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Depletion of arterial L-arginine causes reversible tolerance to endothelium-dependent relaxation.

Publication ,  Journal Article
Gold, ME; Bush, PA; Ignarro, LJ
Published in: Biochemical and Biophysical Research Communications
October 1989

This study examined the influence of lowered arterial levels of L-arginine on endothelium-dependent relaxation of isolated rings of bovine pulmonary artery. Incubation of arterial rings under tension for 24 hr in oxygenated Krebs bicarbonate solution at 37 degrees C resulted in the development of marked or complete tolerance to A23187 (calcium ionophore)- and acetylcholine-elicited relaxation. Relaxant responses to nitric oxide were unaffected. Addition of L-arginine did not relax control rings but did elicit marked endothelium-dependent relaxation of tolerant rings that was inhibited by oxyhemoglobin or methylene blue. L-Arginine also restored acetylcholine-elicited relaxation. Inclusion of L-canavanine in the 24 hr incubations protected against the development of tolerance. The tissue concentration of arginine was 3-fold lower in tolerant than control arterial rings and L-canavanine restored arterial arginine levels to control values. Therefore, depletion of arterial L-arginine causes reversible tolerance to endothelium-dependent relaxation.

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Published In

Biochemical and Biophysical Research Communications

DOI

EISSN

1090-2104

ISSN

0006-291X

Publication Date

October 1989

Volume

164

Issue

2

Start / End Page

714 / 721

Related Subject Headings

  • Pulmonary Artery
  • Oxyhemoglobins
  • Nitric Oxide
  • Muscle, Smooth, Vascular
  • Muscle Relaxation
  • Muscle Contraction
  • Kinetics
  • Isomerism
  • In Vitro Techniques
  • Endothelium, Vascular
 

Citation

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ICMJE
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Gold, M. E., Bush, P. A., & Ignarro, L. J. (1989). Depletion of arterial L-arginine causes reversible tolerance to endothelium-dependent relaxation. Biochemical and Biophysical Research Communications, 164(2), 714–721. https://doi.org/10.1016/0006-291x(89)91518-0
Gold, M. E., P. A. Bush, and L. J. Ignarro. “Depletion of arterial L-arginine causes reversible tolerance to endothelium-dependent relaxation.Biochemical and Biophysical Research Communications 164, no. 2 (October 1989): 714–21. https://doi.org/10.1016/0006-291x(89)91518-0.
Gold ME, Bush PA, Ignarro LJ. Depletion of arterial L-arginine causes reversible tolerance to endothelium-dependent relaxation. Biochemical and Biophysical Research Communications. 1989 Oct;164(2):714–21.
Gold, M. E., et al. “Depletion of arterial L-arginine causes reversible tolerance to endothelium-dependent relaxation.Biochemical and Biophysical Research Communications, vol. 164, no. 2, Oct. 1989, pp. 714–21. Epmc, doi:10.1016/0006-291x(89)91518-0.
Gold ME, Bush PA, Ignarro LJ. Depletion of arterial L-arginine causes reversible tolerance to endothelium-dependent relaxation. Biochemical and Biophysical Research Communications. 1989 Oct;164(2):714–721.
Journal cover image

Published In

Biochemical and Biophysical Research Communications

DOI

EISSN

1090-2104

ISSN

0006-291X

Publication Date

October 1989

Volume

164

Issue

2

Start / End Page

714 / 721

Related Subject Headings

  • Pulmonary Artery
  • Oxyhemoglobins
  • Nitric Oxide
  • Muscle, Smooth, Vascular
  • Muscle Relaxation
  • Muscle Contraction
  • Kinetics
  • Isomerism
  • In Vitro Techniques
  • Endothelium, Vascular