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In vivo analysis of white adipose tissue in zebrafish.

Publication ,  Journal Article
Minchin, JEN; Rawls, JF
Published in: Methods Cell Biol
2011

White adipose tissue (WAT) is the major site of energy storage in bony vertebrates, and also serves central roles in the endocrine regulation of energy balance. The cellular and molecular mechanisms underlying WAT development and physiology are not well understood. This is due in part to difficulties associated with imaging adipose tissues in mammalian model systems, especially during early life stages. The zebrafish (Danio rerio) has recently emerged as a new model system for adipose tissue research, in which WAT can be imaged in a transparent living vertebrate at all life stages. Here we present detailed methods for labeling adipocytes in live zebrafish using fluorescent lipophilic dyes, and for in vivo microscopy of zebrafish WAT.

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Published In

Methods Cell Biol

DOI

ISSN

0091-679X

Publication Date

2011

Volume

105

Start / End Page

63 / 86

Location

United States

Related Subject Headings

  • Zebrafish
  • Staining and Labeling
  • Molecular Imaging
  • Microscopy, Fluorescence
  • Male
  • Lipids
  • Larva
  • Fluorescent Dyes
  • Fixatives
  • Female
 

Citation

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Minchin, J. E. N., & Rawls, J. F. (2011). In vivo analysis of white adipose tissue in zebrafish. Methods Cell Biol, 105, 63–86. https://doi.org/10.1016/B978-0-12-381320-6.00003-5
Minchin, James E. N., and John F. Rawls. “In vivo analysis of white adipose tissue in zebrafish.Methods Cell Biol 105 (2011): 63–86. https://doi.org/10.1016/B978-0-12-381320-6.00003-5.
Minchin JEN, Rawls JF. In vivo analysis of white adipose tissue in zebrafish. Methods Cell Biol. 2011;105:63–86.
Minchin, James E. N., and John F. Rawls. “In vivo analysis of white adipose tissue in zebrafish.Methods Cell Biol, vol. 105, 2011, pp. 63–86. Pubmed, doi:10.1016/B978-0-12-381320-6.00003-5.
Minchin JEN, Rawls JF. In vivo analysis of white adipose tissue in zebrafish. Methods Cell Biol. 2011;105:63–86.
Journal cover image

Published In

Methods Cell Biol

DOI

ISSN

0091-679X

Publication Date

2011

Volume

105

Start / End Page

63 / 86

Location

United States

Related Subject Headings

  • Zebrafish
  • Staining and Labeling
  • Molecular Imaging
  • Microscopy, Fluorescence
  • Male
  • Lipids
  • Larva
  • Fluorescent Dyes
  • Fixatives
  • Female