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Commensal microbiota stimulate systemic neutrophil migration through induction of serum amyloid A.

Publication ,  Journal Article
Kanther, M; Tomkovich, S; Xiaolun, S; Grosser, MR; Koo, J; Flynn, EJ; Jobin, C; Rawls, JF
Published in: Cell Microbiol
July 2014

Neutrophils serve critical roles in inflammatory responses to infection and injury, and mechanisms governing their activity represent attractive targets for controlling inflammation. The commensal microbiota is known to regulate the activity of neutrophils and other leucocytes in the intestine, but the systemic impact of the microbiota on neutrophils remains unknown. Here we utilized in vivo imaging in gnotobiotic zebrafish to reveal diverse effects of microbiota colonization on systemic neutrophil development and function. The presence of a microbiota resulted in increased neutrophil number and myeloperoxidase expression, and altered neutrophil localization and migratory behaviours. These effects of the microbiota on neutrophil homeostasis were accompanied by an increased recruitment of neutrophils to injury. Genetic analysis identified the microbiota-induced acute phase protein serum amyloid A (Saa) as a host factor mediating microbial stimulation of tissue-specific neutrophil migratory behaviours. In vitro studies revealed that zebrafish cells respond to Saa exposure by activating NF-κB, and that Saa-dependent neutrophil migration requires NF-κB-dependent gene expression. These results implicate the commensal microbiota as an important environmental factor regulating diverse aspects of systemic neutrophil development and function, and reveal a critical role for a Saa-NF-κB signalling axis in mediating neutrophil migratory responses.

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Published In

Cell Microbiol

DOI

EISSN

1462-5822

Publication Date

July 2014

Volume

16

Issue

7

Start / End Page

1053 / 1067

Location

India

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Wound Healing
  • Transcriptional Activation
  • Signal Transduction
  • Serum Amyloid A Protein
  • Organ Specificity
  • Neutrophils
  • NF-kappa B
  • Microbiota
 

Citation

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Chicago
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Kanther, M., Tomkovich, S., Xiaolun, S., Grosser, M. R., Koo, J., Flynn, E. J., … Rawls, J. F. (2014). Commensal microbiota stimulate systemic neutrophil migration through induction of serum amyloid A. Cell Microbiol, 16(7), 1053–1067. https://doi.org/10.1111/cmi.12257
Kanther, Michelle, Sarah Tomkovich, Sun Xiaolun, Melinda R. Grosser, Jaseol Koo, Edward J. Flynn, Christian Jobin, and John F. Rawls. “Commensal microbiota stimulate systemic neutrophil migration through induction of serum amyloid A.Cell Microbiol 16, no. 7 (July 2014): 1053–67. https://doi.org/10.1111/cmi.12257.
Kanther M, Tomkovich S, Xiaolun S, Grosser MR, Koo J, Flynn EJ, et al. Commensal microbiota stimulate systemic neutrophil migration through induction of serum amyloid A. Cell Microbiol. 2014 Jul;16(7):1053–67.
Kanther, Michelle, et al. “Commensal microbiota stimulate systemic neutrophil migration through induction of serum amyloid A.Cell Microbiol, vol. 16, no. 7, July 2014, pp. 1053–67. Pubmed, doi:10.1111/cmi.12257.
Kanther M, Tomkovich S, Xiaolun S, Grosser MR, Koo J, Flynn EJ, Jobin C, Rawls JF. Commensal microbiota stimulate systemic neutrophil migration through induction of serum amyloid A. Cell Microbiol. 2014 Jul;16(7):1053–1067.
Journal cover image

Published In

Cell Microbiol

DOI

EISSN

1462-5822

Publication Date

July 2014

Volume

16

Issue

7

Start / End Page

1053 / 1067

Location

India

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Wound Healing
  • Transcriptional Activation
  • Signal Transduction
  • Serum Amyloid A Protein
  • Organ Specificity
  • Neutrophils
  • NF-kappa B
  • Microbiota