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Proteome and calcium-related gene expression in Pinus massoniana needles in response to acid rain under different calcium levels

Publication ,  Journal Article
Hu, WJ; Chen, J; Liu, TW; Wu, Q; Wang, WH; Liu, X; Shen, ZJ; Simon, M; Wu, FH; Pei, ZM; Zheng, HL
Published in: Plant and Soil
January 1, 2014

Aims: Calcium (Ca) is a crucial regulator of plant growth, development and stress responses. Acid rain (AR), a serious environmental issue worldwide, leaches away Ca from surrounding soils and affects vegetation. The objective of this study was to investigate how different Ca levels affect protein and RNA expression of calcium-related genes under AR stress. Methods: Pinus massoniana seedlings grown at high, medium, and low Ca levels under AR stress were used for proteomics analysis. Furthermore, relative mRNA levels of eight Ca-related genes and their interaction network were analyzed by quantitative real-time PCR (qRT-PCR) and bioinformatic analysis. Results: Proteomic studies identified 95 differentially expressed proteins which were classified into eight groups, including metabolic processes, photosynthesis, and cell rescue and defense, etc. Moreover, qRT-PCR results indicated that low Ca significantly increased the expression level of the investigated Ca-related genes, which can be reversed by high Ca under AR stress. Conclusions: Defense system, gene transcription and translation, photosynthetic machinery, and substance and energy metabolism may play important roles in the responses of P. massoniana to AR stress. Low Ca increases the abundance of cell rescue and defense-related proteins under AR. High Ca can reduce AR damage by increasing the abundance of proteins involved in the Calvin cycle, glycolysis, TCA cycle, and nitrogen metabolism. Our results indicated a possible mechanism involving Ca in AR tolerance in plants. © 2014 Springer International Publishing Switzerland.

Duke Scholars

Published In

Plant and Soil

DOI

ISSN

0032-079X

Publication Date

January 1, 2014

Volume

380

Issue

1

Start / End Page

285 / 303

Related Subject Headings

  • Agronomy & Agriculture
  • 07 Agricultural and Veterinary Sciences
  • 06 Biological Sciences
  • 05 Environmental Sciences
 

Citation

APA
Chicago
ICMJE
MLA
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Hu, W. J., Chen, J., Liu, T. W., Wu, Q., Wang, W. H., Liu, X., … Zheng, H. L. (2014). Proteome and calcium-related gene expression in Pinus massoniana needles in response to acid rain under different calcium levels. Plant and Soil, 380(1), 285–303. https://doi.org/10.1007/s11104-014-2086-9
Hu, W. J., J. Chen, T. W. Liu, Q. Wu, W. H. Wang, X. Liu, Z. J. Shen, et al. “Proteome and calcium-related gene expression in Pinus massoniana needles in response to acid rain under different calcium levels.” Plant and Soil 380, no. 1 (January 1, 2014): 285–303. https://doi.org/10.1007/s11104-014-2086-9.
Hu WJ, Chen J, Liu TW, Wu Q, Wang WH, Liu X, et al. Proteome and calcium-related gene expression in Pinus massoniana needles in response to acid rain under different calcium levels. Plant and Soil. 2014 Jan 1;380(1):285–303.
Hu, W. J., et al. “Proteome and calcium-related gene expression in Pinus massoniana needles in response to acid rain under different calcium levels.” Plant and Soil, vol. 380, no. 1, Jan. 2014, pp. 285–303. Scopus, doi:10.1007/s11104-014-2086-9.
Hu WJ, Chen J, Liu TW, Wu Q, Wang WH, Liu X, Shen ZJ, Simon M, Wu FH, Pei ZM, Zheng HL. Proteome and calcium-related gene expression in Pinus massoniana needles in response to acid rain under different calcium levels. Plant and Soil. 2014 Jan 1;380(1):285–303.
Journal cover image

Published In

Plant and Soil

DOI

ISSN

0032-079X

Publication Date

January 1, 2014

Volume

380

Issue

1

Start / End Page

285 / 303

Related Subject Headings

  • Agronomy & Agriculture
  • 07 Agricultural and Veterinary Sciences
  • 06 Biological Sciences
  • 05 Environmental Sciences