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Reduced expression of mismatch repair genes measured by multiplex reverse transcription-polymerase chain reaction in human gliomas.

Publication ,  Journal Article
Wei, Q; Bondy, ML; Mao, L; Gaun, Y; Cheng, L; Cunningham, J; Fan, Y; Bruner, JM; Yung, WK; Levin, VA; Kyritsis, AP
Published in: Cancer Res
May 1, 1997

Microsatellite instability (MIN) is frequently observed in hereditary nonpolyposis colon cancer and in other sporadic cancers including gliomas. Abnormalities in at least one of five mismatch repair (MMR) genes are implicated in the development of cancers in hereditary nonpolyposis colon cancer and the associated MIN. Using a newly developed multiplex reverse transcription-PCR assay, we evaluated the expression of the five known human MMR genes (hMSH2, hMLH1, hPMS1, hPMS2, and GTBP) in human gliomas by measuring simultaneously the relative levels of the transcripts. The beta-actin gene was used as an internal control for RNA degradation and DNA contamination and as a reference for quantifying the levels of their transcripts. Of the 33 gliomas examined, 42% (14) had low expression of hMSH2 (at least 4-5-fold lower than normal mean), 21% (7) had low expression of hMLH1, and 18% (6) had low expression of hPMS1 compared with the expression in the lymphocytes from 13 normal individuals. Furthermore, six of the 33 (18%) tumor samples had decreased expression of more than one MMR gene. Two of these six patients with multiple gene abnormalities had second primary cancers, and an additional patient had multifocal gliomas. Further molecular analysis of available DNA samples indicated that one of five of those tumors with aberrant expression of MMR genes had MIN, as compared with none of five tumors with normal expression. These data suggest that reduced expression of MMR genes is frequent in human gliomas and that aberrant expression of more than one MMR gene may be associated with increased risk of second primary malignancies in glioma patients.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

May 1, 1997

Volume

57

Issue

9

Start / End Page

1673 / 1677

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae Proteins
  • RNA, Neoplasm
  • RNA, Messenger
  • Proteins
  • Polymerase Chain Reaction
  • Oncology & Carcinogenesis
  • Neoplasm Proteins
  • MutS Homolog 2 Protein
  • MutL Proteins
  • MutL Protein Homolog 1
 

Citation

APA
Chicago
ICMJE
MLA
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Wei, Q., Bondy, M. L., Mao, L., Gaun, Y., Cheng, L., Cunningham, J., … Kyritsis, A. P. (1997). Reduced expression of mismatch repair genes measured by multiplex reverse transcription-polymerase chain reaction in human gliomas. Cancer Res, 57(9), 1673–1677.
Wei, Q., M. L. Bondy, L. Mao, Y. Gaun, L. Cheng, J. Cunningham, Y. Fan, et al. “Reduced expression of mismatch repair genes measured by multiplex reverse transcription-polymerase chain reaction in human gliomas.Cancer Res 57, no. 9 (May 1, 1997): 1673–77.
Wei Q, Bondy ML, Mao L, Gaun Y, Cheng L, Cunningham J, et al. Reduced expression of mismatch repair genes measured by multiplex reverse transcription-polymerase chain reaction in human gliomas. Cancer Res. 1997 May 1;57(9):1673–7.
Wei Q, Bondy ML, Mao L, Gaun Y, Cheng L, Cunningham J, Fan Y, Bruner JM, Yung WK, Levin VA, Kyritsis AP. Reduced expression of mismatch repair genes measured by multiplex reverse transcription-polymerase chain reaction in human gliomas. Cancer Res. 1997 May 1;57(9):1673–1677.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

May 1, 1997

Volume

57

Issue

9

Start / End Page

1673 / 1677

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae Proteins
  • RNA, Neoplasm
  • RNA, Messenger
  • Proteins
  • Polymerase Chain Reaction
  • Oncology & Carcinogenesis
  • Neoplasm Proteins
  • MutS Homolog 2 Protein
  • MutL Proteins
  • MutL Protein Homolog 1