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FRAP analysis reveals stabilization of adhesion structures in the epidermis compared to cultured keratinocytes.

Publication ,  Journal Article
Foote, HP; Sumigray, KD; Lechler, T
Published in: PLoS One
2013

Proper development and tissue maintenance requires cell-cell adhesion structures, which serve diverse and crucial roles in tissue morphogenesis. Epithelial tissues have three main types of cell-cell junctions: tight junctions, which play a major role in barrier formation, and adherens junctions and desmosomes, which provide mechanical stability and organize the underlying cytoskeleton. Our current understanding of adhesion function is hindered by a lack of tools and methods to image junctions in mammals. To better understand the dynamics of adhesion in tissues we have created a knock-in ZO-1-GFP mouse and a BAC-transgenic mouse expressing desmoplakin I-GFP. We performed fluorescence recovery after photobleaching (FRAP) experiments to quantify the turnover rates of the tight junction protein ZO-1, the adherens junction protein E-cadherin, and the desmosomal protein desmoplakin in the epidermis. Proteins at each type of junction are remarkably stable in the epidermis, in contrast to the high observed mobility of E-cadherin and ZO-1 at adherens junctions and tight junctions, respectively, in cultured cells. Our data demonstrate that there are additional mechanisms for stabilizing junctions in tissues that are not modeled by cell culture.

Duke Scholars

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2013

Volume

8

Issue

8

Start / End Page

e71491

Location

United States

Related Subject Headings

  • Zonula Occludens-1 Protein
  • Protein Isoforms
  • Mice
  • Keratinocytes
  • Green Fluorescent Proteins
  • General Science & Technology
  • Gene Knock-In Techniques
  • Fluorescence Recovery After Photobleaching
  • Epidermis
  • Epidermal Cells
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Foote, H. P., Sumigray, K. D., & Lechler, T. (2013). FRAP analysis reveals stabilization of adhesion structures in the epidermis compared to cultured keratinocytes. PLoS One, 8(8), e71491. https://doi.org/10.1371/journal.pone.0071491
Foote, Henry P., Kaelyn D. Sumigray, and Terry Lechler. “FRAP analysis reveals stabilization of adhesion structures in the epidermis compared to cultured keratinocytes.PLoS One 8, no. 8 (2013): e71491. https://doi.org/10.1371/journal.pone.0071491.
Foote, Henry P., et al. “FRAP analysis reveals stabilization of adhesion structures in the epidermis compared to cultured keratinocytes.PLoS One, vol. 8, no. 8, 2013, p. e71491. Pubmed, doi:10.1371/journal.pone.0071491.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2013

Volume

8

Issue

8

Start / End Page

e71491

Location

United States

Related Subject Headings

  • Zonula Occludens-1 Protein
  • Protein Isoforms
  • Mice
  • Keratinocytes
  • Green Fluorescent Proteins
  • General Science & Technology
  • Gene Knock-In Techniques
  • Fluorescence Recovery After Photobleaching
  • Epidermis
  • Epidermal Cells