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Expression of nucleotide excision repair genes and the risk for squamous cell carcinoma of the head and neck.

Publication ,  Journal Article
Cheng, L; Sturgis, EM; Eicher, SA; Spitz, MR; Wei, Q
Published in: Cancer
January 15, 2002

BACKGROUND: Phenotypic differences in the ability to repair genetic damage induced by tobacco carcinogens may reflect genetic differences in susceptibility to squamous cell carcinoma of the head and neck (SCCHN). The objective of this study was to assess the variation in baseline expression of five nucleotide excision repair genes between individuals with SCCHN and cancer free controls. METHODS: The authors conducted a hospital-based case-control study of 57 SCCHN patients and 105 cancer free controls. Using peripheral blood lymphocytes, a multiplex reverse transcriptase-polymerase chain reaction assay was used to quantitate in vitro the mRNA levels of five genes (ERCC1, XPB/ERCC3, XPG/ERCC5, CSB/ERCC6, and XPC) involved in the nucleotide excision repair pathway. RESULTS: The levels of ERCC1, XPB/ERCC3, XPG/ERCC5, and CSB/ERCC6 transcripts were lower in cases than in controls (P =0.0001, 0.096, 0.001, and 0.0001, respectively). In multivariate logistic regression analysis (adjusting for age, gender, race, smoking status, and alcohol use), low expression of ERCC1, XPB/ERCC3, XPG/ERCC5, and CSB/ERCC6 was associated with a statistically significant increased risk for SCCHN (adjusted odds ratios [95% confidence intervals] 6.42 [2.63-15.69], 2.86 [1.39-5.90], 3.69 [1.73-7.90], and 2.46 [1.19-5.09], respectively). CONCLUSIONS: Reduced expression of ERCC1, XPB/ERCC3, XPG/ERCC5, and CSB/ERCC6 is associated with a more than two-fold increased risk of SCCHN.

Duke Scholars

Published In

Cancer

DOI

ISSN

0008-543X

Publication Date

January 15, 2002

Volume

94

Issue

2

Start / End Page

393 / 397

Location

United States

Related Subject Headings

  • Transcription Factors
  • Risk Factors
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Proteins
  • Poly-ADP-Ribose Binding Proteins
  • Pilot Projects
  • Oncology & Carcinogenesis
  • Nuclear Proteins
  • Middle Aged
 

Citation

APA
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ICMJE
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Cheng, L., Sturgis, E. M., Eicher, S. A., Spitz, M. R., & Wei, Q. (2002). Expression of nucleotide excision repair genes and the risk for squamous cell carcinoma of the head and neck. Cancer, 94(2), 393–397. https://doi.org/10.1002/cncr.10231
Cheng, Lie, Erich M. Sturgis, Susan A. Eicher, Margaret R. Spitz, and Qingyi Wei. “Expression of nucleotide excision repair genes and the risk for squamous cell carcinoma of the head and neck.Cancer 94, no. 2 (January 15, 2002): 393–97. https://doi.org/10.1002/cncr.10231.
Cheng L, Sturgis EM, Eicher SA, Spitz MR, Wei Q. Expression of nucleotide excision repair genes and the risk for squamous cell carcinoma of the head and neck. Cancer. 2002 Jan 15;94(2):393–7.
Cheng, Lie, et al. “Expression of nucleotide excision repair genes and the risk for squamous cell carcinoma of the head and neck.Cancer, vol. 94, no. 2, Jan. 2002, pp. 393–97. Pubmed, doi:10.1002/cncr.10231.
Cheng L, Sturgis EM, Eicher SA, Spitz MR, Wei Q. Expression of nucleotide excision repair genes and the risk for squamous cell carcinoma of the head and neck. Cancer. 2002 Jan 15;94(2):393–397.
Journal cover image

Published In

Cancer

DOI

ISSN

0008-543X

Publication Date

January 15, 2002

Volume

94

Issue

2

Start / End Page

393 / 397

Location

United States

Related Subject Headings

  • Transcription Factors
  • Risk Factors
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Proteins
  • Poly-ADP-Ribose Binding Proteins
  • Pilot Projects
  • Oncology & Carcinogenesis
  • Nuclear Proteins
  • Middle Aged