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A high-resolution cardiovascular magnetic resonance diffusion tensor map from ex-vivo C57BL/6 murine hearts.

Publication ,  Journal Article
Angeli, S; Befera, N; Peyrat, J-M; Calabrese, E; Johnson, GA; Constantinides, C
Published in: J Cardiovasc Magn Reson
October 16, 2014

BACKGROUND: The complex cardiac fiber structural organization and spatial arrangement of cardiomyocytes in laminar sheetlets contributes greatly to cardiac functional and contractile ejection patterns. This study presents the first comprehensive, ultra-high resolution, fully quantitative statistical tensor map of the fixed murine heart at isotropic resolution of 43 μm using diffusion tensor (DT) cardiovascular magnetic resonance (CMR). METHODS: Imaging was completed in approximately 12 hours using a six-directional encoding scheme, in five ex vivo healthy C57BL/6 mouse hearts. The tensor map constructed from this data provides an average description of the murine fiber architecture visualized with fiber tractography, and its population variability, using the latest advances in image tensor analysis and statistics. RESULTS: Results show that non-normalized cardiac tensor maps are associated with mean fractional anisotropy of 0.25 ± 0.07 and mean diffusivity of 8.9 ± 1.6 × 10⁻⁴mm²/s. Moreover, average mid-ventricular helical angle distributions ranged between -41 ± 3° and +52 ± 5° and were highly correlated with transmural depth, in agreement with prior published results in humans and canines. Calculated variabilities of local myocyte orientations were 2.0° and 1.4°. Laminar sheet orientation variability was found to be less stable at 2.6°. Despite such variations, the murine heart seems to be highly structured, particularly when compared to canines and humans. CONCLUSIONS: This tensor map has the potential to yield an accurate mean representation and identification of common or unique features of the cardiac myocyte architecture, to establish a baseline standard reference of DTI indices, and to improve detection of biomarkers, especially in pathological states or post-transgenetic modifications.

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Published In

J Cardiovasc Magn Reson

DOI

EISSN

1532-429X

Publication Date

October 16, 2014

Volume

16

Issue

1

Start / End Page

77

Location

England

Related Subject Headings

  • Predictive Value of Tests
  • Nuclear Medicine & Medical Imaging
  • Myocytes, Cardiac
  • Myocardium
  • Mice, Inbred C57BL
  • Male
  • Heart
  • Diffusion Tensor Imaging
  • Diffusion Magnetic Resonance Imaging
  • Animals
 

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Angeli, S., Befera, N., Peyrat, J.-M., Calabrese, E., Johnson, G. A., & Constantinides, C. (2014). A high-resolution cardiovascular magnetic resonance diffusion tensor map from ex-vivo C57BL/6 murine hearts. J Cardiovasc Magn Reson, 16(1), 77. https://doi.org/10.1186/s12968-014-0077-x
Angeli, Stelios, Nicholas Befera, Jean-Marc Peyrat, Evan Calabrese, George Allan Johnson, and Christakis Constantinides. “A high-resolution cardiovascular magnetic resonance diffusion tensor map from ex-vivo C57BL/6 murine hearts.J Cardiovasc Magn Reson 16, no. 1 (October 16, 2014): 77. https://doi.org/10.1186/s12968-014-0077-x.
Angeli S, Befera N, Peyrat J-M, Calabrese E, Johnson GA, Constantinides C. A high-resolution cardiovascular magnetic resonance diffusion tensor map from ex-vivo C57BL/6 murine hearts. J Cardiovasc Magn Reson. 2014 Oct 16;16(1):77.
Angeli, Stelios, et al. “A high-resolution cardiovascular magnetic resonance diffusion tensor map from ex-vivo C57BL/6 murine hearts.J Cardiovasc Magn Reson, vol. 16, no. 1, Oct. 2014, p. 77. Pubmed, doi:10.1186/s12968-014-0077-x.
Angeli S, Befera N, Peyrat J-M, Calabrese E, Johnson GA, Constantinides C. A high-resolution cardiovascular magnetic resonance diffusion tensor map from ex-vivo C57BL/6 murine hearts. J Cardiovasc Magn Reson. 2014 Oct 16;16(1):77.
Journal cover image

Published In

J Cardiovasc Magn Reson

DOI

EISSN

1532-429X

Publication Date

October 16, 2014

Volume

16

Issue

1

Start / End Page

77

Location

England

Related Subject Headings

  • Predictive Value of Tests
  • Nuclear Medicine & Medical Imaging
  • Myocytes, Cardiac
  • Myocardium
  • Mice, Inbred C57BL
  • Male
  • Heart
  • Diffusion Tensor Imaging
  • Diffusion Magnetic Resonance Imaging
  • Animals