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Sonic hedgehog from pharyngeal arch 1 epithelium is necessary for early mandibular arch cell survival and later cartilage condensation differentiation.

Publication ,  Journal Article
Billmyre, KK; Klingensmith, J
Published in: Dev Dyn
April 2015

BACKGROUND: Morphogenesis of vertebrate craniofacial skeletal elements is dependent on a key cell population, the cranial neural crest cells (NCC). Cranial NCC are formed dorsally in the cranial neural tube and migrate ventrally to form craniofacial skeletal elements as well as other tissues. Multiple extracellular signaling pathways regulate the migration, survival, proliferation, and differentiation of NCC. RESULTS: In this study, we demonstrate that Shh expression in the oral ectoderm and pharyngeal endoderm is essential for mandibular development. We show that a loss of Shh in these domains results in increased mesenchymal cell death in pharyngeal arch 1 (PA1) after NCC migration. This increased cell death can be rescued in utero by pharmacological inhibition of p53. Furthermore, we show that epithelial SHH is necessary for the early differentiation of mandibular cartilage condensations and, therefore, the subsequent development of Meckel's cartilage, around which the dentary bone forms. Nonetheless, a rescue of the cell death phenotype does not rescue the defect in cartilage condensation formation. CONCLUSIONS: Our results show that SHH produced by the PA1 epithelium is necessary for the survival of post-migratory NCC, and suggests a key role in the subsequent differentiation of chondrocytes to form Meckel's cartilage.

Duke Scholars

Published In

Dev Dyn

DOI

EISSN

1097-0177

Publication Date

April 2015

Volume

244

Issue

4

Start / End Page

564 / 576

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Transcription Factors
  • Signal Transduction
  • Phenotype
  • Pharynx
  • Neural Crest
  • Micrognathism
  • Mice, Transgenic
  • Mice
  • Mesoderm
 

Citation

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Chicago
ICMJE
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Billmyre, K. K., & Klingensmith, J. (2015). Sonic hedgehog from pharyngeal arch 1 epithelium is necessary for early mandibular arch cell survival and later cartilage condensation differentiation. Dev Dyn, 244(4), 564–576. https://doi.org/10.1002/dvdy.24256
Billmyre, Katherine Kretovich, and John Klingensmith. “Sonic hedgehog from pharyngeal arch 1 epithelium is necessary for early mandibular arch cell survival and later cartilage condensation differentiation.Dev Dyn 244, no. 4 (April 2015): 564–76. https://doi.org/10.1002/dvdy.24256.
Billmyre, Katherine Kretovich, and John Klingensmith. “Sonic hedgehog from pharyngeal arch 1 epithelium is necessary for early mandibular arch cell survival and later cartilage condensation differentiation.Dev Dyn, vol. 244, no. 4, Apr. 2015, pp. 564–76. Pubmed, doi:10.1002/dvdy.24256.
Journal cover image

Published In

Dev Dyn

DOI

EISSN

1097-0177

Publication Date

April 2015

Volume

244

Issue

4

Start / End Page

564 / 576

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Transcription Factors
  • Signal Transduction
  • Phenotype
  • Pharynx
  • Neural Crest
  • Micrognathism
  • Mice, Transgenic
  • Mice
  • Mesoderm