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Halogenated carbazoles induce cardiotoxicity in developing zebrafish (Danio rerio) embryos.

Publication ,  Journal Article
Fang, M; Guo, J; Chen, D; Li, A; Hinton, DE; Dong, W
Published in: Environmental toxicology and chemistry
October 2016

Halogenated carbazoles are increasingly identified as a novel class of environmental contaminants. However, no in vivo acute toxicity information on those compounds was available. In the present study, an in vivo zebrafish embryonic model (Danio rerio) was used to investigate the developmental toxicity of those halogenated carbazoles. The results suggested that acute toxicity was structure-dependent. Two of the 6 tested carbazoles, 2,7-dibromocarbazole (27-DBCZ) and 2,3,6,7-tetrachlorocarbazole, showed obvious developmental toxicity at nanomolar levels. The typical phenotypes were similar to dioxin-induced cardiotoxicity, including swollen yolk sac, pericardial sac edema, elongated and unlooped heart, and lower jaw shortening. During embryonic development 27-DBCZ also induced a unique pigmentation decrease. Gene expression and protein staining of cytochrome P4501A (CYP1A) showed that both halogenated carbazoles could induce CYP1A expression at the micromolar level and primarily in the heart area, which was similar to dioxin activity. Further, aryl hydrocarbon receptor-(AhR)2 gene knockdown with morpholino confirmed that the acute cardiotoxicity is AhR-dependent. In conclusion, the results demonstrate that halogenated carbazoles represent yet another class of persistent organic pollutants with dioxin-like activity in an in vivo animal model. Environ Toxicol Chem 2016;35:2523-2529. © 2016 SETAC.

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Published In

Environmental toxicology and chemistry

DOI

EISSN

1552-8618

ISSN

0730-7268

Publication Date

October 2016

Volume

35

Issue

10

Start / End Page

2523 / 2529

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Yolk Sac
  • Water Pollutants, Chemical
  • Structure-Activity Relationship
  • Receptors, Aryl Hydrocarbon
  • Hydrocarbons, Halogenated
  • Heart
  • Environmental Sciences
  • Embryonic Development
 

Citation

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Fang, M., Guo, J., Chen, D., Li, A., Hinton, D. E., & Dong, W. (2016). Halogenated carbazoles induce cardiotoxicity in developing zebrafish (Danio rerio) embryos. Environmental Toxicology and Chemistry, 35(10), 2523–2529. https://doi.org/10.1002/etc.3416
Fang, Mingliang, Jiehong Guo, Da Chen, An Li, David E. Hinton, and Wu Dong. “Halogenated carbazoles induce cardiotoxicity in developing zebrafish (Danio rerio) embryos.Environmental Toxicology and Chemistry 35, no. 10 (October 2016): 2523–29. https://doi.org/10.1002/etc.3416.
Fang M, Guo J, Chen D, Li A, Hinton DE, Dong W. Halogenated carbazoles induce cardiotoxicity in developing zebrafish (Danio rerio) embryos. Environmental toxicology and chemistry. 2016 Oct;35(10):2523–9.
Fang, Mingliang, et al. “Halogenated carbazoles induce cardiotoxicity in developing zebrafish (Danio rerio) embryos.Environmental Toxicology and Chemistry, vol. 35, no. 10, Oct. 2016, pp. 2523–29. Epmc, doi:10.1002/etc.3416.
Fang M, Guo J, Chen D, Li A, Hinton DE, Dong W. Halogenated carbazoles induce cardiotoxicity in developing zebrafish (Danio rerio) embryos. Environmental toxicology and chemistry. 2016 Oct;35(10):2523–2529.
Journal cover image

Published In

Environmental toxicology and chemistry

DOI

EISSN

1552-8618

ISSN

0730-7268

Publication Date

October 2016

Volume

35

Issue

10

Start / End Page

2523 / 2529

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Yolk Sac
  • Water Pollutants, Chemical
  • Structure-Activity Relationship
  • Receptors, Aryl Hydrocarbon
  • Hydrocarbons, Halogenated
  • Heart
  • Environmental Sciences
  • Embryonic Development