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CERKL interacts with mitochondrial TRX2 and protects retinal cells from oxidative stress-induced apoptosis.

Publication ,  Journal Article
Li, C; Wang, L; Zhang, J; Huang, M; Wong, F; Liu, X; Liu, F; Cui, X; Yang, G; Chen, J; Liu, Y; Wang, J; Liao, S; Gao, M; Hu, X; Shu, X ...
Published in: Biochim Biophys Acta
July 2014

Mutations in the ceramide kinase-like gene (CERKL) are associated with severe retinal degeneration. However, the exact function of the encoded protein (CERKL) remains unknown. Here we show that CERKL interacts with mitochondrial thioredoxin 2 (TRX2) and maintains TRX2 in the reduced redox state. Overexpression of CERKL protects cells from apoptosis under oxidative stress, whereas suppressing CERKL renders cells more sensitive to oxidative stress. In zebrafish, CERKL protein prominently locates in the outer segment and inner segment of the photoreceptor of the retina. Knockdown of CERKL in the zebrafish leads to an increase of retinal cell death, including cone and rod photoreceptor degeneration. Signs of oxidative damage to macromolecules were also detected in CERKL deficient zebrafish retina. Our results show that CERKL interacts with TRX2 and plays a novel key role in the regulation of the TRX2 antioxidant pathway and, for the first time, provides an explanation of how mutations in CERKL may lead to retinal cell death.

Duke Scholars

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

July 2014

Volume

1842

Issue

7

Start / End Page

1121 / 1129

Location

Netherlands

Related Subject Headings

  • Zebrafish
  • Thioredoxins
  • Retina
  • Phosphotransferases (Alcohol Group Acceptor)
  • Oxidative Stress
  • Oxidation-Reduction
  • NIH 3T3 Cells
  • Mitochondrial Proteins
  • Mitochondria
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Li, C., Wang, L., Zhang, J., Huang, M., Wong, F., Liu, X., … Liu, M. (2014). CERKL interacts with mitochondrial TRX2 and protects retinal cells from oxidative stress-induced apoptosis. Biochim Biophys Acta, 1842(7), 1121–1129. https://doi.org/10.1016/j.bbadis.2014.04.009
Li, Chang, Lei Wang, Jing Zhang, Mi Huang, Fulton Wong, Xuexue Liu, Fei Liu, et al. “CERKL interacts with mitochondrial TRX2 and protects retinal cells from oxidative stress-induced apoptosis.Biochim Biophys Acta 1842, no. 7 (July 2014): 1121–29. https://doi.org/10.1016/j.bbadis.2014.04.009.
Li C, Wang L, Zhang J, Huang M, Wong F, Liu X, et al. CERKL interacts with mitochondrial TRX2 and protects retinal cells from oxidative stress-induced apoptosis. Biochim Biophys Acta. 2014 Jul;1842(7):1121–9.
Li, Chang, et al. “CERKL interacts with mitochondrial TRX2 and protects retinal cells from oxidative stress-induced apoptosis.Biochim Biophys Acta, vol. 1842, no. 7, July 2014, pp. 1121–29. Pubmed, doi:10.1016/j.bbadis.2014.04.009.
Li C, Wang L, Zhang J, Huang M, Wong F, Liu X, Liu F, Cui X, Yang G, Chen J, Liu Y, Wang J, Liao S, Gao M, Hu X, Shu X, Wang Q, Yin Z, Tang Z, Liu M. CERKL interacts with mitochondrial TRX2 and protects retinal cells from oxidative stress-induced apoptosis. Biochim Biophys Acta. 2014 Jul;1842(7):1121–1129.

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

July 2014

Volume

1842

Issue

7

Start / End Page

1121 / 1129

Location

Netherlands

Related Subject Headings

  • Zebrafish
  • Thioredoxins
  • Retina
  • Phosphotransferases (Alcohol Group Acceptor)
  • Oxidative Stress
  • Oxidation-Reduction
  • NIH 3T3 Cells
  • Mitochondrial Proteins
  • Mitochondria
  • Mice