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Microbiota regulate intestinal absorption and metabolism of fatty acids in the zebrafish.

Publication ,  Journal Article
Semova, I; Carten, JD; Stombaugh, J; Mackey, LC; Knight, R; Farber, SA; Rawls, JF
Published in: Cell Host Microbe
September 13, 2012

Regulation of intestinal dietary fat absorption is critical to maintaining energy balance. While intestinal microbiota clearly impact the host's energy balance, their role in intestinal absorption and extraintestinal metabolism of dietary fat is less clear. Using in vivo imaging of fluorescent fatty acid (FA) analogs delivered to gnotobiotic zebrafish hosts, we reveal that microbiota stimulate FA uptake and lipid droplet (LD) formation in the intestinal epithelium and liver. Microbiota increase epithelial LD number in a diet-dependent manner. The presence of food led to the intestinal enrichment of bacteria from the phylum Firmicutes. Diet-enriched Firmicutes and their products were sufficient to increase epithelial LD number, whereas LD size was increased by other bacterial types. Thus, different members of the intestinal microbiota promote FA absorption via distinct mechanisms. Diet-induced alterations in microbiota composition might influence fat absorption, providing mechanistic insight into how microbiota-diet interactions regulate host energy balance.

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Published In

Cell Host Microbe

DOI

EISSN

1934-6069

Publication Date

September 13, 2012

Volume

12

Issue

3

Start / End Page

277 / 288

Location

United States

Related Subject Headings

  • Zebrafish
  • Metagenome
  • Liver
  • Intestines
  • Intestinal Mucosa
  • Intestinal Absorption
  • Immunology
  • Image Processing, Computer-Assisted
  • Fluorescence
  • Fatty Acids
 

Citation

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Semova, I., Carten, J. D., Stombaugh, J., Mackey, L. C., Knight, R., Farber, S. A., & Rawls, J. F. (2012). Microbiota regulate intestinal absorption and metabolism of fatty acids in the zebrafish. Cell Host Microbe, 12(3), 277–288. https://doi.org/10.1016/j.chom.2012.08.003
Semova, Ivana, Juliana D. Carten, Jesse Stombaugh, Lantz C. Mackey, Rob Knight, Steven A. Farber, and John F. Rawls. “Microbiota regulate intestinal absorption and metabolism of fatty acids in the zebrafish.Cell Host Microbe 12, no. 3 (September 13, 2012): 277–88. https://doi.org/10.1016/j.chom.2012.08.003.
Semova I, Carten JD, Stombaugh J, Mackey LC, Knight R, Farber SA, et al. Microbiota regulate intestinal absorption and metabolism of fatty acids in the zebrafish. Cell Host Microbe. 2012 Sep 13;12(3):277–88.
Semova, Ivana, et al. “Microbiota regulate intestinal absorption and metabolism of fatty acids in the zebrafish.Cell Host Microbe, vol. 12, no. 3, Sept. 2012, pp. 277–88. Pubmed, doi:10.1016/j.chom.2012.08.003.
Semova I, Carten JD, Stombaugh J, Mackey LC, Knight R, Farber SA, Rawls JF. Microbiota regulate intestinal absorption and metabolism of fatty acids in the zebrafish. Cell Host Microbe. 2012 Sep 13;12(3):277–288.

Published In

Cell Host Microbe

DOI

EISSN

1934-6069

Publication Date

September 13, 2012

Volume

12

Issue

3

Start / End Page

277 / 288

Location

United States

Related Subject Headings

  • Zebrafish
  • Metagenome
  • Liver
  • Intestines
  • Intestinal Mucosa
  • Intestinal Absorption
  • Immunology
  • Image Processing, Computer-Assisted
  • Fluorescence
  • Fatty Acids