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Pilot Study Evaluating a Rat Model of Radiation-induced Erectile Dysfunction Using an Image-guided Microirradiator.

Publication ,  Journal Article
Kimura, M; Zodda, AR; Mahmood, J; Das, SK; Nguyen, GB; Jackson, IL; Vujaskovic, Z
Published in: Urology
May 2015

OBJECTIVE: To establish a feasible rat model of radiation-induced erectile dysfunction after targeted prostate irradiation using an image-guided irradiation unit specially designed for small-animal radiation research. METHODS: The X-RAD 225Cx research platform was used in the present study. We first performed quality assurance testing using a rat cadaver. After confirming dosimetry, 24 age-matched, young, adult, male rats were assigned to sham radiation or radiation to the prostate with doses of 15, 20, or 25 Gy. To confirm appropriate prostate irradiation, physiological erectile function was evaluated using intracavernous pressure (ICP) measurements with cavernous nerve electrical stimulation at 9 weeks after radiotherapy. Each animal was weighed at the time of ICP measurement. In addition, we investigated the cyclic guanosine monophosphate level in the penile cavernosa using a commercial enzyme-linked immunosorbent assay kit. RESULTS: Quality assurance results confirmed the accuracy of the irradiation technique. Dose-dependent decreases in ICP in irradiated rats were observed without major toxicity. No difference in body weight was noted among the experimental groups. Cyclic guanosine monophosphate levels were significantly decreased in the group that received 25 Gy compared with the age-matched sham-irradiated group. CONCLUSION: High-precision imaging and targeting capabilities provided by the micro-IGRT platform enable us to develop a reproducible animal model of radiation-induced erectile dysfunction in prostate cancer research.

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Published In

Urology

DOI

EISSN

1527-9995

Publication Date

May 2015

Volume

85

Issue

5

Start / End Page

1214.e1 / 1214.e6

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Rats, Sprague-Dawley
  • Rats
  • Radiation Injuries
  • Radiation Dosage
  • Prostate
  • Pilot Projects
  • Penis
  • Male
  • Erectile Dysfunction
 

Citation

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Kimura, M., Zodda, A. R., Mahmood, J., Das, S. K., Nguyen, G. B., Jackson, I. L., & Vujaskovic, Z. (2015). Pilot Study Evaluating a Rat Model of Radiation-induced Erectile Dysfunction Using an Image-guided Microirradiator. Urology, 85(5), 1214.e1-1214.e6. https://doi.org/10.1016/j.urology.2014.12.020
Kimura, Masaki, Andrew R. Zodda, Javed Mahmood, Shiva K. Das, Giao B. Nguyen, Isabel L. Jackson, and Zeljko Vujaskovic. “Pilot Study Evaluating a Rat Model of Radiation-induced Erectile Dysfunction Using an Image-guided Microirradiator.Urology 85, no. 5 (May 2015): 1214.e1-1214.e6. https://doi.org/10.1016/j.urology.2014.12.020.
Kimura M, Zodda AR, Mahmood J, Das SK, Nguyen GB, Jackson IL, et al. Pilot Study Evaluating a Rat Model of Radiation-induced Erectile Dysfunction Using an Image-guided Microirradiator. Urology. 2015 May;85(5):1214.e1-1214.e6.
Kimura, Masaki, et al. “Pilot Study Evaluating a Rat Model of Radiation-induced Erectile Dysfunction Using an Image-guided Microirradiator.Urology, vol. 85, no. 5, May 2015, pp. 1214.e1-1214.e6. Pubmed, doi:10.1016/j.urology.2014.12.020.
Kimura M, Zodda AR, Mahmood J, Das SK, Nguyen GB, Jackson IL, Vujaskovic Z. Pilot Study Evaluating a Rat Model of Radiation-induced Erectile Dysfunction Using an Image-guided Microirradiator. Urology. 2015 May;85(5):1214.e1-1214.e6.
Journal cover image

Published In

Urology

DOI

EISSN

1527-9995

Publication Date

May 2015

Volume

85

Issue

5

Start / End Page

1214.e1 / 1214.e6

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Rats, Sprague-Dawley
  • Rats
  • Radiation Injuries
  • Radiation Dosage
  • Prostate
  • Pilot Projects
  • Penis
  • Male
  • Erectile Dysfunction