, Puck, JM, and Cowan, MJ. "Genotype, phenotype, and outcomes of nine patients with T-B+NK+ SCID." Pediatric Transplantation 15, no. 7 (November 2011): 733-741. Full Text
  - Tracy, ET, Haas, KM, Gentry, T, Danko, M, Roberts, JL, Kurtzberg, J, and Rice, HE. "Partial splenectomy but not total splenectomy preserves immunoglobulin M memory B cells in mice." Journal of Pediatric Surgery 46, no. 9 (September 2011): 1706-1710. Full Text
  - Sarzotti-Kelsoe, M, Win, CM, Parrott, RE, Cooney, M, Moser, BK, Roberts, JL, Sempowski, GD, and Buckley, RH. "Thymic output, T-cell diversity, and T-cell function in long-term human SCID chimeras." Blood 114, no. 7 (August 13, 2009): 1445-1453. Full Text Link to Item
  - Jones, SM, Pons, L, Roberts, JL, Scurlock, AM, Perry, TT, Kulis, M, Shreffler, WG, Steele, P, Henry, KA, Adair, M, Francis, JM, Durham, S, Vickery, BP, Zhong, X, and Burks, AW. "Clinical efficacy and immune regulation with peanut oral immunotherapy." The Journal of Allergy and Clinical Immunology 124, no. 2 (August 2009): 292-300.97. Full Text
  - Blanco, BD, Roberts, JL, Zamarreño, N, Balmelle-Devaux, N, and Hernández-Munain, C. "Flexible stereospecific interactions and composition within nucleoprotein complexes assembled on the TCRα gene enhancer." Journal of Immunology 183, no. 3 (2009): 1871-1883. Full Text
  - Palmer, K, Green, TD, Roberts, JL, Sajaroff, E, Cooney, M, Parrott, R, Chen, D-F, Reinsmoen, NL, and Buckley, RH. "Unusual clinical and immunologic manifestations of transplacentally acquired maternal T cells in severe combined immunodeficiency." J Allergy Clin Immunol 120, no. 2 (August 2007): 423-428. Full Text Link to Item
  - Roberts, JL, Lauritsen, JPH, Cooney, M, Parrott, RE, Sajaroff, EO, Win, CM, Keller, MD, Carpenter, JH, Carabana, J, Krangel, MS, Sarzotti, M, Zhong, X-P, Wiest, DL, and Buckley, RH. "T-B+NK+ severe combined immunodeficiency caused by complete deficiency of the CD3zeta subunit of the T-cell antigen receptor complex." Blood 109, no. 8 (April 15, 2007): 3198-3206. Full Text Link to Item
  - Ponda, P, Schuval, SJ, Kaplan, B, Logalbo, P, Roberts, JL, and Bonagura, VR. "Interleukin 7 receptor α-chain-mutation severe combined immunodeficiency without lymphopenia: Correction with haploidentical T-cell-depleted bone marrow transplantation." Annals of Allergy, Asthma and Immunology 97, no. 6 (2006): 755-758.
  - O'Shea, JJ, Husa, M, Li, D, Hofmann, SR, Watford, W, Roberts, JL, Buckley, RH, Changelian, P, and Candotti, F. "Jak3 and the pathogenesis of severe combined immunodeficiency." Molecular Immunology 41, no. 6-7 (July 2004): 727-737. (Review) Full Text
  - Roberts, JL, Lengi, A, Brown, SM, Chen, M, Zhou, Y-J, O'Shea, JJ, and Buckley, RH. "Janus kinase 3 (JAK3) deficiency: clinical, immunologic, and molecular analyses of 10 patients and outcomes of stem cell transplantation." Blood 103, no. 6 (March 2004): 2009-2018. Full Text
  - Zhou, YJ, Chen, M, Cusack, NA, Kimmel, LH, Magnuson, KS, Boyd, JG, Lin, W, Roberts, JL, Lengi, A, Buckley, RH, Geahlen, RL, Candotti, F, Gadina, M, Changelian, PS, and O'Shea, JJ. "Unexpected effects of FERM domain mutations on catalytic activity of Jak3: structural implication for Janus kinases." Molecular Cell 8, no. 5 (November 2001): 959-969. Full Text
  - Buckley, RH, Schiff, SE, Schiff, RI, Markert, L, Williams, LW, Roberts, JL, Myers, LA, and Ward, FE. "Hematopoietic stem-cell transplantation for the treatment of severe combined immunodeficiency." The New England Journal of Medicine 340, no. 7 (February 1999): 508-516. Full Text
  - Jabara, HH, Buckley, RH, Roberts, JL, Lefranc, G, Loiselet, J, Khalil, G, and Geha, RS. "Role of JAK3 in CD40-mediated signaling." Blood 92, no. 7 (October 1, 1998): 2435-2440. Link to Item
  - Krangel, MS, Hernandez-Munain, C, Lauzurica, P, McMurry, M, Roberts, JL, and Zhong, XP. "Developmental regulation of V(D)J recombination at the TCR alpha/delta locus." Immunol Rev 165 (October 1998): 131-147. (Review) Link to Item
  - Hernandez-Munain, C, Roberts, JL, and Krangel, MS. "Cooperation among multiple transcription factors is required for access to minimal T-cell receptor alpha-enhancer chromatin in vivo." Molecular and Cellular Biology 18, no. 6 (June 1998): 3223-3233. Full Text
  - Jabara, HH, Buckley, RH, Roberts, JL, Lefranc, G, Loiselet, J, Khalil, G, and Geha, RS. "Role of JAK3 in CD40 mediated signaling." FASEB Journal 12, no. 5 (1998): A923-.
