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Reduction of invasive bacteria in ethanol fermentations using bacteriophages.

Publication ,  Journal Article
Worley-Morse, TO; Deshusses, MA; Gunsch, CK
Published in: Biotechnology and bioengineering
August 2015

Invasive Lactobacillus bacteria inhibit ethanol fermentations and reduce final product yields. Due to the emergence of antibiotic resistant strains of Lactobacillus spp., alternative disinfection strategies are needed for ethanol fermentations. The feasibility of using the bacteriophage (phage) 8014-B2 to control Lactobacillus plantarum in ethanol fermentations by Saccharomyces cerevisiae was investigated. In 48 h media-based shake flask fermentations, phages achieved greater than 3-log inactivation of L. plantarum, protected final ethanol yields, and maintained yeast viability. The phage-based bacterial disinfection rates depended on both the initial phage and bacterial concentrations. Furthermore, a simple set of kinetic equations was used to model the yeast, bacteria, phage, reducing sugars, and ethanol concentrations over the course of 48 h, and the various kinetic parameters were determined. Taken together, these results demonstrate the applicability of phages to reduce L. plantarum contamination and to protect final product yields in media-based fermentations.

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Published In

Biotechnology and bioengineering

DOI

EISSN

1097-0290

ISSN

0006-3592

Publication Date

August 2015

Volume

112

Issue

8

Start / End Page

1544 / 1553

Related Subject Headings

  • Saccharomyces cerevisiae
  • Lactobacillus
  • Fermentation
  • Ethanol
  • Disinfection
  • Biotechnology
  • Bioreactors
  • Bacteriophages
  • Bacteriolysis
 

Citation

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Worley-Morse, T. O., Deshusses, M. A., & Gunsch, C. K. (2015). Reduction of invasive bacteria in ethanol fermentations using bacteriophages. Biotechnology and Bioengineering, 112(8), 1544–1553. https://doi.org/10.1002/bit.25586
Worley-Morse, Thomas O., Marc A. Deshusses, and Claudia K. Gunsch. “Reduction of invasive bacteria in ethanol fermentations using bacteriophages.Biotechnology and Bioengineering 112, no. 8 (August 2015): 1544–53. https://doi.org/10.1002/bit.25586.
Worley-Morse TO, Deshusses MA, Gunsch CK. Reduction of invasive bacteria in ethanol fermentations using bacteriophages. Biotechnology and bioengineering. 2015 Aug;112(8):1544–53.
Worley-Morse, Thomas O., et al. “Reduction of invasive bacteria in ethanol fermentations using bacteriophages.Biotechnology and Bioengineering, vol. 112, no. 8, Aug. 2015, pp. 1544–53. Epmc, doi:10.1002/bit.25586.
Worley-Morse TO, Deshusses MA, Gunsch CK. Reduction of invasive bacteria in ethanol fermentations using bacteriophages. Biotechnology and bioengineering. 2015 Aug;112(8):1544–1553.
Journal cover image

Published In

Biotechnology and bioengineering

DOI

EISSN

1097-0290

ISSN

0006-3592

Publication Date

August 2015

Volume

112

Issue

8

Start / End Page

1544 / 1553

Related Subject Headings

  • Saccharomyces cerevisiae
  • Lactobacillus
  • Fermentation
  • Ethanol
  • Disinfection
  • Biotechnology
  • Bioreactors
  • Bacteriophages
  • Bacteriolysis