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Progenitor cell mobilization enhances bone healing by means of improved neovascularization and osteogenesis.

Publication ,  Journal Article
Wang, XX; Allen, RJ; Tutela, JP; Sailon, A; Allori, AC; Davidson, EH; Paek, GK; Saadeh, PB; McCarthy, JG; Warren, SM
Published in: Plast Reconstr Surg
August 2011

BACKGROUND: Although bone repair is a relatively efficient process, a significant portion of patients fail to heal their fractures. Because adequate blood supply is essential to osteogenesis, the authors hypothesize that augmenting neovascularization by increasing the number of circulating progenitor cells will improve bony healing. METHODS: Bilateral full-thickness defects were created in the parietal bones of C57 wild-type mice. Intraperitoneal AMD3100 (n = 33) or sterile saline (n = 33) was administered daily beginning on postoperative day 3 and continuing through day 18. Circulating progenitor cell number was quantified by fluorescence-activated cell sorting. Bone regeneration was assessed with micro-computed tomography. Immunofluorescent CD31 and osteocalcin staining was performed to assess for vascularity and osteoblast density. RESULTS: AMD3100 treatment increased circulating progenitor cell levels and significantly improved bone regeneration. Calvarial defects of AMD3100-treated mice demonstrated increased vascularity and osteoblast density. CONCLUSIONS: Improved bone regeneration in this model was associated with elevated circulating progenitor cell number and subsequently improved neovascularization and osteogenesis. These findings highlight the importance of circulating progenitor cells in bone healing and may provide a novel therapy for bone regeneration.

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Published In

Plast Reconstr Surg

DOI

EISSN

1529-4242

Publication Date

August 2011

Volume

128

Issue

2

Start / End Page

395 / 405

Location

United States

Related Subject Headings

  • X-Ray Microtomography
  • Surgery
  • Stem Cells
  • Receptors, CXCR4
  • Parietal Bone
  • Osteogenesis
  • Osteoblasts
  • Neovascularization, Physiologic
  • Mice, Inbred C57BL
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wang, X. X., Allen, R. J., Tutela, J. P., Sailon, A., Allori, A. C., Davidson, E. H., … Warren, S. M. (2011). Progenitor cell mobilization enhances bone healing by means of improved neovascularization and osteogenesis. Plast Reconstr Surg, 128(2), 395–405. https://doi.org/10.1097/PRS.0b013e31821e6e10
Wang, Xiao Xia, Robert J. Allen, John Paul Tutela, Alexander Sailon, Alexander C. Allori, Edward H. Davidson, Gina K. Paek, Pierre B. Saadeh, Joseph G. McCarthy, and Stephen M. Warren. “Progenitor cell mobilization enhances bone healing by means of improved neovascularization and osteogenesis.Plast Reconstr Surg 128, no. 2 (August 2011): 395–405. https://doi.org/10.1097/PRS.0b013e31821e6e10.
Wang XX, Allen RJ, Tutela JP, Sailon A, Allori AC, Davidson EH, et al. Progenitor cell mobilization enhances bone healing by means of improved neovascularization and osteogenesis. Plast Reconstr Surg. 2011 Aug;128(2):395–405.
Wang, Xiao Xia, et al. “Progenitor cell mobilization enhances bone healing by means of improved neovascularization and osteogenesis.Plast Reconstr Surg, vol. 128, no. 2, Aug. 2011, pp. 395–405. Pubmed, doi:10.1097/PRS.0b013e31821e6e10.
Wang XX, Allen RJ, Tutela JP, Sailon A, Allori AC, Davidson EH, Paek GK, Saadeh PB, McCarthy JG, Warren SM. Progenitor cell mobilization enhances bone healing by means of improved neovascularization and osteogenesis. Plast Reconstr Surg. 2011 Aug;128(2):395–405.

Published In

Plast Reconstr Surg

DOI

EISSN

1529-4242

Publication Date

August 2011

Volume

128

Issue

2

Start / End Page

395 / 405

Location

United States

Related Subject Headings

  • X-Ray Microtomography
  • Surgery
  • Stem Cells
  • Receptors, CXCR4
  • Parietal Bone
  • Osteogenesis
  • Osteoblasts
  • Neovascularization, Physiologic
  • Mice, Inbred C57BL
  • Mice