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Definition of a functional single nucleotide polymorphism in the cell migration inhibitory gene MIIP that affects the risk of breast cancer.

Publication ,  Journal Article
Song, F; Ji, P; Zheng, H; Song, F; Wang, Y; Hao, X; Wei, Q; Zhang, W; Chen, K
Published in: Cancer Res
February 1, 2010

The migration and invasion inhibitory protein MIIP is an inhibitor of cancer cell migration and invasion that inhibits breast tumorigenesis. In this case-control study, we evaluated the MIIP single nucleotide polymorphism (SNP) rs2295283 (codon 167, A>G, K>E) from 1,524 breast cancer patients and 1,592 age-matched controls for its association with breast cancer risk. SNP analysis included a validation set of 736 cases and 760 controls. Colony formation and cell migration assays were then conducted to functionally interrogate the genotype difference. When compared with the AA genotype, the combined AG + GG genotypes (167E) were associated with a significantly lower risk of breast cancer. In the test set, the protective effects of the AG + GG genotypes were more evident among participants with a family history of cancer. Further case series analysis revealed that the GG genotype was associated with reduced breast cancer susceptibility in cases of tumor size >2 cm and late clinical stage (II + III + IV). Colony formation assays showed that MIIP 167E (the G variant) was a more potent inhibitor of colony formation but not cell migration. These results suggest MIIP K167E as a functional genetic marker of breast cancer development and prognosis.

Duke Scholars

Published In

Cancer Res

DOI

EISSN

1538-7445

Publication Date

February 1, 2010

Volume

70

Issue

3

Start / End Page

1024 / 1032

Location

United States

Related Subject Headings

  • Risk Factors
  • Prognosis
  • Polymorphism, Single Nucleotide
  • Phenotype
  • Oncology & Carcinogenesis
  • Multivariate Analysis
  • Middle Aged
  • Logistic Models
  • Linkage Disequilibrium
  • Intracellular Signaling Peptides and Proteins
 

Citation

APA
Chicago
ICMJE
MLA
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Song, F., Ji, P., Zheng, H., Wang, Y., Hao, X., Wei, Q., … Chen, K. (2010). Definition of a functional single nucleotide polymorphism in the cell migration inhibitory gene MIIP that affects the risk of breast cancer. Cancer Res, 70(3), 1024–1032. https://doi.org/10.1158/0008-5472.CAN-09-3742
Song, Fangfang, Ping Ji, Hong Zheng, Fengju Song, Yingmei Wang, Xishan Hao, Qingyi Wei, Wei Zhang, and Kexin Chen. “Definition of a functional single nucleotide polymorphism in the cell migration inhibitory gene MIIP that affects the risk of breast cancer.Cancer Res 70, no. 3 (February 1, 2010): 1024–32. https://doi.org/10.1158/0008-5472.CAN-09-3742.
Song, Fangfang, et al. “Definition of a functional single nucleotide polymorphism in the cell migration inhibitory gene MIIP that affects the risk of breast cancer.Cancer Res, vol. 70, no. 3, Feb. 2010, pp. 1024–32. Pubmed, doi:10.1158/0008-5472.CAN-09-3742.
Song F, Ji P, Zheng H, Wang Y, Hao X, Wei Q, Zhang W, Chen K. Definition of a functional single nucleotide polymorphism in the cell migration inhibitory gene MIIP that affects the risk of breast cancer. Cancer Res. 2010 Feb 1;70(3):1024–1032.

Published In

Cancer Res

DOI

EISSN

1538-7445

Publication Date

February 1, 2010

Volume

70

Issue

3

Start / End Page

1024 / 1032

Location

United States

Related Subject Headings

  • Risk Factors
  • Prognosis
  • Polymorphism, Single Nucleotide
  • Phenotype
  • Oncology & Carcinogenesis
  • Multivariate Analysis
  • Middle Aged
  • Logistic Models
  • Linkage Disequilibrium
  • Intracellular Signaling Peptides and Proteins