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Functional repeats (TGYCC)n in the p53-inducible gene 3 (PIG3) promoter and susceptibility to squamous cell carcinoma of the head and neck.

Publication ,  Journal Article
Guan, X; Liu, Z; Wang, L; Wang, L-E; Sturgis, EM; Wei, Q
Published in: Carcinogenesis
April 2013

A polymorphic pentanucleotide microsatellite sequence (TGYCC)n within the p53-inducible gene 3 (PIG3) promoter is correlated with the extent of transcriptional activation by p53 and thought to modulate susceptibility to cancer. Using a PCR-silver staining-based single-strand conformation assay, we visualized variant genotypes of the PIG3 promoter (TGYCC)n motif in a subset of 100 subjects for each of four ethnic groups: non-Hispanic whites, African Americans, Hispanic Americans and Native Chinese. We found that PIG3 (TGYCC)15 was the most common allele but less frequent in non-Hispanic whites (0.660) than in Chinese (0.785) (P = 0.016). In an additional study of 616 patients with squamous cell carcinoma of the head and neck (SCCHN) and 623 cancer-free controls in a non-Hispanic white population, we found that compared with those who were PIG3 (TGYCC)15 homozygotes, subjects without the PIG3 (TGYCC)15 allele had a significantly increased SCCHN risk [adjusted odds ratio (OR) = 1.34; 95% CI = 1.04-1.73 for heterozygotes and OR = 1.69; 95% CI = 1.18-2.44 for variant homozygotes] in an allele-dose response manner (P = 0.002). Consistently, subsequent luciferase reporter assay revealed that the wild-type (TGYCC)15 allele had the highest p53-mediated transcriptional activity, compared with the other (TGYCC)n motifs. Our data suggest that the PIG3 variant polymorphic repeats alleles other than (TGYCC)15 may affect p53 binding and thus may be a marker for susceptibility to SCCHN, but our findings need to be validated in larger studies.

Duke Scholars

Published In

Carcinogenesis

DOI

EISSN

1460-2180

Publication Date

April 2013

Volume

34

Issue

4

Start / End Page

812 / 817

Location

England

Related Subject Headings

  • White People
  • Transcriptional Activation
  • Squamous Cell Carcinoma of Head and Neck
  • Proto-Oncogene Proteins
  • Promoter Regions, Genetic
  • Polymorphism, Single Nucleotide
  • Oncology & Carcinogenesis
  • Middle Aged
  • Microsatellite Repeats
  • Male
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Guan, X., Liu, Z., Wang, L., Wang, L.-E., Sturgis, E. M., & Wei, Q. (2013). Functional repeats (TGYCC)n in the p53-inducible gene 3 (PIG3) promoter and susceptibility to squamous cell carcinoma of the head and neck. Carcinogenesis, 34(4), 812–817. https://doi.org/10.1093/carcin/bgs388
Guan, Xiaoxiang, Zhensheng Liu, Luo Wang, Li-E Wang, Erich M. Sturgis, and Qingyi Wei. “Functional repeats (TGYCC)n in the p53-inducible gene 3 (PIG3) promoter and susceptibility to squamous cell carcinoma of the head and neck.Carcinogenesis 34, no. 4 (April 2013): 812–17. https://doi.org/10.1093/carcin/bgs388.
Guan, Xiaoxiang, et al. “Functional repeats (TGYCC)n in the p53-inducible gene 3 (PIG3) promoter and susceptibility to squamous cell carcinoma of the head and neck.Carcinogenesis, vol. 34, no. 4, Apr. 2013, pp. 812–17. Pubmed, doi:10.1093/carcin/bgs388.
Guan X, Liu Z, Wang L, Wang L-E, Sturgis EM, Wei Q. Functional repeats (TGYCC)n in the p53-inducible gene 3 (PIG3) promoter and susceptibility to squamous cell carcinoma of the head and neck. Carcinogenesis. 2013 Apr;34(4):812–817.
Journal cover image

Published In

Carcinogenesis

DOI

EISSN

1460-2180

Publication Date

April 2013

Volume

34

Issue

4

Start / End Page

812 / 817

Location

England

Related Subject Headings

  • White People
  • Transcriptional Activation
  • Squamous Cell Carcinoma of Head and Neck
  • Proto-Oncogene Proteins
  • Promoter Regions, Genetic
  • Polymorphism, Single Nucleotide
  • Oncology & Carcinogenesis
  • Middle Aged
  • Microsatellite Repeats
  • Male