Analyzing transcription dynamics during the budding yeast cell cycle
Publication
, Journal Article
Leman, AR; Bristow, SL; Haase, SB
Published in: Methods in Molecular Biology
January 1, 2014
Assaying global cell cycle-regulated transcription in budding yeast involves extracting RNA from a synchronous population and proper normalization of detected transcript levels. Here, we describe synchronization of Saccharomyces cerevisiae cell populations by centrifugal elutriation, followed by the isolation of RNA for microarray analysis. Further, we outline the computational methods required to directly compare RNA abundance from individual time points within an experiment and to compare independent experiments. Together, these methods describe the complete workflow necessary to observe RNA abundance during the cell cycle. © Springer Science+Business Media New York 2014.
Duke Scholars
Published In
Methods in Molecular Biology
DOI
ISSN
1064-3745
Publication Date
January 1, 2014
Volume
1170
Start / End Page
295 / 312
Related Subject Headings
- Developmental Biology
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Leman, A. R., Bristow, S. L., & Haase, S. B. (2014). Analyzing transcription dynamics during the budding yeast cell cycle. Methods in Molecular Biology, 1170, 295–312. https://doi.org/10.1007/978-1-4939-888-2_14
Leman, A. R., S. L. Bristow, and S. B. Haase. “Analyzing transcription dynamics during the budding yeast cell cycle.” Methods in Molecular Biology 1170 (January 1, 2014): 295–312. https://doi.org/10.1007/978-1-4939-888-2_14.
Leman AR, Bristow SL, Haase SB. Analyzing transcription dynamics during the budding yeast cell cycle. Methods in Molecular Biology. 2014 Jan 1;1170:295–312.
Leman, A. R., et al. “Analyzing transcription dynamics during the budding yeast cell cycle.” Methods in Molecular Biology, vol. 1170, Jan. 2014, pp. 295–312. Scopus, doi:10.1007/978-1-4939-888-2_14.
Leman AR, Bristow SL, Haase SB. Analyzing transcription dynamics during the budding yeast cell cycle. Methods in Molecular Biology. 2014 Jan 1;1170:295–312.
Published In
Methods in Molecular Biology
DOI
ISSN
1064-3745
Publication Date
January 1, 2014
Volume
1170
Start / End Page
295 / 312
Related Subject Headings
- Developmental Biology
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences