Analyzing transcription dynamics during the budding yeast cell cycle.
Publication
, Journal Article
Leman, AR; Bristow, SL; Haase, SB
Published in: Methods in molecular biology (Clifton, N.J.)
January 2014
Assaying global cell cycle-regulated transcription in budding yeast involves extracting RNA from a synchronous population and proper normalization of detected transcript levels. Here, we describe synchronization of Saccharomyces cerevisiae cell populations by centrifugal elutriation, followed by the isolation of RNA for microarray analysis. Further, we outline the computational methods required to directly compare RNA abundance from individual time points within an experiment and to compare independent experiments. Together, these methods describe the complete workflow necessary to observe RNA abundance during the cell cycle.
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Published In
Methods in molecular biology (Clifton, N.J.)
DOI
EISSN
1940-6029
ISSN
1064-3745
Publication Date
January 2014
Volume
1170
Start / End Page
295 / 312
Related Subject Headings
- Transcriptional Activation
- Saccharomycetales
- RNA, Fungal
- Oligonucleotide Array Sequence Analysis
- Gene Expression Regulation, Fungal
- Electrophoresis
- Developmental Biology
- Centrifugation
- Cell Separation
- Cell Cycle
Citation
APA
Chicago
ICMJE
MLA
NLM
Leman, A. R., Bristow, S. L., & Haase, S. B. (2014). Analyzing transcription dynamics during the budding yeast cell cycle. Methods in Molecular Biology (Clifton, N.J.), 1170, 295–312. https://doi.org/10.1007/978-1-4939-0888-2_14
Leman, Adam R., Sara L. Bristow, and Steven B. Haase. “Analyzing transcription dynamics during the budding yeast cell cycle.” Methods in Molecular Biology (Clifton, N.J.) 1170 (January 2014): 295–312. https://doi.org/10.1007/978-1-4939-0888-2_14.
Leman AR, Bristow SL, Haase SB. Analyzing transcription dynamics during the budding yeast cell cycle. Methods in molecular biology (Clifton, NJ). 2014 Jan;1170:295–312.
Leman, Adam R., et al. “Analyzing transcription dynamics during the budding yeast cell cycle.” Methods in Molecular Biology (Clifton, N.J.), vol. 1170, Jan. 2014, pp. 295–312. Epmc, doi:10.1007/978-1-4939-0888-2_14.
Leman AR, Bristow SL, Haase SB. Analyzing transcription dynamics during the budding yeast cell cycle. Methods in molecular biology (Clifton, NJ). 2014 Jan;1170:295–312.
Published In
Methods in molecular biology (Clifton, N.J.)
DOI
EISSN
1940-6029
ISSN
1064-3745
Publication Date
January 2014
Volume
1170
Start / End Page
295 / 312
Related Subject Headings
- Transcriptional Activation
- Saccharomycetales
- RNA, Fungal
- Oligonucleotide Array Sequence Analysis
- Gene Expression Regulation, Fungal
- Electrophoresis
- Developmental Biology
- Centrifugation
- Cell Separation
- Cell Cycle