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A regulatory hierarchy controls the dynamic transcriptional response to extreme oxidative stress in archaea.

Publication ,  Journal Article
Tonner, PD; Pittman, AMC; Gulli, JG; Sharma, K; Schmid, AK
Published in: PLoS genetics
January 2015

Networks of interacting transcription factors are central to the regulation of cellular responses to abiotic stress. Although the architecture of many such networks has been mapped, their dynamic function remains unclear. Here we address this challenge in archaea, microorganisms possessing transcription factors that resemble those of both eukaryotes and bacteria. Using genome-wide DNA binding location analysis integrated with gene expression and cell physiological data, we demonstrate that a bacterial-type transcription factor (TF), called RosR, and five TFIIB proteins, homologs of eukaryotic TFs, combinatorially regulate over 100 target genes important for the response to extremely high levels of peroxide. These genes include 20 other transcription factors and oxidative damage repair genes. RosR promoter occupancy is surprisingly dynamic, with the pattern of target gene expression during the transition from rapid growth to stress correlating strongly with the pattern of dynamic binding. We conclude that a hierarchical regulatory network orchestrated by TFs of hybrid lineage enables dynamic response and survival under extreme stress in archaea. This raises questions regarding the evolutionary trajectory of gene networks in response to stress.

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Published In

PLoS genetics

DOI

EISSN

1553-7404

ISSN

1553-7390

Publication Date

January 2015

Volume

11

Issue

1

Start / End Page

e1004912

Related Subject Headings

  • Transcription Factor TFIIB
  • Oxidative Stress
  • Nucleotide Motifs
  • Gene Regulatory Networks
  • Gene Expression Regulation, Bacterial
  • Developmental Biology
  • DNA-Binding Proteins
  • Archaea
  • 3105 Genetics
  • 0604 Genetics
 

Citation

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Tonner, P. D., Pittman, A. M. C., Gulli, J. G., Sharma, K., & Schmid, A. K. (2015). A regulatory hierarchy controls the dynamic transcriptional response to extreme oxidative stress in archaea. PLoS Genetics, 11(1), e1004912. https://doi.org/10.1371/journal.pgen.1004912
Tonner, Peter D., Adrianne M. C. Pittman, Jordan G. Gulli, Kriti Sharma, and Amy K. Schmid. “A regulatory hierarchy controls the dynamic transcriptional response to extreme oxidative stress in archaea.PLoS Genetics 11, no. 1 (January 2015): e1004912. https://doi.org/10.1371/journal.pgen.1004912.
Tonner PD, Pittman AMC, Gulli JG, Sharma K, Schmid AK. A regulatory hierarchy controls the dynamic transcriptional response to extreme oxidative stress in archaea. PLoS genetics. 2015 Jan;11(1):e1004912.
Tonner, Peter D., et al. “A regulatory hierarchy controls the dynamic transcriptional response to extreme oxidative stress in archaea.PLoS Genetics, vol. 11, no. 1, Jan. 2015, p. e1004912. Epmc, doi:10.1371/journal.pgen.1004912.
Tonner PD, Pittman AMC, Gulli JG, Sharma K, Schmid AK. A regulatory hierarchy controls the dynamic transcriptional response to extreme oxidative stress in archaea. PLoS genetics. 2015 Jan;11(1):e1004912.

Published In

PLoS genetics

DOI

EISSN

1553-7404

ISSN

1553-7390

Publication Date

January 2015

Volume

11

Issue

1

Start / End Page

e1004912

Related Subject Headings

  • Transcription Factor TFIIB
  • Oxidative Stress
  • Nucleotide Motifs
  • Gene Regulatory Networks
  • Gene Expression Regulation, Bacterial
  • Developmental Biology
  • DNA-Binding Proteins
  • Archaea
  • 3105 Genetics
  • 0604 Genetics