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Direct analysis of traditional Chinese medicines using Surface-Enhanced Raman Scattering (SERS).

Publication ,  Journal Article
Zhao, J; Liu, Y; Fales, AM; Register, J; Yuan, H; Vo-Dinh, T
Published in: Drug testing and analysis
October 2014

Surface-Enhanced Raman Scattering (SERS) spectrometry provides an excellent tool to characterize chemical constituents in Traditional Chinese Medicines (TCMs) without requiring separation and extraction procedures. This study involved the use of SERS to analyze two TCMs, namely Coptis chinensis and Phellodendron amurense, and their main active constituent, berberine. Using silver nanospheres as SERS-active probes, the decoctions of two raw TCMs and their counterfeits were analyzed. Density functional theory (DFT) was used to calculate the expected Raman spectrum of berberine, and liquid chromatography- mass spectrometry (LC-MS) was used as a comparative technique to quantify the amount of berberine in the samples. The results of the SERS measurements were consistent with the results of DFT calculations and LCMS analyses. To our knowledge, this is the first time that the potential of SERS was demonstrated as a sensitive, rapid, and non-destructive method to qualitatively and quantitatively analyze the active constituents in raw TCM products.

Duke Scholars

Published In

Drug testing and analysis

DOI

EISSN

1942-7611

ISSN

1942-7603

Publication Date

October 2014

Volume

6

Issue

10

Start / End Page

1063 / 1068

Related Subject Headings

  • Spectrum Analysis, Raman
  • Silver
  • Sensitivity and Specificity
  • Phellodendron
  • Metal Nanoparticles
  • Medicine, Chinese Traditional
  • Mass Spectrometry
  • Drugs, Chinese Herbal
  • Coptis
  • Chromatography, Liquid
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zhao, J., Liu, Y., Fales, A. M., Register, J., Yuan, H., & Vo-Dinh, T. (2014). Direct analysis of traditional Chinese medicines using Surface-Enhanced Raman Scattering (SERS). Drug Testing and Analysis, 6(10), 1063–1068. https://doi.org/10.1002/dta.1612
Zhao, Jing, Yang Liu, Andrew M. Fales, Janna Register, Hsiangkuo Yuan, and Tuan Vo-Dinh. “Direct analysis of traditional Chinese medicines using Surface-Enhanced Raman Scattering (SERS).Drug Testing and Analysis 6, no. 10 (October 2014): 1063–68. https://doi.org/10.1002/dta.1612.
Zhao J, Liu Y, Fales AM, Register J, Yuan H, Vo-Dinh T. Direct analysis of traditional Chinese medicines using Surface-Enhanced Raman Scattering (SERS). Drug testing and analysis. 2014 Oct;6(10):1063–8.
Zhao, Jing, et al. “Direct analysis of traditional Chinese medicines using Surface-Enhanced Raman Scattering (SERS).Drug Testing and Analysis, vol. 6, no. 10, Oct. 2014, pp. 1063–68. Epmc, doi:10.1002/dta.1612.
Zhao J, Liu Y, Fales AM, Register J, Yuan H, Vo-Dinh T. Direct analysis of traditional Chinese medicines using Surface-Enhanced Raman Scattering (SERS). Drug testing and analysis. 2014 Oct;6(10):1063–1068.
Journal cover image

Published In

Drug testing and analysis

DOI

EISSN

1942-7611

ISSN

1942-7603

Publication Date

October 2014

Volume

6

Issue

10

Start / End Page

1063 / 1068

Related Subject Headings

  • Spectrum Analysis, Raman
  • Silver
  • Sensitivity and Specificity
  • Phellodendron
  • Metal Nanoparticles
  • Medicine, Chinese Traditional
  • Mass Spectrometry
  • Drugs, Chinese Herbal
  • Coptis
  • Chromatography, Liquid