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Abundance of infiltrating CD163+ cells in the retina of postmortem eyes with dry and neovascular age-related macular degeneration.

Publication ,  Journal Article
Lad, EM; Cousins, SW; Van Arnam, JS; Proia, AD
Published in: Graefes Arch Clin Exp Ophthalmol
November 2015

PURPOSE: Prior research in animal models has suggested that retinal macrophages play an important role in age-related macular degeneration (AMD), but studies have insufficiently characterized the distribution of retinal macrophages in various stages of human AMD. METHODS: In this case series, we analyzed H&E, periodic acid-Schiff, and CD163 and CD68 immunostained slides from 56 formaldehyde-fixed, paraffin-embedded autopsy eyes of patients over age 75: 11 age-matched, normal eyes, and 45 AMD eyes. RESULTS: Qualitative analysis of the macula and retinal periphery revealed that all eyes contained a significant number of CD163+ cells but a negligible number of CD68+ cells. In normal eyes and eyes with thin or infrequent basal laminar deposits, CD163+ cells were restricted to the inner retina. In contrast, in AMD eyes with thick basal deposits, choroidal neovascular membranes, and geographic atrophy, qualitatively there was a marked increase in the number and size of the CD163+ cells in the outer retina, sub-retinal, and sub-retinal pigment epithelium space in the macula. CONCLUSIONS: The changes in number and localization of retinal CD163+ cells in eyes with intermediate-severe AMD support a key role for macrophages in the pathogenesis and progression of the disease. A larger, quantitative study evaluating the distribution of macrophage subpopulations in postmortem AMD eyes is warranted.

Duke Scholars

Published In

Graefes Arch Clin Exp Ophthalmol

DOI

EISSN

1435-702X

Publication Date

November 2015

Volume

253

Issue

11

Start / End Page

1941 / 1945

Location

Germany

Related Subject Headings

  • Wet Macular Degeneration
  • Retina
  • Receptors, Scavenger
  • Receptors, Cell Surface
  • Ophthalmology & Optometry
  • Male
  • Macrophages
  • Humans
  • Geographic Atrophy
  • Female
 

Citation

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Lad, E. M., Cousins, S. W., Van Arnam, J. S., & Proia, A. D. (2015). Abundance of infiltrating CD163+ cells in the retina of postmortem eyes with dry and neovascular age-related macular degeneration. Graefes Arch Clin Exp Ophthalmol, 253(11), 1941–1945. https://doi.org/10.1007/s00417-015-3094-z
Lad, Eleonora M., Scott W. Cousins, John S. Van Arnam, and Alan D. Proia. “Abundance of infiltrating CD163+ cells in the retina of postmortem eyes with dry and neovascular age-related macular degeneration.Graefes Arch Clin Exp Ophthalmol 253, no. 11 (November 2015): 1941–45. https://doi.org/10.1007/s00417-015-3094-z.
Lad EM, Cousins SW, Van Arnam JS, Proia AD. Abundance of infiltrating CD163+ cells in the retina of postmortem eyes with dry and neovascular age-related macular degeneration. Graefes Arch Clin Exp Ophthalmol. 2015 Nov;253(11):1941–5.
Lad, Eleonora M., et al. “Abundance of infiltrating CD163+ cells in the retina of postmortem eyes with dry and neovascular age-related macular degeneration.Graefes Arch Clin Exp Ophthalmol, vol. 253, no. 11, Nov. 2015, pp. 1941–45. Pubmed, doi:10.1007/s00417-015-3094-z.
Lad EM, Cousins SW, Van Arnam JS, Proia AD. Abundance of infiltrating CD163+ cells in the retina of postmortem eyes with dry and neovascular age-related macular degeneration. Graefes Arch Clin Exp Ophthalmol. 2015 Nov;253(11):1941–1945.
Journal cover image

Published In

Graefes Arch Clin Exp Ophthalmol

DOI

EISSN

1435-702X

Publication Date

November 2015

Volume

253

Issue

11

Start / End Page

1941 / 1945

Location

Germany

Related Subject Headings

  • Wet Macular Degeneration
  • Retina
  • Receptors, Scavenger
  • Receptors, Cell Surface
  • Ophthalmology & Optometry
  • Male
  • Macrophages
  • Humans
  • Geographic Atrophy
  • Female