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Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology.

Publication ,  Journal Article
Barker, NM; Carrino, DA; Caplan, AI; Hurd, WW; Liu, JH; Tan, H; Mesiano, S
Published in: Reprod Sci
March 2016

Uterine leiomyoma are a common benign pelvic tumors composed of modified smooth muscle cells and a large amount of extracellular matrix (ECM). The proteoglycan composition of the leiomyoma ECM is thought to affect pathophysiology of the disease. To test this hypothesis, we examined the abundance (by immunoblotting) and expression (by quantitative real-time polymerase chain reaction) of the proteoglycans biglycan, decorin, and versican in leiomyoma and normal myometrium and determined whether expression is affected by steroid hormones and menstrual phase. Leiomyoma and normal myometrium were collected from women (n = 17) undergoing hysterectomy or myomectomy. In vitro studies were performed on immortalized leiomyoma (UtLM) and normal myometrial (hTERT-HM) cells with and without exposure to estradiol and progesterone. In leiomyoma tissue, abundance of decorin messenger RNA (mRNA) and protein were 2.6-fold and 1.4-fold lower, respectively, compared with normal myometrium. Abundance of versican mRNA was not different between matched samples, whereas versican protein was increased 1.8-fold in leiomyoma compared with myometrium. Decorin mRNA was 2.4-fold lower in secretory phase leiomyoma compared with proliferative phase tissue. In UtLM cells, progesterone decreased the abundance of decorin mRNA by 1.3-fold. Lower decorin expression in leiomyoma compared with myometrium may contribute to disease growth and progression. As decorin inhibits the activity of specific growth factors, its reduced level in the leiomyoma cell microenvironment may promote cell proliferation and ECM deposition. Our data suggest that decorin expression in leiomyoma is inhibited by progesterone, which may be a mechanism by which the ovarian steroids affect leiomyoma growth and disease progression.

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Published In

Reprod Sci

DOI

EISSN

1933-7205

Publication Date

March 2016

Volume

23

Issue

3

Start / End Page

302 / 309

Location

United States

Related Subject Headings

  • Uterine Neoplasms
  • Proteoglycans
  • Promegestone
  • Progesterone
  • Obstetrics & Reproductive Medicine
  • Myometrium
  • Middle Aged
  • Leiomyoma
  • Humans
  • Female
 

Citation

APA
Chicago
ICMJE
MLA
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Barker, N. M., Carrino, D. A., Caplan, A. I., Hurd, W. W., Liu, J. H., Tan, H., & Mesiano, S. (2016). Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology. Reprod Sci, 23(3), 302–309. https://doi.org/10.1177/1933719115607994
Barker, Nichole M., David A. Carrino, Arnold I. Caplan, William W. Hurd, James H. Liu, Huiqing Tan, and Sam Mesiano. “Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology.Reprod Sci 23, no. 3 (March 2016): 302–9. https://doi.org/10.1177/1933719115607994.
Barker NM, Carrino DA, Caplan AI, Hurd WW, Liu JH, Tan H, et al. Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology. Reprod Sci. 2016 Mar;23(3):302–9.
Barker, Nichole M., et al. “Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology.Reprod Sci, vol. 23, no. 3, Mar. 2016, pp. 302–09. Pubmed, doi:10.1177/1933719115607994.
Barker NM, Carrino DA, Caplan AI, Hurd WW, Liu JH, Tan H, Mesiano S. Proteoglycans in Leiomyoma and Normal Myometrium: Abundance, Steroid Hormone Control, and Implications for Pathophysiology. Reprod Sci. 2016 Mar;23(3):302–309.
Journal cover image

Published In

Reprod Sci

DOI

EISSN

1933-7205

Publication Date

March 2016

Volume

23

Issue

3

Start / End Page

302 / 309

Location

United States

Related Subject Headings

  • Uterine Neoplasms
  • Proteoglycans
  • Promegestone
  • Progesterone
  • Obstetrics & Reproductive Medicine
  • Myometrium
  • Middle Aged
  • Leiomyoma
  • Humans
  • Female