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Preconditioning Vaccine Sites for mRNA-Transfected Dendritic Cell Therapy and Antitumor Efficacy.

Publication ,  Chapter
Batich, KA; Swartz, AM; Sampson, JH
2016

Messenger RNA (mRNA)-transfected dendritic cell (DC) vaccines have been shown to be a powerful modality for eliciting antitumor immune responses in mice and humans; however, their application has not been fully optimized since many of the factors that contribute to their efficacy remain poorly understood. Work stemming from our laboratory has recently demonstrated that preconditioning the vaccine site with a recall antigen prior to the administration of a dendritic cell vaccine creates systemic recall responses and resultantly enhances dendritic cell migration to the lymph nodes with improved antitumor efficacy. This chapter describes the generation of murine mRNA-transfected DC vaccines, as well as a method for vaccine site preconditioning with protein antigen formulations that create potent recall responses.

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DOI

Publication Date

2016

Volume

1403

Start / End Page

819 / 838

Related Subject Headings

  • Vaccination
  • Transfection
  • Transcription, Genetic
  • RNA, Messenger
  • Phenotype
  • Neoplasms
  • Mice
  • Immunotherapy
  • Flow Cytometry
  • Electrophoresis
 

Citation

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Batich, K. A., Swartz, A. M., & Sampson, J. H. (2016). Preconditioning Vaccine Sites for mRNA-Transfected Dendritic Cell Therapy and Antitumor Efficacy. (Vol. 1403, pp. 819–838). https://doi.org/10.1007/978-1-4939-3387-7_47
Batich, Kristen A., Adam M. Swartz, and John H. Sampson. “Preconditioning Vaccine Sites for mRNA-Transfected Dendritic Cell Therapy and Antitumor Efficacy.,” 1403:819–38, 2016. https://doi.org/10.1007/978-1-4939-3387-7_47.
Batich, Kristen A., et al. Preconditioning Vaccine Sites for mRNA-Transfected Dendritic Cell Therapy and Antitumor Efficacy. Vol. 1403, 2016, pp. 819–38. Pubmed, doi:10.1007/978-1-4939-3387-7_47.

DOI

Publication Date

2016

Volume

1403

Start / End Page

819 / 838

Related Subject Headings

  • Vaccination
  • Transfection
  • Transcription, Genetic
  • RNA, Messenger
  • Phenotype
  • Neoplasms
  • Mice
  • Immunotherapy
  • Flow Cytometry
  • Electrophoresis