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Periosteum-guided prefabrication of vascularized bone of clinical shape and volume.

Publication ,  Journal Article
Cheng, M-H; Brey, EM; Allori, AC; Gassman, A; Chang, DW; Patrick, CW; Miller, MJ
Published in: Plast Reconstr Surg
September 2009

BACKGROUND: Large craniofacial skeletal defects require complex reconstruction. Vascularized tissue transfer is the current standard in treatment, but these operations are technically difficult and associated with donor-site morbidity. Guided flap prefabrication offers a technique for endogenously engineering vascularized composite tissues with complex three-dimensional structure. This study evaluates the relationship between implantation time and tissue structure for generating tissues of clinically relevant volume and structure. METHODS: Twenty skeletally mature domestic sheep were implanted with poly(methyl methacrylate) chambers designed to mimic the size and shape of the mental protuberance of the mandible. Each chamber was filled with morcellized bone graft and implanted with the open face apposed to the cambium layer of the rib periosteum. Chambers were harvested at 3, 6, 9, 12, and 24 weeks, and the tissue inside the chambers was analyzed for shape conformation to chamber geometry, gross tissue volume, and bone histomorphometric parameters. RESULTS: Histologically, active endochondral, direct, and appositional bone formation was observed. Calcified tissue area and new bone formation increased for each time point up to 12 weeks of implantation. The tissues formed maintained volumetric and geometrical structure consistent with the chamber up to 9 weeks after implantation. Significant decreases in total volume and agreement with chamber geometry were observed at 12 and 24 weeks. CONCLUSIONS: Periosteum-guided tissue prefabrication was found to be an effective means of engineering three-dimensional vascularized bone of clinical size and shape. The optimal duration of incubation before significant volume loss occurs is 9 weeks in this large-animal model.

Duke Scholars

Published In

Plast Reconstr Surg

DOI

EISSN

1529-4242

Publication Date

September 2009

Volume

124

Issue

3

Start / End Page

787 / 795

Location

United States

Related Subject Headings

  • Surgery
  • Sheep
  • Periosteum
  • Osteogenesis
  • Implants, Experimental
  • Guided Tissue Regeneration
  • Diffusion Chambers, Culture
  • Bone and Bones
  • Bone Transplantation
  • Biocompatible Materials
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Cheng, M.-H., Brey, E. M., Allori, A. C., Gassman, A., Chang, D. W., Patrick, C. W., & Miller, M. J. (2009). Periosteum-guided prefabrication of vascularized bone of clinical shape and volume. Plast Reconstr Surg, 124(3), 787–795. https://doi.org/10.1097/PRS.0b013e3181b17a91
Cheng, Ming-Huei, Eric M. Brey, Alexander C. Allori, Andrew Gassman, David W. Chang, Charles W. Patrick, and Michael J. Miller. “Periosteum-guided prefabrication of vascularized bone of clinical shape and volume.Plast Reconstr Surg 124, no. 3 (September 2009): 787–95. https://doi.org/10.1097/PRS.0b013e3181b17a91.
Cheng M-H, Brey EM, Allori AC, Gassman A, Chang DW, Patrick CW, et al. Periosteum-guided prefabrication of vascularized bone of clinical shape and volume. Plast Reconstr Surg. 2009 Sep;124(3):787–95.
Cheng, Ming-Huei, et al. “Periosteum-guided prefabrication of vascularized bone of clinical shape and volume.Plast Reconstr Surg, vol. 124, no. 3, Sept. 2009, pp. 787–95. Pubmed, doi:10.1097/PRS.0b013e3181b17a91.
Cheng M-H, Brey EM, Allori AC, Gassman A, Chang DW, Patrick CW, Miller MJ. Periosteum-guided prefabrication of vascularized bone of clinical shape and volume. Plast Reconstr Surg. 2009 Sep;124(3):787–795.

Published In

Plast Reconstr Surg

DOI

EISSN

1529-4242

Publication Date

September 2009

Volume

124

Issue

3

Start / End Page

787 / 795

Location

United States

Related Subject Headings

  • Surgery
  • Sheep
  • Periosteum
  • Osteogenesis
  • Implants, Experimental
  • Guided Tissue Regeneration
  • Diffusion Chambers, Culture
  • Bone and Bones
  • Bone Transplantation
  • Biocompatible Materials