  - Lauzurica, P, Zhong, XP, Krangel, MS, and Roberts, JL. "Regulation of T cell receptor delta gene rearrangement by CBF/PEBP2." The Journal of Experimental Medicine 185, no. 7 (April 1997): 1193-1201. Full Text
  - Buckley, RH, Schiff, RI, Schiff, SE, Markert, ML, Williams, LW, Harville, TO, Roberts, JL, and Puck, JM. "Human severe combined immunodeficiency: genetic, phenotypic, and functional diversity in one hundred eight infants." J Pediatr 130, no. 3 (March 1997): 378-387. Link to Item
  - Roberts, JL, Lauzurica, P, and Krangel, MS. "Developmental regulation of VDJ recombination by the core fragment of the T cell receptor alpha enhancer." The Journal of Experimental Medicine 185, no. 1 (January 1997): 131-140. Full Text
  - Russell, SM, Tayebi, N, Nakajima, H, Riedy, MC, Roberts, JL, Aman, MJ, Migone, TS, Noguchi, M, Markert, ML, Buckley, RH, O'Shea, JJ, and Leonard, WJ. "Mutation of Jak3 in a patient with SCID: essential role of Jak3 in lymphoid development." Science (New York, N.Y.) 270, no. 5237 (November 1995): 797-800. Full Text
  - Kearse, KP, Roberts, JL, and Singer, A. "TCR alpha-CD3 delta epsilon association is the initial step in alpha beta dimer formation in murine T cells and is limiting in immature CD4+ CD8+ thymocytes." Immunity 2, no. 4 (April 1995): 391-399. Link to Item
  - Roberts, JL, Sharrow, SO, and Singer, A. "Disruption of intrathymic CD4-Ia interactions on immature CD4+CD8+ thymocytes results in diminished TCR expression on mature CD8+ T cell progeny." Cell Immunol 161, no. 1 (March 1995): 22-27. Link to Item
  - Keanse, KP, Roberts, JL, and Singer, A. "TCRα-CD3δε{lunate} association is the initial step in αβ dimer formation in murine T cells and is limiting in immature CD4⁺CD8⁺ thymocytes." Immunity 2, no. 4 (1995): 391-399.
  - Kearse, KP, Roberts, JP, Wiest, DL, and Singer, A. "Developmental regulation of αβ T cell antigen receptor assembly in immature CD4⁺CD8⁺ thymocytes." BioEssays 17, no. 12 (1995): 1049-1054.
  - Kearse, KP, Roberts, JL, Munitz, TI, Wiest, DL, Nakayama, T, and Singer, A. "Developmental regulation of alpha beta T cell antigen receptor expression results from differential stability of nascent TCR alpha proteins within the endoplasmic reticulum of immature and mature T cells." The Embo Journal 13, no. 19 (October 1994): 4504-4514. Full Text
  - Punt, JA, Roberts, JL, Kearse, KP, and Singer, A. "Stoichiometry of the T cell antigen receptor (TCR) complex: each TCR/CD3 complex contains one TCR alpha, one TCR beta, and two CD3 epsilon chains." J Exp Med 180, no. 2 (August 1, 1994): 587-593. Link to Item
  - Buckley, RH, Schiff, SE, Schiff, RI, Roberts, JL, Markert, ML, Peters, W, Williams, LW, and Ward, FE. "Haploidentical bone marrow stem cell transplantation in human severe combined immunodeficiency." Semin Hematol 30, no. 4 Suppl 4 (October 1993): 92-101. (Review) Link to Item
  - Abe, R, Kozak, CA, Foo-Phillips, M, Roberts, J, and Principato, MAC. "Involvement of Multiple Factors in the Clonal Deletion of Self-Reactive T Cells." Cellular Immunology 151, no. 2 (1993): 425-436. Full Text
  - Buckley, RH, Schiff, SE, Schiff, RI, Roberts, JL, Markert, ML, Peters, W, Williams, LW, Ward, FE, Ballow, , Boxer, , Strober, , McGhee, , Schwartz, , Nelson, , Puck, , Ochs, , Parkman, , and Deeg, . "Haploidentical bone marrow stem cell transplantation in human severe combined immunodeficiency." Seminars in Hematology 30, no. 4 SUPPL. 4 (1993): 92-104.
  - Roberts, JL, Abe, R, Shores, EW, and Singer, A. "Expression of Mls determinants in mice exhibiting the severe combined immunodeficiency (scid) mutation or X-linked immunodeficiency (xid) defect." J Immunol 149, no. 5 (September 1, 1992): 1577-1582. Link to Item
  - Roberts, JL, Sharrow, SO, and Singer, A. "Clonal deletion and clonal anergy in the thymus induced by cellular elements with different radiation sensitivities." Journal of Experimental Medicine 171, no. 3 (1990): 935-940.
  - Roberts, JL, Volkman, DJ, and Buckley, RH. "Modified MHC restriction of donor-origin T cells in humans with severe combined immunodeficiency transplanted with haploidentical bone marrow stem cells." J Immunol 143, no. 5 (September 1, 1989): 1575-1579. Link to Item
Teaching & Mentoring
Recent Courses
- PEDS 433C: Allergy and Clinical Immunology 2018

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Education, Training, & Certifications

Medical Licensure

Duke Appointment History

Selected Grants

Selected Publications

Conference Papers

Journal Articles

Recent Courses