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Squamous cell carcinoma of the oral cavity often overexpresses p16 but is rarely driven by human papillomavirus.

Publication ,  Journal Article
Zafereo, ME; Xu, L; Dahlstrom, KR; Viamonte, CA; El-Naggar, AK; Wei, Q; Li, G; Sturgis, EM
Published in: Oral Oncol
May 2016

OBJECTIVE: Human papillomavirus (HPV) is a causal and prognostic factor for oropharyngeal cancer, but its role in squamous cell carcinoma of the oral cavity (SCCOC) is unclear. We sought to clarify HPV's role in SCCOC. MATERIALS AND METHODS: Patients with newly diagnosed SCCOC (N=460) were prospectively recruited, treated, and followed at one institution. p16/HPV status was determined by p16 immunohistochemistry (IHC) (N=210), PCR-based HPV 16/18 E6/7 DNA testing (N=403), and/or HPV in situ hybridization (ISH) (N=178). Kaplan-Meier curves and log-rank tests were used to compare survival by p16/HPV status. RESULTS: p16 expression was detected in 30% of tumors (62/210) and HPV 16/18 E6/7 DNA in 28% (114/403), although correlation between these two assays was poor (r=-0.01). Patients with p16-positive tumors were more likely to be younger and have primary tumors of the oral tongue. Only 4% of tumors (7/171) were positive for HPV by ISH. Comparisons of patients with p16-positive and p16-negative tumors, patients with HPV-positive and HPV-negative tumors by PCR, and patients with HPV-positive and HPV-negative tumors by ISH showed no significant differences in disease-specific or disease-free survival by p16/HPV status. When we applied a more stringent definition of HPV positivity based on a combination of assay results, only 10 of 166 tumors were HPV positive, and there were no significant differences in demographic, exposure, clinical, or survival characteristics between these patients and the 156 HPV-negative patients. CONCLUSIONS: Very few patients with SCCOC have HPV-driven tumors. SCCOC that overexpresses p16 may be a unique subset deserving of further study.

Duke Scholars

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Published In

Oral Oncol

DOI

EISSN

1879-0593

Publication Date

May 2016

Volume

56

Start / End Page

47 / 53

Location

England

Related Subject Headings

  • Oncology & Carcinogenesis
  • Mouth Neoplasms
  • Middle Aged
  • Male
  • Humans
  • Genes, p16
  • Female
  • Dentistry
  • Carcinoma, Squamous Cell
  • Alphapapillomavirus
 

Citation

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ICMJE
MLA
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Zafereo, M. E., Xu, L., Dahlstrom, K. R., Viamonte, C. A., El-Naggar, A. K., Wei, Q., … Sturgis, E. M. (2016). Squamous cell carcinoma of the oral cavity often overexpresses p16 but is rarely driven by human papillomavirus. Oral Oncol, 56, 47–53. https://doi.org/10.1016/j.oraloncology.2016.03.003
Zafereo, Mark E., Li Xu, Kristina R. Dahlstrom, Carlo A. Viamonte, Adel K. El-Naggar, Qingyi Wei, Guojun Li, and Erich M. Sturgis. “Squamous cell carcinoma of the oral cavity often overexpresses p16 but is rarely driven by human papillomavirus.Oral Oncol 56 (May 2016): 47–53. https://doi.org/10.1016/j.oraloncology.2016.03.003.
Zafereo ME, Xu L, Dahlstrom KR, Viamonte CA, El-Naggar AK, Wei Q, et al. Squamous cell carcinoma of the oral cavity often overexpresses p16 but is rarely driven by human papillomavirus. Oral Oncol. 2016 May;56:47–53.
Zafereo, Mark E., et al. “Squamous cell carcinoma of the oral cavity often overexpresses p16 but is rarely driven by human papillomavirus.Oral Oncol, vol. 56, May 2016, pp. 47–53. Pubmed, doi:10.1016/j.oraloncology.2016.03.003.
Zafereo ME, Xu L, Dahlstrom KR, Viamonte CA, El-Naggar AK, Wei Q, Li G, Sturgis EM. Squamous cell carcinoma of the oral cavity often overexpresses p16 but is rarely driven by human papillomavirus. Oral Oncol. 2016 May;56:47–53.

Published In

Oral Oncol

DOI

EISSN

1879-0593

Publication Date

May 2016

Volume

56

Start / End Page

47 / 53

Location

England

Related Subject Headings

  • Oncology & Carcinogenesis
  • Mouth Neoplasms
  • Middle Aged
  • Male
  • Humans
  • Genes, p16
  • Female
  • Dentistry
  • Carcinoma, Squamous Cell
  • Alphapapillomavirus