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Methods for generating and colonizing gnotobiotic zebrafish.

Publication ,  Journal Article
Pham, LN; Kanther, M; Semova, I; Rawls, JF
Published in: Nat Protoc
2008

Vertebrates are colonized at birth by complex and dynamic communities of microorganisms that can contribute significantly to host health and disease. The ability to raise animals in the absence of microorganisms has been a powerful tool for elucidating the relationships between animal hosts and their microbial residents. The optical transparency of the developing zebrafish and relative ease of generating germ-free (GF) zebrafish make it an attractive model organism for gnotobiotic research. Here we provide a protocol for generating zebrafish embryos; deriving and rearing GF zebrafish; and colonizing zebrafish with microorganisms. Using these methods, we typically obtain 80-90% sterility rates in our GF derivations with 90% survival in GF animals and 50-90% survival in colonized animals through larval stages. Obtaining embryos for derivation requires approximately 1-2 h, with a 3- to 8-h incubation period before derivation. Derivation of GF animals takes 1-1.5 h, and daily maintenance requires 1-2 h.

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Published In

Nat Protoc

DOI

EISSN

1750-2799

Publication Date

2008

Volume

3

Issue

12

Start / End Page

1862 / 1875

Location

England

Related Subject Headings

  • Zebrafish
  • Laboratory Animal Science
  • Housing, Animal
  • Germ-Free Life
  • Embryo, Nonmammalian
  • Breeding
  • Bioinformatics
  • Animals
  • 11 Medical and Health Sciences
  • 06 Biological Sciences
 

Citation

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Pham, L. N., Kanther, M., Semova, I., & Rawls, J. F. (2008). Methods for generating and colonizing gnotobiotic zebrafish. Nat Protoc, 3(12), 1862–1875. https://doi.org/10.1038/nprot.2008.186
Pham, Linh N., Michelle Kanther, Ivana Semova, and John F. Rawls. “Methods for generating and colonizing gnotobiotic zebrafish.Nat Protoc 3, no. 12 (2008): 1862–75. https://doi.org/10.1038/nprot.2008.186.
Pham LN, Kanther M, Semova I, Rawls JF. Methods for generating and colonizing gnotobiotic zebrafish. Nat Protoc. 2008;3(12):1862–75.
Pham, Linh N., et al. “Methods for generating and colonizing gnotobiotic zebrafish.Nat Protoc, vol. 3, no. 12, 2008, pp. 1862–75. Pubmed, doi:10.1038/nprot.2008.186.
Pham LN, Kanther M, Semova I, Rawls JF. Methods for generating and colonizing gnotobiotic zebrafish. Nat Protoc. 2008;3(12):1862–1875.

Published In

Nat Protoc

DOI

EISSN

1750-2799

Publication Date

2008

Volume

3

Issue

12

Start / End Page

1862 / 1875

Location

England

Related Subject Headings

  • Zebrafish
  • Laboratory Animal Science
  • Housing, Animal
  • Germ-Free Life
  • Embryo, Nonmammalian
  • Breeding
  • Bioinformatics
  • Animals
  • 11 Medical and Health Sciences
  • 06 Biological Sciences